BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2184.p (381 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313717|ref|YP_003777809.1| cobalt-zinc-cadmium resistance ... 123 2e-29 gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprote... 88 1e-18 gi|300313894|ref|YP_003777986.1| RND family efflux transporter M... 78 1e-15 gi|300313899|ref|YP_003777991.1| RND family efflux transporter M... 70 2e-13 gi|300312743|ref|YP_003776835.1| membrane-fusion protein [Herbas... 69 8e-13 >gi|300313717|ref|YP_003777809.1| cobalt-zinc-cadmium resistance cation efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 396 Score = 123 bits (309), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 86/290 (29%), Positives = 148/290 (51%), Gaps = 4/290 (1%) Query: 80 LPASVEADPARLAKILPPTTGRIVSIQKRLGDAVKAGDVLFEIESSDLAQAVSDERKAQS 139 LP + + R A ++P G + S+ +LG V+ G VL I SS +++ S+ A+ Sbjct: 86 LPGEIRFNEDRTAHVVPQVAGIVESVSAKLGQKVRKGQVLAVINSSAVSEQRSEMLNAEQ 145 Query: 140 TLALTRRNLERQRSLDQANISARRDLEQAQSDYEQADSEAARAHARVAALGGTAASGGSA 199 LAL R +R++ L + ISA +D QAQ+ +A+ A ++ A+G TA+SGG Sbjct: 146 RLALARTAYQREKQLWEQKISAEQDYLQAQAALREAEVAVRNAQQKLRAIGATASSGGLN 205 Query: 200 RLAVRAPVSGHVIELNAAVGSFWNDNTASVMTVADLSSVYVTASAQEKDLARLHIGQDAE 259 R +RAP G V+E + +G ++ A+ ++DLSSV+ KD+ + G+ Sbjct: 206 RYEIRAPFDGVVVEKHLGLGEAVKED-ANAFLISDLSSVWAEIIVSPKDMQFVRTGEKVT 264 Query: 260 IALDAYPGQPLQAKVKSVGQVLDPDTRTVKVRILVTNTDERLKPGMFAQA-TLREQAHAG 318 + A+ K+ VG ++ +RT K +++ N D+ +PG+F + ++A Sbjct: 265 VRATAFDASA-SGKISYVGSLIGEQSRTAKAHVILPNPDDSWRPGLFVNVEVVSDEAQVP 323 Query: 319 LLVPMTAVVQSGFANRVFVEVAPWKFEARKIMLGAQVGNDVEVTDGLQAG 368 + V A+ + +FV V P F A++++ G G VE+ GL AG Sbjct: 324 VAVSAEALQTVEEKSVIFVRV-PGGFAAQQVVTGRSNGKFVEIRSGLNAG 372 >gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprotein A [Herbaspirillum seropedicae SmR1] Length = 402 Score = 87.8 bits (216), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 102/333 (30%), Positives = 154/333 (46%), Gaps = 42/333 (12%) Query: 69 IEQRSIAIPFSLPASVEADPARLAKILPPTTGRIVSI-QKRL---GDAVKAGDVLFEIES 124 I + +AI LP VEA R+A++ R+ I QKR+ G VK G VLF+I Sbjct: 44 IAPQRVAITNELPGRVEA--FRVAQV----RARVAGIVQKRVFKEGSEVKEGQVLFKI-- 95 Query: 125 SDLAQAVSDERKAQSTLALTRRNL-------ERQRSLDQANISARRDLEQAQSDYEQADS 177 D AQ + AQ+TLA + NL +R + L + N ++++ + A + +QA++ Sbjct: 96 -DPAQYKASLESAQATLARAQANLTQTTLTAQRYKPLVEVNAVSKQEYDNAVAAQKQAEA 154 Query: 178 EAARAHARVAALGGTAASGGSARLAVRAPVSGHVIELNAAVGSFWNDNTASVMT-VADLS 236 + A A V AS