BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1945.p (409 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Her... 842 0.0 gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic ami... 113 3e-26 gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic ami... 98 1e-21 gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent a... 91 2e-19 gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotr... 84 2e-17 >gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 409 Score = 842 bits (2175), Expect = 0.0, Method: Compositional matrix adjust. Identities = 402/409 (98%), Positives = 407/409 (99%) Query: 1 VRPIQKSKKLADVCYDIRGPVLEKARQMEEEGHKIIKLNIGNLAAFGFDAPDEIVQDMIR 60 +RPIQKSKKLADVCYDIRGPVLEKARQMEEEGHKIIKLNIGNLAAFGFDAPDEIVQDMIR Sbjct: 1 MRPIQKSKKLADVCYDIRGPVLEKARQMEEEGHKIIKLNIGNLAAFGFDAPDEIVQDMIR 60 Query: 61 NMGNASGYTDSKGLFAPRKSVMHYTQQKKIEGVTIDDVYLGNGASELIVMSVNALLNTGD 120 NMGNASGYTDSKGLFAPRKSVMHYTQQKKIEGVTIDD+YLGNGASELIVMSVNALLNTGD Sbjct: 61 NMGNASGYTDSKGLFAPRKSVMHYTQQKKIEGVTIDDIYLGNGASELIVMSVNALLNTGD 120 Query: 121 EVLVPSPDYPLWTAAVSLSGGTPVHYVCDEQQGWQPDIADIKKKITPNTKAIVVINPNNP 180 EVLVPSPDYPLWTAAVSLSGGTPVHYVCDEQQGWQPDIADIKKKITPNTKAIVVINPNNP Sbjct: 121 EVLVPSPDYPLWTAAVSLSGGTPVHYVCDEQQGWQPDIADIKKKITPNTKAIVVINPNNP 180 Query: 181 TGALYSVEVLKEIIELARQHQLIILADEIYDKVLYDGNTHTSLASLADDVLFITFNGLSK 240 TGALYSVEVLKEIIELARQHQLIILADEIYDKVLYDGNTHTSLASLADDVLFITFNGLSK Sbjct: 181 TGALYSVEVLKEIIELARQHQLIILADEIYDKVLYDGNTHTSLASLADDVLFITFNGLSK 240 Query: 241 NYRSCGYRAGWMVVSGEKKHARDYIEGLNMLASMRLCANAPGQFAIQTALGGYQSINDLV 300 NYRSCGYRAGWMVVSGEKKHARDYIEGLNMLASMRLCANAPGQFAIQTALGGYQSINDLV Sbjct: 241 NYRSCGYRAGWMVVSGEKKHARDYIEGLNMLASMRLCANAPGQFAIQTALGGYQSINDLV 300 Query: 301 APNGRLTKQRDLAHKLLTDIPGVTCVKPKSALYMFPRLDPEIYPIKDDQEFAYELLAEEK 360 P GRLTKQRDLAH+LLTDIPGV+CVKPKSALYMFPRLDPEIYPIKDDQEFAYELLAEEK Sbjct: 301 GPQGRLTKQRDLAHQLLTDIPGVSCVKPKSALYMFPRLDPEIYPIKDDQEFAYELLAEEK 360 Query: 361 VLIVQGTGFNCPTPDHFRVVFLPNTDDLSESMGRIAHFLEGYRRRHGTA 409 VLIVQGTGFNCPTPDHFRVVFLPNTDDL+ESMGRIAHFLEGYRRRHGTA Sbjct: 361 VLIVQGTGFNCPTPDHFRVVFLPNTDDLTESMGRIAHFLEGYRRRHGTA 409 >gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 431 Score = 113 bits (282), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 98/390 (25%), Positives = 183/390 (46%), Gaps = 21/390 (5%) Query: 8 KKLADVCYDIRGPVLEKARQMEEEGHKIIKLNIGNLAAFGFDAPDEIVQDMIR--NMGNA 65 ++L ++I + AR+ G II +++GN P IV+ ++ + Sbjct: 45 ERLPPYVFNITAELKMAARR---RGEDIIDMSMGNPDG---ATPPHIVEKLVEVAQRPDT 98 Query: 66 SGYTDSKGLFAPRKSVMH-YTQQKKIEGVTIDDVYLGNGASELIVMSVNALLNTGDEVLV 124 GY+ SKG+ R+++ H Y + +++ + + G+ E + + A L+ GD VLV Sbjct: 99 HGYSSSKGIPRLRRAIAHWYRSRYEVDFDPDSEAIVTIGSKEGLAHLMLATLDKGDTVLV 158 Query: 125 PSPDYPLWTAAVSLSGGTPVHYVCDEQQGWQPDIADIKKKITPNTKAIVVINPNNPTGAL 184 P+P YP+ ++G + ++ ++ P K +++ P+NPT Sbjct: 159 PNPSYPIHIYGAVIAGANIRSVRMSPGVDFFDELERAVRESYPKPKMMILGFPSNPTAQC 218 Query: 185 YSVEVLKEIIELARQHQLIILADEIYDKVLYDGNTHTSLASL--ADDVLFITFNGLSKNY 242 +E + +++LAR+HQ++++ D Y + +DG S+ + A +V + F LSK+Y Sbjct: 219 VELEFFERVVKLAREHQILVVHDLAYADITFDGWKAPSIMQVPGAREVA-VEFFTLSKSY 277 Query: 243 RSCGYRAGWMVVSGEKKHARDYIEGLNMLASMRLCANAPGQFAIQTALGGYQSINDLVAP 302 G+R G+MV + A I+ + S P Q A AL G QS + + Sbjct: 278 NMAGWRIGFMVGNARLVAALARIKSYHDYGSF-----TPVQVAAIAALEGDQSCVEEIRA 332 Query: 303 NGRLTKQRDLAHKLLTDIPGVTCVKPKSALYMFPRLDPEIYPIKDDQEFAYELLAEEKVL 362 N ++R++ K L + G PK+++Y++ R+ PE Y EFA LL + KV Sbjct: 333 N--YERRRNVLVKGLHEA-GWMVDVPKASMYIWARI-PEPYRQFGSLEFARILLEQAKVC 388 Query: 363 IVQGTGFNCPTPDHFRVVFLPNTDDLSESM 392 + G GF ++ R + N + +++ Sbjct: 389 VSPGIGFGEYGDEYVRFALIENESRIRQAI 418 >gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 393 Score = 98.2 bits (243), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 98/387 (25%), Positives = 177/387 (45%), Gaps = 23/387 (5%) Query: 23 EKARQMEEEGHKIIKLNIGNLAAFGFDAPDEIVQDMIRNMGNAS-GYTDSKGLFAPRKSV 81 +KA +E++G +I + IG F AP +V+ R M YT + GL R ++ Sbjct: 23 KKAAVLEQQGRHLIHMGIGEP---DFTAPPAVVEAATRAMTEGKMQYTSATGLPQLRAAI 79 Query: 82 M-HYTQQKKIEGVTIDDVYLGNGASELIVMSVNALLNTGDEVLVPSPDYPLWTAAVSLSG 140 HY +E + + + + GAS ++++ AL+ EVL+P P YP V+ Sbjct: 80 SDHYRSVYGLE-IAPERIVVTAGASAALLLACAALVERDSEVLMPDPSYPCNRHFVAAFE 138 Query: 141 GTPVHYVCDEQQGWQPDIADIKKKITPNTKAIVVINPNNPTGALYSVEVLKEIIELARQH 200 G + +Q +++ T+ +++ +P+NPTG + L+ I+ RQ Sbjct: 139 GRAKLIASGPEHRFQLSAQMVREHWGSATRGVLLASPSNPTGTSILPDELRAIVGEVRQR 198 Query: 201 QLIILADEIYDKVLYDGNTHTSLASLADDVLFITFNGLSKNYRSCGYRAGWMVVSGEKKH 260 + DEIY + Y+G ++L SL DDV+ I N SK + G+R GW+V+ Sbjct: 199 GGFTIVDEIYQGLSYEGAPFSAL-SLGDDVVVI--NSFSKYFNMTGWRLGWLVLPPA--- 252 Query: 261 ARDYIEGLNMLA-SMRLCANAPGQFAIQTALGGYQSINDLVAPNGRLTKQRDLAHKLLTD 319 + + LA ++ +CA++ Q A A Q++ ++RD L Sbjct: 253 ---LVPQVEKLAQNLLICASSIAQHA-AVACFTPQTLALYEERKAEFKRRRDYIVPALES 308 Query: 320 IPGVTCVKPKSALYMFPRLDPEIYPIKDDQ-EFAYELLAEEKVLIVQGTGFNCPTPDHF- 377 + V P A Y++ + + DD + + ++L E V++V G F T + Sbjct: 309 LGFTVPVMPDGAFYVY----ADCSALADDADQLSLDMLNEAGVVLVPGLDFGPFTARRYI 364 Query: 378 RVVFLPNTDDLSESMGRIAHFLEGYRR 404 R+ + + ++L E++ R+ F E R+ Sbjct: 365 RLSYATSMENLQEAVARLRVFFEKRRK 391 >gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 90.9 bits (224), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 