BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2136.p (808 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311789|ref|YP_003775881.1| chemotaxis histidine kinase [He... 1452 0.0 gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [He... 107 5e-24 gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbas... 93 1e-19 gi|300312286|ref|YP_003776378.1| signal-transducing histidine ki... 91 4e-19 gi|300313133|ref|YP_003777225.1| CheA-like histidine kinase [Her... 91 5e-19 >gi|300311789|ref|YP_003775881.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 808 Score = 1452 bits (3760), Expect = 0.0, Method: Compositional matrix adjust. Identities = 744/808 (92%), Positives = 775/808 (95%) Query: 1 MTIRMRITLLVILTFVAIAGIGGYAMYQSRSSAVEVRSVTEGVMPSVLASADLMGQLKDV 60 MTIRMRITLLVILTF+AIAGIGGYAMYQSRSSAVEVRSVTEGVMPSVLASADLMGQLKDV Sbjct: 1 MTIRMRITLLVILTFIAIAGIGGYAMYQSRSSAVEVRSVTEGVMPSVLASADLMGQLKDV 60 Query: 61 QLAAMVMVTESDNQLVAQENEKLADKKAVLEKALAAQAQQAEGVAQRGLIEQAKESLDNY 120 QLAAMVMVTE+DNQLVAQENEKLA+KKAVLEKALAAQAQQAEGVAQRGLIEQAKESLDNY Sbjct: 61 QLAAMVMVTENDNQLVAQENEKLAEKKAVLEKALAAQAQQAEGVAQRGLIEQAKESLDNY 120 Query: 121 FNAIDTTAKFKLAGKNEIAQAQFFGGVMQYQTELEGIVNTLRIEKNRFKDNAIASLNGKL 180 F AIDTTAKFKLAGKNEIAQAQFFGGVMQYQTELEGIVNTLRIEKNRFKDNAIASLNGKL Sbjct: 121 FGAIDTTAKFKLAGKNEIAQAQFFGGVMQYQTELEGIVNTLRIEKNRFKDNAIASLNGKL 180 Query: 181 AQTTTTISIVTVVAVLLLGGIGLLLYRQITGPISNMAAMMGEIATSQDFTRRLPVQRMDE 240 AQTTTTISIVTV+AVLLLGGIG LLYRQITGPIS MAAMMGEIATSQDFTRRLPVQRMDE Sbjct: 181 AQTTTTISIVTVMAVLLLGGIGFLLYRQITGPISRMAAMMGEIATSQDFTRRLPVQRMDE 240 Query: 241 VGHSIVAFNGMVEKIEQASAQVRQKTADMQAMLQNIPQGILTIVEGNKVHPEYSAFLETV 300 VGHSIVAFNGMVEKIE+ASAQVRQKTADMQAMLQNIPQGILTIVEGN++HPEYSAFLETV Sbjct: 241 VGHSIVAFNGMVEKIEEASAQVRQKTADMQAMLQNIPQGILTIVEGNRIHPEYSAFLETV 300 Query: 301 FDTTDIAGRDVMELVFANTNLGADILSQIEAIGGACIGEDVMNFEFNEHLMVGEIEKTMA 360 F+TTDIAGRDVMELVFA+T LGAD LSQIEAIGGACIGEDVMNFEFN HLMVGEIEKTMA Sbjct: 301 FETTDIAGRDVMELVFADTQLGADTLSQIEAIGGACIGEDVMNFEFNAHLMVGEIEKTMA 360 Query: 361 DGSVKILDLSWSPITDEADMVIRLMLCVRDVTELRQLAAEANEQKRELEMIGEILAVAPA 420 DGS KILDLSWSPITDEAD VIRLMLCVRDVTELRQLAAEANEQKRELEMIGEILAV PA Sbjct: 361 DGSKKILDLSWSPITDEADTVIRLMLCVRDVTELRQLAAEANEQKRELEMIGEILAVTPA 420 Query: 421 KFAEFIGSARRFLDENEQIVHQNPQATAFAIAKLFRNMHTIKGNGRTYGLLHLANVVHHT 480 KFAEFI SARRFLDENEQIVHQNP+ATAFAIAKLFRNMHTIKGNGRTYGLLHLANVVHHT Sbjct: 421 KFAEFISSARRFLDENEQIVHQNPEATAFAIAKLFRNMHTIKGNGRTYGLLHLANVVHHT 