BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2125.p (479 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311800|ref|YP_003775892.1| two component heavy metal senso... 805 0.0 gi|300309362|ref|YP_003773454.1| transmembrane sensory transduct... 207 2e-54 gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance ... 184 1e-47 gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine ... 108 1e-24 gi|300313819|ref|YP_003777911.1| two component sensor histidine ... 101 1e-22 >gi|300311800|ref|YP_003775892.1| two component heavy metal sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 467 Score = 805 bits (2079), Expect = 0.0, Method: Compositional matrix adjust. Identities = 398/464 (85%), Positives = 422/464 (90%) Query: 16 RRLPLRQSITARLVLMSAASALLIFAVAGVALYTVLKGELLRHERDGLITTLNDISYQIE 75 R LPLRQSITARLV MSAASALLIFA+ G+ LYTVLKGELLRHERDGL+TTLNDISYQI Sbjct: 4 RWLPLRQSITARLVWMSAASALLIFALTGMVLYTVLKGELLRHERDGLVTTLNDISYQIG 63 Query: 76 RAGNTERWSRVQMKLAALSQGDQHVFFWIASDNAQFTFGDLTRLNPAAISHDDGKSVGRL 135 RAGNTERW RVQ KLA LSQ DQHV FWI+S +A+FT+GDL+RL P AI D+G SVGRL Sbjct: 64 RAGNTERWGRVQAKLATLSQADQHVSFWISSSDARFTYGDLSRLAPQAIRADEGNSVGRL 123 Query: 136 HLPGRRYPLHIMTRTLPALDERPEVRVTVGVDGAPYFEMRRTFLLATTISSLLGIVLAVI 195 HLPGRRYPLHIMTRTLPA +ERP V VTVGVDG PYFEMRRTFLLAT++ SLLGIVLAVI Sbjct: 124 HLPGRRYPLHIMTRTLPAFEERPAVDVTVGVDGEPYFEMRRTFLLATSVFSLLGIVLAVI 183 Query: 196 SSYWIARAGLAPLRRMSARAQSFSPRKLSARMPVEALPEELSVLADAFNGALERIEQAYT 255 SSYWIARAGLAPLR+MS RAQSFSPRKLSARMPVEA+P+ELSVLA AFNGAL+RIE AYT Sbjct: 184 SSYWIARAGLAPLRQMSDRAQSFSPRKLSARMPVEAMPQELSVLALAFNGALDRIEDAYT 243 Query: 256 QLEAFNADVAHELRTPLANLIGQTQVALARERSAGEFKDVLQSNLEELDRIRSIVNDMLF 315 QLEAFNADVAHELRTPLANLIG+TQVALARERSAGEFKDVLQSNLEELDRIRSIVNDMLF Sbjct: 244 QLEAFNADVAHELRTPLANLIGETQVALARERSAGEFKDVLQSNLEELDRIRSIVNDMLF 303 Query: 316 LARSDQGEAATARVPARVADEVHKTADFFEFVLDDAGMGITVTGDTTTTATMETALFKRA 375 LARSDQGEAATARVPARVADEV KT DFFEFVLDD+GM I V GDT TA METALFKRA Sbjct: 304 LARSDQGEAATARVPARVADEVQKTVDFFEFVLDDSGMRIEVAGDTEATAMMETALFKRA 363 Query: 376 LTNLIQNAIQHSPQGAQLRIDIASGQDMVSVAVTNPGPAIDEQHLPRLFDRFYRVDSARQ 435 LTNLIQNAIQHSP+GA LRIDIA ++ V VAV+NPG IDE HLPRLFDRFYRVDSARQ Sbjct: 364 LTNLIQNAIQHSPEGALLRIDIACQEETVRVAVSNPGKPIDEHHLPRLFDRFYRVDSARQ 423 Query: 436 DINGSHGHGLGLAIVKAVARMHGGRAFASSSDGHNTIGFTIARQ 479 DINGSHGHGLGLAIVKAVARMHGGRAFASS +G N+IGFTIARQ Sbjct: 424 DINGSHGHGLGLAIVKAVARMHGGRAFASSKEGVNSIGFTIARQ 467 >gi|300309362|ref|YP_003773454.1| transmembrane sensory transduction histidine kinase for cobalt/zinc/ cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 467 Score = 207 bits (527), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 150/483 (31%), Positives = 248/483 (51%), Gaps = 67/483 (13%) Query: 23 SITARLVLMSAASALLIFAVAGVALYTVLKGELLRHERDGLITTLNDISYQIERAGNTER 