BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1857.p (680 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311314|ref|YP_003775406.1| O-antigen acetylase [Herbaspiri... 989 0.0 gi|300310314|ref|YP_003774406.1| acyltransferase [Herbaspirillum... 420 e-118 gi|300311522|ref|YP_003775614.1| O-antigen acetylase [Herbaspiri... 416 e-117 gi|300310014|ref|YP_003774106.1| o-antigen acetylase [Herbaspiri... 404 e-113 >gi|300311314|ref|YP_003775406.1| O-antigen acetylase [Herbaspirillum seropedicae SmR1] Length = 662 Score = 989 bits (2558), Expect = 0.0, Method: Compositional matrix adjust. Identities = 472/650 (72%), Positives = 542/650 (83%) Query: 28 AVPQLHPKYRPDIDGLRAIAVASVVIFHAFPEWFKGGFIGVDIFFVISGFLISLIIFNNL 87 A P LHP YRPDIDGLRA+AV SVVIFHAFPEWF GGFIGVDIFFVISGFLISLI+F NL Sbjct: 11 AAPSLHPAYRPDIDGLRAVAVGSVVIFHAFPEWFAGGFIGVDIFFVISGFLISLILFKNL 70 Query: 88 EHDRFSILDFYVRRVKRIFPALMTVMIVCAVAGAAILFADEYRQLGKHLLGGSTFISNIL 147 EH RFSI+DFYVRRV+RIFPALMTVM +C V G +LFADEY+QLGKHL GGSTFISN L Sbjct: 71 EHGRFSIVDFYVRRVRRIFPALMTVMSLCLVTGWFVLFADEYKQLGKHLFGGSTFISNFL 130 Query: 148 FWRESGYFDNSAETKPLLHLWSLAIEEQFYLFWPLLLALVWKRKWGFLRIIAVIALASFA 207 FWRESGYFDNSAETKPLLHLWSLAIEEQFYLFWPLLLA VWKR+WGFL I+ VI L SF Sbjct: 131 FWRESGYFDNSAETKPLLHLWSLAIEEQFYLFWPLLLAFVWKRRWGFLGIMGVIGLLSFG 190 Query: 208 VNLYLVGNDQTAAFYSPFARFWELMVGGTLGYISLHRADLNARFKNLQSWLGVLLLAIGF 267 VNLY +G+DQ+AAFYSP ARFWELM+GG L YI+LHR DL +KNLQ+WLG LL GF Sbjct: 191 VNLYFIGHDQSAAFYSPLARFWELMIGGALAYINLHRQDLTRGYKNLQAWLGAALLVAGF 250 Query: 268 VLLNGTRSFPGWWALLPCIGAFLLLSAGPAAWFNSKVLASKPMVWIGLISYPLYLWHWPL 327 LL+G R+FPGWWALLP +GAFLLLSAGP AWFN +VLASK MV +GL+SYPLYLWHWPL Sbjct: 251 ALLDGARAFPGWWALLPSLGAFLLLSAGPGAWFNRRVLASKGMVALGLVSYPLYLWHWPL 310 Query: 328 LAFATIIAGQTAPQEWRLLAVVAAVLLAWGTFLFIERPLRHAGSVKIVLVLVALNIVVGA 387 LAFATII G++AP EWR AV+ A+LL+W T+ +IERPLR + + VLVL+A+N+ VG Sbjct: 311 LAFATIIEGESAPAEWRAAAVLGALLLSWLTYRYIERPLRGSTRSRTVLVLLAINVAVGL 370 Query: 388 AGMVIYRNGGFPQRAINQKLDYLARSDVFEGSRNSDGSCARLNHLTEVSEEVCLSNSAQP 447 G+ IY GGF +RAIN KLDYL +SDVFEGSRNSDGSCAR N + V EEVCLSNSA P Sbjct: 371 GGLAIYLKGGFDERAINGKLDYLTQSDVFEGSRNSDGSCARFNGMEGVDEEVCLSNSAAP 430 Query: 448 