BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1833.p (750 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311292|ref|YP_003775384.1| (p)ppGpp synthetase I [Herbaspi... 1474 0.0 gi|300311444|ref|YP_003775536.1| bifunctional (P)ppGpp synthetas... 360 e-100 >gi|300311292|ref|YP_003775384.1| (p)ppGpp synthetase I [Herbaspirillum seropedicae SmR1] Length = 749 Score = 1474 bits (3816), Expect = 0.0, Method: Compositional matrix adjust. Identities = 723/750 (96%), Positives = 736/750 (98%), Gaps = 1/750 (0%) Query: 1 MVSVASTQGEGLAAITAGLGPDDAARVEQALAELEPLYADRQILSGQNALQFAVSVATVL 60 MVSVASTQGEGLAA+TAGLGP+DAARV QALAELEPLYADRQILSGQNALQF++SVATVL Sbjct: 1 MVSVASTQGEGLAALTAGLGPEDAARVAQALAELEPLYADRQILSGQNALQFSISVATVL 60 Query: 61 TALNVDADTRIAGLAFEVQMLYPELDEKLEERFGKEIADLVGNVRQLMRFHSLSFQPQNQ 120 TALNVDA TRIA LAFE QML+PEL+EKLEERFGKEIADLVGNVRQLMRFHS SFQPQ Q Sbjct: 61 TALNVDAATRIAALAFEAQMLHPELEEKLEERFGKEIADLVGNVRQLMRFHSFSFQPQ-Q 119 Query: 121 EVLRGKNAAQQAAAQVETLRKMLLAMATDMRAVLVRLASRVATLRYFAETKQENEASAQY 180 EVLRGKNAAQQAAAQ+ETLRKMLLAMATDMRAVLVRLASRVATLRYFAETKQE+EASA Y Sbjct: 120 EVLRGKNAAQQAAAQIETLRKMLLAMATDMRAVLVRLASRVATLRYFAETKQEDEASANY 179 Query: 181 ARQTFELYAPLANRLGIWQLKWELEDLSFRFLEPVTYKRIAKMLEEKRLEREAFVANAIA 240 ARQTFELYAPLANRLGIWQLKWELEDLSFRFLEPVTYKRIAKMLEEKRLEREAFVANAIA Sbjct: 180 ARQTFELYAPLANRLGIWQLKWELEDLSFRFLEPVTYKRIAKMLEEKRLEREAFVANAIA 239 Query: 241 RLRAEMAAQEIRAEVSGRPKHIYSIWNKMRGKSIDFSELYDVRAFRVIVDDVKTCYAVLG 300 RLRAEMAAQ+IRAEVSGRPKHIYSIWNKMRGK IDFSELYDVRAFRVIVDDVKTCYAVLG Sbjct: 240 RLRAEMAAQDIRAEVSGRPKHIYSIWNKMRGKHIDFSELYDVRAFRVIVDDVKTCYAVLG 299 Query: 301 IVHNIWTPIPEEFDDYISRPKQNGYQSLHTVVMAEDGRALEVQIRTHEMHHFAEYGVAAH 360 IVH+IWTPIPEEFDDYISRPKQNGYQSLHTVV AEDGRALEVQIRTHEMHHFAEYGVAAH Sbjct: 300 IVHDIWTPIPEEFDDYISRPKQNGYQSLHTVVTAEDGRALEVQIRTHEMHHFAEYGVAAH 359 Query: 361 WRYKESGGSNFSAQKYDEKIAWLRQLLAWKNEVTDAVVDQEEVRRDWVQKLKSATLDERI 420 WRYKESGGSNFSAQKYDEKIAWLRQLLAWKNEVTDAVVDQEEVRRDWV+KLKSATLDERI Sbjct: 360 WRYKESGGSNFSAQKYDEKIAWLRQLLAWKNEVTDAVVDQEEVRRDWVEKLKSATLDERI 419 Query: 421 YVLTPQARVIELPSGATPIDFAYHLHSDVGHRCRGARVDGVMVPLNTTLKNGQTVEIITA 480 YVLTPQARVIELPSGATPIDFAYHLHSDVGHRCRGARVDGVMVPLNT