BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2164.p (450 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311765|ref|YP_003775857.1| adenosylmethionine-8-amino-7-ox... 769 0.0 gi|300312816|ref|YP_003776908.1| aminotransferase class III prot... 185 8e-48 gi|300310456|ref|YP_003774548.1| family II aminotransferase [Her... 183 3e-47 gi|300313929|ref|YP_003778021.1| omega-amino acid/pyruvate amino... 177 1e-45 gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferas... 147 2e-36 >gi|300311765|ref|YP_003775857.1| adenosylmethionine-8-amino-7-oxononanoate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 448 Score = 769 bits (1986), Expect = 0.0, Method: Compositional matrix adjust. Identities = 376/448 (83%), Positives = 407/448 (90%), Gaps = 1/448 (0%) Query: 1 MTLSRPDSLAARSLRSVWHPCTQMRRHESMPPIALARAHGPWLVDTEGRRYLDAISSWWV 60 M+ S +L ARSLRSVWHPCTQMRRHES PPIA+ RAHG WL D++GRRYLDAISSWWV Sbjct: 1 MSGSASSALVARSLRSVWHPCTQMRRHESAPPIAITRAHGLWLHDSDGRRYLDAISSWWV 60 Query: 61 NLFGHTNARINAALVDQLGQLEHAMLAGFTHEPVVQLSERLAALTGQALGHCFYASDGAS 120 NLFGHTN RINAALV+QLGQLEHAMLAGFTHEPVV LSE+LAALTGQALGHCFYASDGAS Sbjct: 61 NLFGHTNTRINAALVEQLGQLEHAMLAGFTHEPVVALSEQLAALTGQALGHCFYASDGAS 120 Query: 121 AVEIALKMSVHAWRNAGQADKNEFICIAGGYHGETVGALAVTDTPLFRTAYEALLRPAHI 180 AVEIALKMSVHAWRN GQ DKNEFICIAGGYHGETVGAL VTDTPLFR+AYE+LLRPAH+ Sbjct: 121 AVEIALKMSVHAWRNGGQPDKNEFICIAGGYHGETVGALGVTDTPLFRSAYESLLRPAHV 180 Query: 181 AASPDARQARPGESSADVAARALADVERLLQQRQGRIAAVIVEPLVQCAAGMAMHDASYV 240 AASPDARQA PGES+ +VAARAL DVER+LQQRQGRIAA+IVEPLVQCAAGMAMHD YV Sbjct: 181 AASPDARQAAPGESAEEVAARALDDVERILQQRQGRIAALIVEPLVQCAAGMAMHDPGYV 240 Query: 241 RGLRRLCDTYGAHLIADEIAVGCGRTGSFFACEQAGIWPDLLCLSKGISGGYLPLSLVMA 300 RGLR LCD YGAHLIADEIAVGCGRTGSFFACEQAG+WPDLLCLSKGISGGYLPLSLVM+ Sbjct: 241 RGLRSLCDRYGAHLIADEIAVGCGRTGSFFACEQAGVWPDLLCLSKGISGGYLPLSLVMS 300 Query: 301 SAAIYQAFYDDDLRRGFLHSHSYTGNPLACRAALETLAIFEQEQVLARNGRFAPVLAQAL 360 S AIYQAFYDDD+RRGFLHSHSYTGNPLACRAAL TLAIF ++ VL RN FAPVLA AL Sbjct: 301 SEAIYQAFYDDDVRRGFLHSHSYTGNPLACRAALATLAIFAEDAVLERNRAFAPVLAAAL 360 Query: 361 QPLAQDGRVRHVRQRGMIWACDAVIEDAATA-SAFARRLAAEAAQRELLVRPIGAALYVM 419 QPLAQD RVRHVRQRGMIWA DAV+E +A A +AF+RR+AAEAAQRE+LVRPIGA+LY+M Sbjct: 361 QPLAQDPRVRHVRQRGMIWAYDAVLEGSAAATTAFSRRMAAEAAQREVLVRPIGASLYLM 420 Query: 420 PPYILDAEQAQWLGERLYAAFDAAFAAV 447 PPY+LD EQA WLG +L+AAF A A+ Sbjct: 421 