BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2112.p (565 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311812|ref|YP_003775904.1| glucose-6-phosphate isomerase [... 961 0.0 gi|300310432|ref|YP_003774524.1| glucose-6-phosphate isomerase [... 552 e-158 >gi|300311812|ref|YP_003775904.1| glucose-6-phosphate isomerase [Herbaspirillum seropedicae SmR1] Length = 570 Score = 961 bits (2485), Expect = 0.0, Method: Compositional matrix adjust. Identities = 457/555 (82%), Positives = 496/555 (89%) Query: 1 MTKLPTADLQLPLFVSSQRESLTDKSAWIALQEHYQDSARHFQLERLFAADLQRGQSLVA 60 MT LP ADLQLPLFV +QRESLT KSAW +LQEHY+D ARHFQL RLFAAD +RG+SL A Sbjct: 1 MTTLPAADLQLPLFVYAQRESLTGKSAWRSLQEHYRDRARHFQLARLFAADDKRGESLTA 60 Query: 61 EGAGIYLDYSKNLVSGQTMSLLIQLARQCGLQDRIEAMFRGDRINVTEQRAVLHTALRKP 120 E GIYLDYSKNLV+ +TM+LL+QLARQCGL DRIEAMFRGDRIN+TE R+VLHTALRKP Sbjct: 61 EAVGIYLDYSKNLVTSETMALLMQLARQCGLPDRIEAMFRGDRINLTENRSVLHTALRKP 120 Query: 121 AGEQLVVEGEEVVAQVQQVLTRMEAFAEQVRSGQWTGHTGGRIRHVVNIGIGGSDLGPSM 180 A E+LVV+G++VVA+VQQVL RME FA QVRSGQW GHTG IRHVVNIGIGGSDLGP M Sbjct: 121 ASERLVVDGQDVVAEVQQVLRRMEGFARQVRSGQWLGHTGLPIRHVVNIGIGGSDLGPMM 180 Query: 181 AQEALRAYSQHDLNLHFLSDVDGTALLETVRTLPAQETLFIVCSKTFTTLETLTNAQAAR 240 A EALR YS DL LHF+SDVDG A+L+TVR LPA++TLFIVCSKTFTTLET+TNA AAR Sbjct: 181 AYEALRPYSHDDLTLHFVSDVDGAAMLDTVRGLPAEQTLFIVCSKTFTTLETITNATAAR 240 Query: 241 QWLVQQLGDEAAVARHFVGVTSNIEEASRFGIDPTNMFGFWDWVGGRYSMTSAIGLSTMI 300 WLVQQLG E AVARHFVGVT+NIEEA RFGID NMFGFWDWVGGRYSMTSAIGLSTM+ Sbjct: 241 AWLVQQLGAEEAVARHFVGVTTNIEEAQRFGIDADNMFGFWDWVGGRYSMTSAIGLSTMV 300 Query: 301 AIGAQHFRQLLAGFHAMDEHFRTTPLEANLPVILGMLTVWYTNFFGARSQAVLPYSQSLK 360 AIG +HFR +L GFHAMDEHFR+ PLE+NLPVILGMLTVWYTNFFGA SQAVLPYSQ LK Sbjct: 301 AIGPEHFRAMLEGFHAMDEHFRSAPLESNLPVILGMLTVWYTNFFGAASQAVLPYSQHLK 360 Query: 361 RLPAYLQQLTMESNGKQVTRQGQSVHYQTSPVYWGDNGINGQHSFYQLLHQGTQMVPCDF 420 RLPAYLQQLTMESNGK +T G+ V YQTSPVYWGDNGINGQHSFYQ+LHQGTQ+VPCDF Sbjct: 361 RLPAYLQQLTMESNGKHITVNGEPVDYQTSPVYWGDNGINGQHSFYQMLHQGTQLVPCDF 420 Query: 421 IGFCRPVQALGQQHALLMANMFAQSEALAFGKTAQQVRQEGSSEELTPHRVFPGNRPSNS 480 IG C+P QALGQQH LLMANMFAQSEALAFGK A QVRQEG+ E L PHRVF GNRPSNS Sbjct: 421 IGLCQPQQALGQQHELLMANMFAQSEALAFGKDADQVRQEGTPEALVPHRVFEGNRPSNS 480 Query: 481 LLIDQLTPYSLGTLVALYEHSVFTQGVIWDIDSFDQWGVELGKQLARRTARELIDHAQPL 540 +L+ QLTPYSLGTLVALYEHSVFTQGV+W+IDSFDQWGVELGKQLA+RTARELIDH QPL Sbjct: 481 MLLQQLTPYSLGTLVALYEHSVFTQGVVWNIDSFDQWGVELGKQLAKRTARELIDHQQPL 540 Query: 541 HHDSSTNALIHRYRA 555 HHDSSTNALI RYRA Sbjct: 541 HHDSSTNALIRRYRA 555 >gi|300310432|ref|YP_003774524.1| glucose-6-phosphate isomerase [Herbaspirillum seropedicae SmR1] Length = 554 Score = 552 bits (1422), Expect = e-158, Method: Compositional matrix adjust. Identities = 282/547 (51%), Positives = 359/547 (65%), Gaps = 12/547 (2%) Query: 21 SLTDKSAWIALQEHYQDSARHFQLERLFAADLQRGQSLVAEGAGIYLDYSKNLVSGQTMS 80 +LTD +A+ ALQ H+ + + + LFA R + + AG+ LDYSKN + +T++ Sbjct: 5 ALTDTAAFKALQHHHAKAVT-WHMRELFAQQPDRFERMHVRAAGLLLDYSKNRIESETLA 63 Query: 81 LLIQLARQCGLQDRIEAMFRGDRINVTEQRAVLHTALRKPA-GEQLVVEGEEVVAQVQQV 139 LL+ LAR+ G++ EAMFRG++IN TE RAVLHTALR PA V+G+ V +V V Sbjct: 64 LLMSLARERGVEALREAMFRGEKINFTEHRAVLHTALRAPAQACDAQVDGQPVSQEVHAV 123 Query: 140 LTRMEAFAEQVRSGQWTGHTGGRIRHVVNIGIGGSDLGPSMAQEALRAYSQHDLNLHFLS 199 L + F+E+VRSG W G+TG I +VNIGIGGS LGP M ALR Y L HF+S Sbjct: 124 LDHIRDFSERVRSGAWRGYTGREITDIVNIGIGGSFLGPQMVCTALRNYRHARLTTHFVS 183 Query: 200 DVDGTALLETVRTLPAQETLFIVCSKTFTTLETLTNAQAARQWLVQQLGDEAAVARHFVG 259 +VDG L E + + + TLFIV SKTFTT ET+ NA++AR W ++ G +A +ARHFV Sbjct: 184 NVDGHDLDEVLSRVDPETTLFIVASKTFTTQETMLNAKSARAWFLRH-GQQADLARHFVA 242 Query: 260 VTSNIEEASRFGIDPTNMFGFWDWVGGRYSMTSAIGLSTMIAIGAQHFRQLLAGFHAMDE 319 V++N E FGIDP NMF FWDWVGGRYS+ SAIGL + IG +HF LAG HAMD Sbjct: 243 VSTNAAEVESFGIDPDNMFPFWDWVGGRYSVWSAIGLPIALLIGFEHFSAFLAGAHAMDR 302 Query: 320 HFRTTPLEANLPVILGMLTVWYTNFFGARSQAVLPYSQSLKRLPAYLQQLTMESNGKQVT 379 HFR PLEAN+PVI ++ +W NFF + S +V PY Q L+ PAYLQQL MESNGK++T Sbjct: 303 HFRDAPLEANMPVIQALIGIWNRNFFNSESLSVAPYHQWLRYFPAYLQQLDMESNGKRIT 362 Query: 380 RQGQSVHYQTSPVYWGDNGINGQHSFYQLLHQGTQMVPCDFIGFCRPVQALGQQHALLMA 439 R G V T P WGD G NGQH+F+QLLHQGT + P DFI +P + + +L+A Sbjct: 363 RDGTPVGVATCPFIWGDVGTNGQHAFFQLLHQGTNVTPIDFIAVLKPSHGIDEHQDVLLA 422 Query: 440 NMFAQSEALAFGKTAQQVRQEGSSE--------ELTPHRVFPGNRPSNSLLIDQLTPYSL 491 N FAQSEA GK A +VR + + L PH+ F GNRPSN+LL+ +L+P++L Sbjct: 423 NCFAQSEAFMRGKQADEVRADLERQGVDAAELAALLPHKTFDGNRPSNTLLLPELSPHAL 482 Query: 492 GTLVALYEHSVFTQGVIWDIDSFDQWGVELGKQLARRTARELIDHAQPLHHDSSTNALIH 551 G ++ALYEH F QG IW I+SFDQWGVELGK LA+ EL P HD+STNALI Sbjct: 483 GAMIALYEHKTFVQGAIWGINSFDQWGVELGKVLAKTIHGELRSEPNPALHDASTNALIA 542 Query: 552 RYR-ARQ 557 R ARQ Sbjct: 543 LARQARQ 549 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,689,157 Number of extensions: 67930 Number of successful extensions: 206 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 202 Number of HSP's successfully gapped: 2 Length of query: 565 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 475 Effective length of database: 1,193,720 Effective search space: 567017000 Effective search space used: 567017000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)