BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2077.p (553 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311543|ref|YP_003775635.1| major facilitator superfamily p... 1034 0.0 gi|300312656|ref|YP_003776748.1| alpha-ketoglutarate permease of... 194 1e-50 gi|300313056|ref|YP_003777148.1| major facilitator superfamily p... 186 4e-48 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 179 4e-46 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 179 7e-46 >gi|300311543|ref|YP_003775635.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 553 Score = 1034 bits (2673), Expect = 0.0, Method: Compositional matrix adjust. Identities = 540/553 (97%), Positives = 549/553 (99%) Query: 1 MTTASVNTTTRGITKEEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFI 60 MTTASVNTT RGIT EEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFI Sbjct: 1 MTTASVNTTARGITPEEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFI 60 Query: 61 FALLAFAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITA 120 FALLAFAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITA Sbjct: 61 FALLAFAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITA 120 Query: 121 PIILIILRILQGLALGGEYGGAATYVAEHAPEGRRGSFTAWIQTTATMGLFLSLLVILGV 180 PIILIILRILQGLALGGEYGGAATYVAEHAPEGRRGSFTAWIQTTATMGLFLSLLVILGV Sbjct: 121 PIILIILRILQGLALGGEYGGAATYVAEHAPEGRRGSFTAWIQTTATMGLFLSLLVILGV 180 Query: 181 RTSVGEAAFADWGWRIPFLVSILLLAISVWIRMSMNESPAFLKMKAEGKTSKAPLTEAFG 240 RT+VGEAAFADWGWRIPFLVSILLLAISVWIRMSMNESPAFLKMKAEGKTSKAPLTEAFG Sbjct: 181 RTAVGEAAFADWGWRIPFLVSILLLAISVWIRMSMNESPAFLKMKAEGKTSKAPLTEAFG 240 Query: 241 QWKNLKLVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGPTANLLIAAALLLATPFF 300 QWKNLKLVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGPTAN+LIAAALLLATPFF Sbjct: 241 QWKNLKLVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGPTANILIAAALLLATPFF 300 Query: 301 LFFGSLSDKIGRKPIILGGCLIAAVTYFPIFNGLTHFANPALEAALQKSPVIVTADPASC 360 LFFGSLSDKIGRKPIILGGCLIAAVTYFPIF+GLTHFANPALEAALQKSPVIVTADPASC Sbjct: 301 LFFGSLSDKIGRKPIILGGCLIAAVTYFPIFSGLTHFANPALEAALQKSPVIVTADPASC 360 Query: 361 QFQFNPTGTKKFTSSCDIVKAKLSAASVNYQNVAGAPGSVATVKIGERIITSYDATGLSK 420 QFQFNPTGTKKFTSSCDIVKAKLSAASVNYQNVAGAPGSVATVKIG+RIITSYDA GLSK Sbjct: 361 QFQFNPTGTKKFTSSCDIVKAKLSAASVNYQNVAGAPGSVATVKIGDRIITSYDAAGLSK 420 Query: 421 EEAAAKDKELSKALADDIKAAGYPAKADPEQINKPMVLLLLFILVIYVTMVYGPIAACLV 480 E+AAAKDK+LSK LADDIKAAGYP+KADPEQ+NKPMVLLLLFILVIYVTMVYGPIAACLV Sbjct: 421 EDAAAKDKQLSKDLADDIKAAGYPSKADPEQMNKPMVLLLLFILVIYVTMVYGPIAACLV 480 Query: 481 EMFPTRIRYTSMSMPYHIGNGWFGGLLPTTSFALVAFKGDIYYGLWYPIVIALITFVIGM 540 EMFPTRIRYTSMSMPYHIGNGWFGGLLPTTSFALVAFKGDIYYGLWYPIVIA+ITFVIGM Sbjct: 481 EMFPTRIRYTSMSMPYHIGNGWFGGLLPTTSFALVAFKGDIYYGLWYPIVIAMITFVIGM 540 Query: 541 LFVKETVRNNIHD 553 LFVKETVRNNIHD Sbjct: 541 LFVKETVRNNIHD 553 >gi|300312656|ref|YP_003776748.1| alpha-ketoglutarate permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 194 bits (494), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 