V +P+SG + G+ A+ + V L Sbjct: 155 DVAAGKAAV-----QTASLNLGYATVTSPISGRIGRALVTEGALVGQGDATQLALVQQLD 209 Query: 237 SVYVTASAQEKDLARLH----------IGQD-AEIALDAYPGQ--PLQAKVKSVGQVLDP 283 VYV + DL +L +G + A + L+ GQ PL K+ +D Sbjct: 210 PVYVNVTQSSADLLKLQQAMAAGQLKSVGDNKASVTLELENGQAYPLPGKLLFSDISVDE 269 Query: 284 DTRTVKVRILVTNTDERLKPGMFAQATLREQA--HAGLLVPMTAVVQSGFANRVFVEVAP 341 +T +V +R N L PG + +A + EQA LLVP AVV+ + V V A Sbjct: 270 NTGSVSLRAEFPNPKRLLLPGTYVRAKI-EQAVDEQALLVPQQAVVRDAGGSTVMVVNAE 328 Query: 342 WKFEARKIMLGAQVGNDVEVTDGLQAGTRVVVK 374 K E R + G GN+ +V DGL+ G RV+V+ Sbjct: 329 NKVEPRPVKTGGARGNNWQVLDGLKEGDRVIVE 361 >gi|300313894|ref|YP_003777986.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 428 Score = 77.8 bits (190), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 94/316 (29%), Positives = 147/316 (46%), Gaps = 39/316 (12%) Query: 89 ARLAKILPPTTGRIVSIQKRL---GDAVKAGDVLFEIESSDLAQAVSDER----KAQSTL 141 A LA + P G I IQKRL G VKAG L++I+ + A R KA++TL Sbjct: 71 AYLAADIRPQVGGI--IQKRLFKEGSTVKAGQPLYQIDPASYQAAYDSARASLLKAEATL 128 Query: 142 ALTRRNLERQRSLDQANISARRDLEQAQSDY--EQADSEAARAHARVAALGGTAASGGSA 199 A ++ ER L++ + +++D + AQS QAD E +A + AA+ Sbjct: 129 ASSKLKAERYAELNKIDAVSKQDNDDAQSTLRQNQADVEVQKAAVQTAAIN-------LQ 181 Query: 200 RLAVRAPVSGHVIELNAAVGSFWN-DNTASVMTVADLSSVYVTASAQEKDLARLH----- 253 + +P+SG + + G+ T ++ TV + +YV + DL RL Sbjct: 182 YTKILSPISGRIEKSAVTPGALVTASQTTALTTVQQIDPIYVDVTQSTVDLLRLRRELAS 241 Query: 254 -----IGQDA---EIALDAYPGQPLQAKVKSVGQVLDPDTRTVKVRILVTNTDERLKPGM 305 G++A +I L+ K++ G +D T + +R +V N D L PG Sbjct: 242 GQIRKSGENAAQVKIVLEDGSTYERPGKLEFTGLSVDTGTGMITLRAVVPNPDGVLLPGA 301 Query: 306 FAQATLRE--QAHAGLLVPMTAVV--QSGFANRVFVEVAPWKFEARKIMLGAQVGNDVEV 361 + +A L E HA LL P AV Q+G A V A K + R + + VGN+ V Sbjct: 302 YVRAVLEEGVDDHA-LLAPQKAVSRDQTGAAT-ALVLGADGKVQQRLVSVARAVGNNWWV 359 Query: 362 TDGLQAGTRVVVKDGV 377 + GL+ G +++V DG+ Sbjct: 360 SRGLKEGDKLIV-DGL 374 >gi|300313899|ref|YP_003777991.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 406 Score = 70.5 bits (171), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 79/311 (25%), Positives = 140/311 (45%), Gaps = 20/311 (6%) Query: 70 EQRSIAIPFSLPASVEADPARLAKILPPTTGRIVSIQKRLGDAVKAGDVLFEIESSD--- 126 E +++A+P +L V++ I + G + K +G VK G++L E+++ + Sbjct: 80 EGQTLALPGTLQGFVQS------PISARSGGYLKRWTKDIGAEVKKGELLAELDTPEIDQ 133 Query: 127 -LAQAVSDERKAQSTLALTRRNLERQRSLDQANISARRDLEQAQSDYEQADSEAARAHAR 185 L QA++ +A S++ L R ++ R +L + + ++++L++ +S + QA + A A A Sbjct: 