BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2052.p (473 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311514|ref|YP_003775606.1| two component sensor histidine ... 779 0.0 gi|300313895|ref|YP_003777987.1| two-component system sensor his... 117 2e-27 gi|300311468|ref|YP_003775560.1| oxidative stress resistance two... 107 2e-24 gi|300310848|ref|YP_003774940.1| two component sensor histidine ... 104 2e-23 gi|300309980|ref|YP_003774072.1| two component transmembrane sen... 98 1e-21 >gi|300311514|ref|YP_003775606.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 473 Score = 779 bits (2012), Expect = 0.0, Method: Compositional matrix adjust. Identities = 400/474 (84%), Positives = 428/474 (90%), Gaps = 2/474 (0%) Query: 1 VGRLFWKFFF-IAIAQITATFGVGVLFWVENLERSNRNAEIDSSPPASFMIRAAAATLQF 59 +GRLFWKFFF IAIAQITATFGVGVLFWVENLERSNRN+EID SPPASFMIR+AAATLQF Sbjct: 1 MGRLFWKFFFFIAIAQITATFGVGVLFWVENLERSNRNSEIDLSPPASFMIRSAAATLQF 60 Query: 60 GGPEALRSLINEGRPHRMQLYAVNEENQDLMGREVPQHLVEQARHLLTTENERRVVELVK 119 GG EALRSLINEGRPHR+QLYA++E+N+DLMGREV LVEQA+H+L TE ERRVVE VK Sbjct: 61 GGTEALRSLINEGRPHRLQLYALDEDNKDLMGREVSPKLVEQAKHILATERERRVVERVK 120 Query: 120 AANGHSYLMFIQLPTDEFGRPPDDGILQHEARYMKRHGVKPLFFPTMPIAVALVASLVFA 179 A NGHSYLMFI P DEFG G + R +R G K +FFPTMPI VAL+ASLVFA Sbjct: 121 APNGHSYLMFIPAPNDEFGPGGPPGGAREPHRIGER-GPKSMFFPTMPIVVALLASLVFA 179 Query: 180 LLLAWYFSHPIRHLKNAFNQAANGNLDLALEPVMNARNDELADLGRDFDAMTAKLRTLME 239 LLLAWYFSHPI HLKNAFNQAANGNLDL LEPVM+ARNDELADLGRDFD MTAKLRTLME Sbjct: 180 LLLAWYFSHPIGHLKNAFNQAANGNLDLELEPVMSARNDELADLGRDFDVMTAKLRTLME 239 Query: 240 SQRRLLHDVSHELRSPIARLQMAIGLARVQDKDKLELTLERIERESVRMDKLVGELLTLS 299 SQRRLLHDVSHELRSPIARLQ+AIGLARVQDKDKL+LTLERIERESVRMDKLVGELLTLS Sbjct: 240 SQRRLLHDVSHELRSPIARLQVAIGLARVQDKDKLDLTLERIERESVRMDKLVGELLTLS 299 Query: 300 RLEANVPGSMEEPIAIQAMLADMTNDASFEAEALGKRVESHIDCRVQVKGNPELLYRAIE 359 RLEANVPGSMEEPIAI+AML DM NDASFEAEA+GK V+ +DCRVQVKGNPELLYRAIE Sbjct: 300 RLEANVPGSMEEPIAIEAMLGDMINDASFEAEAIGKHVDDQLDCRVQVKGNPELLYRAIE 359 Query: 360 NVLRNAIKHTAPETAVQFVAEIDEFNKLLHLSILDRGPGVPEEELNSIFELFFRSSRTKS 419 NVLRNAIKHTAP T VQ ++DE NKLLHLSILDRGPGVPEEE+NSIFELFFRSSRTKS Sbjct: 360 NVLRNAIKHTAPGTTVQIHTQLDEINKLLHLSILDRGPGVPEEEMNSIFELFFRSSRTKS 419 Query: 420 NSAGHGLGLSIARRIVEAHQGFIHAANRNDGGLRVDITLPFMADEPVRGQTLES 473 NS+GHGLGLSIARRIVEAHQGFI AANR++GGLRVDITLPFMADEP RG T+ES Sbjct: 420 NSSGHGLGLSIARRIVEAHQGFIQAANRSNGGLRVDITLPFMADEPARGDTVES 473 >gi|300313895|ref|YP_003777987.1| two-component system sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 117 bits (292), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 94/300 (31%), Positives = 152/300 (50%), Gaps = 17/300 (5%) Query: 168 IAVALVASLVFALL---LAWYFSHPIRHLKNAFNQAANGNLDLALEPVMNARNDELADLG 224 I +A+VA ++ A L ++ + P+ + A ++ A+G+ + +E DE+ L Sbjct: 175 IIIAVVAVMLAAFLVVRISGVLTRPLTRVARATHRLASGDYAIRVEV---ESGDEVGMLA 231 Query: 225 RDFDAMTAKLRTLMESQRRLLHDVSHELRSPIARLQMAIGLARVQDKDKLE---LTLERI 281 +DF+ + L + +R + DVSHELR+P+A L G + L+ TL + Sbjct: 232 QDFNQLAMTLERNEKLRREFMADVSHELRTPLAILN---GQLEALEDGVLQPDAQTLRSL 288 Query: 282 ERESVRMDKLVGELLTLSRLEANVPGSMEEPIAIQAMLADMTNDASFEAEALGKRVESHI 341 + E ++KLVG+L LS +A I + +L D S G R+ + Sbjct: 289 KSEVEHLNKLVGDLYDLSLADAGALTYRRADIDLAPLLRDSAALYSDRFSQRGMRLSCEL 348 Query: 342 DCRVQVKGNPELLYRAIENVLRNAIKHTAPETAVQFVAEIDEFNKLLHLSILDRGPGVPE 401 + V G+ L + + N+ N++++T +Q A + + + HL D PGVP Sbjct: 349 PASMPVHGDAARLQQLLSNLFENSLRYTDAGGRLQVHAHLQQ--QHWHLVFDDSEPGVPP 406 Query: 402 EELNSIFELFFR--SSRTKSNSAGHGLGLSIARRIVEAHQGFIHAANRNDGGLRVDITLP 459 E + +FE FFR +SR ++ S G GLGL+I RRIV+AHQG I A+ GGLRV + LP Sbjct: 407 EVMARLFERFFRVEASRNRA-SGGAGLGLAICRRIVDAHQGTIGASASPLGGLRVVVVLP 465 >gi|300311468|ref|YP_003775560.1| oxidative stress resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 455 Score = 107 bits (267), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 116/471 (24%), Positives = 211/471 (44%), Gaps = 52/471 (11%) Query: 4 LFWKFFFIAIAQITATFGVGVLFWVEN---LERSNRNAEIDSSPPASFMIRAAAATLQFG 60 LFW+ FF+ ITA+ ++ WV + +ER R ++ + + I AA L Sbjct: 16 LFWRTFFLLTFLITAS----MVAWVASFRMVERGPRAEQLAAQIVSVVTITRAA--LTHS 69 Query: 61 GPEALRSLI-----NEGRPHRMQLYAVNEENQDLMGREVPQHLVEQARHLLTTENERRVV 115 PE R L+ NEG +++Y + + ++ + + P +V + + + + + Sbjct: 70 APEMRRELLFDLASNEG----IRIYPLEKSDRIVPPEDSP--IVPELEYYVRQSLDENTL 123 Query: 116 ELVKAANGHSYLMFIQLPTDEFGRPPDDGILQHEARYMKRHGVKPLFFPTMPIAVALVAS 175 + + + + D++ D G R + ++ L + + +AL S Sbjct: 124 FASSVNDVEGFWISFNIDDDKYWLMLDRG------RLDRTSSLQWLGWGS----IALFLS 173 Query: 176 LVFALLLAWYFSHPIRHLKNAFNQAANGNLDLALEPVMNARNDELADLGRDFDAMTAKLR 235 L+ A+ ++ + P+ L A A G EP+ + E+ + R F+ M A L Sbjct: 174 LIGAVFISTLINQPLARLTAAARAIAKGR---QPEPLPESGPTEIEEANRSFNQMVADLN 230 Query: 236 TLMESQRRLLHDVSHELRSPIARLQMAIGLARVQDKDKLELTLERIERESVRMDKLVGEL 295 + + +L +SH+LR+P+AR+Q+ + +A + D+ + + + + +MD ++G+ Sbjct: 231 RIESDRALILAGISHDLRTPLARMQLEVEMANLSDEAR-----DGMHSDLAQMDSIIGQF 285 Query: 296 LTLSRLEANVPGSMEEPIAIQAMLADMTNDASFEAEALGKRVESHIDCRVQVKGNPELLY 355 L ++ S E I + +L D+ +AS ++ RV + I QV GN L Sbjct: 286 LDYAK---PFDTSSLETIDLADVLQDVAGNASRLSDI---RVHTQITPDTQVAGNGTELA 339 Query: 356 RAIENVLRNAIKHTAPETAVQFVAEIDEFNKL----LHLSILDRGPGVPEEELNSIFELF 411 R N++ NA ++ E AEID +++ + + + D GPGVP E + F Sbjct: 340 RVFSNLVENARRYGKTEGTD--CAEIDIRSRIEGHQVVVEVADHGPGVPVNECERLLRPF 397 Query: 412 FRSSRTKSNSAGHGLGLSIARRIVEAHQG--FIHAANRNDGGLRVDITLPF 460 R + + G GLGL+I RIV H G + GL V ITLP Sbjct: 398 TRLDTARGQANGSGLGLAIVNRIVLKHNGKLLLKGHEETRSGLLVQITLPL 448 >gi|300310848|ref|YP_003774940.