BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2070.p (753 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311534|ref|YP_003775626.1| lipoprotein transmembrane prote... 1295 0.0 gi|300310707|ref|YP_003774799.1| esterase of the alpha-beta hydr... 140 4e-34 >gi|300311534|ref|YP_003775626.1| lipoprotein transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 758 Score = 1295 bits (3351), Expect = 0.0, Method: Compositional matrix adjust. Identities = 634/718 (88%), Positives = 675/718 (94%) Query: 36 RPRVCLVLSGGGARGYAHLGVLQYLEQLHIPIDCIAGTSMGALIGGLYASGLSAEELEHR 95 RPRVCLVLSGGGARGYAHLGVLQYLEQLHIPIDCIAGTSMGALIGGLYASGL A ELE R Sbjct: 41 RPRVCLVLSGGGARGYAHLGVLQYLEQLHIPIDCIAGTSMGALIGGLYASGLDAGELERR 100 Query: 96 LAVTNLSDIAFDRTERSRLPQSQREDDFQYPISLSAGLDDGKLKLASGLVQGNNLLALLQ 155 LA TNLSDIAFDR ERSRLPQ+QRED+FQYPIS+SAGLD KLKLASGLVQGN LLALLQ Sbjct: 101 LAATNLSDIAFDRNERSRLPQAQREDEFQYPISISAGLDGSKLKLASGLVQGNKLLALLQ 160 Query: 156 NWTAQLPANIDFARLPIPFRAVATDLSLGTEVVLDHGSLPRAMRASMAVPGLFAPFSIDQ 215 NWTAQLPANIDFARLPIPFRAVATDLSLG+EVVLDHGSLPRAMRASMAVPGLFAPF IDQ Sbjct: 161 NWTAQLPANIDFARLPIPFRAVATDLSLGSEVVLDHGSLPRAMRASMAVPGLFAPFRIDQ 220 Query: 216 RTLVDGGLVSNLPVQTARDMGADVVIAVNIATPLQDAAQLQSPTAVAQQMVGILIQQNVK 275 RTLVDGGLVSNLPVQTARDMGADVVIAVNIATPLQDAAQLQSPTAVAQQMVGILIQQNVK Sbjct: 221 RTLVDGGLVSNLPVQTARDMGADVVIAVNIATPLQDAAQLQSPTAVAQQMVGILIQQNVK 280 Query: 276 AQKALLGAQDILIEPQMTGMSFTDFARGKDGINAGWDAAQRQNARLLQLSLTPQQWQAYL 335 AQKALLGAQDILIEPQM GMSFTDFARGKDG+NAGW+AAQRQ ARLLQLSLTP QWQAYL Sbjct: 281 AQKALLGAQDILIEPQMEGMSFTDFARGKDGVNAGWEAAQRQQARLLQLSLTPTQWQAYL 340 Query: 336 QTRSHSSLALAADTRIDAIEIRTGGHIPASYVRSRLDVKEGDRYDGQQINQELTRLSTHG 395 R+H +ALAA TRIDAIEIR+G IPASYVRSRL VKEGD YDGQ+INQ+L++LST+G Sbjct: 341 DRRAHDGMALAAGTRIDAIEIRSGSRIPASYVRSRLAVKEGDVYDGQRINQQLSQLSTNG 400 Query: 396 DFNNVTQELVTRPDGRHVLVIDAEEKSWGPQYLLFGLGISNSFNGQGGFNLQIGHRYPWI 455 DFNNVTQELV+R DGRHVL+IDAEEKSWGPQYLLFGLG+SNSFNG+GGFNLQ+GHRYPW+ Sbjct: 401 DFNNVTQELVSREDGRHVLLIDAEEKSWGPQYLLFGLGVSNSFNGRGGFNLQLGHRYPWM 460 Query: 456 NDSGLEWRNDLVLGNKRASLQSELRQPIWGSYGAYLAPYLELSRRYVDIYLDGSDVKASP 515 N SGLEWRNDLVLG KRASLQ+ELRQPIWGS GAYL+PYLE+SRRYVD+Y DGSDVKASP Sbjct: 461 NQSGLEWRNDLVLGAKRASLQTELRQPIWGSTGAYLSPYLEVSRRYVDLYPDGSDVKASP 520 Query: 516 VNQYRIDSAIGGMDLGLPLSRLGELRLGMSYQHVKYSPTYNLPPNSGLQFASYQSSQPVA 575 +N+YRIDSA GG+DLGLPLSRLGELRLG+SYQHV YSPTYNLP GL F S Q+SQPVA Sbjct: 521 