BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2104.p (435 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 822 0.0 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 508 e-145 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 421 e-119 gi|300313342|ref|YP_003777434.1| major facilitator superfamily p... 378 e-106 gi|300313738|ref|YP_003777830.1| major facilitator superfamily p... 359 e-100 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 822 bits (2124), Expect = 0.0, Method: Compositional matrix adjust. Identities = 422/435 (97%), Positives = 430/435 (98%) Query: 1 MTYQAGAAADSARASFEDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFS 60 M+YQAGAAAD ARASFEDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFS Sbjct: 1 MSYQAGAAADGARASFEDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFS 60 Query: 61 DTVYGLGAGIFFLGYFVFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYI 120 DTVYGLGAGIFFLGYFVFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYI Sbjct: 61 DTVYGLGAGIFFLGYFVFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYI 120 Query: 121 MRFLLGVAEAGFFPGVILYLTYWYPAARRGKITALFMTGIALSGVIGGPFSGWIMHAMPG 180 MRFLLGVAEAGFFPGVILYLTYWYPAARRGKITALFMTGIALSGVIGGP SGWIMHAMPG Sbjct: 121 MRFLLGVAEAGFFPGVILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPG 180 Query: 181 VWGHTGWQWMFILEAIPSLVLGVLVILYMKDRIRDAEWLSEDEKRLLEANISHEESQKEH 240 VWGHTGWQWMFILEAIPSL+LGV+VILYMKDRIRDA+WLSE+EKRLLEANISHEESQKEH Sbjct: 181 VWGHTGWQWMFILEAIPSLILGVMVILYMKDRIRDADWLSEEEKRLLEANISHEESQKEH 240 Query: 241 LSLGAMFANGRVWLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAA 300 LSLGAMFANGRVWLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAA Sbjct: 241 LSLGAMFANGRVWLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAA 300 Query: 301 AAMVLIGRSADARRERRWHVAIPAFIGCIGLLLSTLYGHNTALAMASLTLASVGIMTTLP 360 AAMVLIGRSADARRERRWHVAIPAFIGCIGLLLST YGHNT LAMASLTLASVGI+TTLP Sbjct: 301 AAMVLIGRSADARRERRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLP 360 Query: 361 LFWSLPTAFLSGTAAAAGIALINSLGNLAGFVSPFLVGWLKDTTQSTNAGMYVLAASLVI 420 LFWSLPTAFLSGTAAAAGIALINSLGNLAGFVSPFLVGWLKD TQSTNAGMYVLAASLVI Sbjct: 361 LFWSLPTAFLSGTAAAAGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAASLVI 420 Query: 421 GGLLTLTLPARLVNK 435 GGLLTLTLPA+LVN+ Sbjct: 421 GGLLTLTLPAKLVNR 435 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 508 bits (1309), Expect = e-145, Method: Compositional matrix adjust. Identities = 247/420 (58%), Positives = 325/420 (77%), Gaps = 1/420 (0%) Query: 17 EDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYF 76 +D Y KV WR++P L LCYV +YLDRVNVGFAKLQM QDL FS+TV+GLGAG+FF+GYF Sbjct: 18 KDGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLFFIGYF 77 Query: 77 VFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGV 136 +FEVPSN+++HKVGAR+WIARIMITWGI+S A +V FYI+RFLLG+AEAGF+PG+ Sbjct: 78 LFEVPSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYILRFLLGLAEAGFYPGI 137 Query: 137 ILYLTYWYPAARRGKITALFMTGIALSGVIGGPFSGWIMHAMPGVWGHTGWQWMFILEAI 196 ILYLTYW+P+ RR K+ A+FM+GI ++G++G P SGWIM A G GWQWMF++EAI Sbjct: 138 ILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMFLIEAI 197 Query: 197 PSLVLGVLVILYMKDRIRDAEWLSEDEKRLLEANISHEESQKE-HLSLGAMFANGRVWLG 255 P++++GV +LY+ + ++ A+WL+++EK L+A+I + KE GA+ + RVWL Sbjct: 198 PAVLIGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKESKHGFGAIVKDARVWLM 257 Query: 256 ALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADARRE 315 LIYF FVMG YG++ W+PT++KATGV L +GLL+AIP+ A AM LIGRSAD RE Sbjct: 258 CLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIGRSADRMRE 317 Query: 316 RRWHVAIPAFIGCIGLLLSTLYGHNTALAMASLTLASVGIMTTLPLFWSLPTAFLSGTAA 375 RRWH+ IPA +G +G + + L+ NTA+++ASL+LA+ G++T PLFWSLPTAFLSG AA Sbjct: 318 RRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAPLFWSLPTAFLSGAAA 377 Query: 376 AAGIALINSLGNLAGFVSPFLVGWLKDTTQSTNAGMYVLAASLVIGGLLTLTLPARLVNK 435 A GIA INS+GNLAGFVSP+L+G+LKD T + GMY+LA LV+G + TL +VNK Sbjct: 378 AVGIAAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYMLAVMLVVGSIATLATKPGMVNK 437 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 421 bits (1082), Expect = e-119, Method: Compositional matrix adjust. Identities = 218/412 (52%), Positives = 291/412 (70%), Gaps = 1/412 (0%) Query: 18 DATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYFV 77 + TY K+ WRL+P L +V +++DRVNVGFAKLQMLQDL+FS+ VYGLGAGIFF+GYF+ Sbjct: 22 EVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFI 81 Query: 78 FEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGVI 137 FEVPSN++L K+GAR +ARI I WG+ S AMAYVTTP FYI+RFLLG+ EAGFFPGV+ Sbjct: 82 FEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFPGVV 141 Query: 138 LYLTYWYPAARRGKITALFMTGIALSGVIGGPFSGWIMHAMPGVWGHTGWQWMFILEAIP 197 LYLTYW+PA RR ++ LFMT A++G +GGP +G IM M GV WQW+FILE IP Sbjct: 142 LYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMSGMEGVGHLANWQWLFILEGIP 201 Query: 198 SLVLGVLVILYMKDRIRDAEWLSEDEKRLLEANISHEESQK-EHLSLGAMFANGRVWLGA 256 S++ G V+ ++ +R +A+WLSE EK + ++ E S +H S N RVW+ A Sbjct: 202 SILAGFAVLAWLPERPANAKWLSEAEKNAVTQVLAAENSAPGKHFSFKDACTNYRVWICA 261 Query: 257 LIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADARRER 316 +YFC V G ++FW P+IIK GV L +GL++AIP+ AM+ G +D ER Sbjct: 262 AVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGFHSDKTGER 321 Query: 317 RWHVAIPAFIGCIGLLLSTLYGHNTALAMASLTLASVGIMTTLPLFWSLPTAFLSGTAAA 376 R H AI I CIGL+ + + N LA+ SLTLA++GI+ P+FWS+P AFL+GTAAA Sbjct: 322 RLHSAIATLIACIGLVATGMTLGNATLALCSLTLAAIGILAAFPVFWSIPGAFLAGTAAA 381 Query: 377 AGIALINSLGNLAGFVSPFLVGWLKDTTQSTNAGMYVLAASLVIGGLLTLTL 428 GIALINS+GNLAGFV+P+++G LK +T S ++G+Y +AA ++ L + Sbjct: 382 GGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALELVASFLVVVF 433 >gi|300313342|ref|YP_003777434.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 378 bits (971), Expect = e-106, Method: Compositional matrix adjust. Identities = 202/428 (47%), Positives = 286/428 (66%), Gaps = 11/428 (2%) Query: 7 AAADSARASFEDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGL 66 A A A D Y KVTW++LP L L YV +YLDRVNVGFAKL ML DL S+ YGL Sbjct: 2 ANASQGSAVQFDGLYKKVTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAYGL 61 Query: 67 GAGIFFLGYFVFEVPSNMILHKVGARLWIARIMITWGIISGAMAY---------VTTPEM 117 GAGIFFLGYF+FEVPSN+ILH+VGA++WIARIMITWG+IS MA+ V+ + Sbjct: 62 GAGIFFLGYFIFEVPSNLILHRVGAKIWIARIMITWGMISSLMAFTAPIAQWLGVSNETV 121 Query: 118 FYIMRFLLGVAEAGFFPGVILYLTYWYPAARRGKITALFMTGIALSGVIGGPFSGWIMHA 177 FY +RFLLG +EAGFFPGVILY+TYWYPAA ++ A+ + G S ++GGP SG +M Sbjct: 122 FYTLRFLLGASEAGFFPGVILYMTYWYPAAMHSRVMAVVLLGNPFSYILGGPISGALMQF 181 Query: 178 MPGVWGHTGWQWMFILEAIPSLVLGVLVILYMKDRIRDAEWLSEDEKRLLEANISHEESQ 237 M GWQWMF++EA+P+ +LG++++ + + I A+WL+ +EK L ++ E + Sbjct: 182 MGSGDSLAGWQWMFVIEALPACLLGIVILFVLSNDIASAKWLAPEEKAELARRLAAENKE 241 Query: 238 KEHLSLGAMFANGRVWLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYA 297 K ++ L +++A +W AL+Y ++G YG++FW PTII+ GV L++G+++AIPY Sbjct: 242 KVNVPLKSLYALPILWFLALVYLLMMIGSYGINFWAPTIIRDAGVKSVLDIGMISAIPYI 301 Query: 298 FAAAAMVLIGRSAD-ARRERRWHVAIPAFIGCIGLLLSTLYGHNTALAMASLTLASVGIM 356 A AMV I R A+ A + R + VA+ + + + L+L TL+ HN + +A + +A G + Sbjct: 302 LGATAMVFITRHAEKAGKHRSYSVAV-SMVAGLALVLCTLFPHNIYITLAGIAIAVGGSL 360 Query: 357 TTLPLFWSLPTAFLSGTAAAAGIALINSLGNLAGFVSPFLVGWLKDTTQSTNAGMYVLAA 416 T++ LFW P A+ +G AAAGIA+INS+GNL GFV P+L+G L S G+ +L Sbjct: 361 TSIALFWGFPGAYFTGATAAAGIAIINSVGNLGGFVGPYLLGALTTAFGSPRYGLMMLGG 420 Query: 417 SLVIGGLL 424 +L + G+L Sbjct: 421 ALFLAGVL 428 >gi|300313738|ref|YP_003777830.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 359 bits (921), Expect = e-100, Method: Compositional matrix adjust. Identities = 191/421 (45%), Positives = 273/421 (64%), Gaps = 8/421 (1%) Query: 17 EDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYF 76 EDAT KV RL+P + LCYV SYLDR+NVGFA L M +DL + + +GLGAG+FF+GYF Sbjct: 20 EDATMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKDLGLTPSQFGLGAGLFFIGYF 79 Query: 77 VFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGV 136 FE+PSN+ LH+ GAR+WI+RIMI+WG+IS A A+V P+ F + RFLLG+AEAGF PG+ Sbjct: 80 FFEIPSNLALHRFGARMWISRIMISWGLISMATAFVVGPKSFALARFLLGMAEAGFTPGI 139 Query: 137 ILYLTYWYPAARRGKITALFMTGIALSGVIGGPFSGWIMHAMPGVWGHTGWQWMFILEAI 196 LY T W+P A RGK TA F+ GI ++ +IG P SG +M + G+WG GWQ + +LEA+ Sbjct: 140 YLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALME-LHGMWGFKGWQVLLLLEAL 198 Query: 197 PSLVLGVLVILYMKDRIRDAEWLSEDEKRLLEANISHEES---QKEHLSLGAMFANGRVW 253 P+++LGV+ + + DR A+WLS DEK+ LE +S E++ + L F N RV+ Sbjct: 199 PAVLLGVMCLFLLPDRPAKAKWLSADEKQWLENELSTEQNVLAARHGNKLKDAFTNWRVF 258 Query: 254 LGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADAR 313 A FC ++G + WLP II+ G+ VGL+ AIPY A AM L R A+ Sbjct: 259 ALAGANFCGIIGSLSIGLWLPQIIREFGLPSH-QVGLVAAIPYLVGAVAMTLWARLANRS 317 Query: 314 RERRWHVAIPAFIGCIGLLLSTLYGHNTALAMASLTLASVGIMTTLPLFWSLPTAFLSGT 373 R + VA + + L +S H L M ++T+A I++ FW++P+ FL+G Sbjct: 318 ERRLFFVAGAIVLAALSLGISAFL-HTPLLKMLAITVAVASILSFQATFWAIPSGFLTGR 376 Query: 374 AAAAGIALINSLGNLAGFVSPFLVGWLKDTTQSTNAGMYVLAASLVIGGLLTLTL--PAR 431 AAA G+ALI S+GNL GFV P ++G++++ TQ + +A +L++G ++TL L P+R Sbjct: 377 AAAGGLALIVSIGNLGGFVGPSVIGFIREATQGFTYPLIFVAGALLLGAVITLALGDPSR 436 Query: 432 L 432 + Sbjct: 437 V 437 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.140 0.442 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,390,210 Number of extensions: 61441 Number of successful extensions: 379 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 341 Number of HSP's successfully gapped: 20 Length of query: 435 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 347 Effective length of database: 1,203,190 Effective search space: 417506930 Effective search space used: 417506930 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)