BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1856.p (352 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311313|ref|YP_003775405.1| acyltransferase [Herbaspirillum... 379 e-106 gi|300311522|ref|YP_003775614.1| O-antigen acetylase [Herbaspiri... 92 9e-20 gi|300310014|ref|YP_003774106.1| o-antigen acetylase [Herbaspiri... 81 2e-16 gi|300311314|ref|YP_003775406.1| O-antigen acetylase [Herbaspiri... 74 2e-14 gi|300310314|ref|YP_003774406.1| acyltransferase [Herbaspirillum... 64 1e-11 >gi|300311313|ref|YP_003775405.1| acyltransferase [Herbaspirillum seropedicae SmR1] Length = 350 Score = 379 bits (972), Expect = e-106, Method: Compositional matrix adjust. Identities = 231/350 (66%), Positives = 281/350 (80%) Query: 3 MVHTKHQDIASLDGWRAVAILIVATSHAGLGKWVPGGLGVTIFFFLSGYLITTLLLREFA 62 MVHT+H++I SLDGWRA+AI IV SHAGLG+ VPGGLGVTIFFFLSGYLITTLL+REF Sbjct: 1 MVHTQHKEIPSLDGWRALAIAIVFLSHAGLGRIVPGGLGVTIFFFLSGYLITTLLVREFD 60 Query: 63 ATGTLNIKHFYLRRLLRLTPPLLLVLVLTYVLTHLGIFKGYATWTGFFAQLFYFANYYGL 122 + ++N++HFYLRRLLRLTPPLLLVLVLTY LT LGIF G A+W+GF AQLFYFANYY L Sbjct: 61 RSDSVNVRHFYLRRLLRLTPPLLLVLVLTYTLTWLGIFTGDASWSGFLAQLFYFANYYVL 120 Query: 123 FFDAAHTVPQGTGVFWSLAVEEHFYFFFPALLLFMLRGAQLRRLPLYLGLACVLLLAWRC 182 FFD HT+PQGTGVFWSLAVEEHFYF FPAL +F++ A R++PLYLG C+L+L WR Sbjct: 121 FFDPGHTLPQGTGVFWSLAVEEHFYFVFPALFIFLMHKADRRKIPLYLGGLCLLVLLWRY 180 Query: 183 YLVMGVGVVTERTYYATDTRIDSMLYGCILATTALSPDWQRSLQRPLLRTLAILAGVILL 242 LV+ +RTYYATDTRIDS+L+GCILAT L P +L +P +R + GV+LL Sbjct: 181 GLVLLGDASMDRTYYATDTRIDSILFGCILATLKLPPAVYAALHKPAVRVGGVTLGVLLL 240 Query: 243 LATLVVRSEVFRETVRYSLQGIALIPLFYYSVCNPEIWPFRWLNQGWVRRIGIYSYSIYL 302 L +L VR EVFRET+RYS+QG+AL+PLF+YSV +P +PFR LN WV+RIGIYSYSIYL Sbjct: 241 LLSLAVRDEVFRETLRYSVQGLALMPLFFYSVAHPHSFPFRLLNLRWVQRIGIYSYSIYL 300 Query: 303 AHYVMLNNVDWVSPYAPLNVLVALAIAWLFAVLVDRFVDTPLRVVRARLR 352 +H ++L+NVDWVS A +N+ VA + LFA+ VDR +D PLR++RAR R Sbjct: 301 SHEILLSNVDWVSHNAAVNIAVAAVFSLLFAMAVDRCIDAPLRMLRARFR 350 >gi|300311522|ref|YP_003775614.1| O-antigen acetylase [Herbaspirillum seropedicae SmR1] Length = 680 Score = 91.7 bits (226), Expect = 9e-20, Method: Compositional matrix adjust. Identities = 108/380 (28%), Positives = 174/380 (45%), Gaps = 56/380 (14%) Query: 2 SMVHTKHQDIASLDGWRAVAILIVATSHAGLGKWVPGG-LGVTIFFFLSGYLITTLLLRE 60 ++ H+K++ + +DG RA+A+L V + H G +V GG +GV +FF +SGYLI+T++ E Sbjct: 8 ALSHSKYR--SDIDGLRAIAVLAVVSFH-GFPNFVRGGFIGVDVFFVISGYLISTIIF-E 63 Query: 61 FAATGTLNIKHFYLRRLLRLTPPLLLVLVLTYVL--------THLGIFKGYATWTGFFAQ 112 GT FY RR+ R+ P LLLVL +V+ + + K A GF + Sbjct: 64 NLDRGTFTFTEFYARRVRRIFPALLLVLSTCFVVGWFVLLTDEYKQLGKHIAGGAGFVSN 123 Query: 113 LFYFANYYGLFFDAAHTVPQGTGVFWSLAVEEHFYFFFPALLLFMLRGAQLRRLPLYLGL 172 F + G F ++A T P WSL +EE FY +P LL F R R+ L L + Sbjct: 124 -FLLRDESGYFDNSAETKPLLH--LWSLGIEEQFYILWPMLLFFSWR----RKFNL-LTI 175 Query: 173 ACVLLLAWRCYLVMGVGVVTERTYYATDTRIDSMLYGCILATTALSPDWQRSLQRPLLRT 232 ++ LA + G+ +Y +R +L G +LA L RP L T Sbjct: 176 TIIVALASFAANIKGINKDATAAFYLPQSRFWELLSGTLLAWFTLYKQHTTIELRPRLGT 235 Query: 233 LAILAGVILLLATLVVRSEVFRETVRYSLQGIALIPLFYYSVCN----PEIW-------- 280 ++ L+ + +S+ S+ G+ ++ ++ + P +W Sbjct: 236 W-----ILQLIYSEKRKSDGKFLIDVSSITGLCIVLYGFWRINKELAFPGVWALVPILGT 290 Query: 281 -------PFRWLNQGWVRR-----IGIYSYSIYLAHYVMLNNVDWVS---PYAPLNV-LV 324 P W+N+ + G+ S+ +YL H+ +L+ V P PL V + Sbjct: 291 ILIIAAGPAAWVNRTLLSNKILVWFGLISFPLYLWHWPVLSFARIVENELPSYPLRVGAI 350 Query: 325 ALAI--AWLFAVLVDRFVDT 342 ALAI AWL +V+R + T Sbjct: 351 ALAIILAWLTYEIVERPIRT 370 >gi|300310014|ref|YP_003774106.1| o-antigen acetylase [Herbaspirillum seropedicae SmR1] Length = 681 Score = 80.9 bits (198), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 105/353 (29%), Positives = 169/353 (47%), Gaps = 35/353 (9%) Query: 13 SLDGWRAVAILIVATSHAGLGKWVPGG-LGVTIFFFLSGYLITTLLLREFAATGTLNIKH 71 +DG RA+AI+ V HA +PGG +GV IFF +SG+LI++++ R+ G + Sbjct: 35 DIDGLRAIAIVSVVVFHA-FPDAMPGGFIGVDIFFVISGFLISSIIFRQL-DQGRFSFTD 92 Query: 72 FYLRRLLRLTPPLLLVLVLTYVL--------THLGIFKGYATWTGFFAQLFYFANYYGLF 123 FY+ R+ R+ P L+LVL +VL + + K A GF L + + Sbjct: 93 FYVHRIRRIFPALILVLASCFVLGWFSLLPEEYRQLGKHMAAGAGFIQNLVLWKE--SGY 150 Query: 124 FDAAHTVPQGTGVFWSLAVEEHFYFFFPALLLFMLRGAQLRRLPLYLGLACVLLLAWRCY 183 FD + + WSL+VEE FY +P L+ + R R+PL L ++L ++ Sbjct: 151 FDVESDM-KPLLHLWSLSVEEQFYLVYPLLVCGLWRA----RMPLRNSLVLLMLASFALN 205 Query: 184 LVMGVGVVTERTYYATDTRIDSMLYGCILATTALSPD---WQRSLQRPLLRTLAILAGVI 240 L V +T+Y TR+ +L G + A + S + P L ++ L V+ Sbjct: 206 L-WWVAQDPVKTFYLPFTRVWELLAGALCAFGVMRKGVAFSTSSFRLPHLPSIH-LRSVL 263 Query: 241 LLLATLVVRSEVFRETVRYSLQGIALIPLFYYSVCNPEIWPFRWLNQGWVRR-----IGI 295 L L + R RY AL+P+ +V P W+N+ + R IG+ Sbjct: 264 