BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2182.p (493 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311703|ref|YP_003775795.1| ABC-type export system outer me... 274 1e-74 gi|300309611|ref|YP_003773703.1| outer membrane channel lipoprot... 217 1e-57 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 169 4e-43 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 166 3e-42 gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 161 1e-40 >gi|300311703|ref|YP_003775795.1| ABC-type export system outer membrane channel protein [Herbaspirillum seropedicae SmR1] Length = 489 Score = 274 bits (700), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 169/461 (36%), Positives = 258/461 (55%), Gaps = 4/461 (0%) Query: 31 GGCAVGPDFKQPAAPQVSAYTAEHI---EASQAIDGQRFVAGQDVAGQWWKAFGSPVLDS 87 GC VGPDF P P+VS YT + + S+ Q+ + G++ +WW+ FGSP LD+ Sbjct: 22 AGCLVGPDFVAPTPPRVSQYTRDPVLLDRMSEPDMSQQLLMGENPPARWWQLFGSPELDA 81 Query: 88 LVDDALAHNADLQAAQAALRAARETAASQSGSLLPSVDLHFMPTRQKVADTLASPLADNS 147 V AL N LQAA + L A + + P +D + M R + A + A + Sbjct: 82 AVTQALTRNPTLQAAVSNLLLAEDNLRAGGAVFYPRLDANMMAERTRSASLVQGSSAPDK 141 Query: 148 YIYNLHTAQLNIAYSPDIFGGTRRQIEAGRAQAEVQRFQRNATYLTLISNVVLAVVNEAA 207 ++++ T ++Y+ D+FG RR +E A +VQ+ +A YLTL +NVV A + A Sbjct: 142 -VFSVATLSGAVSYAIDLFGEKRRTLEQLEAMVDVQQQLHHAAYLTLTANVVNAYIARAG 200 Query: 208 LREQLQAAEKAEALATQQLELVRQQRALGALGLADVAAQESLLAQAQAVLPPLRKQLAQQ 267 Q+ + AL QLE ++ + G LAD+ +Q SLLA QA+L L ++L Q Sbjct: 201 YLSQISLVRQIIALEELQLEAIQARVDTGVAPLADLLSQASLLAGNQALLAQLTQRLYQI 260 Query: 268 RDQIAVLVGRFPGQGGTPTLALSALKLPSELPLSLPSTLVQQRPDVRAAQAQWQAASAQV 327 +A L G FP Q P L L+ L LPS++P+SLPS LV++RPD+ AA+A+ AASA + Sbjct: 261 EHLLAQLQGEFPAQASLPELVLADLHLPSDIPVSLPSALVRRRPDILAAEARMHAASAAI 320 Query: 328 GVAIANRLPAFNITGTIGSSAQDISKLFTSGPGFWTLGLDLATPIFHGGSLQHQQRSAEA 387 G++ A + P+ +T T G +A+++S+L W+ G L+TP+F+GG L+ Q+ +A Sbjct: 321 GMSSAAQYPSLELTATYGVAARNLSQLTGPATSLWSFGPSLSTPLFNGGRLRAQRDAAVN 380 Query: 388 GLEQASAQYRSVVLGAFQNVADTLHAMAADADALKAAARAEDAAQRSFDAAQAQWKAGLA 447 E + ++Y+ VL AF+ VAD L A+ AD + A + A A + +A + AGL+ Sbjct: 381 AFEASQSEYQQTVLTAFEQVADALRALQADGHIVSAQSAAARYAYDTLLLQRASYSAGLS 440 Query: 448 GYPQVLLAQQNLLTASQSRVQAQAGRLADTVALFQALGGGW 488 Y +LL + +A+ + +QA R DTVAL A GGGW Sbjct: 441 SYADMLLYEVQYRSAAIASLQATVQRYQDTVALLVASGGGW 481 >gi|300309611|ref|YP_003773703.1| outer membrane channel lipoprotein [Herbaspirillum seropedicae SmR1] Length = 493 Score = 217 bits (553), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 