BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2196.p (418 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311746|ref|YP_003775838.1| iron-regulated membrane protein... 620 e-179 gi|300311804|ref|YP_003775896.1| iron-regulated membrane protein... 245 6e-66 gi|300310446|ref|YP_003774538.1| sulfite reductase, flavoprotein... 75 8e-15 >gi|300311746|ref|YP_003775838.1| iron-regulated membrane protein [Herbaspirillum seropedicae SmR1] Length = 435 Score = 620 bits (1598), Expect = e-179, Method: Compositional matrix adjust. Identities = 297/407 (72%), Positives = 343/407 (84%) Query: 4 SLAAWLCRHAPRRQHLVLMHRWNGLVIALFLLVASITGSLLVFEDELELWLAPELHLAQP 63 SL WL RHAP R LVL+HRW GL +A FL+V S+TGSLLVF+DEL+ WLAP+L LAQP Sbjct: 7 SLRDWLVRHAPSRHVLVLLHRWTGLGMAFFLIVTSLTGSLLVFDDELDAWLAPQLQLAQP 66 Query: 64 AAGQSATALLDPYQLRQRVAQELAPRAQLNQLMLHPQPGRTVRFLVEPAMDPASGRPYVL 123 A GQSA A++DPY LR++VAQ + P+ ++QL+LH +PGRTV F +PA DPA+G+PY L Sbjct: 67 APGQSAQAMMDPYLLREKVAQAMGPQVHISQLVLHARPGRTVSFQADPATDPATGKPYRL 126 Query: 124 GYDEIWVNPYTGAIQGRRDRQKISLKPAELMPFIFNLHHSLALPRVAGALLMGTVATLWT 183 GYDEI VNPYTG +QG RDR KISLKPA+LMPF+F LHHSLALPR GALL+GTV LWT Sbjct: 127 GYDEILVNPYTGVVQGVRDRDKISLKPADLMPFLFKLHHSLALPRAPGALLLGTVGLLWT 186 Query: 184 LDCLVGLILTWPRARPFLVKWKPAWLIKWQAGFYRVNLDLHRACALWLWVVLLLLAWSSV 243 LDC VG+ILTWPRARPFLVKWKPAWLIKW+AGFYRVNLDLHRA LW WV+LLL AWSSV Sbjct: 187 LDCFVGMILTWPRARPFLVKWKPAWLIKWRAGFYRVNLDLHRAFGLWCWVMLLLFAWSSV 246 Query: 244 MFNLRDQIYLPVMSRLLPFDTSWRGPARLPQPMAQPPMDWPQAHAMARQAMAGFARERGL 303 M NLRDQIYLPVMSR+L FDTSWR LPQ ++ PP WP+AHA+AR+ M GFAR+RGL Sbjct: 247 MLNLRDQIYLPVMSRILSFDTSWRPGPPLPQALSGPPQSWPEAHALARRTMDGFARQRGL 306 Query: 304 HIDFEDRLSFDRRRGLYAYMVHSNADLRSDAGNTGLLIDARSGQILGHWLPTGDRSGNTF 363 IDFE++L FDRRRG+Y Y+VHSNADLR+D GNTGL IDAR+G+ILGHWLP GDR GNTF Sbjct: 307 VIDFEEKLDFDRRRGVYIYLVHSNADLRADVGNTGLRIDARTGKILGHWLPVGDRDGNTF 366 Query: 364 SNWIGALHMGQVWGLPWRIFLAALGLVVSLLTVTGIVIWWKKRAARR 410 SNW+GALHMG V+GLPWRI L +G VSLLTVTG+V+WWKKRAARR Sbjct: 367 SNWLGALHMGHVFGLPWRILLVLVGGAVSLLTVTGVVVWWKKRAARR 413 >gi|300311804|ref|YP_003775896.1| iron-regulated membrane protein [Herbaspirillum seropedicae SmR1] Length = 416 Score = 245 bits (625), Expect = 6e-66, Method: Compositional matrix adjust. Identities = 149/405 (36%), Positives = 232/405 (57%), Gaps = 24/405 (5%) Query: 16 RQHLVLMHRWNGLVIALFLLVASITGSLLVFEDELELWLAPELHLAQPAAGQSATALLDP 75 R LVL+HRW GL A+FL +A +TG+++ ++ EL+ WL P+L A+ A A A + Sbjct: 2 RATLVLLHRWFGLAAAVFLFIAGLTGAVISWDHELDAWLNPQLFDARSATQPGAGAPMPG 61 Query: 76 YQLRQRVAQELAPRAQLNQLMLHPQPGRTVRFLVEPAMDPASGRPYVLGYDEIWVNPYTG 135 +L +RV Q PR ++ ++ +PG T+ +VEP +DPA+G+ Y L Y++I V+P+T Sbjct: 62 LELARRVEQA-EPRLRVTYVLTQAEPGHTIGMMVEPRVDPATGKLYTLDYNQIAVDPFTA 120 Query: 136 AIQGRRDRQKISLKPAELMPFIFNLHHSLALPRVA----GALLMGTVATLWTLDCLVGLI 191 IQGRR ISL L+PF++ LH+++ LP + G LLMG + +W LDCL+ L Sbjct: 121 EIQGRRMWGAISLSRENLLPFLYKLHYTMHLPDLGGLETGILLMGIIGIVWALDCLIALY 