BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2238.p (463 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312742|ref|YP_003776834.1| outer membrane efflux protein [... 207 2e-54 gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 137 2e-33 gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 133 4e-32 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 131 9e-32 gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 130 3e-31 >gi|300312742|ref|YP_003776834.1| outer membrane efflux protein [Herbaspirillum seropedicae SmR1] Length = 492 Score = 207 bits (526), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 154/433 (35%), Positives = 238/433 (54%), Gaps = 7/433 (1%) Query: 31 ELSMPSQWR--APHELAPGEGS-GHTCWWAALGGTELSDLLRRARAGNFELEAAKARVRQ 87 E+ +P +W A ++A E T WW G ELS+L+ + + N+E+ AA +RV+Q Sbjct: 47 EMQVPDKWGGGASADVAHAEQLWPDTDWWKQFGSNELSELVEQGQRNNYEIAAAYSRVKQ 106 Query: 88 ADALASAAGAPLLPELALQ-GYAERTRTASDPSSNAHVLALAMNYELDLWGARQSASRSA 146 A+A A AGAPLLP + G A + +N +L ++YE+D WG ++ +A Sbjct: 107 AEAQARIAGAPLLPSAGINAGAAREIPISGGTPTNTANASLQVSYEIDFWGKNRAGVEAA 166 Query: 147 QQQAAATAYLQEAMKISLDASVAQAYLQTVASRQRAALTRAAIENALQSLAAVNSQYRAG 206 + A Y +E + +++ + + YLQ ++ R R A+ R ++NA + L V +Q RAG Sbjct: 167 EATLRANRYDRETVALTVTSGIVSTYLQVLSLRDRLAIARQNVQNAERVLRLVEAQSRAG 226 Query: 207 HVTATELTRRRRALLALQQQLVSHQRQAAAGLAALAALLGLPVQEVRVSLEGLDTLQVPT 266 + +L R+R AL +Q + Q+Q ALA LLG P Q +V +GL +Q+P Sbjct: 227 SASPLDLARQRSALANQRQLIPDLQQQEQDAQTALAILLGRPPQNFKVEEKGLARIQMPR 286 Query: 267 QQAALPPSLLLRRPDIVAMEHQLAAADADVTIARAALLPSLNLGASLGIGDERW--RLPG 324 A LP LL RRPDI E LAAA+A+V +ARAAL PS+ L S G G Sbjct: 287 ISAGLPSELLTRRPDIRRAEASLAAANANVAVARAALFPSITLTGSTGAQSSALLSLFDG 346 Query: 325 HPVY-ELVAGLTMPIFNGGRLRAEREQRSARRDEVLALYRAGIINALAEVESALQGLDAS 383 ++ L AGL PIF+GG+LR +R+ A+++E++ +YR +I +L+EVE AL + + Sbjct: 347 PNLFANLGAGLIAPIFDGGKLRGQRDLAIAQKEELVQVYRQRVITSLSEVEKALGAIRSL 406 Query: 384 RSERRQQQQALAESSELVRLASSRHAAGFVDVLALDDARAELYAISDVAVEKRLTELEAA 443 R + + ++ LA R+ AG D+L + D + L + + +L L+A Sbjct: 407 EERYRLKVGEVDQARFAFELAEIRYRAGAEDLLVVLDTQRTLSEAQNQLGQIKLQRLQAT 466 Query: 444 VTLYKALGGEWRD 456 V+LYKALGG W+D Sbjct: 467 VSLYKALGGGWQD 479 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 137 bits (346), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 143/468 (30%), Positives = 217/468 (46%), Gaps = 37/468 (7%) Query: 20 