LKNGQTVEIIT Sbjct: 420 YVLTPQARVIELPSGATPIDFAYHLHSDVGHRCRGARVDGVMVPLNTALKNGQTVEIITV 479 Query: 481 KSGPGVGPSRDWLSPDYAASSRTRSKIRAWFNAIEQQETLSVGRGLLEKTLQREGKTAVN 540 KSGPGVGPSRDWLSPDYA SSRTRSKIRAWFNAIEQQETLSVGRGLLEKTLQREGKTAVN Sbjct: 480 KSGPGVGPSRDWLSPDYAVSSRTRSKIRAWFNAIEQQETLSVGRGLLEKTLQREGKTAVN 539 Query: 541 LEELAQKLGFAKVDDLCLSLAKEEFSLRHVEQALHEGQEKKPEIDDEALITRKSRASSIV 600 LEELAQKLGFAKVDDLCLSLAKEEFSLRHVEQALHEGQEKKPEIDDE LITRKSRASSIV Sbjct: 540 LEELAQKLGFAKVDDLCLSLAKEEFSLRHVEQALHEGQEKKPEIDDETLITRKSRASSIV 599 Query: 601 QGAKSGVLVVGTDGLLTQLARCCKPAPPDVIAGFVTRGKGVSIHRVNCKNFAEMSSHAPE 660 QGAKSGVLVVGTDGLLTQLARCCKPAPPD IAGFVTRGKGVSIHRV+CKNFAEMSSHAPE Sbjct: 600 QGAKSGVLVVGTDGLLTQLARCCKPAPPDAIAGFVTRGKGVSIHRVSCKNFAEMSSHAPE 659 Query: 661 RVIQTTWGAPARDTVYPVDIFVLASDRQGLLRDISDIFLRDKINVIGVSTQSVKGHARMA 720 RVIQTTWGAPAR+TVYPVDIFVLASDRQGLLRDISDIFLRDKINVIGVSTQSVKGHARMA Sbjct: 660 RVIQTTWGAPARETVYPVDIFVLASDRQGLLRDISDIFLRDKINVIGVSTQSVKGHARMA 719 Query: 721 FTAEIGSTEQLQKALTVIREVKGVIEAKRQ 750 FTAEIGSTEQLQKALTVIREVKGVIEAKRQ Sbjct: 720 FTAEIGSTEQLQKALTVIREVKGVIEAKRQ 749 >gi|300311444|ref|YP_003775536.1| bifunctional (P)ppGpp synthetase II/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 753 Score = 360 bits (923), Expect = e-100, Method: Compositional matrix adjust. Identities = 230/743 (30%), Positives = 367/743 (49%), Gaps = 55/743 (7%) Query: 19 LGPDDAARVEQALAELEPLYADRQILSGQNALQFAVSVATVLTALNVDADTRIAGLAFEV 78 L P + +V++A + ++ + SG+ + ++VA + +DA +A +V Sbjct: 53 LTPSELKKVKEAYRFADEMHLGQMRKSGEPYISHPLAVAEICAEWKLDAQAMMAAFLHDV 112 Query: 79 QMLYPELDEKLEERFGKEIADLVGNVRQLMRFHSLSFQPQNQEVLRGKNAAQQAAAQVET 138 E+L ERFG +A LV + +L + + FQ Q AQ E Sbjct: 113 MEDQGVKKEELIERFGASVASLVDGLSKLDK---IEFQ-------------SQVEAQAEN 156 Query: 139 LRKMLLAMATDMRAVLVRLASRVATLRYFAETKQENEASAQYARQTFELYAPLANRLGIW 198 RKMLLAMA D+R +LV+LA R+ +R E + + +R+T E+Y P+A+RLG+ Sbjct: 157 FRKMLLAMARDVRVILVKLADRLHNMRTLGSMPPEKKR--RISRETMEVYVPIAHRLGLN 214 Query: 199 QLKWELEDLSFRFLEPVTYKRIAKMLEEKRLEREAFVANAIARLRAEMAAQEIRAEVSGR 258 + EL++LSF L P+ ++ ++K ++ R R V + + + + A I A+V GR Sbjct: 215 NIYRELQELSFAHLHPLRHRTLSKAVKAARGNRREVVTKIMESVTSTLIAAGIPAQVDGR 