BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2048.p (804 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311510|ref|YP_003775602.1| phosphoenolpyruvate synthase/py... 1291 0.0 gi|300309436|ref|YP_003773528.1| phosphoenolpyruvate-protein pho... 86 1e-17 >gi|300311510|ref|YP_003775602.1| phosphoenolpyruvate synthase/pyruvate phosphate dikinase [Herbaspirillum seropedicae SmR1] Length = 791 Score = 1291 bits (3340), Expect = 0.0, Method: Compositional matrix adjust. Identities = 631/640 (98%), Positives = 636/640 (99%) Query: 1 MSNAVNTQGAVYVASFENLRMTDVESVGGKNASLGEMISQLAGAGVRVPGGFATTAQAFR 60 MSNA NTQGAVYVASFE LRMTDVESVGGKNASLGEMISQLAGAGVRVPGGFATTAQAFR Sbjct: 1 MSNAANTQGAVYVASFETLRMTDVESVGGKNASLGEMISQLAGAGVRVPGGFATTAQAFR 60 Query: 61 DFLEHSADGGPTLAARIAKRLEGLDIDDVRALAQAGAEIRQWIVETPFQPRLDQEIRAFY 120 DFLEHSADGGPTLAARIAKRLEGLDIDDVR+LAQAGAEIRQWI++TPFQPRLDQEIRAFY Sbjct: 61 DFLEHSADGGPTLAARIAKRLEGLDIDDVRSLAQAGAEIRQWIIDTPFQPRLDQEIRAFY 120 Query: 121 DKLVADSTAEMSFAVRSSATAEDLPDASFAGQQETFLNVVGIDNVLEAMKHVFASLYNDR 180 DKLVADSTAEMSFAVRSSATAEDLPDASFAGQQETFLNVVGIDNVLEAMKHVFASLYNDR Sbjct: 121 DKLVADSTAEMSFAVRSSATAEDLPDASFAGQQETFLNVVGIDNVLEAMKHVFASLYNDR 180 Query: 181 AISYRVHKGFTHAEVALSAGVQRMVRSDVGAAGVMFTIDTESGFKDVVFITSSYGLGETV 240 AISYRVHKGFTHAEVALSAGVQRMVRSDVGAAGVMFTIDTESGFKDVVFITSSYGLGETV Sbjct: 181 AISYRVHKGFTHAEVALSAGVQRMVRSDVGAAGVMFTIDTESGFKDVVFITSSYGLGETV 240 Query: 241 VQGAVNPDEYYVHKPMLELGKQPVIRRNLGSKLIKMEFTGEAKAGRSVKTVDVPVELRNR 300 VQGAVNPDEYYVHKPMLELGKQPVIRRNLGSKLIKMEFTGEAKAGRSVKTVDVPVELRNR Sbjct: 241 VQGAVNPDEYYVHKPMLELGKQPVIRRNLGSKLIKMEFTGEAKAGRSVKTVDVPVELRNR 300 Query: 301 YSLTDAEVVELAKYATIIEKHYQRPMDIEWGKDGRDGKLYILQARPETVKSQQKATDAQQ 360 YSLTDAEVVELAKYATIIEKHYQRPMDIEWGKDGRDGKLYILQARPETVKSQQKATDAQQ Sbjct: 301 YSLTDAEVVELAKYATIIEKHYQRPMDIEWGKDGRDGKLYILQARPETVKSQQKATDAQQ 360 Query: 361 RFKLKSSGTVLATGRAIGQKIGAGPVRVISDPAEMERVQPGDVLVADMTDPNWEPVMKRA 420 RFKLKSSGTVLATGRAIGQKIGAGPVRVI+DPAEMERVQPGDVLVADMTDPNWEPVMKRA Sbjct: 361 RFKLKSSGTVLATGRAIGQKIGAGPVRVITDPAEMERVQPGDVLVADMTDPNWEPVMKRA 420 Query: 421 SAIVTNRGGRTCHAAIIARELGVPAVVGCGDATEVLKDGNLVTVSCAEGDEGKIYDGLLE 480 SAIVTNRGGRTCHAAIIARELGVPAVVGCGDAT+VLKDG LVTVSCAEGDEGKIYDGLLE Sbjct: 421 SAIVTNRGGRTCHAAIIARELGVPAVVGCGDATDVLKDGTLVTVSCAEGDEGKIYDGLLE 480 Query: 481 TEVTEVARGELPPIPLKVMMNVGNPQLAFDFQSIPNGGVGLARLEFIINNNIGVHPKAIL 540 TEVTEVARGELPPIPLKVMMNVGNPQLAFDFQSIPNGGVGLARLEFIINNNIGVHPKAIL Sbjct: 481 TEVTEVARGELPPIPLKVMMNVGNPQLAFDFQSIPNGGVGLARLEFIINNNIGVHPKAIL 