ACTLAPEQKHTELSMP----------------SQWRAPHELAPGEGSGHTCWWAALGGTE 63 C++AP + + P SQW+ P E WW G + Sbjct: 30 GCSVAPTYERPSVDTPAAFKEAAPATQTGENGSQWKTAQ---PAEDIARGEWWKIFGDEK 86 Query: 64 LSDLLRRARAGNFELEAAKARVRQADALASAAGAPLLPELALQGYAERTRTA-------S 116 L+ L A+ N +L+AA AR+ QA AL A A P++ A R R + + Sbjct: 87 LNALEDTAQQANQDLKAAAARLGQARALTRDARAGYFPQVDAGVGATRQRPSPASQGLPA 146 Query: 117 DPSSNAHVLALA---MNYELDLWGARQSASRSAQQQAAATAYLQEAMKISLDASVAQAYL 173 D + + L A ++YE+DL+G S +A A + L ++ ++L A VAQ Y Sbjct: 147 DADTRPNTLYRAQVNVSYEVDLFGRVASTVDAATADAQRSEALFHSVLLALQADVAQQYF 206 Query: 174 QTVASRQRAALTRAAIENALQSLAAVNSQYRAGHVTATELTRRRRALLALQQQLVSHQRQ 233 AAL R ++ +SL V ++ G ++ ++ R R L + Q + + RQ Sbjct: 207 LVRELDAAAALYRGTVDLRQESLRLVQRRFDEGDISEVDVARARAELASAQSEALGIARQ 266 Query: 234 AAAGLAALAALLGLPVQEVRVSLEGLDTLQVPTQQAALPPSLLLRRPDIVAMEHQLAAAD 293 A ALA LLG E + + L L V T LP +LL RRPDI A E +AAA+ Sbjct: 267 RATAEHALAILLGRTPAEFSLPPQPLTRLAV-TVPPGLPSALLERRPDIAAAERAMAAAN 325 Query: 294 ADVTIARAALLPSLNLGASLG-----IGD-ERWRLPGHPVYELVA-GLTMPIFNGGRLRA 346 A V +A++A PSLNL LG +GD W + LV L+MP+F+GGR +A Sbjct: 326 ARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSSRTFLLGPLVGTALSMPLFDGGRRQA 385 Query: 347 EREQRSARRDEVLALYRAGIINALAEVESALQGLDASRSERRQQQQALAESSELVRLASS 406 ++ A +E +A YR ++NA EVE L L + R Q +A+A S+ +L+ + Sbjct: 386 GVDRARASYEEQVASYRGTVLNAFREVEDNLANLRILDQQNRAQDEAVAASARAAKLSHT 445 Query: 407 RHAAGFVDVLALDDARAELYAISDVAVEKRLTELEAAVTLYKALGGEW 454 ++ G V ++ DA + +V + V L +ALGG W Sbjct: 446 QYREGAVSYFSVIDADRTVLQQQRASVTLDGERARSTVNLIRALGGGW 493 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 133 bits (334), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 140/437 (32%), Positives = 207/437 (47%), Gaps = 35/437 (8%) Query: 40 APHELAPGEGSGHTCWWAALGGTELSDLLRRARAGNFELEAAKARVRQADALASAAGAPL 99 A H++A S W L L+ +A + +L A RV++A AL A L Sbjct: 51 AAHQVANEAASPPLPWRDYFTDPPLQALITQALENSRDLRTAALRVQEAQALYGIGHADL 110 Query: 100 LPELALQGYAERTRTASDPS-------SNAHVLALAMN-YELDLWGARQSASRSAQQQAA 151 P + Q +R+RT +D + +A+ L ++ +E+DLWG +S +A + A Sbjct: 111 FPTIGAQAGLDRSRTPADLNLTRRPLVGSAYQAGLGLSSWEIDLWGRLRSLDDAALESAR 170 Query: 152 ATAYLQEAMKISLDASVAQAYLQTVASRQRAALTRAAIENALQSLAAVNSQYRAGHVTAT 211 A+ + A+ +SL A VA A+L+ +R + I + +S + + G + Sbjct: 171 ASQATRSAVALSLMAQVADAWLELAELDERLEIANQTIASRQESFRIFSRRVEVGATSRL 230 Query: 212 ELTRRRRALLALQQQLVSHQRQAAAGLAALAALLG----LPVQEVRVSLEGLDTLQVPTQ 267 