BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1973.p (555 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311435|ref|YP_003775527.1| chloride channel protein [Herba... 1001 0.0 gi|300310913|ref|YP_003775005.1| chloride channel EriC protein [... 108 1e-24 gi|300310046|ref|YP_003774138.1| chloride-channel protein [Herba... 75 1e-14 gi|300311782|ref|YP_003775874.1| chloride channel protein [Herba... 70 4e-13 >gi|300311435|ref|YP_003775527.1| chloride channel protein [Herbaspirillum seropedicae SmR1] Length = 555 Score = 1001 bits (2588), Expect = 0.0, Method: Compositional matrix adjust. Identities = 523/555 (94%), Positives = 544/555 (98%) Query: 1 MLLWGALAGFVGALATIAFRECIALLQIWLTGHDGSFVDIAKGLPWHVRVLLPTCGGVVA 60 MLLWGALAGFVGALATIAFRECIALLQIW+TGHDGSFVDIAKGLPW+ RVLLPTCGGVVA Sbjct: 1 MLLWGALAGFVGALATIAFRECIALLQIWMTGHDGSFVDIAKGLPWYTRVLLPTCGGVVA 60 Query: 61 GLFLVWAKKTPAGNGGDYMEAVAIGDGAIPVRNTLLRSISSLASIASGGSIGREGPMVQL 120 GLFLVWAK+TPAGNGGDYMEAVAIGDGAIPVRNTLLRSISSLASIASGGSIGREGPMVQL Sbjct: 61 GLFLVWAKRTPAGNGGDYMEAVAIGDGAIPVRNTLLRSISSLASIASGGSIGREGPMVQL 120 Query: 121 SALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGAFFITEIVLGSLVMESFGP 180 SALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGAFFITEIVLGSLVMESFGP Sbjct: 121 SALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGAFFITEIVLGSLVMESFGP 180 Query: 181 VMVASVVANITMRELPGYRATYEMPYFPEIGGWEVLLFLVLGVLAGILAPQFLRVLELGK 240 V+VASVVANITMRELPGYRA YEMPYFPEIGGWEV+LFLVLGVLAG+LAPQFLRVLELGK Sbjct: 181 VVVASVVANITMRELPGYRAAYEMPYFPEIGGWEVMLFLVLGVLAGVLAPQFLRVLELGK 240 Query: 241 HGFAKFKLPLPVRLGVGGLLVGLISIQVPEVWGNGYSLVNSLLHTTWLWQMVLMVLVIKV 300 HGF K +LPLPVRLGVGGLLVGLIS+QVPEVWGNGYSLVNSLLHT+W+WQ VLMVLV+KV Sbjct: 241 HGFGKLRLPLPVRLGVGGLLVGLISVQVPEVWGNGYSLVNSLLHTSWVWQAVLMVLVVKV 300 Query: 301 LATAITVGSGAVGGIFTPTLFVGAAVGYLFGDTAQALLPVHTSQPFAYAMVGMGAFLAAA 360 LAT+ITVGSGAVGGIFTPTLFVGAAVGYLFGD AQALLPVH SQPFAYAMVGMGAFLAAA Sbjct: 301 LATSITVGSGAVGGIFTPTLFVGAAVGYLFGDAAQALLPVHISQPFAYAMVGMGAFLAAA 360 Query: 361 SYAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVVARSVDGRSMYEITLKRHRDNEERLRL 420 SYAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVVARSVDGRSMYEITLKRHRDNEERLRL Sbjct: 361 SYAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVVARSVDGRSMYEITLKRHRDNEERLRL 420 Query: 421 RGTHMSELIRPAETVLMEDASLEEVSALFLKYPVKYVYIVDAQQRYQGVVALQDITSWLL 480 RGTHMSELIRPAETVL E+ASLEE+SALFLKYPVKYVYIVD +QRY GVVALQDITSWLL Sbjct: 421 RGTHMSELIRPAETVLHEEASLEEISALFLKYPVKYVYIVDGRQRYLGVVALQDITSWLL 480 Query: 481 DKKQAAGSMARDFLRPHFLHEVTPDMSLGQALQLFLDHQGERLPVIASAEDPRLLGVVFK 540 DKK+AAG +ARDFLRPHFLHEVTPDMSLG+ALQLFLDHQGERLPVIASA+DP+LLGVVFK Sbjct: 481 DKKEAAGRVARDFLRPHFLHEVTPDMSLGEALQLFLDHQGERLPVIASADDPQLLGVVFK 540 Query: 541 TSLLDAYFRLDRAGS 555 TSLLDAYFRLDRAG+ Sbjct: 541 TSLLDAYFRLDRAGN 555 >gi|300310913|ref|YP_003775005.1| chloride channel EriC protein [Herbaspirillum seropedicae SmR1] Length = 572 Score = 108 bits (270), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 144/514 (28%), Positives = 229/514 (44%), Gaps = 38/514 (7%) Query: 48 VRVLLPTCGGVVAGLFLVWAKKTPAGNG-GDYMEAVAIGDGAIPVRNTLLRSISSLASIA 106 + LLP GG++ GL + G+G + +EA+ G + + LL+ +SS I Sbjct: 65 IAALLPIAGGLIVGLMARYGSDKIRGHGIPEAIEAILFGKSRMSPKVALLKPLSSGIVIG 124 Query: 107 SGGSIGREGPMVQLSALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGAFFIT 166 SGG G EGP++ SL+ + H ++ + L+ GA AG+T+ + P+A Sbjct: 125 SGGPFGAEGPIIMTGGSIGSLLAQLLHLTAAERKTLLVAGACAGMTAIFGTPLAAVLLAV 184 Query: 167 EIVLGSLVMESFGPVMVASVVANITMRELPGYRATYEMPYFP-EIGG---WEVLLFLVLG 222 E++L L S PV +A VA L P FP ++ +L LV G Sbjct: 185 ELLLFELKPRSLLPVALACAVAGFVRPWL-----VEAGPLFPLDVAAPTPVALLSCLVAG 239 Query: 223 VLAGILAPQFLRVLELGKHGFAKFKLPLPVRLGVGGLLVGLISIQVPEVWGNGYSLVNSL 282 + +G+LA L + GF + +L +GG+ VGL + P G GY ++ L Sbjct: 240 LASGVLAAALTLALYRIEDGFHRLRLHWMWWPALGGIAVGLGGLLQPRALGVGYDVIGDL 299 Query: 283 LHTTWLWQMVLMVLVIKVLATAITVGSGAVGGIFTPTLFVGAAVGYLFGDTAQALLPVHT 342 LH L L +L++K + + +GSG GG+ P L +GA +G LP + Sbjct: 300 LHQHMLLSAALALLLVKAVIWLLALGSGTSGGVLAPLLMLGAGLGL----VLSPWLPGGS 355 Query: 343 SQPFAYAMVGMGAFLAAASYAPLMAILMIFEMTLSYQVVLPLMLSCVVAY-----VVARS 397 + +V M L + APL AI+ F +T + Q +LPL+ + VAY ++ RS Sbjct: 356 DG--LWPLVCMAGVLGSVLGAPLTAIVFAFGLTHASQALLPLIATTAVAYGVNVLLMKRS 413 Query: 398 VDGRSMYEITLKRHR----DNEERLRLRGTHMSELIRPA-ETVLMEDASLEEVSALFLKY 452 + + L HR D ER+ H+ EL+ A +T+ + E ++ + Sbjct: 414 IMTEKIARRGLHVHREYGVDPLERM-----HVEELMTAAPQTLAGALPAAEALAWCRAQE 468 Query: 453 PVKYVYIVDAQQRYQGVVALQDITSWLLDKKQAAGSMARDFLRPHFLHEVTPDMSLGQAL 512 V Y V G++AL+ + + A S D L H + P MS A Sbjct: 469 HVHRYYPVTDGVHLVGMLALRAL-------EHADASQPCDTLAQGHEHYLLPHMSARSAA 521 Query: 513 QLFLDHQGERLPVIASAEDPRLLGVVFKTSLLDA 546 + RLPV+A E +L+GV+ + L A Sbjct: 522 GMMARLGVARLPVVAGPERRKLVGVLSLSDLARA 555 >gi|300310046|ref|YP_003774138.1| chloride-channel protein [Herbaspirillum seropedicae SmR1] Length = 441 Score = 75.1 bits (183), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 112/403 (27%), Positives = 176/403 (43%), Gaps = 42/403 (10%) Query: 35 GSFVDIAKGLPWHVRVLLPTCGGVVAGLFLVWAKKTPAGNGGDYMEAVAIGDGAIPVRNT 94 G F I PW +L P V A + + P G +A+A G R + Sbjct: 58 GVFRSIQHRSPWLPLLLTPA---VCAACVWLTRRYFPGSEGSGIPQAIATLHGDTAERGS 114 Query: 95 LLRSIS--------SLASIASGGSIGREGPMVQL-SALCASLVGKFSHFPSSRLRLLVAC 145 L S+ S+ +IA G +IGREGP VQ+ +A+ +L + R L+ Sbjct: 115 TLLSLRVLLGKIVLSVVAIAGGMTIGREGPTVQIGAAIMYNLRRLYPAGYEHMERRLILA 174 Query: 146 GAAAGITSAYNAPIAGAFFITEIVLGSLVMESFGPVMVASVVANITMRELPGYRATYEMP 205 GAAAG+ +A+N P+AG F E + S + G V+ + A + + G + Sbjct: 175 