BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2230.p (490 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferas... 220 2e-58 gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferas... 212 6e-56 gi|300309354|ref|YP_003773446.1| 4-aminobutyrate aminotransferas... 147 2e-36 gi|300312624|ref|YP_003776716.1| acetylornithine aminotransferas... 143 3e-35 gi|300312816|ref|YP_003776908.1| aminotransferase class III prot... 135 9e-33 >gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 456 Score = 220 bits (561), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 138/434 (31%), Positives = 224/434 (51%), Gaps = 14/434 (3%) Query: 35 LQRQARQESNARSYPRRLPLVLKRGAGIYVEDIEGRVFIDCLAGAGTLALGHNHPAVIEA 94 L+ AR ES AR R+P+ + + G+ +D +G FID AG G ++G +P V+EA Sbjct: 31 LELSARTESMARG-GGRMPIAMDQAFGVTFKDPDGNTFIDLSAGVGVSSVGRCNPRVVEA 89 Query: 95 IVALLHDGAALHTLDLTTPVKDRFMQDLLEILPPELSRQARVQFCGPTGADAIEAALKLV 154 I + +H++++ + + + EI+P L R + F G+DA+EAA+K Sbjct: 90 IRK--QSESLMHSMEVNSSKRTELAAKISEIMPDGL-RGDCITFFTQGGSDALEAAVKFA 146 Query: 155 KSATGGGTVLAFQGAYHGMTQGALQLMGSLAPKAPLNGILSGVQFLPYPYDYRCPLGLGG 214 K TG ++AF G YHG+ + L A + + GV PYPY YR P Sbjct: 147 KRVTGRHQIIAFHGGYHGIWNASNALTTGTAYRKGFGPFMGGVIHAPYPYAYRFPFDTSH 206 Query: 215 DAGVRAGLHYIAATLNDPEGGVPKPAGVVVEAIQGEGGVIAAPPAWLAGLRQLTQSAEVP 274 + + Y+ LN P A V+VE +QGEGG + P +L LR+ + Sbjct: 207 KSAEQIAGEYVDYLLNTPYTAADDVAAVIVEPVQGEGGYVPPSPEFLQILRKACDRSGAL 266 Query: 275 LILDEVQSGFGRSGKMFAFEHAGITPDVVVLSKAIGGGLPLSVVVYKEQLDQWKP-GAHA 333 LI+DEVQ+G GR+GKM+A EH+G+ PD++ K IGG +P++ +V + L P G+ Sbjct: 267 LIVDEVQAGAGRTGKMWAVEHSGVKPDMLTFGKGIGGDMPMAGLVMRSDLAAKIPDGSQP 326 Query: 334 GTFRGNQLAMAAGSATMRVLRSE--DLAGQARTKGQRLETALLALRQLHPHIGEVRGRGL 391 TF N ++ A + +L+ DL +A T G + + + P +GEVRGRGL Sbjct: 327 NTFAANSISAAVALTNISILQDPRLDLVNRAHTLGLEAQERIRSFNS--PWVGEVRGRGL 384 Query: 392 MLGVEIIDPQAPADVLGNPAAAPALARALQHECLRRGLILELGGRHGCVVRFLPPLVIGE 451 M+G+E+++ + + P + L + + + + G+++ GR+ V+R +P L I Sbjct: 385 MIGIELVENRETRE----PLSREKLGKLMDY-VVGHGVLMIPCGRYTNVMRVMPSLTIPR 439 Query: 452 DELERVIDIFAQAL 465 + + +DIF L Sbjct: 440 SLMFKGLDIFGAGL 453 >gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 426 Score = 212 bits (539), Expect = 6e-56, Method: Compositional matrix adjust. Identities = 151/440 (34%), Positives = 227/440 (51%), Gaps = 27/440 (6%) Query: 36 