134 QLTQALAAREQAASSMELARSSMARWEALRKKDAVSQQELDERRSAFAQASANLAAADAN 193 Query: 186 VAALGGTAASGGSARLAVRAPVSGHVIELNAAVGSFWNDNTAS---VMTVADLSSVYVTA 242 V L G R+ AP SG + N VG + V++ D VYV Sbjct: 194 VGRLRQVE---GFKRIV--APFSGIITRRNVDVGDLIDPGAGRALFVLSQTDPLRVYVNV 248 Query: 243 SAQEKDLARLHIGQDAEIALDAYPGQPLQAKVKSVGQVLDPDTRTVKVRILVTNTDERLK 302 +L + GQ + G+ + KV +D TR+++V + ++N D L Sbjct: 249 PQSYANLVK--PGQQVTVTQSELRGRKFEGKVARTAGAIDAATRSMQVEVTLSNADHALL 306 Query: 303 PGMFAQATLREQAHAGLLVPMTAVVQSGFANRVFVEVAPWKFEARKIMLGAQVGNDVEVT 362 PG F Q L + LLV ++ V + +K+ LG G VEV Sbjct: 307 PGAFVQVALPIKPSGALLVAADTLMFRRDGTLVAAVDPHNQVRLKKLRLGRNFGQTVEVL 366 Query: 363 DGLQAGTRVVV 373 DGLQ +++ Sbjct: 367 DGLQGDEHLIL 377 >gi|300312743|ref|YP_003776835.1| membrane-fusion protein [Herbaspirillum seropedicae SmR1] Length = 402 Score = 68.6 bits (166), Expect = 8e-13, Method: Compositional matrix adjust. Identities = 82/299 (27%), Positives = 132/299 (44%), Gaps = 28/299 (9%) Query: 100 GRIVSIQKRLGDAVKAGDVLFEIESSDLAQAVSDERKAQSTLALTRRNLERQRS-LDQAN 158 G+I + +G+ VKAG +L + + + + + R +TLA + L Q + LD A Sbjct: 92 GQISDVDVVVGETVKAGKLLATVAPAVQSSRIENNR---ATLARLKAELAGQNAQLDFAQ 148 Query: 159 ISARRDLE-QAQSDYEQADSEAARAHARVAA--LGGTAASGGSARLAVR----------- 204 + +R + +A++ + E++R + AA + T A A+R Sbjct: 149 LQFQRQTQLKAENATREESYESSRMNMYAAAARVDATNAQIQQTEAAIREDEAVQKQTRI 208 Query: 205 -APVSGHVIELNAAVGSFWNDNTASVMTVADLSSVYVTASAQEKDLARLHIGQDAEIALD 263 APVSG ++ LNA G N ++M +ADLS + V E+D+ RL G A Sbjct: 209 EAPVSGTIVTLNARPGQMVAANQEALMRIADLSRMTVQVPVAEEDVTRLQKGMTAYFTTP 268 Query: 264 AYPGQPLQAKVKSVGQVLDPDT-RTVK-----VRILVTNTDERLKPGMFAQATL-REQAH 316 YPG+ K++ + + D+ R K V V N L GM A +A Sbjct: 269 GYPGKRWSGKLRQIMLLPTDDSGRQGKKAFYTVFFDVANPSRELMSGMSADVYFVLARAE 328 Query: 317 AGLLVPMTAVVQSGF--ANRVFVEVAPWKFEARKIMLGAQVGNDVEVTDGLQAGTRVVV 373 +P T V + G V V +A K E RKI +G + +V GL+ G +V++ Sbjct: 329 HVPTIPRTLVTKPGMDGTQTVKVVLADGKLETRKIKIGIRDNERAQVLSGLKEGEQVLL 387 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.130 0.352 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 959,900 Number of extensions: 35614 Number of successful extensions: 197 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 183 Number of HSP's successfully gapped: 12 Length of query: 381 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 294 Effective length of database: 1,207,925 Effective search space: 355129950 Effective search space used: 355129950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)