BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1882.p (359 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylester... 681 0.0 gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylester... 263 2e-71 gi|300311029|ref|YP_003775121.1| protein-glutamate methylesteras... 235 3e-63 gi|300312321|ref|YP_003776413.1| protein-glutamate methylesteras... 155 7e-39 gi|300312675|ref|YP_003776767.1| protein-glutamate methylesteras... 94 1e-20 >gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 681 bits (1756), Expect = 0.0, Method: Compositional matrix adjust. Identities = 352/359 (98%), Positives = 354/359 (98%), Gaps = 1/359 (0%) Query: 2 MKIKVLIVDDSALIRSVMREIIGSQPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPR 61 MKIKVLIVDDSALIRSVMREIIGSQPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPR Sbjct: 1 MKIKVLIVDDSALIRSVMREIIGSQPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPR 60 Query: 62 MDGLDFLEKLMRLRPMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQEYAD 121 MDGLDFLEKLMRLRPMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQEYAD Sbjct: 61 MDGLDFLEKLMRLRPMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQEYAD 120 Query: 122 MIADKIRAAAKARVRARPSRSAEQATTSG-EVLPQIRNPLTSSEKLIIIGASTGGTEAIK 180 MIADKIRAAAKARVRARP+RSAEQA G E LPQIRNPLTSSEKLIIIGASTGGTEAIK Sbjct: 121 MIADKIRAAAKARVRARPARSAEQAAGGGSEALPQIRNPLTSSEKLIIIGASTGGTEAIK 180 Query: 181 EFLIRMPSDCPGILITQHMPEGFTRSFAQRLNNLCKISVVESAGNERVLPGHAYIAPGHS 240 EFLIRMPSDCPGILITQHMPEGFTRSFAQRLNNLCKISVVESAGNERVLPGHAYIAPGHS Sbjct: 181 EFLIRMPSDCPGILITQHMPEGFTRSFAQRLNNLCKISVVESAGNERVLPGHAYIAPGHS 240 Query: 241 HLKLVRSGANYMTQLDQGPPVNRHRPSVDVLFQSAATCAGKNAVGVILTGMGKDGAQGML 300 HLKLVRSGANYMTQLDQGPPVNRHRPSVDVLFQSAATCAGKNAVGVILTGMGKDGAQGML Sbjct: 241 HLKLVRSGANYMTQLDQGPPVNRHRPSVDVLFQSAATCAGKNAVGVILTGMGKDGAQGML 300 Query: 301 EMRNAGAYNFAQDEASCVVFGMPREAIAVGAAHEVLSLQAMPGRVLAWLAEHGSRALRV 359 EMRNAGAYNFAQDEASCVVFGMPREAIAVGAAHEVL+LQAMPGRVLAWLAEHGSRALRV Sbjct: 301 EMRNAGAYNFAQDEASCVVFGMPREAIAVGAAHEVLALQAMPGRVLAWLAEHGSRALRV 359 >gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 381 Score = 263 bits (671), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 144/351 (41%), Positives = 215/351 (61%), Gaps = 27/351 (7%) Query: 16 RSVMREIIGSQPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPRMDGLDFLEKLMRLR 75 R +M++++ PD+ V+G APDP+ A ++Q PDV+ LD++MPRMDG+ FL ++M R Sbjct: 19 RQLMQQVLAQTPDIRVIGTAPDPVFALRKMQQQWPDVIVLDIKMPRMDGVSFLRQIMATR 78 Query: 76 PMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQEYADMIADKIRAAAKARV 135 P PV+M S+ + + AL GA+ + +P++ ++ +Q+ + +A IRAA +AR Sbjct: 79 PTPVIMCSAHVGPDNALAAEALAAGAVSVIARPQLGVRDFLQQAVEELASAIRAAMQARE 138 Query: 136 RAR----PSR--------------------SAEQATTSGEVL--PQIRNPLT-SSEKLII 168 R P+R + Q T+ VL P + L ++ +++ Sbjct: 139 GQRAPKAPARLGLSPVKAGAREMLHDAAWGAVLQRYTADAVLQPPGPEDGLRPATARIVA 198 Query: 169 IGASTGGTEAIKEFLIRMPSDCPGILITQHMPEGFTRSFAQRLNNLCKISVVESAGNERV 228 IGASTGG +A++ L ++ CPGI++ QHMP+ FTRSFA+RL+ + V E+ +R+ Sbjct: 199 IGASTGGPQALERVLGKLSQRCPGIVVVQHMPQRFTRSFAERLHRISGAEVKEAEHLDRI 258 Query: 229 LPGHAYIAPGHSHLKLVRSGANYMTQLDQGPPVNRHRPSVDVLFQSAATCAGKNAVGVIL 288 LPG IAPG HL + R G Y + GP V+RH+PSVDVLF+S A AG NAVG+I+ Sbjct: 259 LPGRVLIAPGGRHLVVRREGLQYYVETLDGPLVSRHKPSVDVLFRSLAKAAGSNAVGIIM 318 Query: 289 TGMGKDGAQGMLEMRNAGAYNFAQDEASCVVFGMPREAIAVGAAHEVLSLQ 339 TGMG DGA+GM E+ + G +AQDEAS VVFGMP+EAI G +V++L+ Sbjct: 319 TGMGDDGARGMRELADCGGATYAQDEASSVVFGMPKEAIQQGGVGDVITLE 369 >gi|300311029|ref|YP_003775121.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 353 Score = 235 bits (600), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 138/353 (39%), Positives = 205/353 (58%), Gaps = 6/353 (1%) Query: 4 IKVLIVDDSALIRSVMREIIGSQPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPRMD 63 IK+LI+DDSAL+R + ++ ++ + + A EL + PDV+TLD+ MP MD Sbjct: 2 IKLLIIDDSALMRRQLMQVFEQAGGFDIC-LGRNGREAVELNRSFAPDVVTLDINMPEMD 60 Query: 64 GLDFLEKLMRLRPMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQEYADMI 123 GL L +M RP+PVVMVSSLTE+G+ T A+ LGA+D+V KP +I + E + Sbjct: 61 GLSALSLMMLDRPVPVVMVSSLTEQGALATFEAMNLGAVDYVAKPSGTISLSLHEIEQDL 120 Query: 124 ADKIRAAAKARVRAR---PSRSAEQATTSGEVLPQIRNPLTSSEKLIIIGASTGGTEAIK 180 K+R AAKAR+R+R P + + + V R +S+ L++IG STGG ++ Sbjct: 121 LAKVRVAAKARIRSRAPAPELARAREPLAAPVAEAARRGRPASDGLVLIGVSTGGPSTLE 180 Query: 181 EFLIRMPSDCPG-ILITQHMPEGFTRSFAQRLNNLCKISVVESAGNERVLPGHAYIAPGH 239 E L +P+ P IL+ QHMP FTRSFA+R++ LC ++V+E A V GH YIA G Sbjct: 181 EILPALPASFPWPILVAQHMPAAFTRSFAERMDKLCALNVLEVAAPTVVQGGHVYIARGG 240 Query: 240 SHLKLVRSGANYMTQLDQGPPVNRHRPSVDVLFQSA-ATCAGKNAVGVILTGMGKDGAQG 298 + + + + G++ P + PSV++L +SA + A + +GV+LTGMG DGA Sbjct: 241 ADMLVAKRGSHLSVLPKPESPTHLWHPSVELLARSALQSMAARQLIGVMLTGMGNDGAAA 300 Query: 299 MLEMRNAGAYNFAQDEASCVVFGMPREAIAVGAAHEVLSLQAMPGRVLAWLAE 351 E+ G A+ E+SCVVFGMP++ I G A VL + ++ WL + Sbjct: 301 FAEICGDGGRTIAESESSCVVFGMPKDLIDRGGASVVLPASKIATQLRVWLEQ 353 >gi|300312321|ref|YP_003776413.