PPYLLDEEQAHWLGRQLHAAFAATMEAL 448 >gi|300312816|ref|YP_003776908.1| aminotransferase class III protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 185 bits (469), Expect = 8e-48, Method: Compositional matrix adjust. Identities = 139/440 (31%), Positives = 216/440 (49%), Gaps = 25/440 (5%) Query: 25 RRHESMPPIALARAHGPWLVDTEGRRYLDAISSWWVNLFGHTNARINAALVDQLGQLEHA 84 R S P+A+A G L+D +G+RY+DA V+ GH + R+ A+ Q+G+L +A Sbjct: 7 RNPRSKLPMAVA-GEGMELIDQDGKRYIDASGGAAVSCLGHGHPRVIEAIRKQVGELAYA 65 Query: 85 MLAGFTHEPVVQLSERLAALTGQALGHCFYASDGASAVEIALKMSVHAWRNAGQADKNEF 144 + FT P +L+ LA +L H ++ S G+ AVE ALK++ + GQ + Sbjct: 66 HTSFFTTAPAEELAAMLADAAPGSLNHVYFLSGGSEAVEAALKLARQYYVEVGQPQRRHI 125 Query: 145 ICIAGGYHGETVGALAVTDTPLFRTAYEALLRPAHIAASPDARQAR-PGESSADVAARAL 203 I YHG T+GALA+ R + +L AH + A + R GES R Sbjct: 126 IARRQSYHGNTLGALAIGGNAWRREMFMPMLIEAHHVSPCYAYRNRADGESDVQYVQRLA 185 Query: 204 ADVE-RLLQQRQGRIAAVIVEPLVQCAAGMAMHDASYVRGLRRLCDTYGAHLIADEIAVG 262 ++E ++L ++ A + E +V AG Y R +R +CD YG LI DE+ G Sbjct: 186 DELEQKILSLGADQVIAFVAETVVGATAGAVPPVGDYFRKIRAVCDKYGVLLILDEVMSG 245 Query: 263 CGRTGSFFACEQAGIWPDLLCLSKGISGGYLPLSLVMASAAIYQAFYDDDLRRG---FLH 319 GRTG FACE+ G+ PD++ ++KG+ GY P+ ++ S IY D + RG F H Sbjct: 246 MGRTGYLFACEEDGVVPDIVVIAKGLGAGYQPIGAMICSDHIY-----DAVLRGSGFFQH 300 Query: 320 SHSYTGNPLACRAALETLAIFEQEQVLAR-NGRFAPVLAQALQPLAQDGRVRHVRQRGMI 378 H+Y G+ AC AA+ ++E++L R + ++ Q V +R RG+ Sbjct: 301 GHTYIGHATACAAAVAVQKTIQEERLLENVRQRGEQLRSELRQAFGDQAHVGDIRGRGLF 360 Query: 379 WACDAVIEDAAT--ASAFAR---RLAAEAAQRELLVRPIGAA--------LYVMPPYILD 425 + V E ++ S R R+ AEA +R LLV P+G + + PP+I Sbjct: 361 VGVELVAERSSKLPLSPDLRTHARVKAEAMKRGLLVYPMGGTIDGKNGDHILLAPPFIAS 420 Query: 426 AEQAQWLGERLYAAFDAAFA 445 + + +RL A A+ A Sbjct: 421 SNDISEIVQRLSDAVAASVA 440 >gi|300310456|ref|YP_003774548.1| family II aminotransferase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 183 bits (464), Expect = 3e-47, Method: Compositional matrix adjust. Identities = 112/395 (28%), Positives = 192/395 (48%), Gaps = 8/395 (2%) Query: 8 SLAARSLRSVWHPCTQMRRHESMPPIALARAHGPWLVDTEGRRYLDAISSWWVNLFGHTN 67 +LAA R + HP T +R HE + P+ L G +L D + ++ LDA S W G+ Sbjct: 5 ALAALDRRHLIHPVTSLREHEELGPLILKSGQGAYLTDHQDKQLLDAFSGLWCVNTGYGQ 64 Query: 68 ARINAALVDQLGQLEHAMLAGFTH---EPVVQLSERLAALTGQALGHCFYASDGASAVEI 124 + A +Q+ +L +A G+ H EP + L+++L ++ +L H ++ G+ AV+ Sbjct: 65 ESVIRAATEQMQRLPYA--TGYFHFASEPAILLAKKLVDISPASLQHVYFTLGGSDAVDA 