BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2106.p (477 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 922 0.0 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 306 2e-84 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 280 2e-76 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 276 3e-75 gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrog... 276 3e-75 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 922 bits (2383), Expect = 0.0, Method: Compositional matrix adjust. Identities = 448/477 (93%), Positives = 464/477 (97%) Query: 1 MKQHFIDNRWVAPASGQSIPVIDPSDGQVFAAIARGNADDVERAVQAARLAYEGAWGKLS 60 MKQHFI NRWVAP SG+SIPV+DPSDGQ F AIARGNA D+++AV+AARLAYEGAWG+LS Sbjct: 1 MKQHFIANRWVAPTSGESIPVLDPSDGQPFDAIARGNATDIDQAVRAARLAYEGAWGRLS 60 Query: 61 AAERSRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQARADAAAIVRYFEFYAGAADKLHG 120 AAERSRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQARADAAAIVRYFEFYAGAADKLHG Sbjct: 61 AAERSRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQARADAAAIVRYFEFYAGAADKLHG 120 Query: 121 HTIPYQAGYTVLTLREPHGVTAHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSI 180 TIPYQAGYTVLTLREPHGVT HIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSI Sbjct: 121 DTIPYQAGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSI 180 Query: 181 LRVAELAAEVGFPAGALNIVTGYGHEAGDLLVKHPDVDHVSFTGSPNVGKLVVRGAAEHH 240 LRVAELAAEVGFP GALNIVTGYGHEAGDLLVKHPDV+H+SFTGSPNVGKLVVR AAEHH Sbjct: 181 LRVAELAAEVGFPEGALNIVTGYGHEAGDLLVKHPDVNHISFTGSPNVGKLVVRAAAEHH 240 Query: 241 TPVTLELGGKSPQVVFEDADMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQVLERL 300 TPVTLELGGKSPQ+VFEDADMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQVLERL Sbjct: 241 TPVTLELGGKSPQIVFEDADMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQVLERL 300 Query: 301 AGLFSQLKVGSSQMDLDCGPLIRKTQQERVAGFLAEARRDGIPLVAQGEIAAGVPANGFY 360 +GLFSQL+VGSSQMDLDCGPLIRKTQQERVAGFL++A+RDGI L AQG+IAAGVP GFY Sbjct: 301 SGLFSQLQVGSSQMDLDCGPLIRKTQQERVAGFLSDAQRDGIALAAQGKIAAGVPTGGFY 360 Query: 361 QAPALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAIALANGTDYGLVASVWTRDGGRQM 420 QAPALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAIALANGTDYGLVASVWTRDGGRQM Sbjct: 361 QAPALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAIALANGTDYGLVASVWTRDGGRQM 420 Query: 421 RLARAIRSGQVFINNYGAGGGVELPFGGVKASGHGREKGFEALYGFTVLKTIAIRHG 477 RLARAIRSGQVFINNYGAGGGVELPFGGVKASGHGREKGFEALYGFTVLKTIAIRHG Sbjct: 421 RLARAIRSGQVFINNYGAGGGVELPFGGVKASGHGREKGFEALYGFTVLKTIAIRHG 477 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 306 bits (785), Expect = 2e-84, Method: Compositional matrix adjust. Identities = 195/480 (40%), Positives = 274/480 (57%), Gaps = 13/480 (2%) Query: 3 QHFIDNRWVAPASGQSIPVIDPSDGQVFAAIARGNADDVERAVQAARLAYEG-AWGKLSA 61 QHFI + GQ + +++P + +++A+ A+G A DV RAV AA+ EG AW KLS Sbjct: 6 QHFIAGEATEASDGQRMNLVNPINEEIYASAAQGTAADVARAVAAAKAQLEGGAWSKLSG 65 Query: 62 AERSRLLMKLAYKLQDHQEELAQLEGRDCGK-PMKQARADAAAIVRYFEFYAGAADKLHG 120 +R +LL KLA ++ + LA ++ R G+ PM+ D + + AG A+++ G Sbjct: 66 LQRGQLLHKLAALVERDTDLLADMDARAIGRSPMEPRMMDVPNAISHLRAAAGWANQMEG 125 Query: 121 HTIPYQAGYT-----VLTLREPHGVTAHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAED 175 TIP AGY T+REP GV I+PWN P+ I V LAAG T V+KPAE+ Sbjct: 126 RTIP-TAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIKPAEE 184 Query: 176 ACLSILRVAELAAEVGFPAGALNIVTGYGHEAGDLLVKHPDVDHVSFTGSPNVGKLVVRG 235 S L +A LA E GFP G +N+VTGYG+ G L +HPDV +SFTGSP