BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2210.p (196 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312216|ref|YP_003776308.1| IS1404 transposase [Herbaspiril... 83 1e-17 >gi|300312216|ref|YP_003776308.1| IS1404 transposase [Herbaspirillum seropedicae SmR1] Length = 367 Score = 83.2 bits (204), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 52/144 (36%), Positives = 70/144 (48%), Gaps = 6/144 (4%) Query: 54 IDQATRRCLALHVARQI---RSNDVIDIQAEAMVTYGVPAYLRSDNGPEMVAKNLRRWLA 110 +D T+ +A+ V I R V+D A+ +P +R D+GPE + L W A Sbjct: 227 VDDFTKESVAIEVDTSISGLRLGRVLDKVAQER---PLPTMIRVDHGPEFTSLALDAWAA 283 Query: 111 SVGSKTLYIEPGSPWENGYCESFNGKFGDELLNGEIFYTLREAQVIIERWRHQYNTVRPH 170 G K + +PG P +N Y ESFNG+F DE LN +F TL EA+ IE WR Q R Sbjct: 284 GRGVKLAFTQPGKPTQNAYIESFNGRFRDECLNDHLFSTLHEARYRIEAWRAQRTQRRTA 343 Query: 171 SSLKYRPPAPQATLPASTPAPLRL 194 + P A A + P RL Sbjct: 344 QLQRQESPPKTAYQTAKSYIPPRL 367 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 620,394 Number of extensions: 24046 Number of successful extensions: 93 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 93 Number of HSP's successfully gapped: 1 Length of query: 196 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 116 Effective length of database: 1,241,070 Effective search space: 143964120 Effective search space used: 143964120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)