BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2105.p (252 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 476 e-136 gi|300313109|ref|YP_003777201.1| short-chain alcohol dehydrogena... 144 5e-36 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 142 2e-35 gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogena... 139 2e-34 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 139 2e-34 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 476 bits (1225), Expect = e-136, Method: Compositional matrix adjust. Identities = 240/252 (95%), Positives = 249/252 (98%) Query: 1 MRLQDKVAIVTGGGSGFGEGIAKAYAREGAAVVVADIGQTGGLRVVEEIQAGGGKAIFAK 60 MRLQDKVAIVTGGGSGFGEGIAKAYAREGAA+VVADIG+ GGLRVVEEI+A GG+A+FAK Sbjct: 1 MRLQDKVAIVTGGGSGFGEGIAKAYAREGAAIVVADIGEAGGLRVVEEIKAAGGRAVFAK 60 Query: 61 ADVSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRPMLEVEEEEFDRVYAVNVKSIFL 120 ADVSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRPMLEVEE+EFDRVYAVNVKSIFL Sbjct: 61 ADVSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRPMLEVEEDEFDRVYAVNVKSIFL 120 Query: 121 SAKTFVPYFRQVGGGTFVNIASTAGIRPRPGLTWYNGSKGAVITTSKSMAAELGPDKIRV 180 SAKTFVPYFRQVGGG F+NIASTAGIRPRPGLTWYNGSKGAVITTSKSMAAELGPDKIRV Sbjct: 121 SAKTFVPYFRQVGGGAFINIASTAGIRPRPGLTWYNGSKGAVITTSKSMAAELGPDKIRV 180 Query: 181 NCVNPVISATGLLAEFMGVPDTPENRKKFVATIPMGRFSTPEDIANACLYLGSDEAQFVT 240 NCVNPVISATGLL+EFMGVPDTPENRKKFVATIPMGRFSTPEDIANACLYLGSDEA+FVT Sbjct: 181 NCVNPVISATGLLSEFMGVPDTPENRKKFVATIPMGRFSTPEDIANACLYLGSDEAEFVT 240 Query: 241 GVCIEVDGGRCV 252 GVCIEVDGGRCV Sbjct: 241 GVCIEVDGGRCV 252 >gi|300313109|ref|YP_003777201.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 266 Score = 144 bits (364), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 95/264 (35%), Positives = 142/264 (53%), Gaps = 19/264 (7%) Query: 3 LQDKVAIVTGGGS---GFGEGIAKA--YAREGAAVVVADIGQTGGLRVVEEIQAGGGKAI 57 +Q K IVTG GS G+G G A A YAREG V+ D L+ + I++ GG Sbjct: 5 MQGKAIIVTGAGSVGEGWGNGKAAAVLYAREGGRVLAVDRSIEAALQTQDIIRSEGGVCE 64 Query: 58 FAKADVSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRPMLEVEEEEFDRVYAVNVKS 117 ADVS+ ++ + A++HFG++D++ NN G P +E EE ++R+ AVN S Sbjct: 65 VLAADVSRNTEVKAIAEAAMDHFGRVDVLHNNVGIADVGGP-VETSEESWNRLVAVNQTS 123 Query: 118 IFLSAKTFVPYFRQVGGGTFVNIASTAGIR----PRPGLTWYNGSKGAVITTSKSMAAEL 173 +FL+ K +P + G VNI+S A +R P G Y +K A++ +K++A + Sbjct: 124 LFLTHKHVLPIMEKQRKGAIVNISSLAALRWIGFPYLG---YTATKAAILAFTKNVAMQY 180 Query: 174 GPDKIRVNCVNPVISATGLLAEFMGVP---DTPENRKKFVATIPMGRFSTPEDIANACLY 230 P IR NCV P + T ++ E + D + R K A PMG D+A+A L+ Sbjct: 181 APMGIRANCVLPGMMDTPMIREPLKASYGGDIEQMRAKRNAQCPMGFMGDAWDVAHASLF 240 Query: 231 LGSDEAQFVTGVCIEVDGG---RC 251 L SD A+++TG+ + VDGG RC Sbjct: 241 LASDHARYITGLDMIVDGGLSLRC 264 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 142 bits (358), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 89/248 (35%), Positives = 135/248 (54%), Gaps = 7/248 (2%) Query: 2 RLQDKVAIVTGGGSGFGEGIAKAYAREGAAVVVADIGQTGGLRVVEEIQAGGGKAIFAKA 61 R + KV IVTG SG GE A+ ++ EGA V++AD +V + + A + Sbjct: 11 RFEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPPERTAA--RET 68 Query: 62 DVSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRPMLEVEEEEFDRVYAVNVKSIFLS 121 DVS + QL+ A+E