BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2016.p (403 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotr... 786 0.0 gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic ami... 129 6e-31 gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent a... 91 2e-19 gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Her... 82 8e-17 gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic ami... 72 1e-13 >gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 420 Score = 786 bits (2029), Expect = 0.0, Method: Compositional matrix adjust. Identities = 381/403 (94%), Positives = 393/403 (97%) Query: 1 VNPLLDKLQPYPFEKLKKLFAGITPDPAYRPISLGIGEPKHPTPAFIQQALTDNLAGLAS 60 VNPLLD+LQPYPFEKLKKLFAGITPDPAYRPISLGIGEPKHPTPAFIQQAL DNLAGLAS Sbjct: 18 VNPLLDQLQPYPFEKLKKLFAGITPDPAYRPISLGIGEPKHPTPAFIQQALIDNLAGLAS 77 Query: 61 YPATLGSEALRGAIADWLQQRYDILRPDPATEILPVIGSREALFSLTQTVVDPTRPGALV 120 YPATLGS+ALRGAIADWLQ+RYDI RPDPATEILPV GSREALFSLTQTVVDPTRPGALV Sbjct: 78 YPATLGSDALRGAIADWLQKRYDIPRPDPATEILPVNGSREALFSLTQTVVDPTRPGALV 137 Query: 121 MCPNPFYQIYEGSAYLAGAEPYFVNSDPARNFAPDFSQVPQEVWERVQLLFVCSPGNPTG 180 MCPNPFYQIYEGSAYLAGA+PYFVNSDP RNFAPDFSQVPQ+VWERVQLLFVCSPGNPTG Sbjct: 138 MCPNPFYQIYEGSAYLAGAQPYFVNSDPKRNFAPDFSQVPQDVWERVQLLFVCSPGNPTG 197 Query: 181 AVLKLEDWKELFALSDRYGFVIASDECYSEIYFKQDPPLGGMEAARKLGRDYRNLVCFSS 240 AVL LEDWKELFALSDRYGFVIASDECYSEIYF +DPPLGGM+AAR LGRDYR L+ FSS Sbjct: 198 AVLTLEDWKELFALSDRYGFVIASDECYSEIYFNEDPPLGGMQAARLLGRDYRRLISFSS 257 Query: 241 LSKRSNVPGMRSGFVAGDAAILKKFLLYRTYHGAAMSPVIQAASVLAWQDETHVQDNIAK 300 LSKRSNVPGMRSGFVAGDAAILKKFLLYRTYHGAAMSPVIQAASVLAWQDETHVQ NIAK Sbjct: 258 LSKRSNVPGMRSGFVAGDAAILKKFLLYRTYHGAAMSPVIQAASVLAWQDETHVQGNIAK 317 Query: 301 YVTKFSQVTPLLQEVMDVALPDAAFYLWAKVDKLVDISDTEFARRLYAEYNVTVLPGSYL 360 YVTKFSQVTPLLQEV++VALPDAAFYLWAKVDKLV+ISDTEFA+RLYAEYNVTVLPGSYL Sbjct: 318 YVTKFSQVTPLLQEVLEVALPDAAFYLWAKVDKLVNISDTEFAQRLYAEYNVTVLPGSYL 377 Query: 361 AREAHGVNPGAGRIRMALVAEVEECLEAAQRIVAFTRKLAGQA 403 AREAHGVNPGAGRIRMALVAEVEECLEAAQRIVAFTRKLAGQA Sbjct: 378 AREAHGVNPGAGRIRMALVAEVEECLEAAQRIVAFTRKLAGQA 420 >gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 431 Score = 129 bits (323), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 108/406 (26%), Positives = 185/406 (45%), Gaps = 24/406 (5%) Query: 3 PLLDKLQPYPFEKLKKLFAGITPDPAYRPISLGIGEPKHPTPAFIQQALTD--NLAGLAS 60 P +++L PY F +L I + +G P TP I + L + Sbjct: 42 PRIERLPPYVFNITAELKMAARRR-GEDIIDMSMGNPDGATPPHIVEKLVEVAQRPDTHG 100 Query: 61 YPATLGSEALRGAIADWLQQRYDILRPDPATEILPVIGSREALFSLTQTVVDPTRPGALV 120 Y ++ G LR AIA W + RY++ DP +E + IGS+E L L +D G V Sbjct: 101 YSSSKGIPRLRRAIAHWYRSRYEV-DFDPDSEAIVTIGSKEGLAHLMLATLDK---GDTV 156 Query: 121 MCPNPFYQIYEGSAYLAGAEPYFVNSDPARNFAPDFSQVPQEVWERVQLLFVCSPGNPTG 180 + PNP Y I+ A +AGA V P +F + + +E + + +++ + P NPT Sbjct: 