INEYRIDSAQGGLDLGLPLSRLGELRLGLSYQHVNYSPTYNLPAGGGLLFESVQTSQPVA 580 Query: 576 RMRLTIDQLDDVLFPRQGYYLSAEANRGFGSQERRFSNAQARTLWAVSRDRNTLNLALEA 635 R+RLTIDQLDD LFPR+GYYLSAEANRG GS E RFSN QARTLWAVSR+RNTLN+ALE Sbjct: 581 RLRLTIDQLDDALFPRKGYYLSAEANRGLGSDEHRFSNVQARTLWAVSRERNTLNVALEG 640 Query: 636 ASSLSANSAGLGFTLGGFQHLSAYAPEQFFGNYLLYGRLTWLRDLADYSLPGLRKPVLGA 695 ASSLSANSAG+GFTLGGFQHLSAYAP+QFFGNYLLYGRL+WLRDL D SLPGLR PVLGA Sbjct: 641 ASSLSANSAGIGFTLGGFQHLSAYAPDQFFGNYLLYGRLSWLRDLVDTSLPGLRNPVLGA 700 Query: 696 SLEAGNVWQSRDAFGDGPYKKSMSLFLGGTSPIGPLYFGVALGQQGVWNVYLQLGRVF 753 SLEAGNVWQ+RDAFGDGPYKKS+SLFLGG+SP+GPLYFGVALGQQGVWN+YLQLGRVF Sbjct: 701 SLEAGNVWQNRDAFGDGPYKKSLSLFLGGSSPVGPLYFGVALGQQGVWNLYLQLGRVF 758 >gi|300310707|ref|YP_003774799.1| esterase of the alpha-beta hydrolase superfamily protein [Herbaspirillum seropedicae SmR1] Length = 316 Score = 140 bits (352), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 101/280 (36%), Positives = 143/280 (51%), Gaps = 33/280 (11%) Query: 35 HRPRVCLVLSGGGARGYAHLGVLQYLEQLHIPIDCIAGTSMGALIGGLYASGLSAEELEH 94 R ++ L L GG ARG+AH+GV++ LE + +D + GTS G+L+G LYA+G L+ Sbjct: 52 RRLKIGLALGGGAARGFAHIGVIKALEAQGVQVDVVTGTSAGSLVGALYAAGNDGFALQ- 110 Query: 95 RLAVTNLSDIAFDRTERSRLPQSQREDDFQYPISLSAGLDDGKLKLASGLVQGNNLLALL 154 +LA +A D S D+ P+ SG+++G + Sbjct: 111 KLA------LAMDEAAIS---------DWSVPL----------FSKVSGVLKGEGV---- 141 Query: 155 QNWTAQLPANIDFARLPIPFRAVATDLSLGTEVVLDHGSLPRAMRASMAVPGLFAPFSID 214 QN+ + N+ + IPF AVATDL G ++ G+ A+RAS AVPG+F I Sbjct: 142 QNYVNKALNNVPIEKFKIPFGAVATDLRSGQPILFQRGNAGIAVRASSAVPGVFQSVKIG 201 Query: 215 QRTLVDGGLVSNLPVQTARDMGADVVIAVNIATPLQDAAQLQSPTAVAQQMVGILIQQNV 274 T VDGGLVS +PV+ AR MGAD VIAVNI+ + A S V Q I+ Q Sbjct: 202 DHTYVDGGLVSPVPVRFARQMGADFVIAVNISANPEAQAASSSSVDVLLQTFAIMGQTIN 261 Query: 275 KAQKALLGAQDILIEPQMTGMSFTDFARGKDGINAGWDAA 314 + + L DI+++P + M +DFA I AG AA Sbjct: 262 QYE---LKNADIVLQPGLGAMKGSDFASRNVAIMAGEQAA 298 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,406,485 Number of extensions: 108028 Number of successful extensions: 322 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 320 Number of HSP's successfully gapped: 2 Length of query: 753 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 661 Effective length of database: 1,184,250 Effective search space: 782789250 Effective search space used: 782789250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)