BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2177.p (413 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311754|ref|YP_003775846.1| Bcr/CflA family drug resistance... 718 0.0 gi|300313991|ref|YP_003778083.1| major facilitator superfamily p... 117 2e-27 gi|300312966|ref|YP_003777058.1| major facilitator superfamily p... 73 4e-14 gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 68 1e-12 gi|300311040|ref|YP_003775132.1| major facilitator superfamily p... 67 2e-12 >gi|300311754|ref|YP_003775846.1| Bcr/CflA family drug resistance transporter protein [Herbaspirillum seropedicae SmR1] Length = 417 Score = 718 bits (1853), Expect = 0.0, Method: Compositional matrix adjust. Identities = 357/395 (90%), Positives = 373/395 (94%) Query: 6 KHPPNHPRFLLFLICAFASAGQLAIDLYVPALPKMAIDYGTSAQAIQISVTAYLIAYAFG 65 +HPPNHPRFLLFLICAFASAGQLAIDLYVPALP+MAIDY TSAQAIQ SVT YLIAYAFG Sbjct: 13 QHPPNHPRFLLFLICAFASAGQLAIDLYVPALPEMAIDYATSAQAIQSSVTGYLIAYAFG 72 Query: 66 QLVFGPLADAYGRKRVLMLGLGLFTLGCVLSLAAPNLETFVAARVLQGFGIAATNLLAKA 125 QLVFGPLADAYGRKR+L LGLGLF+LGCVLSLAAPNLETFVAARVLQGFGIAATNLLAKA Sbjct: 73 QLVFGPLADAYGRKRILALGLGLFSLGCVLSLAAPNLETFVAARVLQGFGIAATNLLAKA 132 Query: 126 IITDSFSGQTLIHAYTYMATAWGLAPIVAPVIGAHLQEAFGWRSCLVFLLVYSLGMWVIL 185 IITDSF+GQ L+HAYTYMA AWGLAPI+APVIGAHLQEAFGWRSCLVFLLVYSLGMW +L Sbjct: 133 IITDSFAGQALVHAYTYMAIAWGLAPIIAPVIGAHLQEAFGWRSCLVFLLVYSLGMWAVL 192 Query: 186 WRYRETLAQPVHLHPGTLFKNARLVLASPVFQSCFLAQGLCYSILLVFNIVGPFMVQNTL 245 WRYRETLAQPV LHP TL +NAR+VLASPVFQSCFLAQGLCYSILLVFNIVGPFMVQNTL Sbjct: 193 WRYRETLAQPVRLHPATLARNARMVLASPVFQSCFLAQGLCYSILLVFNIVGPFMVQNTL 252 Query: 246 QQPPTFFGYLALAIGMMYFLGGISNRLHHPRLPTAEQRLRIGARVMTLAAFVMLALALGL 305 QPPTFFGYLALAIGMMYFLGGISNR+ H RLPTAEQRLR+GARVMT AAFVML LAL + Sbjct: 253 HQPPTFFGYLALAIGMMYFLGGISNRITHRRLPTAEQRLRLGARVMTGAAFVMLLLALSV 312 Query: 306 GLRVWTLIVPVLVMAFCAGAMYPTLMAKGNSIFPHIAGLTSAILGFALLLVSSAMMGLAG 365 GL VWTL PVLVMAFCAGAMYPTLMAKGNSIFPHIAGLTSAILGFALLLVS+AMMGLAG Sbjct: 313 GLTVWTLCAPVLVMAFCAGAMYPTLMAKGNSIFPHIAGLTSAILGFALLLVSAAMMGLAG 372 Query: 366 FVSIHSLTPLAVFFCIVALTVVAMVGKLLHHLQVP 400 FVSIHSLTPLA FFC+V LTV+ MVGKLLHHLQVP Sbjct: 373 FVSIHSLTPLAGFFCVVGLTVIVMVGKLLHHLQVP 407 >gi|300313991|ref|YP_003778083.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 406 Score = 117 bits (292), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 78/254 (30%), Positives = 134/254 (52%), Gaps = 9/254 (3%) Query: 16 LFLICAFASAGQLAIDLYVPALPKMAIDYGTSAQAIQISVTAYLIAYAFGQLVFGPLADA 75 L ++ + G LAID+Y+P+ +A D G +Q+++ +LI A GQ+ +GPL+D Sbjct: 17 LAVLAGLTAMGALAIDMYLPSFTAIAQDLGVGTNQVQLTLATFLIGVAVGQMFYGPLSDR 76 Query: 76 YGRKRVLMLGLGLFTLGCVLSLAAPNLETFVAARVLQGFGIAATNLLAKAIITDSFSGQT 135 +GRK L G+ L+ + +L + A N+ET + R+ QG G +A ++ +A++ D + Sbjct: 77 FGRKPPLFFGIALYFVCSLLCIFAQNIETLIVLRLFQGLGGSAGMVIPRAMVRDRMGAEG 136 Query: 136 LIHAYTYMATAWGLAPIVAPVIGAHLQEAFGWRSCLVFLLVYSLGMWVILWRY-RETL-- 192 A++ + +GLAPI+AP IG + WR V L ++ L + +Y ET+ Sbjct: 137 SAKAFSMLMLVFGLAPILAPFIGGLMLVFASWRGIFVVLTLFGLLCILGTRKYLTETVDV 196 Query: 193 --AQPVHLHPGTLFKNARLVLASPVFQSCFLAQGLCYSILLVFNIVGPFMVQNTLQQPPT 250 A+P+HL G + +L F S L GL + + + GPF+V P Sbjct: 197 SRAEPLHL--GRTLRQYWGLLRHRQFMSYVLCGGLVQAGMFAYIAGGPFVVIELHGVKPQ 254 Query: 251 FFGYL--ALAIGMM 262 +FG++ + AIG++ Sbjct: 255 YFGFVFASNAIGLI 268 >gi|300312966|ref|YP_003777058.