480 Query: 481 EQHYDDLRHASSGAVWNQEELLLQLAQVREVIDRYARINDVTLGRGNNAGGDQVAAGYER 540 EQHYDDLRH +SGAVW QEELL QLAQVRE+IDRYARINDVTLGRG A G+Q GY R Sbjct: 481 EQHYDDLRHPASGAVWRQEELLRQLAQVREIIDRYARINDVTLGRGVAAAGEQTLPGYAR 540 Query: 541 HVLTIDRKHIQDSLLRLENVNTANLHELVAVRNDVRRTLRLLGTATIEDVLAPVIESLPA 600 HV+TIDRKHI+ SL RLENVNTANLHELVAVRNDVRRTLRLLGT TI++VLAPV +SLPA Sbjct: 541 HVITIDRKHIEQSLERLENVNTANLHELVAVRNDVRRTLRLLGTETIQEVLAPVTDSLPA 600 Query: 601 LAADLGKAAPVVSIDDNGYVVHSFASPMLQNVFMHLIRNAMDHGLESTEERLAKGKPEAG 660 LAADLGKAAPVV I+DNGYVVHSFA+P+LQNVFMHLIRNAMDHGLES+EERLAKGKPEAG Sbjct: 601 LAADLGKAAPVVQIEDNGYVVHSFAAPLLQNVFMHLIRNAMDHGLESSEERLAKGKPEAG 660 Query: 661 TIKLEMGVMDDMFQMALSDDGRGLALHKVRQIGIEKNLISIDQALTDAEIARLILQPGFS 720 IKLEMGVM+DMFQMALSDDGRGLAL +VRQIGIEK+LI++DQAL+DAEIARLILQPGFS Sbjct: 661 VIKLEMGVMEDMFQMALSDDGRGLALERVRQIGIEKHLITVDQALSDAEIARLILQPGFS 720 Query: 721 TRSEVTEVSGRGVGMDAVLDFVTREHGQIEIRFTDTHEGAALRPFQIIVMLPENVAVEID 780 TRSEVTEVSGRGVGMDAVLDFVTREHGQIEIRFTDT EGA R FQIIVMLPENVAVE+D Sbjct: 721 TRSEVTEVSGRGVGMDAVLDFVTREHGQIEIRFTDTEEGAPFRSFQIIVMLPENVAVEVD 780 Query: 781 GIDVPYPVGAQEVLQPVVLPQGGDSQVA 808 GIDVPYP+G QE +QPVVLPQGGDSQVA Sbjct: 781 GIDVPYPMGGQETVQPVVLPQGGDSQVA 808 >gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 725 Score = 107 bits (266), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 72/203 (35%), Positives = 106/203 (52%), Gaps = 24/203 (11%) Query: 601 LAADLGKAAPVVSIDDNGYVVHSFASPMLQNVFMHLIRNAMDHGLESTEERLAKGKPEAG 660 +A DLGK +V + + S L + MH++RNA+DHG+E ERLA GKP Sbjct: 410 IARDLGKDIDLVMTGSDTELDKSMVE-RLADPLMHIVRNAIDHGIEPAAERLAAGKPAKA 468 Query: 661 TIKLEMGVMDDMFQMALSDDGRGLALHKVRQIGIEKNLISIDQALTDAEIARLILQPGFS 720 +++L + + DDGRG+ + + + L S D L+DAEI R + +PGFS Sbjct: 469 SLQLNAIHESGSVVIEVMDDGRGMDREAILAKAVAQGLASPDAELSDAEIYRFVFEPGFS 528 Query: 721 TRSEVTEVSGRGVGMDAVLDFVTREHGQIEIRFTDTHEG-------------AALRPFQI 767 T +VTE+SGRGVGMD V + HG++ I DT G A + FQ+ Sbjct: 529 TARQVTELSGRGVGMDVVRRNIEALHGEVSI---DTGRGRGTVVRLRLPLTLAIISGFQV 585 Query: 768 IV-----MLPENVAVEIDGIDVP 785 +V ++P ++ VE ID+P Sbjct: 586 VVGNAVFVIPLDMVVEC--IDMP 606 >gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbaspirillum seropedicae SmR1] Length = 638 Score = 92.8 bits (229), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 60/158 (37%), Positives = 84/158 (53%), Gaps = 11/158 (6%) Query: 601 LAADLGKAAPVV----SIDDNGYVVHSFASPMLQNVFMHLIRNAMDHGLESTEERLAKGK 