QVLFVGDSHVMALHSAIFSRQLALPSIMVAGHACRVYPNLPYEPANVRRWSNNCTEIAQQ 507 + LFVGDSHVMALHSAIFS + +P++MVAGHACRVYPNLPY P ++ RW+NNCT IA + Sbjct: 431 RTLFVGDSHVMALHSAIFSGDVKMPAMMVAGHACRVYPNLPYTPTHLLRWANNCTAIANE 490 Query: 508 AIAVGQKFPSIQTVVISGAYPRGNLDTPSQYKVNGSSLSEREAFMSGYGALIESFIAQGK 567 AIAV K PSI TVVISGAYPRG+L PS+Y+ +G +LS+REAF+SGYGALIE+F QGK Sbjct: 491 AIAVAGKLPSIDTVVISGAYPRGDLSQPSEYQQDGRALSKREAFLSGYGALIEAFRKQGK 550 Query: 568 RVVFMADVPYLKLDPHSCVSRFGDAPTRECAFTQAEHERIRKAYDAAVDELRQLHPKATV 627 +VVFM DVPYLKLDPH+CV+RF D R+CA+T+AEH+ +R+ YDAAV L++ HP A Sbjct: 551 KVVFMPDVPYLKLDPHACVNRFSDGQARDCAYTEAEHQALRRDYDAAVAALQRAHPDAVF 610 Query: 628 FDPVSLFCAQGKCQAKIDGAYLYNDDQHISLAASKYLLQSMQRDGLLPAS 677 FDP LFC GKCQAK+ AYLYND+QHISLAAS+ LL MQR GLLPA+ Sbjct: 611 FDPQGLFCPDGKCQAKLGEAYLYNDNQHISLAASRLLLAEMQRRGLLPAA 660 >gi|300310314|ref|YP_003774406.1| acyltransferase [Herbaspirillum seropedicae SmR1] Length = 656 Score = 420 bits (1079), Expect = e-118, Method: Compositional matrix adjust. Identities = 288/679 (42%), Positives = 377/679 (55%), Gaps = 65/679 (9%) Query: 5 HKNPALDSSAQAAPPAALDTTGHAVPQLHPKYRPDIDGLRAIAVASVVIFHAFPEWFKGG 64 HK PA AA PA HP YRPDIDGLRAIAV SVV+FHAFPEW GG Sbjct: 6 HKTPA-----GAASPATA----------HPGYRPDIDGLRAIAVLSVVVFHAFPEWLPGG 50 Query: 65 FIGVDIFFVISGFLISLIIFNNLEHDRFSILDFYVRRVKRIFPALMTVMIVCAVAGAAIL 124 F+GVDIFFVISGFLIS I+F+ L+ DRFSI+DFY+RR++RIFPAL+ VM+ C +AG IL Sbjct: 51 FVGVDIFFVISGFLISGILFDALQRDRFSIVDFYIRRIRRIFPALLLVMLACLLAGWFIL 110 Query: 125 FADEYRQLGKHLLGGSTFISNILFWRESGYFDNSAETKPLLHLWSLAIEEQFYLFWPLLL 184 +EYRQLGKHLLGG+ F+SNI+ W E+GYFD SAETKPLLHLWSL +EEQFYLFWPL+L Sbjct: 111 LPEEYRQLGKHLLGGAGFVSNIVLWNEAGYFDTSAETKPLLHLWSLGVEEQFYLFWPLML 170 Query: 185 ALVWKRKWGFLRIIAVIALASFAVNLYLVGNDQTAAFYSPFARFWELMVGGTLGYISLHR 244 W+R+ G L ++ + SF N+ + D AAFY P +RFWEL G L + Sbjct: 171 WAAWRRRTGMLAVLLGVMALSFGWNIVWLHRDAVAAFYLPMSRFWELGAGALLAWRHRQG 230 Query: 245 ADLNARFKNLQSWLGVLLLAIGFVLLNGTRSFPGWWALLPCIGAFLLLSAGPAAWFNSKV 304 A + + S G+LL+ + + +FPGWWALLP GA LLL+AGP A N + Sbjct: 231 AVPTSVQAHRLSVAGLLLIVVAIATITKDDAFPGWWALLPVTGAVLLLAAGPQARINRSL 290 Query: 305 LASKPMVWIGLISYPLYLWHWPLLAFATIIAGQTAPQEWRLLAVVAAVLLAWGTFLFIER 364 L+ + MVW+GLISYPLYLWHWPLLAFA ++ G T + RL AV A+LLAW T+ +E Sbjct: 