BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1760.p (685 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311219|ref|YP_003775311.1| glutamine-dependent NAD synthet... 1352 0.0 gi|300313858|ref|YP_003777950.1| NH(3)-dependent NAD(+) syntheta... 121 2e-28 >gi|300311219|ref|YP_003775311.1| glutamine-dependent NAD synthetase [Herbaspirillum seropedicae SmR1] Length = 685 Score = 1352 bits (3498), Expect = 0.0, Method: Compositional matrix adjust. Identities = 650/685 (94%), Positives = 670/685 (97%) Query: 1 MPSPFFNLYHHGFARVAVAVPECKVADPGFNAQRTIALAEQAAARGAALVSFPELGLSAY 60 M SPFFNLY HGFARVAVAVP+CKVADPGFNAQRTIALAEQAAARGA LVSFPELGLSAY Sbjct: 1 MQSPFFNLYSHGFARVAVAVPDCKVADPGFNAQRTIALAEQAAARGAVLVSFPELGLSAY 60 Query: 61 SCEDLFQQRALLDASLQALQAVLEASLRIPAALIVGLPLKVDHQLYNCAVVLHCGRILGV 120 SCEDLFQQRALLDASLQALQ+VLEAS+RIPAALIVGLPLKVDHQLYNCAVVLH GRILGV Sbjct: 61 SCEDLFQQRALLDASLQALQSVLEASMRIPAALIVGLPLKVDHQLYNCAVVLHHGRILGV 120 Query: 121 VPKSYLPNYSEFYEARQFSSADCAVTRRVRLLGQEVGFGSHLLFEIANIADFRFHIEICE 180 VPKSYLPNYSEFYEARQFSSADCAVTR V LLGQEVGFGSHLLFEI NI DFRFHIEICE Sbjct: 121 VPKSYLPNYSEFYEARQFSSADCAVTRSVHLLGQEVGFGSHLLFEIRNIPDFRFHIEICE 180 Query: 181 DVWVPIPPSSFAALAGATVLVNLSASNIVVGKSGYRHQLVSQQSARCLAAYLYSSAGNGE 240 DVWVPIPPSSFAALAGATVLVNLSASNIVVGKSGYRHQLVSQQSARCLAAYLYSSAGNGE Sbjct: 181 DVWVPIPPSSFAALAGATVLVNLSASNIVVGKSGYRHQLVSQQSARCLAAYLYSSAGNGE 240 Query: 241 STTDLAWDGQALICENGELLAESERFAEGGHVIYADIDLERLSRERFHQTTFGQSVRRHA 300 STTDLAWDGQALICENGELLAESERFA+GGHVIYAD+DLERLSRERFHQTTFGQSVRRHA Sbjct: 241 STTDLAWDGQALICENGELLAESERFAQGGHVIYADVDLERLSRERFHQTTFGQSVRRHA 300 Query: 301 EEVRRFEVVAFEVQVPQEQVLPLQRRVARFPYVPANAEQRELRCREVYNIQVQALVQRLS 360 +EVRRFEVV FEVQ+PQ+Q LPLQRRVARFPYVPANAEQRELRCREVY+IQVQALVQRLS Sbjct: 301 DEVRRFEVVGFEVQIPQQQALPLQRRVARFPYVPANAEQRELRCREVYSIQVQALVQRLS 360 Query: 361 SSGLKKVVIGVSGGLDSTHALLVCAKAMDKLGLPRTNILAYTMPGFATSSRTLVQAHQLM 420 S+GLKKVVIGVSGGLDSTHALLVCAKAMDKLGLPR+NILAYTMPGFATSSRTLVQAHQLM Sbjct: 361 SAGLKKVVIGVSGGLDSTHALLVCAKAMDKLGLPRSNILAYTMPGFATSSRTLVQAHQLM 420 Query: 421 AQVGCAAQEIDIRPSCEQMLRDLGHPYSRGEPVYDITFENVQAGERTNHLFRLANHHGAI 480 AQVGC+AQEIDIRPSCEQML+DLGHPYSRGEPVYDITFENVQAGERTNHLFRLANHHGAI Sbjct: 421 AQVGCSAQEIDIRPSCEQMLKDLGHPYSRGEPVYDITFENVQAGERTNHLFRLANHHGAI 480 Query: 481 VIGTGDLSELALGWCTYGVGDHMSHYNVNASVPKTLITHLVRWVAESGSLELKQPEVLIH 540 VIGTGDLSELALGWCTYGVGDHMSHYNVNASVPKTLITH+VRWVAESGSLELKQPEVLIH Sbjct: 481 VIGTGDLSELALGWCTYGVGDHMSHYNVNASVPKTLITHMVRWVAESGSLELKQPEVLIH 540 Query: 541 ILETEISPELVPGASEGEPGQRTQDFIGPYELQDFNLYYILRYGFTPAKVAFLAHSAWQD 600 +LETEISPELVPGAS+GEPGQRTQDFIGPYELQDFNLYYILRYGFTPAKVAFLAHSAW D Sbjct: 541 VLETEISPELVPGASDGEPGQRTQDFIGPYELQDFNLYYILRYGFTPAKVAFLAHSAWHD 600 Query: 601 RNVGHWPEGLHGARNQYSLPQIKKNLGIFVYRFFKTSQFKRSCVPNAPKVGTGGSLSPRG 660 R VGHWP+GLHGA N+YSLPQIKKNLGIFVYRFFKTSQFKRSCVPNAPKVG+GGSLSPRG