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 155 bits (391), Expect = 7e-39, Method: Compositional matrix adjust. Identities = 107/349 (30%), Positives = 180/349 (51%), Gaps = 15/349 (4%) Query: 4 IKVLIVDDSALIRSVMREIIGSQPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPRMD 63 +K+ IV+D+ ++ V+R +I E++ +A D A ++ PDV+ +D+ MP++D Sbjct: 1 MKIGIVNDTPMMVEVLRRVIAESGRHELIWIARDGEQAVQMCAWQLPDVVLMDLLMPKVD 60 Query: 64 GLDFLEKLMRLRPMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQEYADMI 123 G + ++M P P+++V+S + ++ A+ GA+D P + +G ++ A +I Sbjct: 61 GAEATRRIMEATPCPILVVTSDMDTSADKIYEAMGYGALDATQTPALIGAAGKRDAAALI 120 Query: 124 ADKIRAAAK--ARVRARPSRSAEQATTSGEVLPQIRNPLTSSEKLIIIGASTGGTEAIKE 181 +KI + + + P R T + P S L+ +GAS GG A+ Sbjct: 121 -EKIDNLGRLSPEMVSPPVRGGRAETLA----PAQPARADSLPPLVAVGASAGGPAALAT 175 Query: 182 FLIRMPSDCPG-ILITQHMPEGFTRSFAQRLNNLCKISVVESAGNERVLPGHAYIAPGHS 240 L +P G ++I QH+ F AQ L + V + + G +A + Sbjct: 176 LLKAIPQGFAGAVVIVQHIDAAFAPGMAQWLQQQSALKVGLAQEGQAPQRGAVLLAASNR 235 Query: 241 HLKLVRSGA--NYMTQLDQGPPVNRHRPSVDVLFQSAATCAGKNAVGVILTGMGKDGAQG 298 HL +V+ G Y+ +G P + +RPSVDV FQS G++LTGMG+DGA G Sbjct: 236 HL-VVKEGGRLGYV----EGAPNDVYRPSVDVFFQSIVRHWPGKVAGLLLTGMGRDGAAG 290 Query: 299 MLEMRNAGAYNFAQDEASCVVFGMPREAIAVGAAHEVLSLQAMPGRVLA 347 + +R++GA+ AQD+ SC V+GMP+ A+A+ AA EVL + M R+ A Sbjct: 291 LKALRDSGAHTMAQDQKSCAVYGMPKAAVALNAAVEVLPVTGMAARLQA 339 >gi|300312675|ref|YP_003776767.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 211 Score = 94.4 bits (233), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 61/174 (35%), Positives = 85/174 (48%), Gaps = 1/174 (0%) Query: 167 IIIGASTGGTEAIKEFLIRMPSDCP-GILITQHMPEGFTRSFAQRLNNLCKISVVESAGN 225 I+IGAS GG EA+ E L + ++ H+P AQ + C+ VVE+ Sbjct: 17 IVIGASAGGVEALSEILPALRRGAAVATMVVLHLPRERPSLLAQIFADRCRQPVVEAQDK 76 Query: 226 ERVLPGHAYIAPGHSHLKLVRSGANYMTQLDQGPPVNRHRPSVDVLFQSAATCAGKNAVG 285 E G + AP HL + RS + + VN RP++DVLF+SAA G +G Sbjct: 77 EPAQAGVIHFAPPDYHLLVDRSEDGPILAMSNDELVNYSRPAIDVLFESAADVYGPRLLG 136 Query: 286 VILTGMGKDGAQGMLEMRNAGAYNFAQDEASCVVFGMPREAIAVGAAHEVLSLQ 339 ++LTG DGA GM + AG Q V MP +A+A+G VL+LQ Sbjct: 137 ILLTGGNHDGAAGMQAIARAGGVTVVQSPQQAQVPYMPAQALAMGNIDYVLTLQ 190 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,061,300 Number of extensions: 42295 Number of successful extensions: 241 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 242 Number of HSP's successfully gapped: 14 Length of query: 359 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 273 Effective length of database: 1,212,660 Effective search space: 331056180 Effective search space used: 331056180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)