QRQARQESNARSYPRRLPLVL----KRGAGIYVEDIEGRVFIDCLAGAGTLALGHNHPAV 91 ++ +Q NA + PR + ++ +R A + D+EGR FID AG L GH HP + Sbjct: 7 NQELQQRKNAAT-PRGVGVMCDFYAERAANAELWDVEGRRFIDFAAGIAVLNTGHRHPKL 65 Query: 92 IEAIVALLHDGAALHTLDLTTPVKDRF-MQDLLEILPPELSRQARVQFCGPTGADAIEAA 150 ++A+ A + HT P + + + L P + F TGA+A+E A Sbjct: 66 LDAMRAQMDK--FTHTAYQIVPYASYVELAERINRLTPGNYPKKTAFFS--TGAEAVENA 121 Query: 151 LKLVKSATGGGTVLAFQGAYHGMTQGALQLMGSLAPKAPLNGILSGVQF-LPYPYDYRCP 209 +K+ ++ TG V+AF G +HG T + L G +AP G G F PYP Sbjct: 122 IKIARAHTGRPGVIAFAGGFHGRTMMGMALTGKVAPYKLGFGPFPGDVFHAPYPSALH-- 179 Query: 210 LGLGGDAGVRAGLHYIAATLNDPEGGVPKPAGVVVEAIQGEGGVIAAPPAWLAGLRQLTQ 269 G+ + + A L + + A +++E +QGEGG AAP ++ GLR L Sbjct: 180 -GITSEDALEA-----VKGLFKSDIEAKRVAAIILEPVQGEGGFYAAPADFMRGLRALCD 233 Query: 270 SAEVPLILDEVQSGFGRSGKMFAFEHAGITPDVVVLSKAIGGGLPLSVVVYK-EQLDQWK 328 + LI DEVQSG+GR+GK+FA EH + PD++ ++K++ GG+PLS V + E +D Sbjct: 234 EHGILLIADEVQSGYGRTGKLFAMEHYDVLPDLMTMAKSLAGGMPLSAVNGRAEIMDAPA 293 Query: 329 PGAHAGTFRGNQLAMAAGSATMRVLRSEDLAGQARTKGQRLETALLALRQLHPHIGEVRG 388 PG GT+ GN LA+A+ A + V+ E L + + G +L+ L LR P I EVRG Sbjct: 294 PGGLGGTYAGNPLAIASALAVLDVMEEEQLVTRGQRLGDKLQEHLKELRSSVPQIAEVRG 353 Query: 389 RGLMLGVEIIDPQAPADVLGNPAAAPALARALQHECLRRGLILELGGRHGCVVRFLPPLV 448 G M+ VE DP G P A + +Q L GL+L G +G V+RFL PL Sbjct: 354 VGAMVAVEFADPAT-----GKPDA--EYTKKVQQHALNNGLLLLTCGSYGNVIRFLFPLT 406 Query: 449 IGEDELERVIDIFAQALRVA 468 I + ++ + I A+A+R+A Sbjct: 407 IPDTVMDEALGILAKAIRLA 426 >gi|300309354|ref|YP_003773446.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 442 Score = 147 bits (370), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 119/423 (28%), Positives = 202/423 (47%), Gaps = 32/423 (7%) Query: 31 THPLLQRQARQESNARSYPRRLPLVLKRGAGIYVEDIEGRVFIDCLAGAGTLALGHNHPA 90 T L++R+ + R P+ +G+++ D +G ++D A ++GH HP Sbjct: 25 TRALIERRRNTFGDGVPLFYRDPVHFVSASGVWLRDQDGLEYLD--AYNNVPSVGHCHPR 82 Query: 91 VIEAIVALLHDGAALHT-LDLTTPVKDRFMQDLLEILPPELSRQARVQFCGPTGADAIEA 149 V+EA+ A L+T + V + + LL LP ELS+ V F +G ++++ Sbjct: 83 VVEAVS---KQAATLNTHTRYLSDVVTSYAERLLGTLPRELSK---VLFTS-SGTESVDL 135 Query: 150 ALKLVKSATGGGTVLAFQGAYHGMTQGALQLMGSLAPKAPLNGILSGVQFLPYPYDYR-- 207 A++L + TGG + + AYHG + ++ + P P+ GI V+ + P YR Sbjct: 136 AMRLARLYTGGSGFIVTRFAYHGHSTAVAEITPAFGPGVPI-GI--NVRLVDAPDGYRQD 192 Query: 208 CPLGLGGDAGVRAGLHYIAATLNDPEGGVPKPAGVVVEAIQGEGGVIAAPPAWLAGLRQL 267 P+G+ +AA + D E + AG++++ + G+ PP +L Sbjct: 193 APVGI-------KFAQDVAAAIADMERHGIRFAGLILDTVCSSDGLFVDPPGFLKDAIAT 245 Query: 268 TQSAEVPLILDEVQSGFGRSGK-MFAFEHAGITPDVVVLSKAIGGGLPLSVVVYKEQLDQ 326 + A I DEVQ GFGR+G+ M+ FE I PD+VV+ K +G G+P++ V + + Q Sbjct: 246 VKKAGGVFIADEVQPGFGRTGEGMWGFERHRIVPDIVVMGKPMGNGMPIAATVTRADIMQ 305 Query: 327 --WKPGAHAGTFRGNQLAMAAGSATMRVLRSEDLAGQARTKGQRLETALLALRQLHPHIG 384 + + TF GN + AA SA + V+ SE L A+ G L+ L L+ H IG Sbjct: 306 AFTERSGYFNTFGGNTVCCAAASAVLDVIESEGLINNAKETGASLKLGLEQLKGQHQCIG 365 Query: 385 EVRGRGLMLGVEIIDPQAPADVLGNPAAAPALARALQHECLRRGLILELGGRHGCVVRFL 444 +VR GL VE + P A +A + + ++ +++ G G +++ Sbjct: 366 DVRAIGLYAAVEFVLPD-------TKTADTDMALHVVNTLRQKRILISTCGPAGNILKIR 418 Query: 445 PPL 447 PPL Sbjct: 419 PPL 421 >gi|300312624|ref|YP_003776716.1| acetylornithine aminotransferase [Herbaspirillum seropedicae SmR1] Length = 400 Score = 143 bits (361), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 120/445 (26%), Positives = 203/445 (45%), Gaps = 71/445 (15%) Query: 39 ARQESNARSYPRRLP-LVLKRGAGIYVEDIEGRVFIDCLAGAGTLALGHNHPAVIEAIVA 97 ++ ++N Y P LV G G+++ D G+ ++D L G LGH + +A+ A Sbjct: 4 SQSDANKLMYVTNRPELVFTEGHGMWLTDHNGKRYLDYLQGWAVNTLGHAPQCIADALAA 63 Query: 98 LLHDGAALHTLDLTTPVKDRFMQDLLEI--LPPELSRQARVQFCGPTGADAIEAALKLV- 154 + L P + + +E+ L S RV F +G +A E A+KL Sbjct: 64 --------QSKKLINPSPAFYNEPSIELAKLLTANSVFDRV-FFANSGGEANEGAIKLAR 114 Query: 155 ----KSATGGGT----VLAFQGAYHGMTQGALQLMGS------LAPKAPLNGILSGVQFL 200 K+ G+ ++ F+ ++HG T + G AP+ P Sbjct: 115 KWGKKNPAADGSARFEIITFKHSFHGRTLATMSASGKDGWDTMFAPQVP----------- 163 Query: 201 PYPYDYRCPLGLGGDAGVRAGLHYIAATLNDPEGGVPKPAGVVVEAIQGEGGVIAAPPAW 260 G V L + A + + V++E +QGEGGVI A + Sbjct: 164 ------------GFPKAVLNDLESVKALIGE------HTVAVMLEPVQGEGGVIPASKEF 205 Query: 261 LAGLRQLTQSAEVPLILDEVQSGFGRSGKMFAFEHAGITPDVVVLSKAIGGGLPLSVVVY 320 + GLR LT+ + LI+DEVQSG GR+G++FA++H+GI PD++ L+K IGGG+PL+ ++ Sbjct: 206 MQGLRSLTKEKNLLLIVDEVQSGMGRTGQLFAYQHSGIEPDIMTLAKGIGGGVPLAALLA 265 Query: 321 KEQLDQWKPGAHAGTFRGNQLAMAAGSATMRVLRSEDLAGQARTKGQRLETALLALRQLH 380 +E++ ++ G GT+ GN L A G A ++ L R +GQ L L + + + Sbjct: 266 REEIACFEAGEQGGTYNGNPLMTAVGVAVIKELLKPGFMESVRERGQYLRQRSLEISEKY 325 Query: 381 PHIGEVRGRGLMLGVEIIDPQAPADVLGNPAAAPALARALQHECLRRGLILELGGRHGCV 440 GE RG GL+ +++ P V P GL+L + Sbjct: 326 GFEGE-RGEGLLRALQLGRDIGPQIVEAARNLEPV------------GLLLN--SPRPNL 370 Query: 441 