G+ + R Sbjct: 185 TPQSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRAIQRI 244 Query: 236 AAEHHTPVTLELGGKSPQVVFEDADMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQ 295 A E VTLELGGKSPQ+VF+DA E L + N GQ C+AGSR+LVQR ++ Sbjct: 245 AGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRSLAQR 304 Query: 296 VLERLAGLFSQLKVGS-SQMDLDCGPLIRKTQQERVAGFLAEARRDGIPLVAQGEIAAGV 354 + LA + + VG SQ + GP+ +K Q ERV ++ + +G L+A G Sbjct: 305 FAKALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGG---VSR 361 Query: 355 PANGFYQAPALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAIALANGTDYGLVASVWTR 414 P G++ P + D +A+EE+FGPV + F+ E AIALAN + YGL A+VWT Sbjct: 362 PDKGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWTT 421 Query: 415 DGGRQMRLARAIRSGQVFINNYGAGGGVELPFGGVKASGHGREKGFEALYGFTVLKTIAI 474 + R R+A ++ G V +N + + LP+GG+K SG GRE G +T KT+ + Sbjct: 422 NLARAHRVAAGVKVGAVGVNCW-SPLDANLPWGGIKDSGMGREGGLAGALAYTEEKTVTV 480 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 280 bits (715), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 191/473 (40%), Positives = 253/473 (53%), Gaps = 7/473 (1%) Query: 1 MKQHFIDNRWVAPASGQSIPVIDPSDGQVFAAIARGNADDVERAVQAARLAYEGAWGKLS 60 + +ID +W P + V+ P+ + IA GNA DV++AVQAAR A AW S Sbjct: 15 LDHFYIDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAARRAIP-AWAATS 73 Query: 61 AAERSRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQARADAAAIVRYFEFYAGAADKLHG 120 ER+ LL ++ L + E AQ + G P+ AR A + E A D L Sbjct: 74 PLERAALLGRVHALLLERSELFAQALTLEMGAPISYART--AHVPLAAEHLRVARDNLAD 131 Query: 121 HTIPYQAGYTVLTLREPHGVTAHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSI 180 + G T + +REP GV A I PWN+P+ VG ALAAG T V+KP+E + LS Sbjct: 132 YPFIRPRGTTAI-VREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLSA 190 Query: 181 LRVAELAAEVGFPAGALNIVTGYGHEAGDLLVKHPDVDHVSFTGSPNVGKLVVRGAAEHH 240 L AE+ E G PAG N+V G G E G L HP VD +S TGS G LV + AA Sbjct: 191 LLFAEIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAAVTV 250 Query: 241 TPVTLELGGKSPQVVFEDADMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQVLERL 300 V ELGGKSP +V DAD+E +P V A ++N GQ+CSA +RL+V R A +V E Sbjct: 251 KRVAQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALARVHELA 310 Query: 301 AGLFSQLKVGS-SQMDLDCGPLIRKTQQERVAGFLAEARRDGIPLVAQGEIAAGVPANGF 359 +Q+KVG + GPL + Q RV + +DG L+A G A G+ Sbjct: 311 LATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRPDGLAQGY 370 Query: 360 YQAPALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAIALANGTDYGLVASVWTRDGGRQ 419 Y P + +V +AQEE+FGPVLA +P++ AIA+AN T YGL A V RD + Sbjct: 371 YARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGRDKDQV 430 Query: 420 MRLARAIRSGQVFINNYGAGGGVELPFGGVKASGHGREKGFEALYGFTVLKTI 472 +A I+SGQV + NY A + PFGG K SG+GRE G E + + +K I Sbjct: 431 RAVAARIQSGQVHL-NYPAWDP-QAPFGGYKQSGNGREYGVEGMEEYMEVKAI 481 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 276 bits (706), Expect = 3e-75, Method: Compositional matrix adjust. Identities = 170/469 (36%), Positives = 261/469 (55%), Gaps = 23/469 (4%) Query: 4 HFIDNRWVAPASGQSIPVIDPSDGQVFAAIARGNADDVERAVQAARLAYEGAWGKLSAAE 63 +FI ++V P G+ I P G+ F +AR +A+DVE A+ AA A + +WGK S E Sbjct: 21 NFIGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVELALDAAHAA-KKSWGKTSPTE 79 Query: 64 RSRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQARA-DAAAIVRYFEFYAGAADKLHGHT 122 R+ +L+K+A +++ + E LA E D GKP+++ A D + +F ++A A G Sbjct: 80 RANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQEGSI 139 Query: 123 IPYQAGYTVLTLREPHGVTAHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSILR 182 P EP GV I+PWN+P+ + + ALAAGN V+KPAE SI+ Sbjct: 140 CPIDNDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPASIMV 199 Query: 183 VAELAAEVGFPAGALNIVTGYGHEAGDLLVKHPDVDHVSFTGSPNVGKLVVRGAAEHHTP 242 + EL A++ P G +NIV G+G EAG L + + ++FTG G+L+++ A+++ P Sbjct: 200 LIELIADL-IPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYASQNLIP 258 Query: 243 VTLELGGKSPQVVFEDA-------DMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQ 295 VTLELGGKSP + F D +A+ + A+ N G+ C+ SR+LVQ YE+ Sbjct: 259 VTLELGGKSPNIFFADVLDKDDDFFDKALEGFAMFAL--NQGEVCTCPSRVLVQESIYER 316 Query: 296 VLERLAGLFSQLKVGSSQMDLDCGPLI----RKTQQERVAGFLAEARRDGIPLVAQG--E 349 +ER + +K G+ LD +I + Q E++ ++ +++G ++A G E Sbjct: 317 FIERALKRVAAIKQGNP---LDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGGGRE 373 Query: 350 IAAGVPANGFYQAPALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAIALANGTDYGLVA 409 G A+G+Y P + + + R+ QEE+FGPV++ F+DE A+A+AN T YGL A Sbjct: 374 ELGGDLASGYYVKPTVFQG-NNKMRIFQEEIFGPVVSVTTFKDEEEALAIANDTLYGLGA 432 Query: 410 SVWTRDGGRQMRLARAIRSGQVFINNYGAGGGVELPFGGVKASGHGREK 458 +WTRDG R R+ R I++G+V+ N Y FGG K SG GRE Sbjct: 433 GLWTRDGTRAFRMGREIQAGRVWTNCYHLYPA-HAAFGGYKQSGIGREN 480 >gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 504 Score = 276 bits (705), Expect = 3e-75, Method: Compositional matrix adjust. Identities = 161/420 (38%), Positives = 228/420 (54%), Gaps = 5/420 (1%) Query: 56 WGKLSAAERSRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQARADAAAIVRYFEFYAGAA 115 W LS A R R L + ++ E LA+LE GKP++ RA+ + FE+YAG A Sbjct: 75 WWALSHAARGRALYAVGAVIRAEAEPLARLEAISSGKPIRDCRAEMQKVAEMFEYYAGWA 134 Query: 116 DKLHGHTIPYQAGYTVLTLREPHGVTAHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAED 175 DK +G IP + + T REP+G + PWN P G +G ALA GN ++KP+E Sbjct: 135 DKFYGEVIPVPSSHLNYTRREPYGTVLQMTPWNAPAFTCGWQLGPALATGNAVLLKPSEL 194 Query: 176 ACLSILRVAELAAEVGFPAGALNIVTGYGHEAGDLLVKHPDVDHVSFTGSPNVGKLVVRG 235 S L +A L + G PAG +N++ G G + V V F GSP G L+ + Sbjct: 195 TPFSSLAIARLGEQAGLPAGLVNVLAGLGQTMVPQAMATWTVKKVIFVGSPATGALIAKA 254 Query: 236 AAEHHTPVTLELGGKSPQVVFEDADMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQ 295 AA P LELGGKS ++FEDAD+ AI AGQ+C AGSRLLVQR Y++ Sbjct: 255 AAARVMPCVLELGGKSANIIFEDADLRLAAFGAQAAIFSGAGQSCVAGSRLLVQRKVYDR 314 Query: 296 VLERLAGLFSQLKVGSSQMD-LDCGPLIRKTQQERVAGFLAEARRDGIPLVAQGEIAAGV 354 +E +A ++++G+ D + GP+ + Q E + +A G L A G G Sbjct: 315 FVETVAAGAEKIRLGAPLDDSTEVGPINNRKQYEHIQRMVARGLEAGATLAA-GHTRYG- 372 Query: 355 PANGFYQAPALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAIALANGTDYGLVASVWTR 414 G++ P LL +A+ E+FGPV A+PFEDE AIA+AN +++GL +VWTR Sbjct: 373 -EEGYFVRPTLLAHASNAMEVARSEIFGPVAVAIPFEDEEEAIAIANDSEFGLAGAVWTR 431 Query: 415 DGGRQMRLARAIRSGQVFINNYGAGGGVELPFGGVKASGHGREKGFEALYGFTVLKTIAI 474 D R R+A ++ +G ++N+Y V PFGG SG+GR G EALY +T K++ + Sbjct: 432 DVARAHRVAASVNAGTFWVNSYKT-INVASPFGGFNRSGYGRSSGMEALYDYTQTKSVWV 490 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,695,829 Number of extensions: 79490 Number of successful extensions: 335 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 254 Number of HSP's successfully gapped: 21 Length of query: 477 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 388 Effective length of database: 1,198,455 Effective search space: 465000540 Effective search space used: 465000540 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)