BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0001.2143.p
         (441 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311782|ref|YP_003775874.1| chloride channel protein [Herba...   704   0.0  
gi|300311435|ref|YP_003775527.1| chloride channel protein [Herba...    67   4e-12

>gi|300311782|ref|YP_003775874.1| chloride channel protein
           [Herbaspirillum seropedicae SmR1]
          Length = 442

 Score =  704 bits (1817), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 371/436 (85%), Positives = 397/436 (91%), Gaps = 4/436 (0%)

Query: 1   MKHFKYPEQLYLLPYIAKWCLIACLIAVLAGSASAFFLFSLEYATQWREAHPWILWLLPL 60
           MK+  YPEQLYLLPYI KW LIACLIA LAGSASAFFLF+L++AT WRE H WI+WLLPL
Sbjct: 1   MKNHHYPEQLYLLPYIGKWTLIACLIAALAGSASAFFLFALDWATHWRERHAWIIWLLPL 60

Query: 61  GGFAVGWLYLHTGKPVEAGNNLLIDEIHDPKKVIPLRMVPLVLFGTVMSHLFGASVGREG 120
           GGFAVGWLYLHTGKPVEAGNNLLIDEIHDP+KVIPLRM PLVL GTV SHLFGASVGREG
Sbjct: 61  GGFAVGWLYLHTGKPVEAGNNLLIDEIHDPRKVIPLRMAPLVLLGTVASHLFGASVGREG 120

Query: 121 TAVQMGGALADQLTHVLRLRQEDRRLLLMAGISAGFASVFGTPMAGALFGLEVLAIGRMR 180
           TAVQMGGALADQLTH+LRLR EDRR+LLMAGISAGF+SVFGTPMAGA+FGLEVLAIGRMR
Sbjct: 121 TAVQMGGALADQLTHLLRLRPEDRRILLMAGISAGFSSVFGTPMAGAIFGLEVLAIGRMR 180

Query: 181 YDAILPCFVSAIVADQVGLAWGVHHTHYAMSGSAPIAWWSVGAVIVAGVVFGLVGMVFAN 240
           Y+AI PCFV+AI ADQVGLAWGVHHTHYAM+ SAP+A WSV AVI AGVVFGL GM+FAN
Sbjct: 181 YEAIFPCFVAAIAADQVGLAWGVHHTHYAMNASAPMALWSVAAVIAAGVVFGLAGMIFAN 240

Query: 241 STHRLSAFMKRHVSYAPLRPFIGGLVVALAVWAVGTHRYIGLGIPVIVEAFQHPLAPWDF 300
           STH LSAFMKRHVSYAPLRPFIGG +VALAVWAVGTHRYIGLGIPVIVEAFQ PLAPWDF
Sbjct: 241 STHTLSAFMKRHVSYAPLRPFIGGFIVALAVWAVGTHRYIGLGIPVIVEAFQQPLAPWDF 300

Query: 301 LGKMLFTVTSLGTGFKGGEVTPLFYIGATLGNAMAPLLHLPFSMLAGIGFVAVFAGAANT 360
           LGKM+FTVTSLG+GFKGGEVTPLFYIGATLGNA+APLLHLPF +LAGIGFVAVFAGAANT
Sbjct: 301 LGKMVFTVTSLGSGFKGGEVTPLFYIGATLGNALAPLLHLPFPLLAGIGFVAVFAGAANT 360

Query: 361 PIASTIMAIELFGPDIGPFAALACVVAYLFSGHTGIYHAQRVSQTKHVPLPEGIKIAEVN 420
           P+ASTIMAIELFGP IGPFAALACVVAYLFSGHTGIYHAQRV Q K   LP+G++I EV 
Sbjct: 361 PLASTIMAIELFGPQIGPFAALACVVAYLFSGHTGIYHAQRVGQAKLGLLPKGLRIGEVG 420

Query: 421 AYKTQRKRELEQAAAE 436
            Y    +R+L  A AE
Sbjct: 421 GY----RRKLSAAPAE 432


>gi|300311435|ref|YP_003775527.1| chloride channel protein
           [Herbaspirillum seropedicae SmR1]
          Length = 555

 Score = 66.6 bits (161), Expect = 4e-12,   Method: Compositional matrix adjust.
 Identities = 104/410 (25%), Positives = 170/410 (41%), Gaps = 36/410 (8%)

Query: 52  PWILW-LLPLGGFAVGWLYLHTGKPVEAGNN-LLIDEIHDPKKVIPLRMVPLVLFGTVMS 109
           PW    LLP  G  V  L+L   K   AGN    ++ +      IP+R   L    ++ S
Sbjct: 45  PWYTRVLLPTCGGVVAGLFLVWAKRTPAGNGGDYMEAVAIGDGAIPVRNTLLRSISSLAS 104

Query: 110 HLFGASVGREGTAVQMGGALADQLTHVLRLRQEDRRLLLMAGISAGFASVFGTPMAGALF 169
              G S+GREG  VQ+    A  +           RLL+  G +AG  S +  P+AGA F
Sbjct: 105 IASGGSIGREGPMVQLSALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGAFF 164

Query: 170 GLEVLAIGRMRYDAILPCFVSAIVADQVGLAWGVHHTHYAMSGSAPIAWWSVGAVIVAGV 229
             E++ +G +  ++  P  V+++VA+        +   Y M     I  W V   +V GV
Sbjct: 165 ITEIV-LGSLVMESFGPVVVASVVANITMRELPGYRAAYEMPYFPEIGGWEVMLFLVLGV 223

Query: 230 VFGLVGMVFANSTHRLSAFMKRHVSYAPLRPFIGGL------VVALAVWAVGTHRYIGLG 283
           + G++   F            +     P+R  +GGL      V    VW  G        
Sbjct: 224 LAGVLAPQFLRVLELGKHGFGKLRLPLPVRLGVGGLLVGLISVQVPEVWGNGYS------ 277

Query: 284 IPVIVEAFQHPLAPWD-----FLGKMLFTVTSLGTGFKGGEVTPLFYIGATLG------- 331
              +V +  H    W       + K+L T  ++G+G  GG  TP  ++GA +G       
Sbjct: 278 ---LVNSLLHTSWVWQAVLMVLVVKVLATSITVGSGAVGGIFTPTLFVGAAVGYLFGDAA 334

Query: 332 NAMAPL-LHLPFSMLAGIGFVAVFAGAANTPIASTIMAIEL-FGPDIGPFAALACVVAYL 389
            A+ P+ +  PF+  A +G  A  A A+  P+ + +M  E+     +     L+CVVAY+
Sbjct: 335 QALLPVHISQPFAY-AMVGMGAFLAAASYAPLMAILMIFEMTLSYQVVLPLMLSCVVAYV 393

Query: 390 FSGHTGIYHAQRVSQTKHVPLPEGIKIAEVNAYKTQRKREL---EQAAAE 436
            +          ++  +H    E +++   +  +  R  E    E+A+ E
Sbjct: 394 VARSVDGRSMYEITLKRHRDNEERLRLRGTHMSELIRPAETVLHEEASLE 443


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.326    0.141    0.446 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,337,565
Number of extensions: 54030
Number of successful extensions: 268
Number of sequences better than 1.0e-08: 4
Number of HSP's gapped: 262
Number of HSP's successfully gapped: 5
Length of query: 441
Length of database: 1,619,870
Length adjustment: 88
Effective length of query: 353
Effective length of database: 1,203,190
Effective search space: 424726070
Effective search space used: 424726070
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 132 (55.5 bits)