291 LSHRTMVWVGLISYPLYLWHWPLLAFARLVVGATPSVQLRLAAVALALLLAWLTYRLVEL 350 Query: 365 P--LRHAGSVKIVLVLVALNIVVGAAGMVIYRNG-GFPQRAINQKL--DYLARSDVFEGS 419 P LR AG V + + + ++ G V+ RNG F Q L D + + V Sbjct: 351 PVRLRSAGHRSAVTLALLMALMAALGGYVVKRNGMDFRQLGAVAMLFNDQVKETAVLNHF 410 Query: 420 RNSDGSCARLNHLTEVSEEVCLS--NSAQPQVLFVGDSHVMA-------LHSAIFSRQLA 470 SCA L E S + C + ++ PQVL +GDS+ A L+ A QL Sbjct: 411 ELPHPSCAALMG-QEHSRDWCAAPVSAEPPQVLLIGDSYSGAYAPMLARLYQASPQPQLV 469 Query: 471 LPSIMVAGHACRVYPNLPYEPANVRRWSNNCTEIAQQAIAVGQKFPSIQTVVISGAYPRG 530 G A + Y C EI+++ A ++ PSI+TVV++ +P Sbjct: 470 FQQFARGGCASLLDGGGGY-----------CGEISERVAAYARQTPSIKTVVLAANWP-- 516 Query: 531 NLDTPSQYKVNGSSLSEREAFMSGYGALIESFIAQGKRVVFMADVPYLKLDPHSCV---S 587 G + + + I + GKRVV + P +P SCV Sbjct: 517 -----------GHIAANPKGVLKALEHTILYYRQLGKRVVVLLSPPN-GANPKSCVLRGI 564 Query: 588 RFGDAPTRECAFTQAEHERIRKAY-DAAVDELRQLHPKATVFDPVSLFCAQGKCQAKIDG 646 R DA C +A+ E++ Y D + L++L VFDP C + C+ IDG Sbjct: 565 RLSDADF--CNLPRAKAEQMDDHYRDLMLPLLQRL--DVPVFDPYPTLCDEHGCKV-IDG 619 Query: 647 A-YLYNDDQHISLAASKYL 664 LY D H+S ++YL Sbjct: 620 PRILYFDPGHLSAYGAQYL 638 >gi|300311522|ref|YP_003775614.1| O-antigen acetylase [Herbaspirillum seropedicae SmR1] Length = 680 Score = 416 bits (1069), Expect = e-117, Method: Compositional matrix adjust. Identities = 246/670 (36%), Positives = 359/670 (53%), Gaps = 51/670 (7%) Query: 33 HPKYRPDIDGLRAIAVASVVIFHAFPEWFKGGFIGVDIFFVISGFLISLIIFNNLEHDRF 92 H KYR DIDGLRAIAV +VV FH FP + +GGFIGVD+FFVISG+LIS IIF NL+ F Sbjct: 11 HSKYRSDIDGLRAIAVLAVVSFHGFPNFVRGGFIGVDVFFVISGYLISTIIFENLDRGTF 70 Query: 93 SILDFYVRRVKRIFPALMTVMIVCAVAGAAILFADEYRQLGKHLLGGSTFISNILFWRES 152 + +FY RRV+RIFPAL+ V+ C V G +L DEY+QLGKH+ GG+ F+SN L ES Sbjct: 71 TFTEFYARRVRRIFPALLLVLSTCFVVGWFVLLTDEYKQLGKHIAGGAGFVSNFLLRDES 130 Query: 153 GYFDNSAETKPLLHLWSLAIEEQFYLFWPLLLALVWKRKWGFLRIIAVIALASFAVNLYL 212 GYFDNSAETKPLLHLWSL IEEQFY+ WP+LL W+RK+ L I ++ALASFA N+ Sbjct: 131 GYFDNSAETKPLLHLWSLGIEEQFYILWPMLLFFSWRRKFNLLTITIIVALASFAANIKG 190 Query: 213 VGNDQTAAFYSPFARFWELMVGGTLGYISLHRADLNARFK-NLQSWL------------- 258 + D TAAFY P +RFWEL+ G L + +L++ + L +W+ Sbjct: 191 INKDATAAFYLPQSRFWELLSGTLLAWFTLYKQHTTIELRPRLGTWILQLIYSEKRKSDG 250 