122 Query: 125 ALKMSVHAWRNAGQADKNEFICIAGGYHGETVGALAVTDTPLFRTAYEALLRPAHIAASP 184 A++ H + G+ K FI + GYHG + +T P F + L H SP Sbjct: 123 AIRYITHYHHSIGKPGKQHFISLQRGYHGSSSVGAGLTALPNFHYHFNLPLPTQHYLPSP 182 Query: 185 DARQARPGESSADVAARALADVERLLQQRQGRIAAVIVEPLVQCAAGMAMHDASYVRGLR 244 ++ A +AA A +++ + +AA EP +Q + G+ + +++ ++ Sbjct: 183 YPYRSELQGDEAIIAASVQALRDKVAELGADNVAAFFCEP-IQGSGGVVVPPKGWLKAMQ 241 Query: 245 RLCDTYGAHLIADEIAVGCGRTGSFFACEQAGIWPDLLCLSKGISGGYLPLSLVMASAAI 304 C+ + DE+ G GRTG FAC + PDL+ ++KG++ GY P+ VM S + Sbjct: 242 LACNELDILFVVDEVITGFGRTGPMFACLDEDVQPDLMTMAKGLTAGYAPMGAVMMSDRV 301 Query: 305 YQAFYDD-DLRRGFLHSHSYTGNPLACRAALETLAIFEQEQVLARNGRFAPVLAQALQPL 363 Y D + H +Y+ +P++ ALE L ++E+ +L R PV A L+ L Sbjct: 302 YNGIADGAPMSTVVGHGQTYSAHPVSAAVALEVLRLYEEGGLLDNGRRLEPVFAGGLRAL 361 Query: 364 AQDGRVRHVRQRGMIWACDAVIEDAATASAFARRL 398 V R RG++ A + ++ D T + FA L Sbjct: 362 LAHPLVGDARSRGLLGALE-LVADKQTKARFAPEL 395 >gi|300313929|ref|YP_003778021.1| omega-amino acid/pyruvate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 442 Score = 177 bits (449), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 130/440 (29%), Positives = 203/440 (46%), Gaps = 8/440 (1%) Query: 3 LSRPDSLAARSLRSVWHPCTQMRRHESMPPIALARAHGPWLVDTEGRRYLDAISSWWVNL 62 +S D SL + W P T R ++ P + L A G D G LD + W Sbjct: 1 MSSQDPTHPSSLSAYWMPFTANRNFKAAPRM-LVSAEGMHYRDDAGNTILDGTAGLWCVP 59 Query: 63 FGHTNARINAALVDQLGQLEHAMLAGFTHEPVVQLSERLAALTGQALGHCFYASDGASAV 122 GH + +I +A+ + L++A H +L+ERL A TG G+ FY G+ AV Sbjct: 60 LGHAHPKIVSAVQKSVATLDYAPSFQLGHPSAFELAERLKAYTGNRFGNVFYTGSGSEAV 119 Query: 123 EIALKMSVHAWRNAGQADKNEFICIAGGYHGETVGALAVTDTPLFRTAYEALLRPA--HI 180 + ALK+++ R G A + I GYHG G ++V R + L P H+ Sbjct: 120 DTALKIALAYHRAKGNATRTRLIGRERGYHGVGFGGMSVGGIGGNRKTFSTALLPGVDHL 179 Query: 181 AASPDARQARPGESSADVAARALADVERLLQ-QRQGRIAAVIVEPLVQCAAGMAMHDASY 239 + + + + + A ++ERL+ IAAVIVEP V + G+ + Y Sbjct: 180 PHTHNLEKNAFTKGEPEYGAHLADELERLVTLHDASNIAAVIVEP-VAGSTGVLIPPKGY 238 Query: 240 VRGLRRLCDTYGAHLIADEIAVGCGRTGSFFACEQAGIWPDLLCLSKGISGGYLPLSLVM 299 ++ LR +C +G LI DE+ G GR G+ FA + + PD+ +KG++ G +P+ V Sbjct: 239 LKRLREICTKHGILLIFDEVITGFGRLGTPFASDYFDVEPDIFTTAKGLTNGVVPMGAVF 298 Query: 300 ASAAIYQAFYDDDLRRGFLHSHSYTGNPLACRAALETLAIFEQEQVLARNGRFAPVLAQA 359 I+ AF + H ++Y+G+PLAC A L +L +F+ E VL A +A Sbjct: 299 VKPEIHDAFMNGPDGIELFHGYTYSGHPLACAAGLASLEVFQTEGVLENAQAVADYFQEA 358 