GAAAGLAAAFNTPLAGIVFAIEELSRSFEQRASGVVITTIIFAGLVALSINGNYTYFGSI 234 Query: 206 YFP-EIGGWEVLLFLVLGVLAGILAPQF----LRVLELGKHGFAKFKLPLPVRL-GVGGL 259 FP E G +L +V VL G+ F L + PV V G Sbjct: 235 AFPGETSGRLILAVIVTAVLMGVAGGLFCWLILNPTRWIPPALVTLRTERPVAFAAVCGF 294 Query: 260 LVGLISIQV-PEVWGNGYSLVNSLLHTTWLWQMVLMVLVIKVLATAITVGS---GAVGGI 315 +V +I + +G+GY + LL+ + + L A +GS G GGI Sbjct: 295 IVAVIGVAAGGATFGSGYEQAHGLLNDGQ-----GLSVFYPFLKFASMIGSYLPGLPGGI 349 Query: 316 FTPTLFVGAAVG----YLFGDTAQALLPVHTSQPFAYAMVGMGAFLAAASYAPLMAILMI 371 F P+L +GA G LFG T+ +L A AMVG +LAA + +P+ A +++ Sbjct: 350 FAPSLSIGAGFGNLLHLLFGGTSLPML-------VALAMVG---YLAAVTQSPITAFVIV 399 Query: 372 FEMTLSYQVVLPLMLSCVVAYVVARSVDGRSMYEITLKRHRDN 414 EM + +V+ LM + ++A V++ V +YE +R+ + Sbjct: 400 MEMVDGHALVISLMATALIASAVSK-VFAPPLYETLAERYLKS 441 >gi|300311782|ref|YP_003775874.1| chloride channel protein [Herbaspirillum seropedicae SmR1] Length = 442 Score = 70.5 bits (171), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 98/359 (27%), Positives = 152/359 (42%), Gaps = 38/359 (10%) Query: 51 LLPTCGGVVAGLFLVWAKKTPAGNGGDYMEAVAIGDGAIPVRNTLLRSISSLASIASGGS 110 LLP G V L+L K AGN ++ + IP+R L + ++AS G S Sbjct: 57 LLPLGGFAVGWLYLHTGKPVEAGNN-LLIDEIHDPRKVIPLRMAPLVLLGTVASHLFGAS 115 Query: 111 IGREGPMVQLSALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGAFFITEIV- 169 +GREG VQ+ A + R+L+ G +AG +S + P+AGA F E++ Sbjct: 116 VGREGTAVQMGGALADQLTHLLRLRPEDRRILLMAGISAGFSSVFGTPMAGAIFGLEVLA 175 Query: 170 LGSLVMESFGPVMVASVVANITMRELPGYRATYEMPYFPEIGGWEVLLFLVLGV---LAG 226 +G + E+ P VA++ A+ + Y M + W V + GV LAG Sbjct: 176 IGRMRYEAIFPCFVAAIAADQVGLAWGVHHTHYAMNASAPMALWSVAAVIAAGVVFGLAG 235 Query: 227 ILAPQFLRVLELGKHGFAKFKLPL-PVRLGVGGLLVGLISIQVPEVWGNGYS-------- 277 ++ L F K + P+R +GG +V L VW G Sbjct: 236 MIFANSTHTLS----AFMKRHVSYAPLRPFIGGFIVAL------AVWAVGTHRYIGLGIP 285 Query: 278 -LVNSLLHTTWLWQMVLMVLVIKVLATAITVGSGAVGGIFTPTLFVGAAVGYLFGDTAQA 336 +V + W + K++ T ++GSG GG TP ++GA +G A Sbjct: 286 VIVEAFQQPLAPWD-----FLGKMVFTVTSLGSGFKGGEVTPLFYIGATLG-------NA 333 Query: 337 LLPVHTSQPFAYAMVGMGAFLAAASYAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVVA 395 L P+ A +G A A A+ PL + +M E+ Q+ L+CVVAY+ + Sbjct: 334 LAPLLHLPFPLLAGIGFVAVFAGAANTPLASTIMAIEL-FGPQIGPFAALACVVAYLFS 391 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.140 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,612,505 Number of extensions: 64599 Number of successful extensions: 304 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 293 Number of HSP's successfully gapped: 4 Length of query: 555 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 465 Effective length of database: 1,193,720 Effective search space: 555079800 Effective search space used: 555079800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)