54/163 (33%), Positives = 93/163 (57%), Gaps = 8/163 (4%) Query: 95 IDDVYLGNGASELIVMSVNALLNTGDEVLVPSPDYPLWTAAVSLSGGTPVHYVCDE--QQ 152 + ++ + GA++ I+ SV ++ GDEV+V P Y + A+ L+GG PV +V E Q Sbjct: 89 VSEITVTAGATQGILTSVLCAVHPGDEVIVIEPVYDCYVPAIELAGGVPV-FVQMEVGAQ 147 Query: 153 GWQPDIADIKKKITPNTKAIVVINPNNPTGALYSVEVLKEIIELARQHQLIILADEIYDK 212 G+ +K +TP T+ I+V P+NPTG++ + + ++ R ++IL+DE+Y+ Sbjct: 148 GYSIPWDKVKAAVTPKTRMIMVNTPHNPTGSVMRAADVAALADIVRGTDILILSDEVYEH 207 Query: 213 VLYDGNTHTSLA---SLADDVLFITFNGLSKNYRSCGYRAGWM 252 ++YDG H SLA LA+ FI + K Y G++ G++ Sbjct: 208 MVYDGQPHESLARHPELAERS-FIN-SSFGKTYHVTGWKVGYV 248 >gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 420 Score = 84.3 bits (207), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 85/389 (21%), Positives = 170/389 (43%), Gaps = 39/389 (10%) Query: 30 EEGHKIIKLNIGNLAAFGFDAPDEIVQDMIRNMGNASGYTDSKGLFAPRKSVMHYTQQK- 88 + ++ I L IG P I Q +I N+ + Y + G A R ++ + Q++ Sbjct: 43 DPAYRPISLGIGEPK---HPTPAFIQQALIDNLAGLASYPATLGSDALRGAIADWLQKRY 99 Query: 89 ---KIEGVTIDDVYLGNGASELIVMSVNALLNT---GDEVLVPSPDYPLWTAAVSLSGGT 142 + + T ++ NG+ E + +++ G V+ P+P Y ++ + L+G Sbjct: 100 DIPRPDPAT--EILPVNGSREALFSLTQTVVDPTRPGALVMCPNPFYQIYEGSAYLAGAQ 157 Query: 143 PVHYVCDEQQGWQPDIADIKKKITPNTKAIVVINPNNPTGALYSVEVLKEIIELARQHQL 202 P D ++ + PD + + + + + + V +P NPTGA+ ++E KE+ L+ ++ Sbjct: 158 PYFVNSDPKRNFAPDFSQVPQDVWERVQLLFVCSPGNPTGAVLTLEDWKELFALSDRYGF 217 Query: 203 IILADEIYDKVLYD-----GNTHTSLASLADDVLFITFNGLSKNYRSCGYRAGWMVVSGE 257 +I +DE Y ++ ++ G + D I+F+ LSK G R+G+ V+G+ Sbjct: 218 VIASDECYSEIYFNEDPPLGGMQAARLLGRDYRRLISFSSLSKRSNVPGMRSGF--VAGD 275 Query: 258 KKHARDYIEGLNMLASMRLCANAPGQFAIQTA-LGGYQSINDLVAPNGRLTKQRDLAHKL 316 + ++ + + A +P IQ A + +Q + + + L Sbjct: 276 AAILKKFL----LYRTYHGAAMSP---VIQAASVLAWQDETHVQGNIAKYVTKFSQVTPL 328 Query: 317 LTDIPGVTCVKPKSALYMFPRLDPEIYPIKDDQEFAYELLAEEKVLIVQGT-------GF 369 L ++ + P +A Y++ ++D + D EFA L AE V ++ G+ G Sbjct: 329 LQEV--LEVALPDAAFYLWAKVDKLVN--ISDTEFAQRLYAEYNVTVLPGSYLAREAHGV 384 Query: 370 NCPTPDHFRVVFLPNTDDLSESMGRIAHF 398 N P R+ + ++ E+ RI F Sbjct: 385 N-PGAGRIRMALVAEVEECLEAAQRIVAF 412 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,303,437 Number of extensions: 53754 Number of successful extensions: 172 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 167 Number of HSP's successfully gapped: 6 Length of query: 409 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 322 Effective length of database: 1,207,925 Effective search space: 388951850 Effective search space used: 388951850 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)