180 Query: 192 LTWPRARPFLVKWKPAWLIKWQAGFYRVNLDLHRACALWLWVVLLLLAWSSVMFNLRDQI 251 L++P R W+ + +W+ G +++N DLHR+ +W+W +LL+LA +SV NL Q+ Sbjct: 181 LSFPNWR----SWRKSLAFRWRDGGHKLNFDLHRSGGVWVWGLLLILAVTSVSMNLERQV 236 Query: 252 YLPVMSRL-----LPFDTSWRGPARLPQPMAQPPMDWPQAHAMARQAMAGFARERGLHID 306 P++ L PFDT R P P+ +A+P + QA A+ + AR+ GL + Sbjct: 237 MRPLLQTLSTLTPSPFDT--RTPT-APEKVAEPRITREQAVALGQAQ----ARKMGL-TE 288 Query: 307 FEDRLSFDRRRGLY--AYMVHSNADLRSDAGNTGLLIDARSGQILGHWLPTGDRSGNTFS 364 + + G+Y Y N GN L DA SG G +P +G+ F Sbjct: 289 PVGGVFYSGMFGVYGVGYFQPGNDHGDIGLGNAWLYFDANSGAYEGGKIPGRGSAGDIFL 348 Query: 365 NWIGALHMGQVWGLPWRIFLAALGLVVSLLTVTGIVIWWKKRAAR 409 LH G++ GLP RI ++ +G +V++L+VTG++IW +KR AR Sbjct: 349 QAQFPLHSGRILGLPGRILISFMGALVAMLSVTGVIIWVRKRKAR 393 >gi|300310446|ref|YP_003774538.1| sulfite reductase, flavoprotein component [Herbaspirillum seropedicae SmR1] Length = 850 Score = 75.5 bits (184), Expect = 8e-15, Method: Compositional matrix adjust. Identities = 101/394 (25%), Positives = 171/394 (43%), Gaps = 45/394 (11%) Query: 22 MHRWNGLVIALFLLVASITGSLLVFEDELELWLAPELHLAQPAAGQSATALLDPYQLRQR 81 +H + G+ L+ +TG+LL F +E+ L+P + Q Q AT L P Q+ ++ Sbjct: 9 LHWFIGITAGTVLMTIGVTGALLAFREEIMDLLSPGVMHVQ--VRQQAT--LTPQQVLEK 64 Query: 82 VAQELAPRAQLNQLMLHPQPGRTVRFLVEPAMDPASGRPYVLGYDEIWVNPYTGAIQGRR 141 + QE P+ ++ + L +PG +VR + P G+ +V+PYTGA+ Sbjct: 65 LRQE-EPQRRITTMTLSAEPGDSVRVVFAPPPGERRGQAR-------YVDPYTGAL---- 112 Query: 142 DRQKISLKPA---ELMPFIFNLHHSLALPRVAGALLMGTVATLWTLDCLVGLILTWPRAR 198 L P E F H L LP G +++GT+A + L GL L WPR R Sbjct: 113 ------LPPVTGMEFFEFTERFHRWLLLPTEIGKIVLGTLALCLLMLALSGLYLRWPR-R 165 Query: 199 PFLVKWKPAWL-IKWQAGFYRVNLDLHRACALWLWVVLLLLAWSSVMFNL---RDQI-YL 253 ++ AWL + +Q + +LH W V+ ++ A + + L +D + L Sbjct: 166 ALSLR---AWLRLDFQLQGRSLLWNLHSVIGTWSLVMYVIFATTGAYWALDWFKDGVNTL 222 Query: 254 PVMSRLLPFDTSWRGPARLPQ-PMAQPPMDWPQAHAMARQAMAGFARERGLHIDFEDRLS 312 +R P RG + P Q P+ QA F + + + RL Sbjct: 223 AGETRRAPMA---RGEGKKKDGPNNQTPVALDLT-----QAWNAFLTKAPDYQSAQLRLP 274 Query: 313 FDRRRGLYAYMVHSNADLRSDAGNTGLLIDARSGQILGHWLPTGDRSGNTFSNWIGALHM 372 + L + + +A + + +++ +SG+I +G F I LHM Sbjct: 275 EQGTQPLQIFFLGHDAP--HERARSRMVVLPQSGEIKQLERYEDKSTGGRFIAAIYPLHM 332 Query: 373 GQVWGLPWRIFLAALGLVVSLLTVTGIVIWWKKR 406 G +GLP RI +A L++ L +TG +++ +R Sbjct: 333 GTYFGLPGRIIMAIASLMMPLFGITGWMLYLDRR 366 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.139 0.466 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,417,223 Number of extensions: 61648 Number of successful extensions: 255 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 250 Number of HSP's successfully gapped: 3 Length of query: 418 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 330 Effective length of database: 1,203,190 Effective search space: 397052700 Effective search space used: 397052700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)