BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1982.p (754 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311444|ref|YP_003775536.1| bifunctional (P)ppGpp synthetas... 1520 0.0 gi|300311292|ref|YP_003775384.1| (p)ppGpp synthetase I [Herbaspi... 357 2e-99 >gi|300311444|ref|YP_003775536.1| bifunctional (P)ppGpp synthetase II/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 753 Score = 1520 bits (3936), Expect = 0.0, Method: Compositional matrix adjust. Identities = 735/754 (97%), Positives = 743/754 (98%), Gaps = 1/754 (0%) Query: 1 MSLTTTDSTLSAAASSKRAAGSGRTATPQEVAPAAAHSGVATFAHLIPKLEEYLTPSELK 60 MSLTTTDSTLSA S KRAAGSGRTA P EVAPAA HSGVATFAHLIPKLEEYLTPSELK Sbjct: 1 MSLTTTDSTLSATPSPKRAAGSGRTAAPTEVAPAA-HSGVATFAHLIPKLEEYLTPSELK 59 Query: 61 KVKEAYRFADEMHLGQMRKSGEPYISHPLAVAEICAEWKLDAQAMMAAFLHDVMEDQGVK 120 KVKEAYRFADEMHLGQMRKSGEPYISHPLAVAEICAEWKLDAQAMMAAFLHDVMEDQGVK Sbjct: 60 KVKEAYRFADEMHLGQMRKSGEPYISHPLAVAEICAEWKLDAQAMMAAFLHDVMEDQGVK 119 Query: 121 KEELIERFGASVASLVDGLSKLDKIEFQSQVEAQAENFRKMLLAMARDVRVILVKLADRL 180 KEELIERFGASVASLVDGLSKLDKIEFQSQVEAQAENFRKMLLAMARDVRVILVKLADRL Sbjct: 120 KEELIERFGASVASLVDGLSKLDKIEFQSQVEAQAENFRKMLLAMARDVRVILVKLADRL 179 Query: 181 HNMRTLGSMPPEKKRRISRETMEVYVPIAHRLGLNNIYRELQELSFSHLYPLRHRTLSKA 240 HNMRTLGSMPPEKKRRISRETMEVYVPIAHRLGLNNIYRELQELSF+HL+PLRHRTLSKA Sbjct: 180 HNMRTLGSMPPEKKRRISRETMEVYVPIAHRLGLNNIYRELQELSFAHLHPLRHRTLSKA 239 Query: 241 VKAARGNRREVVTKIMESVTSTLIAAGIPAQVDGREKTLYGIYRKMRNKHLSFSQVLDVY 300 VKAARGNRREVVTKIMESVTSTLIAAGIPAQVDGREKTLYGIYRKMRNKHLSFSQVLDVY Sbjct: 240 VKAARGNRREVVTKIMESVTSTLIAAGIPAQVDGREKTLYGIYRKMRNKHLSFSQVLDVY 299 Query: 301 GFRVVVDSFANCYVALGTLHSLFKPMPGKFKDYIAIPKLNGYQSLHTTLIGPYGTPVEFQ 360 GFRVVVDSFANCYVALGTLHSLFKPMPGKFKDYIAIPKLNGYQSLHTTLIGPYGTPVEFQ Sbjct: 300 GFRVVVDSFANCYVALGTLHSLFKPMPGKFKDYIAIPKLNGYQSLHTTLIGPYGTPVEFQ 359 Query: 361 IRTTEMHRVAESGVAAHWLYKDDEGSLTDLQQRTHAWLQSLLDIQKQTGDSAEFLEHVKV 420 IRT+EMHRVAESGVAAHWLYKDDEGSLTDLQQRTHAWLQSLLDIQKQTGDSAEFLEHVKV Sbjct: 360 IRTSEMHRVAESGVAAHWLYKDDEGSLTDLQQRTHAWLQSLLDIQKQTGDSAEFLEHVKV 419 Query: 421 DLFPDSVYVFTPKSKIIALPRGATALDFAYSIHTDIGDQTTSVIINHEPAPLRSELHNGD 480 DLFPDSVYVFTPKSKIIALPRGATALDFAYSIHTDIGDQTTS