GLALLLCGLVLIDRH--RYFPGAWALLPV-TGAVLLVSSGPQAWVNRHLLSRPLLVWIGL 320 Query: 296 YSYSIYLAHYVMLNNVDWVSPY---APLNVLVALAIAWLFAVLVDRFVDTPLR 345 SY +YL H+ +L+ + ++P APL L A+A++ L A L R ++ P+R Sbjct: 321 VSYLLYLWHWPILSFMHILTPTRPGAPLP-LAAVALSVLLAWLTYRLLERPVR 372 >gi|300311314|ref|YP_003775406.1| O-antigen acetylase [Herbaspirillum seropedicae SmR1] Length = 662 Score = 73.9 bits (180), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 57/155 (36%), Positives = 79/155 (50%), Gaps = 12/155 (7%) Query: 13 SLDGWRAVAILIVATSHAGLGKWVPGG-LGVTIFFFLSGYLITTLLLREFAATGTLNIKH 71 +DG RAVA+ V HA +W GG +GV IFF +SG+LI+ +L + G +I Sbjct: 22 DIDGLRAVAVGSVVIFHA-FPEWFAGGFIGVDIFFVISGFLISLILFKNL-EHGRFSIVD 79 Query: 72 FYLRRLLRLTPPLLLVLVLTYVLTHLGIFKGYATWTG-------FFAQLFYFANYYGLFF 124 FY+RR+ R+ P L+ V+ L V +F G F F F G F Sbjct: 80 FYVRRVRRIFPALMTVMSLCLVTGWFVLFADEYKQLGKHLFGGSTFISNFLFWRESGYFD 139 Query: 125 DAAHTVPQGTGVFWSLAVEEHFYFFFPALLLFMLR 159 ++A T P WSLA+EE FY F+P LL F+ + Sbjct: 140 NSAETKPLLH--LWSLAIEEQFYLFWPLLLAFVWK 172 >gi|300310314|ref|YP_003774406.1| acyltransferase [Herbaspirillum seropedicae SmR1] Length = 656 Score = 64.3 bits (155), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 57/153 (37%), Positives = 79/153 (51%), Gaps = 18/153 (11%) Query: 13 SLDGWRAVAILIVATSHAGLGKWVPGG-LGVTIFFFLSGYLITTLLLREFAATGTLNIKH 71 +DG RA+A+L V HA +W+PGG +GV IFF +SG+LI+ +L +I Sbjct: 25 DIDGLRAIAVLSVVVFHA-FPEWLPGGFVGVDIFFVISGFLISGILFDALQRD-RFSIVD 82 Query: 72 FYLRRLLRLTPPLLLV----------LVLTYVLTHLGIFKGYATWTGFFAQLFYFANYYG 121 FY+RR+ R+ P LLLV ++L LG K GF + + + N G Sbjct: 83 FYIRRIRRIFPALLLVMLACLLAGWFILLPEEYRQLG--KHLLGGAGFVSNIVLW-NEAG 139 Query: 122 LFFDAAHTVPQGTGVFWSLAVEEHFYFFFPALL 154 F +A T P WSL VEE FY F+P +L Sbjct: 140 YFDTSAETKPLLH--LWSLGVEEQFYLFWPLML 170 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.333 0.145 0.472 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 968,441 Number of extensions: 35511 Number of successful extensions: 174 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 162 Number of HSP's successfully gapped: 6 Length of query: 352 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 266 Effective length of database: 1,212,660 Effective search space: 322567560 Effective search space used: 322567560 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (22.0 bits) S2: 131 (55.1 bits)