122/335 (36%), Positives = 189/335 (56%), Gaps = 12/335 (3%) Query: 16 EEKKVIFA---SSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFIFALLAFAAGFIV 72 E +K IFA +S G + EW+DFY+Y A A FF DP + + FAAGF++ Sbjct: 15 EYRKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDPTSQLLNTAGVFAAGFLM 74 Query: 73 RPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITAPIILIILRILQG 132 RP G +FGR+ D GRK + L+++L+M + V V+P Y +IG AP +L++ R+ QG Sbjct: 75 RPIGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVAVMPTYATIGAWAPALLLLARLFQG 134 Query: 133 LALGGEYGGAATYVAEHAPEGRRGSFTAWIQTTATMGLFLSLLVILGVRTSVGEAAFADW 192 L++GGEYG +ATY++E AP GRRG F ++ T G L++LV+ G++ + +A W Sbjct: 135 LSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLFGMQQWLTKAELMAW 194 Query: 193 GWRIPFLVSILLLAISVWIRMSMNE-SPAFLKMKAEGKTSKAPLTEAFGQWKNLKLVILA 251 GWR+PF++ + +++++R S+ E S A + K + T K L K L Sbjct: 195 GWRVPFVLGAVGALVAMYLRSSLAETSSAGARKKKDAGTLKGLLQH--------KRAFLN 246 Query: 252 LVGLTAGQAVVWYTGQFYALFFLTQTLKVDGPTANLLIAAALLLATPFFLFFGSLSDKIG 311 +VG TAG ++++YT Y +L T +D AN ++ AL + FG++SDKIG Sbjct: 247 VVGFTAGGSLMFYTFTTYMQKYLVNTAGMDPKVANGVMTGALFVYMILQPIFGAISDKIG 306 Query: 312 RKPIILGGCLIAAVTYFPIFNGLTHFANPALEAAL 346 R+ +L V FPI + L ++P + AL Sbjct: 307 RRNSMLCFAFFGMVGTFPILHFLKDVSSPGVAMAL 341 >gi|300313056|ref|YP_003777148.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 465 Score = 186 bits (473), Expect = 4e-48, Method: Compositional matrix adjust. Identities = 113/330 (34%), Positives = 182/330 (55%), Gaps = 16/330 (4%) Query: 7 NTTTRGITKEEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQ-FFAGTDPNTAFIFALLA 65 +T + ++ KK + +G+ E+YDF++Y + A++I Q FF DP TA I +L Sbjct: 3 STQSNAHHQQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLAT 62 Query: 66 FAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITAPIILI 125 + G++ RP GA V G LGD GRK L + +MG ST VG+LP Y+ +G+ AP +L+ Sbjct: 63 YGVGYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLV 122 Query: 126 ILRILQGLALGGEYGGAATYVAEHAPEGRRGSFTAWIQTTATMGLFLSLLVILGVRTSVG 185 I+R+ QG A+ GE GA++ + EH+P GRRG F+++ G L+ V L + + Sbjct: 123 IMRLAQGFAVAGEISGASSMILEHSPFGRRGYFSSFTLQGVQAGQILAAAVFLPLAHYMP 182 Query: 186 EAAFADWGWRIPFLVSILLLAISVWIRMSMNESPAFLKMKAEGKTSKAPLTEAFGQWKN- 244 F WGWRIPFL+S L++ IR +++E+P F E ++P+ +AF Sbjct: 183 AEQFNVWGWRIPFLLSFLVIIAGWVIRRTVDETPVFKAADKEELKRRSPVVDAFRDCSPN 242 Query: 245 -LKLVILALVGLTAGQAVVWYTGQFYAL-----FFLTQTLKVDGPTANLLIAAALLLATP 298 L++V +AL+ + A ++ G YA+ + + + P ++A +L P Sbjct: 243 MLRVVCMALMNVIPVVATIF--GAAYAVQPAYGIGFAKDVYLWIPVVGNIVA---VLVIP 297 Query: 299 FFLFFGSLSDKIGRKPIILGGCLIAAVTYF 328 + G LSD+IGR+P I+ G L+A + F Sbjct: 298 ---YVGKLSDRIGRRPPIIVGSLLAGLFAF 324 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 179 bits (455), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 106/324 (32%), Positives = 177/324 (54%), Gaps = 20/324 (6%) Query: 15 KEEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFIFALLAFAAGFIVRP 74 ++ ++ I A+ +G EW+DF +Y A IIAK