157 LVPNPSYPIHIYGAVIAGANIRSVRMSPGVDFFDELERAVRESYPKPKMMILGFPSNPTA 216 Query: 181 AVLKLEDWKELFALSDRYGFVIASDECYSEIYFK--QDPPLGGMEAARKLGRDYRNLVCF 238 ++LE ++ + L+ + ++ D Y++I F + P + + AR++ V F Sbjct: 217 QCVELEFFERVVKLAREHQILVVHDLAYADITFDGWKAPSIMQVPGAREVA------VEF 270 Query: 239 SSLSKRSNVPGMRSGFVAGDAAILKKFLLYRTYHGAAMSPVIQAASVLAWQ-DETHVQDN 297 +LSK N+ G R GF+ G+A ++ ++YH +Q A++ A + D++ V++ Sbjct: 271 FTLSKSYNMAGWRIGFMVGNARLVAALARIKSYHDYGSFTPVQVAAIAALEGDQSCVEEI 330 Query: 298 IAKYVTKFSQVTPLLQEV-MDVALPDAAFYLWAKV-DKLVDISDTEFARRLYAEYNVTVL 355 A Y + + + L E V +P A+ Y+WA++ + EFAR L + V V Sbjct: 331 RANYERRRNVLVKGLHEAGWMVDVPKASMYIWARIPEPYRQFGSLEFARILLEQAKVCVS 390 Query: 356 PGSYLAREAHGVNPGAGRIRMALVAEVEECLEAAQRIVAFTRKLAG 401 PG G +R AL+ +A + I A + G Sbjct: 391 PGIGFGEY------GDEYVRFALIENESRIRQAIRGIKAMFKNAKG 430 >gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 90.9 bits (224), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 86/330 (26%), Positives = 138/330 (41%), Gaps = 11/330 (3%) Query: 32 ISLGIGEPK-HPTPAFIQQALTDNLAGLASYPATLGSEALRGAIADWLQQRYDILRPDPA 90 ++LG G P H PA + GL YP G LR AIA+ +++ Y R DP Sbjct: 31 VNLGQGFPDFHCDPALVAAVTQAMQEGLNQYPPMAGVPVLREAIAEKVEKLYG-HRYDPV 89 Query: 91 TEILPVIGSREALFSLTQTVVDPTRPGALVMCPNPFYQIYEGSAYLAGAEPYFVNSD-PA 149 +EI G+ + + + +V+ PG V+ P Y Y + LAG P FV + A Sbjct: 90 SEITVTAGATQGILT---SVLCAVHPGDEVIVIEPVYDCYVPAIELAGGVPVFVQMEVGA 146 Query: 150 RNFAPDFSQVPQEVWERVQLLFVCSPGNPTGAVLKLEDWKELFALSDRYGFVIASDECYS 209 + ++ + +V V + +++ V +P NPTG+V++ D L + +I SDE Y Sbjct: 147 QGYSIPWDKVKAAVTPKTRMIMVNTPHNPTGSVMRAADVAALADIVRGTDILILSDEVYE 206 Query: 210 EIYFKQDPPLGGMEAARKLGRDYRNLVCFSSLSKRSNVPGMRSGFVAGDAAILKKFLLYR 269 + + P R + N SS K +V G + G+VA AA+ +F Sbjct: 207 HMVYDGQPHESLARHPELAERSFIN----SSFGKTYHVTGWKVGYVAAPAALTAEFRKVH 262 Query: 270 TYHGAAMSPVIQAASVLAWQDETHVQDNIAKYVTKFSQVTPLLQEVMDVALPDAAFYL-W 328 ++ ++ +Q +D D A Y K L LP Y Sbjct: 263 QFNVFTVNTPVQYGLAAYMKDPAPYLDLPAFYQHKRDLFRTGLANTRFELLPSQGTYFQC 322 Query: 329 AKVDKLVDISDTEFARRLYAEYNVTVLPGS 358 K + + + EF + L +E V +P S Sbjct: 323 VKYGAISALPEAEFCKWLTSEIGVAAIPVS 352 >gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 409 Score = 82.0 bits (201), Expect = 8e-17, Method: Compositional matrix adjust. Identities = 90/394 (22%), Positives = 165/394 (41%), Gaps = 49/394 (12%) Query: 26 DPAYRPISLGIGEPK---HPTPAFIQQALTDNLAGLASYPATLGSEALRGAIADWLQQRY 82 + ++ I L IG P I Q + N+ + Y + G A R ++ + QQ+ Sbjct: 30 EEGHKIIKLNIGNLAAFGFDAPDEIVQDMIRNMGNASGYTDSKGLFAPRKSVMHYTQQKK 89 Query: 83 -------DILRPDPATEILPVIGSREALFSLTQTVVDPTRPGALVMCPNPFYQIYEGSAY 135 DI + A+E+ ++ S AL + G V+ P+P Y ++ + Sbjct: 90 IEGVTIDDIYLGNGASEL--IVMSVNALLN----------TGDEVLVPSPDYPLWTAAVS 137 Query: 136 LAGAEPYFVNSDPARNFAPDFSQVPQEVWERVQLLFVCSPGNPTGAVLKLEDWKELFALS 195 L+G P D + + PD + + +++ + + V +P