BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2150.p (447 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311780|ref|YP_003775872.1| major facilitator superfamily p... 813 0.0 gi|300313056|ref|YP_003777148.1| major facilitator superfamily p... 149 6e-37 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 140 2e-34 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 122 5e-29 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 110 2e-25 >gi|300311780|ref|YP_003775872.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 447 Score = 813 bits (2101), Expect = 0.0, Method: Compositional matrix adjust. Identities = 417/447 (93%), Positives = 435/447 (97%) Query: 1 MASTSIHDGQGSGQFRQQSEHPSTSHEHGAIEPGEIAVGVVIGRASEYFDFFVYALASVL 60 MASTSIH+GQGSG+ S HPSTSH+HG IEPGEIAVGVVIGRASEYFDFFVYALASVL Sbjct: 1 MASTSIHNGQGSGELGPHSTHPSTSHDHGKIEPGEIAVGVVIGRASEYFDFFVYALASVL 60 Query: 61 VFPMLFFPNAPRLEGTLYAFVIFSFAFLARPIGTTIFMAIQKHYGREVKLTLALFMLGFS 120 VFP +FFPN P+LEGTLYAFVIF+FAFLARPIGTTIFMAIQKHYGREVKLTLALFMLGFS Sbjct: 61 VFPAVFFPNEPKLEGTLYAFVIFAFAFLARPIGTTIFMAIQKHYGREVKLTLALFMLGFS 120 Query: 121 TAGIALLPTYDHIGVWAIILLSLLRVGQGLALGGAWDGLPSLLAMNAPQNKRGWYAALGQ 180 TAGIALLPT++HIGVWAIILLSLLRVGQGLALGGAWDGLPSLLAMNAPQ+KRGWYAALGQ Sbjct: 121 TAGIALLPTFEHIGVWAIILLSLLRVGQGLALGGAWDGLPSLLAMNAPQHKRGWYAALGQ 180 Query: 181 LGAPLGFIVAASLFAYMLSNLSSADFLEWGWRYPFYVAFAINVVALFSRLRLVSAPEYAK 240 LGAPLGF+VAASLFAYMLS+L++ADFLEWGWRYPFYVAFAINVVALFSRLRLVSAPEYAK Sbjct: 181 LGAPLGFVVAASLFAYMLSSLTTADFLEWGWRYPFYVAFAINVVALFSRLRLVSAPEYAK 240 Query: 241 LLDERELEPAGAVELVTTQGRNIIIGALAALASYALFHLVTVFPLSWVKLYSLREATDFL 300 LLDERELEPAG VEL TTQGRNIIIGALAALASYALFHLVTVFPLSW+KLYSLREATDFL Sbjct: 241 LLDERELEPAGVVELATTQGRNIIIGALAALASYALFHLVTVFPLSWIKLYSLREATDFL 300 Query: 301 VVQIWGGVIMAAGIVVSGLIADRFGRRNTLGTMALLIAILSAFVPTLMNGGESGQNTFIL 360 VVQIWGG+IMA GIV+SGLIADRFGRRNTLG+MALLIAILSAFVPTLMNGGE+GQNTFIL Sbjct: 301 VVQIWGGLIMAVGIVISGLIADRFGRRNTLGSMALLIAILSAFVPTLMNGGEAGQNTFIL 360 Query: 361 IGFALLGLSYGQAAGAVTANFKTRYRYTGAALTSDLAWLVGAGFAPLVALGLSAHFGMAY 420 IGFALLGLSYGQAAGAVTANFK RYRYTGAALTSDLAWLVGAGFAPLVALGLSAHFGMAY Sbjct: 361 IGFALLGLSYGQAAGAVTANFKARYRYTGAALTSDLAWLVGAGFAPLVALGLSAHFGMAY 420 Query: 421 VSFYLLSGAVGSLAALSLNRALEARDD 447 VSFYLLSGAVGSLAALSLNRALEARDD Sbjct: 421 VSFYLLSGAVGSLAALSLNRALEARDD 447 >gi|300313056|ref|YP_003777148.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 465 Score = 149 bits (375), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 97/323 (30%), Positives = 166/323 (51%), Gaps = 10/323 (3%) Query: 18 QSEHPSTSHEHGAIEPGEIAVGVVIGRASEYFDFFVYALASVLVFPMLFFPNAPRLEGTL 77 QS + H+ + A IG A EY+DFF+YA A+ L+FP LFFP + Sbjct: 2 QSTQSNAHHQQSK----KAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAII 57 Query: 78 YAFVIFSFAFLARPIGTTIFMAIQKHYGREVKLTLALFMLGFSTAGIALLPTYDHIGVWA 137 + + ++ARPIG + + +GR+ L +F++GFST G+ LLPTYD +G+WA Sbjct: 58 ASLATYGVGYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWA 117 Query: 138 IILLSLLRVGQGLALGGAWDGLPSLLAMNAPQNKRGWYAALGQLGAPLGFIVAASLFAYM 197 LL ++R+ QG A+ G G S++ ++P +RG++++ G G I+AA++F + Sbjct: 118 PALLVIMRLAQGFAVAGEISGASSMILEHSPFGRRGYFSSFTLQGVQAGQILAAAVFLPL 177 Query: 198 LSNLSSADFLEWGWRYPFYVAFAINVVALFSRLRLVSAPEYAKLLDERELE-PAGAVELV 256 + + F WGWR PF ++F + + R + P + K D+ EL+ + V+ Sbjct: 178 AHYMPAEQFNVWGWRIPFLLSFLVIIAGWVIRRTVDETPVF-KAADKEELKRRSPVVDAF 236 Query: 257 TTQGRNIIIGALAALASYALFHLVTVFPLSWV--KLYSLREATD-FLVVQIWGGVIMAAG 313 N++ AL + + + T+F ++ Y + A D +L + + G ++ Sbjct: 237 RDCSPNMLRVVCMALMN-VIPVVATIFGAAYAVQPAYGIGFAKDVYLWIPVVGNIVAVLV 295 Query: 314 IVVSGLIADRFGRRNTLGTMALL 336 I G ++DR GRR + +LL Sbjct: 296 IPYVGKLSDRIGRRPPIIVGSLL 318 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 140 bits (352), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 112/417 (26%), Positives = 193/417 (46%), Gaps = 11/417 (2%) Query: 38 VGVVIGRASEYFDFFVYALASVLVFPMLFFPNAPRLEGTLYAFVIFSFAFLARPIGTTIF 97 + ++G A E++DF VY S ++ LFFP L A F F RP+G + Sbjct: 12 IAAIVGNALEWYDFIVYGFFSAII-ARLFFPADNEYTSLLVALATFGIGFFMRPVGGVLL 70 Query: 98 MAIQKHYGREVKLTLALFMLGFSTAGIALLPTYDHIGVWAIILLSLLRVGQGLALGGAWD 157 GR+ + + + ++ + A IA PTY IG+ A I++ + R+ QG A GG + Sbjct: 71 GLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQGFATGGEYS 130 Query: 158 GLPSLLAMNAPQNKRGWYAALGQLGAPLGFIVAASLFAYMLSNLSSADFLEWGWRYPFYV 217 + L +AP +KRG Y + +G L A + A + NLS WGWR PF + Sbjct: 131 SSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALDSWGWRLPFVL 190 Query: 218 AFAINVVALFSRLRLVSAPEYAKLLDERELEPAGAVELVTTQGRNIIIGALAALASYALF 277 I V L+ R + ++ + ++ P +++V T R +++ + F Sbjct: 191 GLLIGPVGLWIRRHMHETDDFLESANKPNAAPIKLLDVVKTNLRGVLVSMGQVINGTVAF 250 Query: 278 HLVTV-FPLSWVKLYSLREATDFLVVQIWGGVIMAAGIVVSGLIADRFGRRNTL--GTMA 334 ++V V P K L F+ VQ+ ++ A I +G ++DR GRR L ++A Sbjct: 251 YVVLVNMPTFANKQLGLPLDQAFM-VQMIAVALLTAVIPFAGALSDRLGRRTVLLYSSLA 309 Query: 335 LLIAILSAFVPTLMNGGESGQNTFIL--IGFALLGLSYGQAAGAVTANFKTRYRYTGAAL 392 L+ + FV ++ S ++ + ++G ++G A++ F TR R TG A+ Sbjct: 310 FLLMVYPLFV--WVSAAPSLPRLLVMQVLLCIVIGANFGPMPTALSEQFPTRVRSTGLAV 367 Query: 393 TSDLAWLVGAGFAPLVALGLSAHFG--MAYVSFYLLSGAVGSLAALSLNRALEARDD 447 +LA ++ GFAP + L+ G +A + L + +G AA ++ A + Sbjct: 368 AYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLFAACIGVSAAWAMRDQSPAAKN 424 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 122 bits (307), Expect = 5e-29, Method: Compositional matrix adjust. Identities = 103/371 (27%), Positives = 173/371 (46%), Gaps = 8/371 (2%) Query: 38 VGVVIGRASEYFDFFVYALASVLVFPMLFFPNAPRLEGTLYAFVIFSFAFLARPIGTTIF 97 V VIG E+FDF VY+ +V++ LFFP L L A F F RP+G + Sbjct: 24 VATVIGNGLEWFDFTVYSFFAVII-AKLFFPTGNDLTSLLLAVATFGVGFFMRPVGGILL 82 Query: 98 MAIQKHYGREVKLTLALFMLGFSTAGIALLPTYDHIGVWAIILLSLLRVGQGLALGGAWD 157 GR+ L++ + ++ T I L PTY+ IG++A +++ + R+ QG + GG Sbjct: 83 GIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLLQGFSAGGEMG 142 Query: 158 GLPSLLAMNAPQNKRGWYAALGQLGAPLGFIVAASLFAYMLSNLSSADFLEWGWRYPFYV 