173/458 (37%), Positives = 247/458 (53%), Gaps = 8/458 (1%) Query: 32 GCAVGPDFKQPAAPQVSAYTAEHIEASQAIDG--QRFVAGQDVAGQWWKAFGSPVLDSLV 89 GCA GP + PA P+ Y A+ A +G QRFV GQ QWW+A+GSP L + V Sbjct: 20 GCAFGPTGEAPAMPRPDHYAAQATPADVGPEGARQRFVLGQRAVPQWWQAYGSPELSAWV 79 Query: 90 DDALAHNADLQAAQAALRAAR-ETAASQSGSLLPSVDLHFMPTRQKVADTLASP-LADNS 147 ++AL +N L A+ L +AR + A SL PSVD RQ+ L P + + Sbjct: 80 EEALRNNHSLAQAERNLASARLQLRAQIDQSLWPSVDAVGQAQRQRA---LGLPNIGPTT 136 Query: 148 YIYNLHTAQLNIAYSPDIFGGTRRQIEAGRAQAEVQRFQRNATYLTLISNVVLAVVNEAA 207 +YN+ Q+ +Y+ D+FG R A AQ + Q +Q +A L++NVV++ +N AA Sbjct: 137 NLYNVFAGQIRASYTFDLFGTERYANAATAAQVDAQSYQFDAARRALVANVVISAINAAA 196 Query: 208 LREQLQAAEKAEALATQQLELVRQQRALGALGLADVAAQESLLAQAQAVLPPLRKQLAQQ 267 LR Q+ E+ ALA V+++ LGA D+ A + +A LP L+ Q Sbjct: 197 LRVQVSDTERLVALAEADASEVQRRAELGAASQDDLLAARANAQSLRASLPGLQAQWLAA 256 Query: 268 RDQIAVLVGRFPGQGGTPTLALSALKLPSELPLSLPSTLVQQRPDVRAAQAQWQAASAQV 327 R +AVL+GR P Q L L++L+LP+E+PL++PS L++QRPD+ AA A + A+AQ Sbjct: 257 RHALAVLMGRTPDQAPA-DLDLASLRLPAEVPLAVPSELLRQRPDILAADAALKVAAAQQ 315 Query: 328 GVAIANRLPAFNITGTIGSSAQDISKLFTSGPGFWTLGLDLATPIFHGGSLQHQQRSAEA 387 A A P+ +I+ + G S K + W LG L P+FHGG+L+ +AE Sbjct: 316 AQATAEMFPSLSISASFGQSGFTWPKATGAAGAVWGLGASLTQPLFHGGALRSMSEAAEQ 375 Query: 388 GLEQASAQYRSVVLGAFQNVADTLHAMAADADALKAAARAEDAAQRSFDAAQAQWKAGLA 447 A A YR VL AFQNVAD+L A++ DADA AAA A A+++ + AQ + G Sbjct: 376 AYLAAEANYRQTVLNAFQNVADSLVALSHDADAQTAAAAARQASEQGWRNAQRRVALGAV 435 Query: 448 GYPQVLLAQQNLLTASQSRVQAQAGRLADTVALFQALG 485 ++ L A S +A A RL T LFQA+G Sbjct: 436 PISAARSGERQYLNAHLSEARATASRLNATATLFQAMG 473 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 169 bits (429), Expect = 4e-43, Method: Compositional matrix adjust. Identities = 138/461 (29%), Positives = 220/461 (47%), Gaps = 17/461 (3%) Query: 30 LGGCAVGPDFKQPAAPQVSAYTAEHIEASQAIDGQRFVAGQDVAGQWWKAFGSPVLDSLV 89 LGGC++ P +++P AP S+Y A+ ++ Q V W + F L L+ Sbjct: 25 LGGCSLAPTYERPQAPVESSYPADAAGRAEL---------QAVNLGWRQFFPDQRLQQLI 75 Query: 90 DDALAHNADLQAAQAALRAARETAASQSGSLLPSVDLHFMPTRQKVADT---LASPLADN 146 AL +N DL+ A + AR LLP+++L R + A + P N Sbjct: 76 AAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAARTRTAASQSLTGQPTISN 135 Query: 147 SYIYNLHTAQLNIAYSPDIFGGTRRQIEAGRAQAEVQRFQRNATYLTLISNVVLAVVNEA 206 SY L AY D FG R +A A + + ++L++ V A + E Sbjct: 136 SYQVGLAVP----AYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLVAQVAQAYLAER 191 Query: 207 ALREQLQAAEKAEALATQQLELVRQQRALGALGLADVAAQESLLAQAQAVLPPLRKQLAQ 