LT+ L Q Q+Q A L AL L+G LPV + R GLD + P Q Sbjct: 231 NLTQIETLLTQAQALGAQLQQQRAQRLNALTLLVGSPVVLPVGQARA---GLDAVFAPLQ 287 Query: 268 QAALPPSLLLRRPDIVAMEHQLAAADADVTIARAALLPSLNLGASLGIGDER----WRLP 323 LP LL RRPD+ A EH+L AA+A++ ARAA PS++L ++G +R Sbjct: 288 -PGLPALLLTRRPDVAAAEHRLMAANANIGAARAAFFPSISLTGNVGTASAELSGLFRDG 346 Query: 324 GHPVYELVAGLTMPIFNGGRLRAEREQRSARRDEVLALYRAGIINALAEVESALQGLDAS 383 H + +T+PIF GRLR RRD +A Y + A EV AL A+ Sbjct: 347 SH-AWTFSPSITLPIFTAGRLRNNLALSEVRRDLAVAAYEKTVQTAFREVADAL----AA 401 Query: 384 RSERRQQ----QQALAESSELVRLASSRH---AAGFVDVLALDDARAELYAISDVAVEKR 436 R QQ Q A+ E RL++ R+ AA F+DVL DA+ +L + V+ R Sbjct: 402 RQWLTQQLEIAQSAVRVQRERARLSALRYDNGAAPFLDVL---DAQRDLLSAQQQLVQIR 458 Query: 437 LTELEAAVTLYKALGGE 453 L ++V LY ALGGE Sbjct: 459 RALLSSSVALYAALGGE 475 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 131 bits (330), Expect = 9e-32, Method: Compositional matrix adjust. Identities = 131/454 (28%), Positives = 210/454 (46%), Gaps = 19/454 (4%) Query: 20 ACTLAPEQKHTELSMPSQW--RAP-HELAPGEGSGHTCWWAALGGTELSDLLRRARAGNF 76 C P+ ++ MP+ W AP + P + + WW G +L+ L ++A AGN Sbjct: 18 GCASGPDYTRPQVDMPAAWTSEAPWRSMQPADATPRGPWWERFGDAQLNALQQQALAGNQ 77 Query: 77 ELEAAKARVRQADALASAAGAPLLPELALQGYAERTR-TASDPSSNAH-----------V 124 L A AR+ QA A + GA P++ L A R+R +A+ P +N + Sbjct: 78 TLAIATARLAQARAQLNVTGAAQTPQVNLNARAARSRISANRPLTNYNSPNFSTVQNDFA 137 Query: 125 LALAMNYELDLWGARQSASRSAQQQAAATAYLQEAMKISLDASVAQAYLQTVASRQRAAL 184 L +++YE+D +G Q + +A A +A E ++ L A +A Y + Sbjct: 138 LGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAELATNYFNLRELDVELDV 197 Query: 185 TRAAIENALQSLAAVNSQYRAGHVTATELTRRRRALLALQQQLVSHQRQAAAGLAALAAL 244 R AI Q+L +++ G + ++ +++ L + Q+ RQ A A+A L Sbjct: 198 VRRAIALQRQALELATARHDLGATSGLDMAQQQALLDSTLTQIDLLGRQRAQYEHAIATL 257 Query: 245 LGLPVQEVRVSLEGLDTLQVPTQQAALPPSLLLRRPDIVAMEHQLAAADADVTIARAALL 304 G P ++ L + P +P +L RRPDI A E +AAA+A + +A AA Sbjct: 258 TGTPAPAFSIT-PSLTPITPPAIPVGVPSDVLERRPDIAAAERAMAAANAQIGVASAAYY 316 Query: 305 PSLNLGASLGIGDERW-RLPGHP--VYELVAGLTMPIFNGGRLRAEREQRSARRDEVLAL 361 PS L S G+ W L P ++ L + +F+GGRLRA + A + +A Sbjct: 317 PSFMLQPSYGVDSRNWATLFNAPSLLWSLGVSASQSLFDGGRLRAGVDFSQAGYEATVAS 376 Query: 362 YRAGIINALAEVESALQGLDASRSERRQQQQALAESSELVRLASSRHAAGFVDVLALDDA 421 YR ++ A+ EVE + GL A Q Q A+A + ++ +ASSR+ G L + A Sbjct: 377 YRRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVLEIASSRYEGGATPYLDVITA 436 Query: 422 RAELYAISDVAVEKRLTELEAAVTLYKALGGEWR 455 + L A + L +V L KALGG+W+ Sbjct: 437 QQSLLNSERQAAQLMGQRLLVSVFLIKALGGDWQ 470 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 130 bits (326), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 127/465 (27%), Positives = 196/465 (42%), Gaps = 36/465 (7%) Query: 21 CTLAPEQKHTELSMPSQWRAPHELAPGEGSG----------HTCWWAALGGTELSDLLRR 70 LAP+ +P+ W + P GS H W L ++ Sbjct: 29 ANLAPDYTQPAAPVPASWTSQAAAQPAVGSAGPAAGEIDADHLGWREFFLDPRLQQVIAA 88 Query: 71 ARAGNFELEAAKARVRQADALASAAGAPLLPELALQGYAERTRTASDPSSN-----AHVL 125 A N +L A + +A A A P ++ G + +RT ++ S+ +H Sbjct: 89 ALDNNRDLRVAALNIEKARAQYRITDANRYPSISATGTSSASRTPAELSTTGSAAVSHQQ 148 Query: 126 ALAMN---YELDLWGARQSASRSAQQQAAATAYLQEAMKISLDASVAQAYLQTVASRQRA 182 ++ + YELD +G ++ S +A + + + ISL A VA A+L + R Sbjct: 149 SVTVGVSAYELDFFGRLKNLSDAALESYQYQVETRRSTHISLVAEVATAWLTLASDRDLL 208 Query: 183 ALTRAAIENALQSLAAVNSQYRAGHVTATELTRRRRALLALQQQLVSHQRQAAAGLAALA 242 L + + ++ A + G V+ +L + + + + + + Q L AL Sbjct: 209 KLAQDTYASQSRTFALSQRSHDIGTVSGVDLASQEATVESARADVARYASQVEQDLNALR 268 Query: 243 ALLGLPVQEVRVSLEGLDTLQV-PTQQAALPPSLLLRRPDIVAMEHQLAAADADVTIARA 301 L+G + + DT V P A LP +LLRRPD++A EH L +A+AD+ ARA Sbjct: 269 LLVGADIDAALLPAGLPDTASVLPATPAGLPSQVLLRRPDVLAAEHTLKSANADIGAARA 328 Query: 302 ALLPSLNLGASLGIGDER---WRLPGHPVYELVAGLTMPIFNGGRLRAEREQRSARRDEV 358 A PS++L A+ G G + + +PIFN G LRA + RD Sbjct: 329 AFFPSISLTANGGTASRTLGGLFKAGSGSWTFSPSINLPIFNAGSLRASLDSAKITRDID 388 Query: 359 LALYRAGIINALAEVESAL-------QGLDASRSERRQQQQALAESSELVRLASSRHAAG 411 +A Y I A EV AL Q LDA Q +A A S + RL+ +R+ G Sbjct: 389 VANYEKAIQTAFREVADALAVRATLDQRLDA-------QGKATAASEKAFRLSDARYRNG 441 Query: 412 FVDVLALDDARAELYAISDVAVEKRLTELEAAVTLYKALGGEWRD 456 L A+ LY+ + RLTE +TLYK LGG W + Sbjct: 442 VDSYLTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVLGGGWSE 486 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.129 0.361 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,052,944 Number of extensions: 34821 Number of successful extensions: 232 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 184 Number of HSP's successfully gapped: 17 Length of query: 463 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 374 Effective length of database: 1,198,455 Effective search space: 448222170 Effective search space used: 448222170 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)