274 Query: 259 PKHIYSIWNKMRGKSIDFSELYDVRAFRVIVDDVKTCYAVLGIVHNIWTPIPEEFDDYIS 318 K +Y I+ KMR K + FS++ DV FRV+VD CY LG +H+++ P+P +F DYI+ Sbjct: 275 EKTLYGIYRKMRNKHLSFSQVLDVYGFRVVVDSFANCYVALGTLHSLFKPMPGKFKDYIA 334 Query: 319 RPKQNGYQSLHTVVMAEDGRALEVQIRTHEMHHFAEYGVAAHWRYKESGGSNFSAQKYDE 378 PK NGYQSLHT ++ G +E QIRT EMH AE GVAAHW YK+ GS Q+ Sbjct: 335 IPKLNGYQSLHTTLIGPYGTPVEFQIRTSEMHRVAESGVAAHWLYKDDEGSLTDLQQRTH 394 Query: 379 KIAWLRQLLAWKNEVTDAVVDQEEVRRDWVQKLKSATLDERIYVLTPQARVIELPSGATP 438 AWL+ LL + + D+ ++++ +K + +YV TP++++I LP GAT Sbjct: 395 --AWLQSLLDIQKQTGDSA--------EFLEHVKVDLFPDSVYVFTPKSKIIALPRGATA 444 Query: 439 IDFAYHLHSDVGHRCRGARVDGVMVPLNTTLKNGQTVEIITAKSGPGVGPSRDWLSPDYA 498 +DFAY +H+D+G + A ++ PL L NG VEIIT+ P PS +WL Y Sbjct: 445 LDFAYSIHTDIGDQTTSAIINHEPAPLRAELHNGDIVEIITS---PTSRPSPNWLG--YV 499 Query: 499 ASSRTRSKIRAWFNAIEQQETLSVGRGLLEKTLQREG----KTAVNLEELAQKLGFAKVD 554 + + RS IR Q E+ +VGR LL L G + +E L + +D Sbjct: 500 RTGKARSAIRHRLRTANQVESQAVGRRLLGNALHTLGIEPELPSHIVERLLNESSAKTMD 559 Query: 555 DLCLSLA----KEEFSLRHVEQALHEGQEKKPEIDDEALITRKSRASSIVQGAKSGVLVV 610 DL + RH+ + G P++D E ++ V++ Sbjct: 560 DLYAEIGIGTRMAPLVARHIMTMMDTGV-SVPQLDPEG---------HMLPNKPDPVVIT 609 Query: 611 GTDGLLTQLARCCKPAPPDVIAGFVTRGKGVSIHRVNCKNFAEMSSHAPERVIQTTWGAP 670 G++G QL+ CC P P D + G++ + + +H C + P+R I+ WG Sbjct: 610 GSEGASAQLSSCCMPIPGDRLTGYLKADQTLIVHTQECDTAKRLHEKEPDRWIELVWGQD 669 Query: 671 ARDTVYPVDIFVLASDRQGLLRDISDIFLRDKINVIGVSTQSVKGHARMA---FTAEIGS 727 + + I +L + +G L I+ N++ V+ G+A M FT ++ Sbjct: 670 L-NRRFDCRITILIHNERGTLARIAAEIGESDANIVSVAMDDEGGNASMKYLRFTIQVED 728 Query: 728 TEQLQKALTVIREVKGVIEAKRQ 750 L + + IR + V R+ Sbjct: 729 RVHLARTMRGIRRIDSVARILRE 751 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,234,473 Number of extensions: 92782 Number of successful extensions: 309 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 304 Number of HSP's successfully gapped: 2 Length of query: 750 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 658 Effective length of database: 1,184,250 Effective search space: 779236500 Effective search space used: 779236500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)