BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2228.p (366 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313764|ref|YP_003777856.1| Fe2+-dicitrate sensor membrane ... 92 7e-20 gi|300313795|ref|YP_003777887.1| transmembrane sensor protein [H... 78 9e-16 gi|300311747|ref|YP_003775839.1| Fe2+-dicitrate sensor membrane ... 68 1e-12 gi|300309380|ref|YP_003773472.1| Fe2+-dicitrate sensor membrane ... 64 2e-11 >gi|300313764|ref|YP_003777856.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 326 Score = 92.0 bits (227), Expect = 7e-20, Method: Compositional matrix adjust. Identities = 99/332 (29%), Positives = 145/332 (43%), Gaps = 32/332 (9%) Query: 29 ALFDTEDPVRVEAARWHTCREQG-CSPQQEAQFQRWLSASTAHGAAYARIEAGLAGLNAA 87 A+ ++ + EAA W +G + ++ +W S H A++R Sbjct: 7 AVDGIDEAILTEAATWLMEMHEGPLDAARRSKLAQWRQRSPEHERAWSRA---------- 56 Query: 88 TPALRRNAASAAPATIQARGASGGSWWQRHMGARWRPRFNALALCCVMLLGCGLATLHWL 147 AL + +A P GG+ + +G R + L + L G H L Sbjct: 57 --ALLQETFAALP--------PGGATALKQVGPAGRRKAIKLLAYALTLGPAGWLAYHAL 106 Query: 148 GQRSFHQSFAVRQGERMELTLPDGTELALDSGTALEVTLLPQRREVHLTSGQAMFHVAHD 207 A GER E+ L DG+ L L++ T + + P +R + L G+ M A D Sbjct: 107 SGPEQGARIATAIGERREVQLDDGSRLWLNTDTRITLDFTPAQRLLRLQRGEIMVLTAPD 166 Query: 208 SS---RPFVVETGATRVTVLGTRFSVRRGNEDAVEVAVEQGHVMVTSTAPDHPAQANLL- 263 RPF+V T + LGTRF+VR + AVEVAV QG V +T P AQ +L Sbjct: 167 RQQPPRPFLVATRQGSLRPLGTRFTVRE-RDQAVEVAVLQGAVEIT---PASSAQQTVLP 222 Query: 264 AGQTVQASASGLLGAVRDIDPASIAPWRRGLLHFTSTPLAQALQEFERYGPSHVFVRDPV 323 +G + + SG A R D A W RGLL PLAQ + E RY S + DP Sbjct: 223 SGMQTRFTRSG-SAAPRPCDDTQAA-WSRGLLIAERMPLAQFIGELGRYR-SGLLQADPA 279 Query: 324 VAAMQVGGSYRLDSPQAFAQTMPRILPVRLVQ 355 +A ++V G + L + + + LPVR+ Q Sbjct: 280 IAGLEVSGVFPLADTDRVLRLLAQTLPVRVQQ 311 >gi|300313795|ref|YP_003777887.1| transmembrane sensor protein [Herbaspirillum seropedicae SmR1] Length = 342 Score = 78.2 bits (191), Expect = 9e-16, Method: Compositional matrix adjust. Identities = 58/180 (32%), Positives = 83/180 (46%), Gaps = 3/180 (1%) Query: 167 TLPDGTELALDSGTALEVTLLPQRREVHLTSGQAMFHVAHDSSRPFVVETGATRVTVLGT 226 TL DG+ L L +A QRR V L G+ + VA D +RPFV++T + LGT Sbjct: 140 TLADGSRLLLSGSSAANFHFDAQRRTVELVQGELLVEVARDPARPFVIQTADGSIRALGT 199 Query: 227 RFSVRRGNEDAVEVAVEQGHVMVTSTAPDHPAQANLLAGQTVQASASGLLGAVRDIDPAS 286 RF VRR + V++ + V + A A L AGQ V+ G G + ID A+ Sbjct: 200 RFLVRR-DRAGTLVSMLESKVSIQPGADTTQAPLVLEAGQRVRLHG-GAAGPLEHIDAAA 257 Query: 287 IA-PWRRGLLHFTSTPLAQALQEFERYGPSHVFVRDPVVAAMQVGGSYRLDSPQAFAQTM 345 + WRR L + PL + L E R+ P + +A + + LD Q Q + Sbjct: 258 LERAWRRHQLVSNARPLTEVLDELNRHRPGRILYDQAQLAGLHMTVVLPLDDTQRALQLL 317 >gi|300311747|ref|YP_003775839.