540 Query: 541 EYPNIDADLKKAVESVARGHASPKAFYVDKLAEGIATIAAAFWPKPVIVRLSDFKSNEYK 600 EYPNIDADLKKAVESVARGHASPKAFYVDKLAEGIATIAAAFWPKPVIVRLSDFKSNEYK Sbjct: 541 EYPNIDADLKKAVESVARGHASPKAFYVDKLAEGIATIAAAFWPKPVIVRLSDFKSNEYK 600 Query: 601 KLIGGSRYEPDEENPMLGFRGAARYLAHDFAEAFQMECQA 640 KLIGGSRYEPDEENPMLGFRGAARYLA DFAEAFQMECQA Sbjct: 601 KLIGGSRYEPDEENPMLGFRGAARYLADDFAEAFQMECQA 640 >gi|300309436|ref|YP_003773528.1| phosphoenolpyruvate-protein phosphotransferase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 595 Score = 85.5 bits (210), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 97/410 (23%), Positives = 181/410 (44%), Gaps = 81/410 (19%) Query: 424 VTNRGGRTCHAAIIARELGVPAVVGCGDATEVLKDGNLVTVSCAEGDEGKIY-------- 475 VT+ GG H AI+AR L +PA VG +A+ ++K +++ + +GD G + Sbjct: 186 VTDLGGPNSHTAIVARSLAIPAAVGMHNASVLVKHDDVLII---DGDAGVVIVDPSPLVL 242 Query: 476 ------DGLLETEVTEVARGELPPI------PLKVMMNV---GNPQLAFDFQSIPNGGVG 520 L E +++R + P P+ ++ N+ G+ AF+ ++ G+G Sbjct: 243 AQYRARQERLLRERKKLSRLKKTPAITQDGEPITLLANIELPGDAVTAFEAGAM---GIG 299 Query: 521 LARLEFIINNNIGVHPKAILEYPNIDADLKKAVESVARGHASPKAFYVDKLAEGIATIAA 580 L R EF+ G + ++P+ D + ++V Sbjct: 300 LFRSEFLFMGRAG----HLEKFPSEDEQFEHYKQAVI----------------------- 332 Query: 581 AFWPKPVIVRLSDFKSNEYKKLIGGSRYEPDEE---NPMLGFRGAARYLAHDFAEAFQME 637 A + V +R D +++ + DEE NP LG R A RY + + F + Sbjct: 333 AMKGRSVTIRTLDVGADK--------PLDEDEETALNPALGLR-AIRYCLSE-PQMFLTQ 382 Query: 638 CQAMKRVREDMGLTNVEVMVPFVRTLGQAEKVIGLL--GKYGLK----RGENGLRVIMMC 691 +A+ R ++++VP + + ++ + ++ K L+ + + + V M Sbjct: 383 LRAILRAS---AFGPIKLLVPMMAHAFEIDQTLAMIDQAKQQLRERKQKFDEEIPVGAMI 439 Query: 692 EVPSNAILADQFLEHFDGFSIGSNDLTQLTLGLDRDSGMELLAADFDERDPAVQALLTKV 751 E+P+ A+ F++ D SIG+NDL Q TL +DR +A +D PAV LL+ V Sbjct: 440 EIPAAALALPLFVKRLDFLSIGTNDLIQYTLAIDRVD--HEVAHLYDPLHPAVLFLLSTV 497 Query: 752 ITTCREKGKYVGICGQGPSDHPDFAQWLMEQGIESISLNPDTVIDTWQQL 801 I+ R+ G V +CG+ D + + L+ G+ S++P ++ ++ Sbjct: 498 ISQGRKAGIPVSVCGEMAGDL-KWTRLLLGMGLLEFSMHPSQILSVKNEI 546 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,534,902 Number of extensions: 111996 Number of successful extensions: 331 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 331 Number of HSP's successfully gapped: 2 Length of query: 804 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 711 Effective length of database: 1,179,515 Effective search space: 838635165 Effective search space used: 838635165 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)