217 + L AP+ +R +Y++ Q + ++ A++ ++ ++L +A WGWR PF + Sbjct: 143 SATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALASWGWRLPFLL 202 Query: 218 AFAINVVALFSRLRLVSAPEYAKLLDERELEPAGAVELVTTQGRNIIIG-ALAALASYAL 276 I V + R L P + L+E++ + E++ R ++ L + Sbjct: 203 GIIIGPVGYYIRHHLEETPTF---LEEKDKTDSPLKEVIRNFPRETSASFSMVILWTVCT 259 Query: 277 FHLVTVFPLSWVKLYSLREATDFLVVQIWGGVIMAAGIVVSGLIADRFGRRNTLGTMALL 336 + L+ P VK+ L + T F+ + G IM +V G +ADR GRR L A L Sbjct: 260 YVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIV-GRLADRIGRRPLLSGSAAL 318 Query: 337 IAILSAFVPTLMNGGESGQNTFI--LIGFALLGLSYGQAAGAVTANFKTRYRYTGAALTS 394 I +L+ + +N S I L+ L+ G A + F + TG ++ Sbjct: 319 ILMLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAKVLSTGLSVAY 378 Query: 395 DLAWLVGAGFA 405 +LA + GFA Sbjct: 379 NLAVTIFGGFA 389 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 110 bits (275), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 119/410 (29%), Positives = 192/410 (46%), Gaps = 14/410 (3%) Query: 42 IGRASEYFDFFVYALASVLVFPMLFFPNAPRLEGTLYAFVIFSFAFLARPIGTTIFMAIQ 101 IG A E+FD +Y A +V FFP L F F +F RP+G + A Sbjct: 26 IGNALEWFDILIYG-AFAVVIAKQFFPTGDDSVSLLLTFATFGVSFFMRPLGAVVLGAYS 84 Query: 102 KHYGREVKLTLALFMLGFSTAGIALLPTYDHIGVWAIILLSLLRVGQGLALGGAWDGLPS 161 GR+ L L++ ++ TA IA +P+Y IG+ A ++L ++ QG + GG + + Sbjct: 85 DRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGKMIQGFSAGGEFGSSTA 144 Query: 162 LLAMNAPQNKRGWYAALGQLGAPLGFIVAASLFAYMLSNLSSADFL-EWGWRYPFYVAFA 220 L +AP ++RG++++ + ++AA LF +L+N+ + + L WGWR PF Sbjct: 145 FLVEHAP-HRRGFFSSWQVASQGISLLLAA-LFGAVLNNMLTPEQLASWGWRVPFIFGLL 202 Query: 221 INVVALFSRLRLVSAPEYAKLLDERELEPAGAVELVTTQGRNIIIGALAALASYALFHLV 280 I ++ R L APE+ + E A + Q ++IGA + + + +L Sbjct: 203 IAPAGIYIRRHLDEAPEFK---ESSEKTNAPLRDTFAHQKMRLLIGAGSVIMATVSVYLS 259 Query: 281 TVFPLSWVKLYSLREATDFLVVQIWGGVIMAAGIVVSGLIADRFGRRNTLGTMALLIAIL 340 P VK L + F + + G+IM G + G ++D+ GR + T + L +L Sbjct: 260 LYIPTYAVKQLGLPAWSSFAAMSV-AGLIMFIGSPLVGALSDKIGRTPFMITSSALYIVL 318 Query: 341 S--AFVPTLMNGGESGQNTFILIGFALLGLSYGQAAGAVTAN-FKTRYRYTGAALTSDLA 397 + FV L N Q + +L Y A A+ A+ F R TG +L ++A Sbjct: 319 TYPMFV-FLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADIFPVATRGTGMSLAYNIA 377 Query: 398 WLVGAGFAPLVALGLSAHFGMAY-VSFYLLSGAVGSL-AALSLNRALEAR 445 + GFA L+ L G VS++++ GAV SL A+LS L R Sbjct: 378 VTIFGGFAGLIITWLIDFTGDKLSVSYFVIFGAVLSLIASLSARLVLRLR 427 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.140 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,438,137 Number of extensions: 64567 Number of successful extensions: 352 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 327 Number of HSP's successfully gapped: 11 Length of query: 447 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 359 Effective length of database: 1,203,190 Effective search space: 431945210 Effective search space used: 431945210 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)