82 S++ RLV + A++I A G L L +L R + + L I++ +E A + E Sbjct: 8 SLSLRLVALFGLLAMVILAALGTYLSHSLHVQLERRDDEELAGKCRSINHLVEHAASLED 67 Query: 83 -WSRVQMKLAALSQGDQHVFFWIASDNAQFTFGDLTRLNPAAISHDDGKSVGRLHLPGRR 141 +R + L L+ DQ + I+ DG ++ H P Sbjct: 68 ILARRHLVLDTLTGHDQLL---------------------VRIARADGSTLLESHDP--- 103 Query: 142 YPLHIMTRTLPALD-ERPEVRVTVGVDGAPYFEMR------------------------- 175 TRT P +D + ++R + ++R Sbjct: 104 ------TRTPPGIDLSQVDIRAWDSIQSWKLGDLRARYVASKPVTRSGEQAVVLVVRTAS 157 Query: 176 -RTFLLATTISSL-----LGIVLAVISSYWIARAGLAPLRRMSARAQSFSPRKLSARMPV 229 R LLA S + +G +LA++ SY++ R+GL PLR M+A+ ++ S +L R+ + Sbjct: 158 DRMLLLAEYRSDVWWAAGVGALLAILMSYFLVRSGLRPLRTMAAQTRAVSANRLDTRLDL 217 Query: 230 EALPEELSVLADAFNGALERIEQAYTQLEAFNADVAHELRTPLANLIGQTQVALARERSA 289 +A P EL + ++FN L+R+ +++ QL F+AD+AH++RTPL NL+ QTQVAL + R Sbjct: 218 QAAPRELHEIVESFNEMLDRLHRSFQQLSQFSADLAHDVRTPLNNLMVQTQVALGKPRGI 277 Query: 290 GEFKDVLQSNLEELDRIRSIVNDMLFLARSDQGEAATARVPARVADEVHKTADFFEFVLD 349 E++ +L SN+EE +R+ ++ MLFLAR++ VA E+ + AD+FE + D Sbjct: 278 DEYQALLSSNIEEYERLSRMMESMLFLARAENAHVVLQWQELDVAQELTRIADYFEGIAD 337 Query: 350 DAGMGITVTGDTTTTATMETALFKRALTNLIQNAIQHSPQGAQLRIDIASGQDMVSVAVT 409 +A + + V G A + L +RA+ NL+ NAI+++P +R+ + + D + ++V+ Sbjct: 338 EAQVRLVVQGAGRLQA--DATLLRRAVGNLVANAIRYTPADGVIRLQVDAAGDGMEISVS 395 Query: 410 NPGPAIDEQHLPRLFDRFYRVDSARQDINGSHGHGLGLAIVKAVARMHGGRAFASSSDGH 469 NPG I + L RLFDRFYR D +R + GLGLAIV+++ +HGG+A +SS G Sbjct: 396 NPGAGIPAEQLERLFDRFYRADPSRS--SAGGSSGLGLAIVRSIMMLHGGQASVTSSPGQ 453 Query: 470 NTI 472 T+ Sbjct: 454 LTV 456 >gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 184 bits (467), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 102/255 (40%), Positives = 153/255 (60%), Gaps = 4/255 (1%) Query: 207 PLRRMSARAQSFSPRKLSARMPVEALPEELSVLADAFNGALERIEQAYTQLEAFNADVAH 266 PL MS RA +P LSAR+ E +P EL +A +FN L+R+E Y L F+ D+AH Sbjct: 201 PLEAMSQRAAQITPDHLSARLDQEGMPAELRRVALSFNAMLDRLEAGYAHLAQFSGDLAH 260 Query: 267 ELRTPLANLIGQTQVALARERSAGEFKDVLQSNLEELDRIRSIVNDMLFLARSDQGEAAT 326 E+RTP+ L+GQ+QVAL + RS E++ VL+SN+EEL R+ IV ++LFLA++D A Sbjct: 261 EIRTPINVLMGQSQVALGQRRSPEEYEQVLESNVEELQRLSRIVENILFLAQADHAALAV 320 Query: 327 ARVPARVADEVHKTADFFEFVLDDAGMGITVTGDTTTTATMETALFKRALTNLIQNAIQH 386 R +A+E+ + D+FE + D+ GM V A + +++R ++NL+ NA+++ Sbjct: 321 ERNALDLAEELGRITDYFEGIADERGMRFDVQAAGQCQANL--IMWRRVVSNLVINAVRY 378 Query: 387 SPQGAQLRIDIASGQDMVSVAVTNPGPAIDEQHLPRLFDRFYRVDSARQDINGSHGHGLG 446 + +RI S + + V N + Q + R+FDRFYR D +R S +GLG Sbjct: 379 GRAESTIRILAESDGEGSRIMVENRSEDVTAQAMARMFDRFYRGDRSRSAFTES--NGLG 436 Query: 447 LAIVKAVARMHGGRA 461 LAIVKA+ +HGG A Sbjct: 437 LAIVKAIMALHGGTA 451 >gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 444 Score = 108 bits (269), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 