BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1825.p (167 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311287|ref|YP_003775379.1| 6,7-dimethyl-8-ribityllumazine ... 288 2e-79 gi|300312880|ref|YP_003776972.1| 6,7-dimethyl-8-ribityllumazine ... 59 3e-10 >gi|300311287|ref|YP_003775379.1| 6,7-dimethyl-8-ribityllumazine synthase [Herbaspirillum seropedicae SmR1] Length = 169 Score = 288 bits (737), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 152/169 (89%), Positives = 160/169 (94%), Gaps = 2/169 (1%) Query: 1 MSQTHLSAVPSHHAQRIAFVQASWHKDIVDQCRIGFTEEMAKLGWPASSIDFFELPGAFE 60 MSQ+HLSAV SHH QRIAF+QA WHKDIVDQCRI FTEEMAKLGWPA+SIDFFELPGAFE Sbjct: 1 MSQSHLSAVSSHHGQRIAFIQACWHKDIVDQCRIAFTEEMAKLGWPAASIDFFELPGAFE 60 Query: 61 IPLHAKVLAKTGKYAGIVASGLVVDGGIYRHDFVAQSVISALMQVQLETEVPVFSAVLTP 120 IPLHAKVLAKTG+YAGIVASGLVVDGGIYRHDFVAQSVISALMQVQL+TEVPVFSAVLTP Sbjct: 61 IPLHAKVLAKTGRYAGIVASGLVVDGGIYRHDFVAQSVISALMQVQLDTEVPVFSAVLTP 120 Query: 121 HHFHAHSEHHDYFHAHFLVKGREAAQACASTVAKLAEVRAA--RSLNAA 167 HHFH+HSEHHDYFH HFLVKGREAAQACA+TVAKLA+VRA SLNAA Sbjct: 121 HHFHSHSEHHDYFHQHFLVKGREAAQACATTVAKLAQVRAGAQASLNAA 169 >gi|300312880|ref|YP_003776972.1| 6,7-dimethyl-8-ribityllumazine synthase [Herbaspirillum seropedicae SmR1] Length = 161 Score = 58.5 bits (140), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 41/141 (29%), Positives = 70/141 (49%), Gaps = 8/141 (5%) Query: 16 RIAFVQASWHKDIVDQCRIGFTEEMAKLGWPASSIDFFELPGAFEIPLHAKVLAKTGKYA 75 RI VQA +++D+ E+ LG I +PGA EIPL + +A+T ++ Sbjct: 16 RIGIVQARFNEDVCHGLLAACLAELKHLGVADEDILHVTVPGALEIPLALQKMAETEQFD 75 Query: 76 GIVASGLVVDGGIYRHDFVAQSVISALMQVQLETEVPVFSAVLTPHHFHAHSEHHDYFHA 135 ++A G V+ G Y + V+ + + ++ L+ VP+ +AVLT +E+ + Sbjct: 76 ALIALGAVIRGETYHFELVSNESGAGITRIGLDFGVPIANAVLT-------TENDEQAEV 128 Query: 136 HFLVKGREAAQACASTVAKLA 156 KG +AA+ A +A LA Sbjct: 129 RMAEKGADAAR-VAVEMANLA 148 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.132 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 499,233 Number of extensions: 19373 Number of successful extensions: 100 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 100 Number of HSP's successfully gapped: 2 Length of query: 167 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 88 Effective length of database: 1,245,805 Effective search space: 109630840 Effective search space used: 109630840 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 127 (53.5 bits)