266 + EQL+ A++ L+L +++ +GA D ++L+ A+ L +Q AQ Sbjct: 192 SYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLVESARVAKTQLERQRAQ 251 Query: 267 QRDQIAVLVGRFPGQGGTPTLALSALKLPSELPLSLPSTLVQQRPDVRAAQAQWQAASAQ 326 + + +LVG+ G+ P LS K+ +++P LPS L+ RPD+R+A+ AA+A Sbjct: 252 AENALTLLVGKKIGEL-PPAQLLSDEKIVTDIPAGLPSDLLTNRPDIRSAEQSLLAANAN 310 Query: 327 VGVAIANRLPAFNITGTIGSSAQDISKLFTSGPGFWTLGLDLATPIFHGGSLQHQQRSAE 386 +G A A P ++TG GS++ + LF SG G W+ G L+ PIF G AE Sbjct: 311 IGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLPIFDFGRNSANLDLAE 370 Query: 387 AGLEQASAQYRSVVLGAFQNVADTLHAMAADADALKAAARAEDAAQRSFDAAQAQWKAGL 446 +A Y + AF+ VAD L A + + +KA A Q ++K G+ Sbjct: 371 VRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFLAAQQERLKLTDLRFKNGI 430 Query: 447 AGYPQVLLAQQNLLTASQSRVQAQAGRLADTVALFQALGGG 487 A L AQ++L +A Q+ +QA+ RL + + L++ALGGG Sbjct: 431 ASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGG 471 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 166 bits (421), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 147/463 (31%), Positives = 222/463 (47%), Gaps = 17/463 (3%) Query: 32 GCAVGPDFKQPAAPQVSAYTAEHIEASQAIDGQRFVAGQDVAGQWWKAFGSPVLDSLVDD 91 GCA GPD+ +P +A+T+E S A G WW+ FG L++L Sbjct: 18 GCASGPDYTRPQVDMPAAWTSEAPWRSMQ------PADATPRGPWWERFGDAQLNALQQQ 71 Query: 92 ALAHNADLQAAQAALRAARETAASQSGSLLPSVDLHFMPTRQKVADTLASPLADNSYIYN 151 ALA N L A A L AR + P V+L+ R +++ PL N N Sbjct: 72 ALAGNQTLAIATARLAQARAQLNVTGAAQTPQVNLNARAARSRISAN--RPLT-NYNSPN 128 Query: 152 LHTAQLN------IAYSPDIFGGTRRQIEAGRAQAEVQRFQRNATYLTLISNVVLAVVNE 205 T Q + ++Y D FG +R +EA A AE T L L + + N Sbjct: 129 FSTVQNDFALGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAELATNYFNL 188 Query: 206 AALREQLQAAEKAEALATQQLELVRQQRALGALGLADVAAQESLLAQAQAVLPPLRKQLA 265 L +L +A AL Q LEL + LGA D+A Q++LL + L +Q A Sbjct: 189 RELDVELDVVRRAIALQRQALELATARHDLGATSGLDMAQQQALLDSTLTQIDLLGRQRA 248 Query: 266 QQRDQIAVLVGRFPGQGGTPTLALSALKLPSELPLSLPSTLVQQRPDVRAAQAQWQAASA 325 Q IA L G P + T +L+ + P +P+ +PS ++++RPD+ AA+ AA+A Sbjct: 249 QYEHAIATLTGT-PAPAFSITPSLTPIT-PPAIPVGVPSDVLERRPDIAAAERAMAAANA 306 Query: 326 QVGVAIANRLPAFNITGTIGSSAQDISKLFTSGPGFWTLGLDLATPIFHGGSLQHQQRSA 385 Q+GVA A P+F + + G +++ + LF + W+LG+ + +F GG L+ + Sbjct: 307 QIGVASAAYYPSFMLQPSYGVDSRNWATLFNAPSLLWSLGVSASQSLFDGGRLRAGVDFS 366 Query: 386 EAGLEQASAQYRSVVLGAFQNVADTLHAMAADADALKAAARAEDAAQRSFDAAQAQWKAG 445 +AG E A YR VL A Q V D + +AA A + A +A+R + A ++++ G Sbjct: 367 QAGYEATVASYRRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVLEIASSRYEGG 426 Query: 446 LAGYPQVLLAQQNLLTASQSRVQAQAGRLADTVALFQALGGGW 488 Y V+ AQQ+LL + + Q RL +V L +ALGG W Sbjct: 427 ATPYLDVITAQQSLLNSERQAAQLMGQRLLVSVFLIKALGGDW 469 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 161 bits (408), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 149/478 (31%), Positives = 228/478 (47%), Gaps = 35/478 (7%) Query: 32 GCAVGPDFKQPAAPQVSAYTAEHIEASQAIDGQRFVAGQ---DVA-GQWWKAFGSPVLDS 87 GC+V P +++P+ +A+ +G ++ Q D+A G+WWK FG L++ Sbjct: 30 GCSVAPTYERPSVDTPAAFKEAAPATQTGENGSQWKTAQPAEDIARGEWWKIFGDEKLNA 89 Query: 88 LVDDALAHNADLQAAQAALRAARETAASQSGSLLPSVDLHFMPTRQKVADTLASPLAD-N 146 L D A N DL+AA A L AR P VD TRQ+ + AD + Sbjct: 90 LEDTAQQANQDLKAAAARLGQARALTRDARAGYFPQVDAGVGATRQRPSPASQGLPADAD 149 Query: 147 SYIYNLHTAQLNIAYSPDIFGGTRRQIEAGRAQAEVQRFQRNATYLTLISNVVLAVVNEA 206 + L+ AQ+N++Y D+FG ++A A A+ QR + L +V+LA+ + Sbjct: 150 TRPNTLYRAQVNVSYEVDLFGRVASTVDA--ATADAQRSE------ALFHSVLLALQADV 201 Query: 207 A----LREQLQAA----EKAEALATQQLELVRQQRALGALGLADVAAQESLLAQAQAVLP 258 A L +L AA L + L LV+++ G + DVA + LA AQ+ Sbjct: 202 AQQYFLVRELDAAAALYRGTVDLRQESLRLVQRRFDEGDISEVDVARARAELASAQSEAL 261 Query: 259 PLRKQLAQQRDQIAVLVGRFPGQGGTPTLALSALKLPSELPLSLPSTLVQQRPDVRAAQA 318 + +Q A +A+L+GR P + P L+ +L +P LPS L+++RPD+ AA+ Sbjct: 262 GIARQRATAEHALAILLGRTPAEFSLPPQPLT--RLAVTVPPGLPSALLERRPDIAAAER 319 Query: 319 QWQAASAQVGVAIANRLPAFNITGTIGSSAQDISKLFTSGPGFWTLG----LDLATPIFH 374 AA+A+VGVA + P+ N+TG +G + + LF + LG L+ P+F Sbjct: 320 AMAAANARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSSRTFLLGPLVGTALSMPLFD 379 Query: 375 GGSLQHQQRSAEAGLEQASAQYRSVVLGAFQNVADTLHAMAADADALKAAARAEDAAQRS 434 GG Q A A E+ A YR VL AF+ V D L A+ L RA+D A + Sbjct: 380 GGRRQAGVDRARASYEEQVASYRGTVLNAFREVEDNL----ANLRILDQQNRAQDEAVAA 435 Query: 435 FDA----AQAQWKAGLAGYPQVLLAQQNLLTASQSRVQAQAGRLADTVALFQALGGGW 488 + Q++ G Y V+ A + +L ++ V R TV L +ALGGGW Sbjct: 436 SARAAKLSHTQYREGAVSYFSVIDADRTVLQQQRASVTLDGERARSTVNLIRALGGGW 493 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.129 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,133,160 Number of extensions: 37974 Number of successful extensions: 275 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 221 Number of HSP's successfully gapped: 18 Length of query: 493 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 404 Effective length of database: 1,198,455 Effective search space: 484175820 Effective search space used: 484175820 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)