IINHEPAPLR+ELHNGD Sbjct: 420 DLFPDSVYVFTPKSKIIALPRGATALDFAYSIHTDIGDQTTSAIINHEPAPLRAELHNGD 479 Query: 481 IVEIITSPTSRPSPNWLGYVRTGKARSAIRHRLRTANRVESQTVGRRLLGNALHTLGIEP 540 IVEIITSPTSRPSPNWLGYVRTGKARSAIRHRLRTAN+VESQ VGRRLLGNALHTLGIEP Sbjct: 480 IVEIITSPTSRPSPNWLGYVRTGKARSAIRHRLRTANQVESQAVGRRLLGNALHTLGIEP 539 Query: 541 ELPSHIVERLLNESSAKTMDDLYAEIGIGTRMAPLVARHIMTMMDTGVSVPQLDPEGHML 600 ELPSHIVERLLNESSAKTMDDLYAEIGIGTRMAPLVARHIMTMMDTGVSVPQLDPEGHML Sbjct: 540 ELPSHIVERLLNESSAKTMDDLYAEIGIGTRMAPLVARHIMTMMDTGVSVPQLDPEGHML 599 Query: 601 PNKPDPVVITGSEGASAQLSSCCMPIPGDRLTGYLKPDQTLIVHTQECETAKRLHEKEPD 660 PNKPDPVVITGSEGASAQLSSCCMPIPGDRLTGYLK DQTLIVHTQEC+TAKRLHEKEPD Sbjct: 600 PNKPDPVVITGSEGASAQLSSCCMPIPGDRLTGYLKADQTLIVHTQECDTAKRLHEKEPD 659 Query: 661 RWIELVWGHDLNRRFDCRITILIHNERGTLARIAAEIGESDANIVSVAMDDEGGNASMKY 720 RWIELVWG DLNRRFDCRITILIHNERGTLARIAAEIGESDANIVSVAMDDEGGNASMKY Sbjct: 660 RWIELVWGQDLNRRFDCRITILIHNERGTLARIAAEIGESDANIVSVAMDDEGGNASMKY 719 Query: 721 LRFTIQIEDRVHLARLMRGIRKIDSVARILRERG 754 LRFTIQ+EDRVHLAR MRGIR+IDSVARILRERG Sbjct: 720 LRFTIQVEDRVHLARTMRGIRRIDSVARILRERG 753 >gi|300311292|ref|YP_003775384.1| (p)ppGpp synthetase I [Herbaspirillum seropedicae SmR1] Length = 749 Score = 357 bits (915), Expect = 2e-99, Method: Compositional matrix adjust. Identities = 231/742 (31%), Positives = 364/742 (49%), Gaps = 54/742 (7%) Query: 54 LTPSELKKVKEAYRFADEMHLGQMRKSGEPYISHPLAVAEICAEWKLDAQAMMAAFLHDV 113 L P + +V +A + ++ + SG+ + ++VA + +DA +AA + Sbjct: 19 LGPEDAARVAQALAELEPLYADRQILSGQNALQFSISVATVLTALNVDAATRIAALAFEA 78 Query: 114 MEDQGVKKEELIERFGASVASLVDGLSKLDKI---EFQSQVEA------------QAENF 158 +E+L ERFG +A LV + +L + FQ Q E Q E Sbjct: 79 QMLHPELEEKLEERFGKEIADLVGNVRQLMRFHSFSFQPQQEVLRGKNAAQQAAAQIETL 138 Query: 159 RKMLLAMARDVRVILVKLADRLHNMRTLGSMPPEKKR--RISRETMEVYVPIAHRLGLNN 216 RKMLLAMA D+R +LV+LA R+ +R E + +R+T E+Y P+A+RLG+ Sbjct: 139 RKMLLAMATDMRAVLVRLASRVATLRYFAETKQEDEASANYARQTFELYAPLANRLGIWQ 198 Query: 217 IYRELQELSFSHLYPLRHRTLSKAVKAARGNRREVVTKIMESVTSTLIAAGIPAQVDGRE 276 + EL++LSF L P+ ++ ++K ++ R R V + + + + A I A+V GR Sbjct: 199 LKWELEDLSFRFLEPVTYKRIAKMLEEKRLEREAFVANAIARLRAEMAAQDIRAEVSGRP 