656 LA DLGK +V + + VV S P++ H++RN++DHG+E +R+A GK Sbjct: 338 LAKDLGKDVDLVITGAETEVDRVVVDSLYDPLV-----HMLRNSLDHGIEV--DRVAAGK 390 Query: 657 PEAGTIKLEMGVMDDMFQMALSDDGRGLALHKVRQIGIEKNLISIDQALTDAEIARLILQ 716 P TI L + + DDG+G+ +R + K LI+ QALT E +L+ Sbjct: 391 PTKATISLNAWQAGSSVMIEVFDDGKGMDPDMLRSKALAKGLINEHQALTADEALQLVFL 450 Query: 717 PGFSTRSEVTEVSGRGVGMDAVLDFVTREHGQIEIRFT 754 PGFST+ + VSGRGVGMD V V + G I I T Sbjct: 451 PGFSTKEVASSVSGRGVGMDVVKTAVEKHRGTIRIEST 488 >gi|300312286|ref|YP_003776378.1| signal-transducing histidine kinase CheA protein [Herbaspirillum seropedicae SmR1] Length = 697 Score = 90.5 bits (223), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 50/118 (42%), Positives = 72/118 (61%), Gaps = 1/118 (0%) Query: 635 HLIRNAMDHGLESTEERLAKGKPEAGTIKLEMGVMDDMFQMALSDDGRGLALHKVRQIGI 694 HL+RN++DHG+E + R A GK EAGT+ L + ++DDG GL K+ Sbjct: 419 HLVRNSIDHGIELPDARRASGKSEAGTLSLSAEHQGGNIIIEVTDDGGGLNREKILAKAK 478 Query: 695 EKNLISIDQALTDAEIARLILQPGFSTRSEVTEVSGRGVGMDAVLDFVTREHGQIEIR 752 ++ + D L+DAE+ +LI +PGFST +VT+VSGRGVGMD V + G ++IR Sbjct: 479 QQGMPVTDN-LSDAEVWQLIFEPGFSTAEQVTDVSGRGVGMDVVKRNILAMGGAVDIR 535 >gi|300313133|ref|YP_003777225.1| CheA-like histidine kinase [Herbaspirillum seropedicae SmR1] Length = 744 Score = 90.5 bits (223), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 55/150 (36%), Positives = 84/150 (56%), Gaps = 7/150 (4%) Query: 629 LQNVFMHLIRNAMDHGLESTEERLAKGKPEAGTIKLEMGVMDDMFQMALSDDGRGLALHK 688 L HL+RNA HG+ES E+RLA+GKP G + ++ + + +SDDGRGL L Sbjct: 457 LMGPLEHLLRNAAVHGIESAEQRLAEGKPAVGRLTVQATHEGNEAVIRVSDDGRGLDLEA 516 Query: 689 VRQIGIEKNLISIDQALTDAEIARLILQPGFSTRSEVTEVSGRGVGMDAVLDFVTREHGQ 748 +RQ +E + +A + A +A LI QPG ST +V+ ++GRG+GMD V V G Sbjct: 517 IRQKALELGAAGLAEA-SQARLADLIFQPGLSTSPQVSALAGRGIGMDVVRSEVAALGGW 575 Query: 749 IEIRFTDTHEGAALRPFQIIVMLPENVAVE 778 + + + +G Q + LP ++AV+ Sbjct: 576 MRV-HSQPGQG-----VQFTLYLPLSLAVQ 599 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,211,503 Number of extensions: 89777 Number of successful extensions: 402 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 412 Number of HSP's successfully gapped: 11 Length of query: 808 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 715 Effective length of database: 1,179,515 Effective search space: 843353225 Effective search space used: 843353225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 135 (56.6 bits)