92/279 (32%), Positives = 140/279 (50%), Gaps = 36/279 (12%) Query: 199 WIARA-GLAPLRRMSARAQSFSPRKLSARMPVE--ALPEELSVLADAFNGALERIEQAYT 255 W+A + GLAP+R+ +A + R +++ P+ +P+E+ L A N L R++ A Sbjct: 158 WVAVSRGLAPVRKAAA---EVAARDMNSLQPLADVGMPQEIQPLTHALNDLLARLKHATE 214 Query: 256 QLEAFNADVAHELRTPLANLIGQTQV---ALARERSAGEFKDVLQSNLEELDRIRSIVND 312 AF AD AHELRTPL L QTQ+ A+ E F D L++ LE R +V+ Sbjct: 215 TQRAFVADAAHELRTPLTALRLQTQLAERAVDEEERRAAFAD-LKNGLE---RTTHLVHQ 270 Query: 313 MLFLARSDQGEAATARVPAR-----VADEVHKTADFFEFVL-----DDAGMGITVTGDTT 362 +L LAR + + +V R V ++ A+ L D G + VTG+ Sbjct: 271 LLTLARQEPDAMSFTQVDLRALLGSVVGDLSSVAEHGGIDLGLSDQGDDGRPLRVTGNAD 330 Query: 363 TTATMETALFKRALTNLIQNAIQHSPQGAQLRIDI-ASGQDMVSVAVTNPGPAIDEQHLP 421 + NL+ NA++++P G + + + S +V V + GP I E LP Sbjct: 331 ALRIL--------FNNLVDNALRYTPAGGVVDVGVDLSTAGYCTVVVQDSGPGIAESELP 382 Query: 422 RLFDRFYRVDSARQDINGSH---GHGLGLAIVKAVARMH 457 R+FDRFYR + Q+ +GS G GLGLAIV+ +A +H Sbjct: 383 RVFDRFYRAQHS-QEGSGSRAAFGSGLGLAIVRQIADLH 420 >gi|300313819|ref|YP_003777911.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 485 Score = 101 bits (251), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 83/295 (28%), Positives = 139/295 (47%), Gaps = 16/295 (5%) Query: 174 MRRTFLLATTISSLLGIVLAVISSYWIARAGLAPLRRMSARAQSFSPRKLSARMPVEALP 233 M R L T + +A + ++ R L PL ++ +S +P LS + Sbjct: 167 MTRKILFDTLWRQGTLVTVAALLVWFAVRFVLRPLMQLKGEVESRAPTDLSG-FDTALVH 225 Query: 234 EELSVLADAFNGALERIEQAYTQLEAFNADVAHELRTPLANLIGQTQVALARER----SA 289 +E+ L A NG + R++ + F AD +H+LRTPL L Q+++ L R A Sbjct: 226 KEMRPLVQAMNGYMGRLQALISAQRRFIADASHQLRTPLTVLKTQSELTLRELRREQVDA 285 Query: 290 GEFKDVLQSNLEELDRIRSIVNDMLFLARSDQGEAATARVPARVADEVHKTADFF--EFV 347 E +D+++ D + N +L LAR++ G A +P ++D + A + V Sbjct: 286 AELRDLVEGMARTTDAAVHLANRLLTLARAEHGAAEGGTMPLSLSDAARQVALELSQQAV 345 Query: 348 LDDAGMGITVTGDTTTTATMETALFKRALTNLIQNAIQHSPQGAQLRIDI----ASGQDM 403 + G+ GD + A L + NL+ NAI+++P G + + + + GQ Sbjct: 346 SREIGLAFEEEGDVSIEAN--ALLLHELMVNLLDNAIRYTPAGGHVTLRVRAQSSPGQPT 403 Query: 404 VS-VAVTNPGPAIDEQHLPRLFDRFYRVDSARQDINGSHGHGLGLAIVKAVARMH 457 V+ + V + GP I ++F+ FYR A Q +N G GLGL+IV+ +A MH Sbjct: 404 VALLEVEDSGPGIPAAEREKVFEPFYRA-GATQHVNPG-GTGLGLSIVREIAAMH 456 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.133 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,440,267 Number of extensions: 60514 Number of successful extensions: 391 Number of sequences better than 1.0e-08: 34 Number of HSP's gapped: 339 Number of HSP's successfully gapped: 34 Length of query: 479 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 390 Effective length of database: 1,198,455 Effective search space: 467397450 Effective search space used: 467397450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)