258 Query: 277 KTLYGIYRKMRNKHLSFSQVLDVYGFRVVVDSFANCYVALGTLHSLFKPMPGKFKDYIAI 336 K +Y I+ KMR KH+ FS++ DV FRV+VD CY LG +H ++ P+P +F DYI+ Sbjct: 259 KHIYSIWNKMRGKHIDFSELYDVRAFRVIVDDVKTCYAVLGIVHDIWTPIPEEFDDYISR 318 Query: 337 PKLNGYQSLHTTLIGPYGTPVEFQIRTTEMHRVAESGVAAHWLYKDDEGSLTDLQQRTH- 395 PK NGYQSLHT + G +E QIRT EMH AE GVAAHW YK+ GS Q+ Sbjct: 319 PKQNGYQSLHTVVTAEDGRALEVQIRTHEMHHFAEYGVAAHWRYKESGGSNFSAQKYDEK 378 Query: 396 -AWLQSLLDIQKQTGDSA--------EFLEHVKVDLFPDSVYVFTPKSKIIALPRGATAL 446 AWL+ LL + + D+ +++E +K + +YV TP++++I LP GAT + Sbjct: 379 IAWLRQLLAWKNEVTDAVVDQEEVRRDWVEKLKSATLDERIYVLTPQARVIELPSGATPI 438 Query: 447 DFAYSIHTDIGDQTTSVIINHEPAPLRSELHNGDIVEIIT---SPTSRPSPNWLG--YVR 501 DFAY +H+D+G + ++ PL + L NG VEIIT P PS +WL Y Sbjct: 439 DFAYHLHSDVGHRCRGARVDGVMVPLNTALKNGQTVEIITVKSGPGVGPSRDWLSPDYAV 498 Query: 502 TGKARSAIRHRLRTANRVESQTVGRRLLGNALHTLGIEPELPSHIVERLLNESSAKTMDD 561 + + RS IR + E+ +VGR LL L G + +E L + +DD Sbjct: 499 SSRTRSKIRAWFNAIEQQETLSVGRGLLEKTLQREG----KTAVNLEELAQKLGFAKVDD 554 Query: 562 LYAEIGIGTRMAPLVARHIMTMMDTGV-SVPQLDPE---------GHMLPNKPDPVVITG 611 L + RH+ + G P++D E ++ V++ G Sbjct: 555 LCLSLA----KEEFSLRHVEQALHEGQEKKPEIDDETLITRKSRASSIVQGAKSGVLVVG 610 Query: 612 SEGASAQLSSCCMPIPGDRLTGYLKPDQTLIVHTQECETAKRLHEKEPDRWIELVWGHDL 671 ++G QL+ CC P P D + G++ + + +H C+ + P+R I+ WG Sbjct: 611 TDGLLTQLARCCKPAPPDAIAGFVTRGKGVSIHRVSCKNFAEMSSHAPERVIQTTWGAPA 670 Query: 672 NRR-FDCRITILIHNERGTLARIAAEIGESDANIVSVAMDDEGGNASMKYLRFTIQIEDR 730 + I +L + +G L I+ N++ V+ G+A M FT +I Sbjct: 671 RETVYPVDIFVLASDRQGLLRDISDIFLRDKINVIGVSTQSVKGHARMA---FTAEIGST 727 Query: 731 VHLARLMRGIRKIDSVARILRE 752 L + + IR++ V R+ Sbjct: 728 EQLQKALTVIREVKGVIEAKRQ 749 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,245,993 Number of extensions: 94011 Number of successful extensions: 305 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 300 Number of HSP's successfully gapped: 2 Length of query: 754 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 662 Effective length of database: 1,184,250 Effective search space: 783973500 Effective search space used: 783973500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)