FG+LD++V++AG + EV E++ RV A NV +F Sbjct: 69 DVSHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGN-VTEVSPEDWHRVQATNVNGVFYG 127 Query: 122 AKTFVPYFRQVGGGTFVNIASTAGIRPRPGLTWYNGSKGAVITTSKSMAAELGPDKIRVN 181 A+ +P+ + G VN+AS +G+ L+ YN SKGAV +++MA + G +R+N Sbjct: 128 AREALPHLEKTRG-CIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKGVRIN 186 Query: 182 CVNPVISATGLLAEFMGVPDTPENRKKFVATIPMGRFSTPEDIANACLYLGSDEAQFVTG 241 V P ++ T + A+ D P KF I +GR + P +IA +L S +A FV G Sbjct: 187 AVCPSLTHTAMTADMA---DDPPLLDKFAERIALGRGADPLEIAAVITFLASPDASFVNG 243 Query: 242 VCIEVDGG 249 V + VDGG Sbjct: 244 VNLPVDGG 251 >gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 139 bits (351), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 90/249 (36%), Positives = 136/249 (54%), Gaps = 8/249 (3%) Query: 2 RLQDKVAIVTGGGSGFGEGIAKAYAREGAAVVVADIGQTGGLRVVEEIQAGGGKAIFAKA 61 R +DKV +VTGG G G AK +A EGA V V+ + V EI G A+ Sbjct: 6 RFEDKVVVVTGGSEGIGLATAKLFAAEGARVYVSGRRADKLEQAVREI---GNGAVGVPG 62 Query: 62 DVSKRADMDQLLATALEHFGKLDIVVNNAGT-THRNRPMLEVEEEEFDRVYAVNVKSIFL 120 D +K D+D+L A G+LD++ NAGT + +P+ + EE+FD ++ +NV+ + Sbjct: 63 DAAKLDDLDRLYARVQADHGRLDVLFANAGTASTEAKPLGMIGEEDFDGLFNLNVRGLLF 122 Query: 121 SAKTFVPYFRQVGGGTFVNIASTAGIRPRPGLTWYNGSKGAVITTSKSMAAELGPDKIRV 180 S + +P Q GG V S AG++ PG + YN SK AV + ++S A+L IRV Sbjct: 123 SVQKALPLMSQ--GGAIVLNGSVAGVKGFPGQSLYNASKAAVRSFARSWTADLKERGIRV 180 Query: 181 NCVNPVISATGLLAEFMGVPDTPENRKKFVATIPMGRFSTPEDIANACLYLGSDEAQFVT 240 N V P + T L+ +++ E+ K V +P+GR P++I A L+L S E+ ++ Sbjct: 181 NVVAPGGTETRLMRDYLEARPGMEDVLKQV--VPLGRLGEPDEIGRAVLFLASAESSYIA 238 Query: 241 GVCIEVDGG 249 GV + VDGG Sbjct: 239 GVELFVDGG 247 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 139 bits (350), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 85/259 (32%), Positives = 141/259 (54%), Gaps = 18/259 (6%) Query: 1 MRLQDKVAIVTGGG--SGFGEGIAKAYAREGAAVVVADIGQTGGLRVVEEIQAGGGKAIF 58 M L+DKVA++TGG +G G A+ A +GA VVV D+ Q I G + + Sbjct: 1 MLLKDKVAVITGGAGINGLGYATARLMAEQGARVVVLDLAQADPAAAAARI---GSQHLG 57 Query: 59 AKADVSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRPMLEVEEEEFDRVYAVNVKSI 118 ADV+ +A + A L+ FG++DI+VNNAG T + LE+ ++DR+ V+++ Sbjct: 58 LVADVTDKASCETAAAAILQQFGRIDILVNNAGITQPVKT-LEITGADYDRILDVSLRGT 116 Query: 119 FLSAKTFVPYFRQVGGGTFVNIASTA-----GIRPRPGLTWYNGSKGAVITTSKSMAAEL 173 ++ +P R G+ + I+S + GI P Y+ +K V+ +++MA E Sbjct: 117 LYMSQAVLPAMRAQQNGSIICISSVSAQRGGGILGGP---HYSAAKAGVLGLARAMAREF 173 Query: 174 GPDKIRVNCVNPVISATGLLAEFMGVPDTPENRKKFVATIPMGRFSTPEDIANACLYLGS 233 GP+ IRVN + P + T ++ + T E + + TIP+ R +DI AC++LGS Sbjct: 174 GPEGIRVNSITPGLIGTDIIKGKL----TEEKKAEIAETIPLARLGRADDIGGACVFLGS 229 Query: 234 DEAQFVTGVCIEVDGGRCV 252 D +Q+ TG+ ++V+GG + Sbjct: 230 DLSQYCTGITLDVNGGMLI 248 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 823,797 Number of extensions: 35688 Number of successful extensions: 276 Number of sequences better than 1.0e-08: 51 Number of HSP's gapped: 167 Number of HSP's successfully gapped: 51 Length of query: 252 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 169 Effective length of database: 1,226,865 Effective search space: 207340185 Effective search space used: 207340185 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)