VRFLPPLVIGEDELERVIDIFAQAL 465 +RF+P L + ++E++++ + + L Sbjct: 371 LRFMPALNVTKEEIDQMFSMLEEVL 395 >gi|300312816|ref|YP_003776908.1| aminotransferase class III protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 135 bits (339), Expect = 9e-33, Method: Compositional matrix adjust. Identities = 126/447 (28%), Positives = 210/447 (46%), Gaps = 40/447 (8%) Query: 49 PR-RLPLVLKRGAGIYVEDIEGRVFIDCLAGAGTLALGHNHPAVIEAIVALLHDGAALHT 107 PR +LP+ + G G+ + D +G+ +ID GA LGH HP VIEAI + + A HT Sbjct: 9 PRSKLPMAVA-GEGMELIDQDGKRYIDASGGAAVSCLGHGHPRVIEAIRKQVGELAYAHT 67 Query: 108 LDLTTPVKDRFMQDLLEILPPELSRQARVQFCGPTGADAIEAALKLVKSAT------GGG 161 TT + L + P L+ V F G++A+EAALKL + Sbjct: 68 SFFTTAPAEELAAMLADAAPGSLNH---VYFL-SGGSEAVEAALKLARQYYVEVGQPQRR 123 Query: 162 TVLAFQGAYHGMTQGALQLMGSLAPKAPLNGILSGVQFLPYPYDYRCPLGLGGDAGVRAG 221 ++A + +YHG T GAL + G+ + +L + Y YR A + Sbjct: 124 HIIARRQSYHGNTLGALAIGGNAWRREMFMPMLIEAHHVSPCYAYR------NRADGESD 177 Query: 222 LHYIAATLNDPEG-----GVPKPAGVVVEAIQGEGGVIAAPPA--WLAGLRQLTQSAEVP 274 + Y+ ++ E G + V E + G A PP + +R + V Sbjct: 178 VQYVQRLADELEQKILSLGADQVIAFVAETVVG-ATAGAVPPVGDYFRKIRAVCDKYGVL 236 Query: 275 LILDEVQSGFGRSGKMFAFEHAGITPDVVVLSKAIGGGL-PLSVVVYKEQL-DQWKPGA- 331 LILDEV SG GR+G +FA E G+ PD+VV++K +G G P+ ++ + + D G+ Sbjct: 237 LILDEVMSGMGRTGYLFACEEDGVVPDIVVIAKGLGAGYQPIGAMICSDHIYDAVLRGSG 296 Query: 332 ---HAGTFRGNQLAMAAGSATMRVLRSEDLAGQARTKGQRLETALLALRQLHPHIGEVRG 388 H T+ G+ A AA A + ++ E L R +G++L + L H+G++RG Sbjct: 297 FFQHGHTYIGHATACAAAVAVQKTIQEERLLENVRQRGEQLRSELRQAFGDQAHVGDIRG 356 Query: 389 RGLMLGVEIIDPQAPADVLGNPAAAPALARALQHECLRRGLIL-----ELGGRHGCVVRF 443 RGL +GVE++ ++ L A +A E ++RGL++ + G++G + Sbjct: 357 RGLFVGVELVAERSSKLPLSPDLRTHARVKA---EAMKRGLLVYPMGGTIDGKNGDHILL 413 Query: 444 LPPLVIGEDELERVIDIFAQALRVACA 470 PP + +++ ++ + A+ + A Sbjct: 414 APPFIASSNDISEIVQRLSDAVAASVA 440 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.139 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,582,806 Number of extensions: 72988 Number of successful extensions: 255 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 230 Number of HSP's successfully gapped: 11 Length of query: 490 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 401 Effective length of database: 1,198,455 Effective search space: 480580455 Effective search space used: 480580455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)