Query: 259 ----------GVLLLAIGFVLLNGTRSFPGWWALLPCIGAFLLLSAGPAAWFNSKVLASK 308 G+ ++ GF +N +FPG WAL+P +G L+++AGPAAW N +L++K Sbjct: 251 KFLIDVSSITGLCIVLYGFWRINKELAFPGVWALVPILGTILIIAAGPAAWVNRTLLSNK 310 Query: 309 PMVWIGLISYPLYLWHWPLLAFATIIAGQTAPQEWRLLAVVAAVLLAWGTFLFIERPLR- 367 +VW GLIS+PLYLWHWP+L+FA I+ + R+ A+ A++LAW T+ +ERP+R Sbjct: 311 ILVWFGLISFPLYLWHWPVLSFARIVENELPSYPLRVGAIALAIILAWLTYEIVERPIRT 370 Query: 368 --HAGSVKIVLVLVALNIVVGAAGMVIYRNGGFPQRAINQKLDYLARSDVFEGSRNSDGS 425 +A + V L+ L +VGA G + G+ R ++ + ++R+ G G Sbjct: 371 GNNANTKITVTALIGLMALVGAVGYRTFAANGWESR-MSPDITRISRA---AGEWQYPGK 426 Query: 426 CARLNHLTEVSEEVCLSNSAQPQV-LFVGDSHVMALHSAIFSRQLALPS-----IMVAGH 479 N + NS ++ LF+GDS++ + P+ + G Sbjct: 427 LTAFNF---ENRTFYRQNSNMDEITLFIGDSNIEQYYVRTDELISTKPNNSNSVVFATGG 483 Query: 480 ACRVYPNLPYEPANVRRWSNNCTEIAQQAIAVGQKFPSIQTVVISGAYPRGNLDTPSQYK 539 C P PY+ A++ +C + + A+ + S+ +VVI + D Sbjct: 484 GCLAIPGSPYDEAHM-----SCANLMETALNYAKTNASVASVVIGAQWNSYLSDGYGLRG 538 Query: 540 VNGSSLSEREAFMSGYGALIESFIAQGKRVVFMADVPY-LKLDPHSCVSRFGDAPTRECA 598 G++ + A ++ I GK+V F+ ++P +LDP ++R Sbjct: 539 SFGTAGKDYSAALNRLARYIRELKYLGKKVYFILNIPIGDELDPKFMITRNLKHFPHVIN 598 Query: 599 FTQAEHER--IRKAYDAAVDELRQL--HPKATVFDPVSLFCAQGKCQAKID-GAYLYNDD 653 + R I + Y +L Q+ A V +P+ C KC + D G +Y D Sbjct: 599 LKERGLNRKDIDERYGKIQKDLEQIARAAGADVVNPMDFLCNATKCSSLDDEGEPIYKDR 658 Query: 654 QHISLAASKY 663 H+ +Y Sbjct: 659 SHLRPQYVRY 668 >gi|300310014|ref|YP_003774106.1| o-antigen acetylase [Herbaspirillum seropedicae SmR1] Length = 681 Score = 404 bits (1039), Expect = e-113, Method: Compositional matrix adjust. Identities = 255/652 (39%), Positives = 355/652 (54%), Gaps = 45/652 (6%) Query: 35 KYRPDIDGLRAIAVASVVIFHAFPEWFKGGFIGVDIFFVISGFLISLIIFNNLEHDRFSI 94 YRPDIDGLRAIA+ SVV+FHAFP+ GGFIGVDIFFVISGFLIS IIF L+ RFS Sbjct: 31 SYRPDIDGLRAIAIVSVVVFHAFPDAMPGGFIGVDIFFVISGFLISSIIFRQLDQGRFSF 90 Query: 95 LDFYVRRVKRIFPALMTVMIVCAVAGAAILFADEYRQLGKHLLGGSTFISNILFWRESGY 154 DFYV R++RIFPAL+ V+ C V G L +EYRQLGKH+ G+ FI N++ W+ESGY Sbjct: 91 TDFYVHRIRRIFPALILVLASCFVLGWFSLLPEEYRQLGKHMAAGAGFIQNLVLWKESGY 150 Query: 155 FDNSAETKPLLHLWSLAIEEQFYLFWPLLLALVWKRKWGFLRIIAVIALASFAVNLYLVG 214 FD ++ KPLLHLWSL++EEQFYL +PLL+ +W+ + + ++ LASFA+NL+ V Sbjct: 151 FDVESDMKPLLHLWSLSVEEQFYLVYPLLVCGLWRARMPLRNSLVLLMLASFALNLWWVA 210 Query: 215 NDQTAAFYSPFARFWELMVGGTLGYISLHR--ADLNARFK-------NLQSWLGVLLLAI 265 D FY PF R WEL+ G + + + A + F+ +L+S LG+ LL Sbjct: 211 QDPVKTFYLPFTRVWELLAGALCAFGVMRKGVAFSTSSFRLPHLPSIHLRSVLGLALLLC 270 Query: 266 GFVLLNGTRSFPGWWALLPCIGAFLLLSAGPAAWFNSKVLASKPMVWIGLISYPLYLWHW 325 G VL++ R FPG WALLP GA LL+S+GP AW N +L+ +VWIGL+SY LYLWHW Sbjct: 271 GLVLIDRHRYFPGAWALLPVTGAVLLVSSGPQAWVNRHLLSRPLLVWIGLVSYLLYLWHW 330 Query: 326 PLLAFATIIAGQTAPQEWRLLAVVAAVLLAWGTFLFIERPLRHAGSVKIVLVLVALNIVV 385 P+L+F I+ L AV +VLLAW T+ +ERP+R +V L+AL + V Sbjct: 331 PILSFMHILTPTRPGAPLPLAAVALSVLLAWLTYRLLERPVRFGAKSWMVPALLALLLSV 390 Query: 386 GA-AGMVIYRNGGFPQRAINQKLDYLARSDVFEGSRNSDGSCARLNHLTEVSEEVCL-SN 443 A AG ++ G R + A +F G+ +D +C E C S Sbjct: 391 TATAGYNDFQRNGLSFRLAT----HYAEQPLFVGT--TDATCTADG---GAGLEFCRQSR 441 Query: 444 SAQPQVLFVGDSHVMALHSAIFSRQLALPSI--MVAGHACRVYPNLPYEPANVRRWSNNC 501 P ++ +GDSH L+ + S I M A AC + + A+ +R ++C Sbjct: 442 PGAPTIVVIGDSHAWHLYDGLASYAAQNGDIIGMRAVGACLGFSGV---SADTKR--HDC 496 Query: 502 TEIAQQAIAVGQKFPSIQTVVISGAYPRGNLDTPSQYKVNGSSLSEREAFMSGYGALIES 561 A+ +IA + S++TVV+S + + P E F ++ + Sbjct: 497 AP-ARDSIASAARQASVRTVVLSARLDIYDRELPG------------EVFEGALRDMLAT 543 Query: 562 FIAQGKRVVFMADVPYLKLDPHSCVS-----RFGDAPTRECAFTQAEHERIRKAYDAAVD 616 A GK+V+F+ D+P L P C+ R CA QA + + Y V+ Sbjct: 544 LTAAGKKVIFVMDIPILDFHPKYCLDISMPLRPKTTSLPNCAIQQAVFAQHTQHYRETVN 603 Query: 617 ELRQLHPKATVFDPVSLFCAQGKCQAKIDGAYLYNDDQHISLAASKYLLQSM 668 L + P+ FD + C +C A DG LY D H+S S + Q++ Sbjct: 604 ALLRQFPQVQAFDAAAPLCDHQQCWAMKDGQLLYRDYDHLSTIGSTLVAQTL 655 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.139 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,104,635 Number of extensions: 87664 Number of successful extensions: 384 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 370 Number of HSP's successfully gapped: 6 Length of query: 680 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 588 Effective length of database: 1,184,250 Effective search space: 696339000 Effective search space used: 696339000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 134 (56.2 bits)