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 465 Score = 104 bits (259), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 89/292 (30%), Positives = 142/292 (48%), Gaps = 27/292 (9%) Query: 176 LVFALLLAWYF----SHPIRHLKNAFNQAANGNLDLALEPVMNARNDELADLGRDFDAMT 231 LV +LAW + PIR L A ++ + D+ P+ E+ F+AM Sbjct: 189 LVLIAMLAWLVARMATRPIRQLAQAASRLGS---DIDHAPLAETGPTEIRQAAHAFNAMQ 245 Query: 232 AKLRTLMESQRRLLHDVSHELRSPIARLQMAIGLARVQDKDKLELTLERIERESVRMDKL 291 A++R ++ + +L ++H+L++P+ R M + L +V D L + I+ V M + Sbjct: 246 ARIRRQIQHRTHMLAAITHDLQTPLTR--MRLRLEKVSDPG---LQQKLIDDLGV-MHGM 299 Query: 292 VGELLTLSRLEANVPGSMEEPIAIQAMLAD-MTNDASFEAEALGKRVESHIDCRVQVKGN 350 V E L L+R SM+ IQA+ D + + +A G+ V R V Sbjct: 300 VREGLDLAR-------SMDSSEKIQALDIDSLLDSVCADAADAGQDVALQGSTRAFVMAQ 352 Query: 351 PELLYRAIENVLRNAIKHTAPETAVQFVAEIDEFNKLLHLSILDRGPGVPEEELNSIFEL 410 P L R + N++ NA K+ V E + + + I D+GPG+PE EL ++FE Sbjct: 353 PGALRRCLTNLIDNACKY-GKSARVSIALE----QQKIAIRIRDQGPGIPESELEAVFEP 407 Query: 411 FFRSSRTKS-NSAGHGLGLSIARRIVEAHQGFIHAANRNDGGLRVDITLPFM 461 F+R ++S ++ G GLGL+IA I E H G + N +GGL V + LP M Sbjct: 408 FYRLEASRSRDTGGTGLGLTIALNIAENHHGQLRLRNLAEGGLEVLLELPPM 459 >gi|300309980|ref|YP_003774072.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 390 Score = 98.2 bits (243), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 80/276 (28%), Positives = 140/276 (50%), Gaps = 16/276 (5%) Query: 176 LVFALLLAWY-FSHPIRHLKNAFNQAANGNLDLALEPVMNAR-NDELADLGRDFDAMTAK 233 L AL L W+ S + L Q L P+ + + +E++ L R + M A+ Sbjct: 83 LPVALALVWFALSRGLSPLSE-LQQRIRARRPDDLSPIDSGQVPEEISPLVRSLNDMLAR 141 Query: 234 LRTLMESQRRLLHDVSHELRSPIARLQMAIGLARVQDKDKLEL--TLERIERESVRMDKL 291 L + Q+R + D +H++++P+A ++M LA QD D++++ +LE++ + S +L Sbjct: 142 LSQTIAIQKRFIADAAHQMKTPLAGMRMQSELALRQD-DQVDVRRSLEQLAKSSETATRL 200 Query: 292 VGELLTLSRLE--ANVPGSMEEPIAIQAMLADMTNDASFEAEALGKRVE---SHIDCRVQ 346 V +LL+L+R E + G M P+ + + + D + + +R++ D + Sbjct: 201 VNQLLSLARAENQQSQSGGMI-PVELNELARSVVQD--WVPMSFTQRIDLGFEQPDHPIL 257 Query: 347 VKGNPELLYRAIENVLRNAIKHTAPETA--VQFVAEIDEFNKLLHLSILDRGPGVPEEEL 404 + GNP +L + N+L NA+ +T + V A DE L L + D GPG+P E Sbjct: 258 ITGNPVMLRELLSNLLDNALHYTPHDGNGRVTVRARTDEGGGLAILEVEDNGPGIPVAER 317 Query: 405 NSIFELFFRSSRTKSNSAGHGLGLSIARRIVEAHQG 440 ++FE F+R T S +G GLGL+I R I + H+ Sbjct: 318 ANVFERFYRILGTPSTGSGLGLGLAIVREIAQQHEA 353 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,402,736 Number of extensions: 56478 Number of successful extensions: 341 Number of sequences better than 1.0e-08: 35 Number of HSP's gapped: 287 Number of HSP's successfully gapped: 35 Length of query: 473 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 384 Effective length of database: 1,198,455 Effective search space: 460206720 Effective search space used: 460206720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)