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 544 Score = 73.2 bits (178), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 57/172 (33%), Positives = 81/172 (47%), Gaps = 17/172 (9%) Query: 37 LPKMAIDYGTSAQAIQISVTAYLIAYAFGQLVFGPLADAYGRKRVLMLGLGLFTLGCVLS 96 LP +A D G S I+ V++Y++ +A L G AD YGR+RVL+ G+ LF L + Sbjct: 59 LPTVARDLGASFSQIEWVVSSYVLCFAALLLPAGATADRYGRRRVLLGGIALFALTSLAC 118 Query: 97 LAAPNLETFVAARVLQGFGIAATNLLAKAIITDSFSGQTLIHAYTYMATAW-------GL 149 AP+ + AR LQG G A A A+I +F H AW GL Sbjct: 119 ALAPSAQALYGARALQGVGAAFLLAPALAVIGHAF------HDEDQRERAWALWGGIMGL 172 Query: 150 APIVAPVIGAHLQEAFGWRSCLVFLLVYSLGMWVILWRY----RETLAQPVH 197 ++AP+IG + GWR L L + V +WR+ R L +P+ Sbjct: 173 TMVLAPLIGGVVNTLLGWRWAFAVNLPACLLLAVAVWRHVPESRNPLPRPLD 224 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 68.2 bits (165), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 69/272 (25%), Positives = 113/272 (41%), Gaps = 19/272 (6%) Query: 13 RFLLFLICAFASAGQLAIDLYVPALPKMAIDYGTSAQAIQISVTAYLIAYAFGQLVFGPL 72 R L +++ A L + ALP+MA +G + + +TAY++ A + G + Sbjct: 30 RMLPYIVAATFFMEYLDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIPISGWV 89 Query: 73 ADAYGRKRVLMLGLGLFTLGCVLSLAAPNLETFVAARVLQGFGIAATNLLAKAIITDSFS 132 AD +G + V + + +FTL +L + N+ F AARVLQG G A + + I+ S Sbjct: 90 ADRFGSRTVFGMAIAIFTLASILCGFSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRSTG 149 Query: 133 GQTLIHAYTYMATAWGLAPIVAPVIGAHLQEAFGWRSCLVFLLVYSLGMWVILWRYRETL 192 L+ A + +AP+V P IG F+ Y+ W+ L L Sbjct: 150 KDRLMKAIATITWPAIVAPVVGPSIGG-------------FITTYASWHWIFLMNVPFGL 196 Query: 193 AQPVHLHPGTLFKNARLVLASPVFQSCFLAQGLCYSILLVFNIVGPFMVQNTLQQPPTFF 252 A + + +N V P+ FL G + LL + M N Sbjct: 197 AAFTAVM--MVVRNEHGVSKRPLDLVGFLLSGAALTSLLYGTEIASHMESNI----KVAL 250 Query: 253 GYLALAIGMMYFLGGISNRLHHPRLPTAEQRL 284 G++AL + + Y R+ HP L + R+ Sbjct: 251 GFVALGVVLGYGAWRHMQRIEHPLLDFSNLRI 282 >gi|300311040|ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 67.4 bits (163), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 42/133 (31%), Positives = 68/133 (51%) Query: 36 ALPKMAIDYGTSAQAIQISVTAYLIAYAFGQLVFGPLADAYGRKRVLMLGLGLFTLGCVL 95 +LP +A D +++++TAY+ A + G +AD G ++V + +F G +L Sbjct: 9 SLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMIVFMTGSIL 68 Query: 96 SLAAPNLETFVAARVLQGFGIAATNLLAKAIITDSFSGQTLIHAYTYMATAWGLAPIVAP 155 + +L TFVAAR LQG G A + + II S L+ A +Y+ L P+V P Sbjct: 69 CAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPALLGPVVGP 128 Query: 156 VIGAHLQEAFGWR 168 ++G + F WR Sbjct: 129 LLGGFITTYFHWR 141 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.142 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,300,811 Number of extensions: 55173 Number of successful extensions: 362 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 361 Number of HSP's successfully gapped: 17 Length of query: 413 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 325 Effective length of database: 1,203,190 Effective search space: 391036750 Effective search space used: 391036750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 132 (55.5 bits)