BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0001.1741.p
         (219 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311201|ref|YP_003775293.1| threonine efflux protein [Herba...   375   e-105
gi|300312440|ref|YP_003776532.1| threonine efflux protein [Herba...    66   2e-12

>gi|300311201|ref|YP_003775293.1| threonine efflux protein
           [Herbaspirillum seropedicae SmR1]
          Length = 219

 Score =  375 bits (963), Expect = e-105,   Method: Compositional matrix adjust.
 Identities = 189/219 (86%), Positives = 204/219 (93%)

Query: 1   MAYQTWITFFFAACVIAISPGSGAVLSMSHGLNYGVRRTSATILGLEVALVVVLVIAGAG 60
           M YQTW  FF AACVIAISPGSGAVLSMSHGLNYGVRRTSATILGLE+ALVV+L+IAGAG
Sbjct: 1   MDYQTWFAFFAAACVIAISPGSGAVLSMSHGLNYGVRRTSATILGLEIALVVILIIAGAG 60

Query: 61  VGSLLVASEPAFNAVKIAGALYLIYLGFSQWRAPVPTEQEGEAIDAANAVARAQRGQAWW 120
           VGSLLVASE AFN +K+AGALYLIYLGFSQWRA VP+E++GEA+DAANA ARA   Q WW
Sbjct: 61  VGSLLVASETAFNVIKVAGALYLIYLGFSQWRARVPSEEQGEALDAANASARAHARQTWW 120

Query: 121 QRCITGFLTNATNPKGIVFMVAVLPQFIDHERPLGLQLLVLGLTMVAVDVVVMHGYAFAA 180
           QRC+TGFLTNATNPKGIVFMVAVLPQFIDH+RPL LQLLVL +TMVAVD+VVMHGYAFAA
Sbjct: 121 QRCMTGFLTNATNPKGIVFMVAVLPQFIDHDRPLWLQLLVLAVTMVAVDLVVMHGYAFAA 180

Query: 181 SRLQRFFRSQKALKAQNRFFGGVLMLVGAGLFFVKRTQH 219
           SRLQRFFRSQKA++AQNRFFGGVLMLVGAGLFF KRTQH
Sbjct: 181 SRLQRFFRSQKAIRAQNRFFGGVLMLVGAGLFFFKRTQH 219


>gi|300312440|ref|YP_003776532.1| threonine efflux protein
           [Herbaspirillum seropedicae SmR1]
          Length = 208

 Score = 66.2 bits (160), Expect = 2e-12,   Method: Compositional matrix adjust.
 Identities = 58/197 (29%), Positives = 88/197 (44%), Gaps = 9/197 (4%)

Query: 7   ITFFFAACVIAISPGSGAVLSMSHGLNYGVRRTSATILGLEVALVVVLVIAGAGVGSLLV 66
           I F  AA  + ++PG   +  ++ G+  G +       G    LV   ++A  G  +L+ 
Sbjct: 5   ILFLLAAMTLTLAPGPDNLYVLTRGIAQGRKAGLVAAAGFCSGLVFHTLLAVLGFAALIK 64

Query: 67  ASEPAFNAVKIAGALYLIYLGFSQWRAPVPTEQEGEAIDAANAVARAQRGQAWWQRCITG 126
           A  PA++A++ AGA YL YLG    R    +     A D   A A   R + +WQ     
Sbjct: 65  AYPPAYHALQYAGAAYLAYLGVRTLR--SASAGLALAADGQGAAAVPLR-RIYWQSV--- 118

Query: 127 FLTNATNPKGIVFMVAVLPQFIDHER-PLGLQLLVLGLTMVAVDVVVMHGYAFAASRLQR 185
            L N  NPK  +F +A LPQF+  E     LQ+LVL L  +     +    A  +  +  
Sbjct: 119 -LANMLNPKVTLFFIAFLPQFVQREAGHEALQMLVLALVFIVQAFAIFSAIALFSGAVGA 177

Query: 186 FFRSQKALKAQ-NRFFG 201
           +FR + +     NR  G
Sbjct: 178 YFRRRASASVHLNRLAG 194


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.328    0.139    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 565,919
Number of extensions: 17662
Number of successful extensions: 98
Number of sequences better than 1.0e-08: 4
Number of HSP's gapped: 96
Number of HSP's successfully gapped: 4
Length of query: 219
Length of database: 1,619,870
Length adjustment: 82
Effective length of query: 137
Effective length of database: 1,231,600
Effective search space: 168729200
Effective search space used: 168729200
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 128 (53.9 bits)