NPTGA+ +E KE+ L+ Sbjct: 138 LSGGTPVHYVCDEQQGWQPDIADIKKKITPNTKAIVVINPNNPTGALYSVEVLKEIIELA 197 Query: 196 DRYGFVIASDECYSEIYFKQDPPLGGMEAARKLGRDYRNLVCFSSLSKRSNVPGMRSGF- 254 ++ +I +DE Y ++ + G + D + F+ LSK G R+G+ Sbjct: 198 RQHQLIILADEIYDKVLYD-----GNTHTSLASLADDVLFITFNGLSKNYRSCGYRAGWM 252 Query: 255 -VAGDAAILKKFLLYRTYHGAAMSPVIQAASVLAWQDETHVQDNIAKYVTKFSQVTP--- 310 V+G+ + ++ A+M A A Q +I V ++T Sbjct: 253 VVSGEKKHARDYIEGLNML-ASMRLCANAPGQFAIQTALGGYQSINDLVGPQGRLTKQRD 311 Query: 311 ----LLQEVMDVAL--PDAAFYLWAKVDKLV--DISDTEFARRLYAEYNVTVLPGSYLAR 362 LL ++ V+ P +A Y++ ++D + D EFA L AE V ++ G+ Sbjct: 312 LAHQLLTDIPGVSCVKPKSALYMFPRLDPEIYPIKDDQEFAYELLAEEKVLIVQGT---- 367 Query: 363 EAHGVN-PGAGRIRMALVAEVEECLEAAQRIVAF 395 G N P R+ + ++ E+ RI F Sbjct: 368 ---GFNCPTPDHFRVVFLPNTDDLTESMGRIAHF 398 >gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 393 Score = 71.6 bits (174), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 98/411 (23%), Positives = 164/411 (39%), Gaps = 49/411 (11%) Query: 5 LDKLQPYPFEKLKKLFAGITPDPAYRPISLGIGEPKHPTPAFIQQALTDNLA-GLASYPA 63 L + P+ +L K A + I +GIGEP P + +A T + G Y + Sbjct: 10 LQHIAPFHVMELSKK-AAVLEQQGRHLIHMGIGEPDFTAPPAVVEAATRAMTEGKMQYTS 68 Query: 64 TLGSEALRGAIADWLQQRYDILRPDPATEILP---VIGSREALFSLTQTVVDPTRPGALV 120 G LR AI+D + Y + EI P V+ + + L R ++ Sbjct: 69 ATGLPQLRAAISDHYRSVYGL-------EIAPERIVVTAGASAALLLACAALVERDSEVL 121 Query: 121 M------CPNPFYQIYEGSAYLAGAEPYFVNSDPARNFAPDFSQVPQEVWERVQLLFVCS 174 M C F +EG A L + S P F V + + + + S Sbjct: 122 MPDPSYPCNRHFVAAFEGRAKL-------IASGPEHRFQLSAQMVREHWGSATRGVLLAS 174 Query: 175 PGNPTGAVLKLEDWKELFA-LSDRYGFVIASDECYSEIYFKQDPPLGGMEAARKLGRDYR 233 P NPTG + ++ + + + R GF I DE Y + ++ P +A LG D Sbjct: 175 PSNPTGTSILPDELRAIVGEVRQRGGFTIV-DEIYQGLSYEGAP-----FSALSLGDD-- 226 Query: 234 NLVCFSSLSKRSNVPGMRSGFVAGDAAILKKFLLYRTYHGAAMSPVIQAASVLAWQDETH 293 +V +S SK N+ G R G++ A++ + S + Q A+V + +T Sbjct: 227 -VVVINSFSKYFNMTGWRLGWLVLPPALVPQVEKLAQNLLICASSIAQHAAVACFTPQTL 285 Query: 294 V--QDNIAKYVTKFSQVTPLLQEVMDV--ALPDAAFYLWAKVDKLVDISDTEFARRLYAE 349 ++ A++ + + P L+ + +PD AFY++A L D +D + + + E Sbjct: 286 ALYEERKAEFKRRRDYIVPALESLGFTVPVMPDGAFYVYADCSALADDAD-QLSLDMLNE 344 Query: 350 YNVTVLPGSYLAREAHGVNPGAGR--IRMALVAEVEECLEAAQRIVAFTRK 398 V ++PG P R IR++ +E EA R+ F K Sbjct: 345 AGVVLVPGL-------DFGPFTARRYIRLSYATSMENLQEAVARLRVFFEK 388 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,344,862 Number of extensions: 57848 Number of successful extensions: 147 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 141 Number of HSP's successfully gapped: 5 Length of query: 403 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 316 Effective length of database: 1,207,925 Effective search space: 381704300 Effective search space used: 381704300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)