Sbjct: 601 RGVGHWPDGLHGAHNEYSLPQIKKNLGIFVYRFFKTSQFKRSCVPNAPKVGSGGSLSPRG 660 Query: 661 DWRAPSDGEATVWLRDLERIPDQEA 685 DWRAPSDGEATVWL+DLERIPD E Sbjct: 661 DWRAPSDGEATVWLQDLERIPDLEG 685 >gi|300313858|ref|YP_003777950.1| NH(3)-dependent NAD(+) synthetase [Herbaspirillum seropedicae SmR1] Length = 565 Score = 121 bits (304), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 151/593 (25%), Positives = 238/593 (40%), Gaps = 106/593 (17%) Query: 15 RVAVAVPECKVADPGFNAQRTIALAEQAAARGAALVSFPELGLSAYSCEDLFQQRALLDA 74 R+A+A + D N + ++AA GA LV FPEL + Y DL ++ LD Sbjct: 3 RIAIAQCNPTIGDFEGNLALHLDGLQRAAQAGADLVVFPELSICGYYPGDLLEEARFLDR 62 Query: 75 SLQALQAVLEASLRIPA-ALIVGLPLK---VDHQLYNCAVVLHCGRILGVVPKSYLPNYS 130 + LQ V++A+ R+P ++G P + L+N + + GRI+ K LP Y Sbjct: 63 AEATLQDVIDATRRLPGLTAVIGAPRRNPGPGKPLFNALLAVREGRIVAEYHKQLLPVYG 122 Query: 131 EFYEARQFSSAD---CAVTRRVRLLGQEVGFGSHLLFEIANIADFRFHIEICEDVW---- 183 F + R F + C + L G VGF ICED W Sbjct: 123 VFDDGRHFEAGPETACVLP----LKGMRVGF------------------LICEDGWNDDL 160 Query: 184 --VPIPPSSFAALAGATVLVNLSASNIVVGKSGYRHQLVSQQSARCLAAYLYSSAGNGES 241 + P A A A ++V+++AS VGK RH L R A LY + G+ Sbjct: 161 RSYRVNPFEKLAQADAQLVVSINASPSDVGKRSQRHALFQAACLRRQMALLYVNQVGGQD 220 Query: 242 TTDLAWDGQALICENGELLA-ESERFAEGGHVIYADIDLERLSRERFHQTTFGQSVRRHA 300 L +DG + + LA E+ R AE V+ F F +S A Sbjct: 221 --QLVYDGGSFAMSPLQGLAFEAARLAEDFQVLA------------FEGGRFLRSDGVGA 266 Query: 301 EEVRRFEVVAFEVQVPQEQVLPLQRRVARFPYVPANAEQRELRCREVYNIQVQALVQRLS 360 + + E + Q+L R AR RC Sbjct: 267 PQCIDQTMATPEFH--RRQILLGLRDYAR-------------RC---------------- 295 Query: 361 SSGLKKVVIGVSGGLDSTHALLVCAKAMDKLGLPRTNILAYTMPGFATSSRTLVQAHQLM 420 G KVV+G SGG+DS + + A+A+ N++A TMP +S+ ++ + L Sbjct: 296 --GFGKVVVGSSGGIDSAVTIALAAEALGA-----QNVVAVTMPSVFSSAGSVSDSETLC 348 Query: 421 AQVGCAAQEIDIRPSCEQMLRDL--GHPYSRGEPVYDITFENVQAGERTNHLFRLANHHG 478 +G R ++R+ G + G P+ + EN+QA R L +N + Sbjct: 349 QALGVTL----FRHPIADLVREFEDGFATAFGAPLRGLPLENLQARVRGAILMEYSNGYD 404 Query: 479 AIVIGTGDLSELALGWCTYGVGDHMSHYNVNASVPKTLITHLVRWVAESGSLELKQPEVL 538 A+++ TG+ SE+++G+CT GD + + KT + L R + E+ ++ Sbjct: 405 ALLLTTGNKSEISVGYCTL-YGDTNGGLGLIGDLYKTEVYALARHLNAQAGREIIPAAII 463 Query: 539 IHILETEISPELVPGASEGEPGQRTQDFIGPYELQDFNLYYILRYGFTPAKVA 591 E++P+ QR D + PYE+ D L + + PA A Sbjct: 464 DKAPSAELAPD-----------QRDTDSLPPYEVLDEILKWHIEGSRLPADEA 505 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,059,614 Number of extensions: 83884 Number of successful extensions: 216 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 212 Number of HSP's successfully gapped: 3 Length of query: 685 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 593 Effective length of database: 1,184,250 Effective search space: 702260250 Effective search space used: 702260250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)