BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2211.p (192 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311658|ref|YP_003775750.1| non-ribosomal peptide synthetas... 65 3e-12 >gi|300311658|ref|YP_003775750.1| non-ribosomal peptide synthetase [Herbaspirillum seropedicae SmR1] Length = 9160 Score = 65.5 bits (158), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 62/201 (30%), Positives = 86/201 (42%), Gaps = 20/201 (9%) Query: 1 MQPQGPYHLLGWSLGGLLALEIASQLESRGQSVAWVGAVDAKSPQQRALDR---LGKNRE 57 +QPQGPYHLLGWSLG LA ++A +LE++G VA++G VD PQ A D L ++ Sbjct: 8941 VQPQGPYHLLGWSLGATLAAQVAQRLEAQGTQVAFLGLVDGYVPQSEAADARDWLDDLQD 9000 Query: 58 LVRRTQPS----AQEMERFWTSQA--ERDDGAAQFLADVQPQSRVAAQDQQRLAENLARA 111 V P+ A +R+ QA R GA A + L +L A Sbjct: 9001 FVSVMLPASWDGAAGSKRWLALQALQARQSGALPSEAAALAMLSAMLEQAGELPASLPSA 9060 Query: 112 PNYVMALTSAGLERRIRADL----------HVWWSAQTPPQQRQGWEDSSTGTMSVNHIA 161 A R+I A H WW A+ P R + T + Sbjct: 9061 GELARIFVVARHLRQISAQAPALEAVAASPHYWWIARRKPAHRAELKRQLGQTGCAEVLL 9120 Query: 162 QVSHREIVKSPQLVD-AIQAL 181 + H I+K +L+D +QAL Sbjct: 9121 ETDHFSIMKQARLLDEVVQAL 9141 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.127 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 572,095 Number of extensions: 21473 Number of successful extensions: 90 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 90 Number of HSP's successfully gapped: 1 Length of query: 192 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 112 Effective length of database: 1,241,070 Effective search space: 138999840 Effective search space used: 138999840 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)