FF + T+ + A+ F GF +RP Sbjct: 17 QQRRRAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVGFFMRP 76 Query: 75 FGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITAPIILIILRILQGLA 134 G ++ G D VGRK VTIL+M T ++G+ P Y SIG+ AP+I+++ R+LQG + Sbjct: 77 VGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLLQGFS 136 Query: 135 LGGEYGGAATYVAEHAPEGRRGSFTAWIQTTATMGLFLSLLVILGVRTSVGEAAFADWGW 194 GGE G A ++ E+APE R +++WIQ + + + L V V S+ AA A WGW Sbjct: 137 AGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALASWGW 196 Query: 195 RIPFLVSILLLAISVWIRMSMNESPAFLKMKAEGKTSKAPLTEAFGQWKNLKLVILALVG 254 R+PFL+ I++ + +IR + E+P FL+ E + +PL E + ++V Sbjct: 197 RLPFLLGIIIGPVGYYIRHHLEETPTFLE---EKDKTDSPLKEVIRNFPRETSASFSMV- 252 Query: 255 LTAGQAVVWYTGQFYALFFL-TQTLKV------DGPTANLLIAAALLLATPFFLFFGSLS 307 ++W + LF++ T ++KV G A ++ +++ P G L+ Sbjct: 253 ------ILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAP---IVGRLA 303 Query: 308 DKIGRKPIILGGCLIAAVTYFPIF 331 D+IGR+P++ G + + +P+F Sbjct: 304 DRIGRRPLLSGSAALILMLAYPMF 327 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 179 bits (453), Expect = 7e-46, Method: Compositional matrix adjust. Identities = 108/338 (31%), Positives = 179/338 (52%), Gaps = 17/338 (5%) Query: 5 SVNTTTRG--ITK-EEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFIF 61 S NT G IT + ++ I +SS+G EW+D +YG+ A +IAKQFF D + + + Sbjct: 2 SSNTAAPGADITHPQARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLL 61 Query: 62 ALLAFAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITAP 121 F F +RP GA+V G D GRK +++I++M T ++ +P+Y SIG+ AP Sbjct: 62 TFATFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAP 121 Query: 122 IILIILRILQGLALGGEYGGAATYVAEHAPEGRRGSFTAWIQTTATMGLFLSLLVILGVR 181 + + +++QG + GGE+G + ++ EHAP RRG F++W + + L L+ L + Sbjct: 122 AGIALGKMIQGFSAGGEFGSSTAFLVEHAPH-RRGFFSSWQVASQGISLLLAALFGAVLN 180 Query: 182 TSVGEAAFADWGWRIPFLVSILLLAISVWIRMSMNESPAFLKMKAEGKTSKAPLTEAFGQ 241 + A WGWR+PF+ +L+ ++IR ++E+P F K + + APL + F Sbjct: 181 NMLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEF---KESSEKTNAPLRDTFAH 237 Query: 242 WKNLKLVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGPTANLLIAAALLLATPFFL 301 K ++L+I AG ++ + +L+ T +K G A AA + F+ Sbjct: 238 QK-MRLLI------GAGSVIMATVSVYLSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFI 290 Query: 302 ---FFGSLSDKIGRKPIILGGCLIAAVTYFPIFNGLTH 336 G+LSDKIGR P ++ + V +P+F LT+ Sbjct: 291 GSPLVGALSDKIGRTPFMITSSALYIVLTYPMFVFLTN 328 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.140 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,463,495 Number of extensions: 53046 Number of successful extensions: 336 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 310 Number of HSP's successfully gapped: 17 Length of query: 553 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 463 Effective length of database: 1,193,720 Effective search space: 552692360 Effective search space used: 552692360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)