Query: 360 LQPLAQDGRVRHVRQRGMIWACD-AVIEDAATASAFARRLAAEAAQRELLVRPIGAALYV 418 L V VR G+I + A I A A+ + A +L+R G + + Sbjct: 359 AHALRGLPHVIDVRTIGLIAGIELASIPGRVGARAYDAFV--RAFNDGILIRVTGDIIAL 416 Query: 419 MPPYILDAEQAQWLGERLYA 438 PP I++ +Q L +L A Sbjct: 417 SPPLIINKQQIDELFGKLAA 436 >gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 426 Score = 147 bits (371), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 130/420 (30%), Positives = 194/420 (46%), Gaps = 57/420 (13%) Query: 37 RAHGPWLVDTEGRRYLDAISSWWVNLFGHTNARINAALVDQLGQLEHAMLAGFTHEPVVQ 96 RA L D EGRR++D + V GH + ++ A+ Q+ + H + V+ Sbjct: 32 RAANAELWDVEGRRFIDFAAGIAVLNTGHRHPKLLDAMRAQMDKFTHTAYQIVPYASYVE 91 Query: 97 LSERLAALT-GQALGHCFYASDGASAVEIALKMS-VHAWRNAGQADKNEFICIAGGYHGE 154 L+ER+ LT G + S GA AVE A+K++ H R I AGG+HG Sbjct: 92 LAERINRLTPGNYPKKTAFFSTGAEAVENAIKIARAHTGRPG-------VIAFAGGFHGR 144 Query: 155 TVGALAVTDT-------------PLFRTAYEALLRPAHIAASPDARQARPGESSADVAAR 201 T+ +A+T +F Y + L H S DA +A G +D+ A+ Sbjct: 145 TMMGMALTGKVAPYKLGFGPFPGDVFHAPYPSAL---HGITSEDALEAVKGLFKSDIEAK 201 Query: 202 ALADVERLLQQRQGRIAAVIVEPLVQCAAGMAMHDASYVRGLRRLCDTYGAHLIADEIAV 261 R+AA+I+EP VQ G A ++RGLR LCD +G LIADE+ Sbjct: 202 --------------RVAAIILEP-VQGEGGFYAAPADFMRGLRALCDEHGILLIADEVQS 246 Query: 262 GCGRTGSFFACEQAGIWPDLLCLSKGISGGYLPLSLVMASAAIYQAFYDDDLRRGFLHSH 321 G GRTG FA E + PDL+ ++K ++GG +PLS V A I A L Sbjct: 247 GYGRTGKLFAMEHYDVLPDLMTMAKSLAGG-MPLSAVNGRAEIMDAPAPGGL------GG 299 Query: 322 SYTGNPLACRAALETLAIFEQEQVLARNGRFAPVLAQALQPL-AQDGRVRHVRQRGMIWA 380 +Y GNPLA +AL L + E+EQ++ R R L + L+ L + ++ VR G + A Sbjct: 300 TYAGNPLAIASALAVLDVMEEEQLVTRGQRLGDKLQEHLKELRSSVPQIAEVRGVGAMVA 359 Query: 381 CDAVIEDAATA---SAFARRLAAEAAQRELLVRPIGA----ALYVMPPYILDAEQAQWLG 433 + D AT + + +++ A LL+ G+ ++ P I D + LG Sbjct: 360 VE--FADPATGKPDAEYTKKVQQHALNNGLLLLTCGSYGNVIRFLFPLTIPDTVMDEALG 417 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.134 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,372,425 Number of extensions: 56340 Number of successful extensions: 302 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 285 Number of HSP's successfully gapped: 10 Length of query: 450 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 362 Effective length of database: 1,203,190 Effective search space: 435554780 Effective search space used: 435554780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)