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 342 Score = 67.8 bits (164), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 61/207 (29%), Positives = 94/207 (45%), Gaps = 16/207 (7%) Query: 155 SFAVRQGERMELTLPDGTELALDSGTALEVTLLPQRREVHLTSGQAMFHVAHDSS----R 210 S+A R G++ +L L DGT LAL++ +++++ RR + L G+ D+ R Sbjct: 128 SYATRTGDQRQLRLGDGTALALNTSSSVQLRFDDTRRLLLLRQGEIFIDTGADAQWPHHR 187 Query: 211 PFVVETGATRVTVLGTRFSVRRGNEDAVEVAVEQGHVMVTSTAPDHPAQANLLAGQTVQA 270 PF V+T R+ LGTRF VR+ + D ++ V +G V V D P A G+ V Sbjct: 188 PFWVQTAHARLQALGTRFDVRQ-HGDGTQLTVLEGRVAVHVAGVD-PLVAQ--PGEIVDI 243 Query: 271 SASGLL----GAVRDIDPASIAPWRRGLLHFTSTPLAQALQEFERYGPSHVFVRDPVVAA 326 G L +DP + W L LA + + RY S DP VA Sbjct: 244 HPDGRLVRRAARASAMDPTA---WLEQSLVVRQMRLADVVAQLARY-RSGWLQCDPAVAD 299 Query: 327 MQVGGSYRLDSPQAFAQTMPRILPVRL 353 QV G ++L + + + LP+ + Sbjct: 300 WQVSGVFQLQDTDVALEALAQALPLTI 326 >gi|300309380|ref|YP_003773472.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 336 Score = 63.9 bits (154), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 85/305 (27%), Positives = 128/305 (41%), Gaps = 39/305 (12%) Query: 50 QGCSPQQEAQFQRWLSASTAHGAAYARIEAGLAGLNA-ATPALRRNAASAAPATIQARGA 108 Q +P ++ W SA H A+ RIE+ L A A+P NAA A A Sbjct: 34 QPLAPSTLEAWEAWRSAHPDHERAWRRIESVRGRLQALASPV---NAALAQAALT----P 86 Query: 109 SGGSWWQRHMGARWRPRFNALALCCVMLLGCGLATLHWLGQRS-----FHQSFAVRQGER 163 SG R + +++ G G+A W RS + G++ Sbjct: 87 SGN-------------RRQVIKTLALLVFGGGVA---WGLTRSEPWQQWRADLRTDIGQQ 130 Query: 164 MELTLPDGTELALDSGTALEVTLLPQRREVHLTSGQAMFHVAH-DSSRPFVVETGATRVT 222 L L DG++L L++ +A+ + + R + L SG+ + RPF+VET Sbjct: 131 RRLVLEDGSQLVLNTHSAVNLAFSAEERRLRLISGELLVTTGKAGGGRPFLVETAQGLAY 190 Query: 223 VLGTRFSVRRGNEDAVEVAVEQGHVMVTSTAPDHPAQAN--LLAGQTVQASASGLLGAVR 280 LGTR++VR+ EV+V +G V + P AQ L AGQ SA G +G V Sbjct: 191 ALGTRYAVRQ-RAGRTEVSVWEGAVRLV---PRQAAQQARVLQAGQQAAFSAQG-VGDVT 245 Query: 281 DIDPASIAPWRRGLLHFTSTPLAQALQEFERYGPSHVFVRDPVVAAMQVGGSYRLDSPQA 340 D +A W G++ L L E RY + P V+ +++ GSY + A Sbjct: 246 TADETRMA-WTDGVIVARGMRLDAFLAELGRYTTDRLSC-APAVSGVRISGSYPVADSDA 303 Query: 341 FAQTM 345 T+ Sbjct: 304 VIATL 308 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.130 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,107,621 Number of extensions: 45742 Number of successful extensions: 216 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 207 Number of HSP's successfully gapped: 4 Length of query: 366 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 280 Effective length of database: 1,212,660 Effective search space: 339544800 Effective search space used: 339544800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)