BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1719.p (532 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311185|ref|YP_003775277.1| major facilitator superfamily p... 990 0.0 gi|300310323|ref|YP_003774415.1| methylenomycin A resistance pro... 135 1e-32 gi|300309872|ref|YP_003773964.1| major facilitator superfamily p... 127 2e-30 gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 109 7e-25 gi|300312486|ref|YP_003776578.1| major facilitator superfamily p... 107 3e-24 >gi|300311185|ref|YP_003775277.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 532 Score = 990 bits (2560), Expect = 0.0, Method: Compositional matrix adjust. Identities = 508/532 (95%), Positives = 522/532 (98%) Query: 1 MALHTDAAHSSGQVLPFRESLLATLGICFVIMLVALDQTIVGTALPTIVAELKGFELYAW 60 MALHTD+AHSSGQVLPFRESLLATLGICFVIMLVALDQTIVGTALPTIVAELKGFELYAW Sbjct: 1 MALHTDSAHSSGQVLPFRESLLATLGICFVIMLVALDQTIVGTALPTIVAELKGFELYAW 60 Query: 61 VATAYLLTSVITVPIFGRLGDYFGRKPFVIASIIVFVGASALCGMANSMLFLVLARGLQG 120 VATAYLLTSVITVPIFGRLGDYFGRKPFVIASIIVFVGASALCGMANSMLFLVLARGLQG Sbjct: 61 VATAYLLTSVITVPIFGRLGDYFGRKPFVIASIIVFVGASALCGMANSMLFLVLARGLQG 120 Query: 121 IGGGMLVGTCFASIADLFPDPRVRLRWQVILSASFGIATAVGPSLGGFLTEGLGWRWVFY 180 IGGGMLVGTCFAS+ADLFPDPRVRLRWQ+ILS SFGIATAVGPSLGGFLT+GLGWRWVFY Sbjct: 121 IGGGMLVGTCFASVADLFPDPRVRLRWQIILSVSFGIATAVGPSLGGFLTQGLGWRWVFY 180 Query: 181 CNLPIGVISLFFVWRFVPHIRHMHHAGKMRLDWPGALLISLSLGSLQLLVELLPKRGITG 240 CNLPIGVISLFFVWRFVPHIRHM HAGKMRLDWPGALLISLSLGSLQLLVELLPKRG+T Sbjct: 181 CNLPIGVISLFFVWRFVPHIRHMQHAGKMRLDWPGALLISLSLGSLQLLVELLPKRGVTS 240 Query: 241 GMMALLAVSVAAFYALWKWEQRAAQAILPFEMLRDRALASLFLLSVLAGFAMFSLLMYAP 300 GM+ LLAVSVAAFYALWKWEQRAAQ ILPFEMLRDRALASLFLLSVLAGFAMFS+LMYAP Sbjct: 241 GMLVLLAVSVAAFYALWKWEQRAAQPILPFEMLRDRALASLFLLSVLAGFAMFSMLMYAP 300 Query: 301 LLFQGGFGLSPREAGLLITPLVACITVASIVNGRIITRIPNPNLMMNLGFALIAASCLGV 360 LLFQGGFGLSPREAGLLITPLVACITVASIVNGRIITRIPNPNLMMNLGFALIAASCLG+ Sbjct: 301 LLFQGGFGLSPREAGLLITPLVACITVASIVNGRIITRIPNPNLMMNLGFALIAASCLGI 360 Query: 361 VLSDRNTGNGFLLAVMLAGGFGLGLVLPNLTVFAQQAAAREHLGIATALLQSLRMIGGML 420 VL DR TG GFLLAVMLAGGFGLGLVLPNLTVFAQQAAAREHLGIATALLQSLRMIGGML Sbjct: 361 VLCDRVTGKGFLLAVMLAGGFGLGLVLPNLTVFAQQAAAREHLGIATALLQSLRMIGGML 420 Query: 421 GTAVTGTLVSQMYGAGVQKALESDHAAQWLKEFSDPEVLVNHDIQSVLLARLMQAGHDGS 480 GTA+TGTLVSQMYGAGV+KALE DHAAQWLK+FSDPEVLVNHDIQSVLLA+LMQAGHDGS Sbjct: 421 GTAITGTLVSQMYGAGVEKALEGDHAAQWLKDFSDPEVLVNHDIQSVLLAQLMQAGHDGS 480 Query: 481 ALLEAAREALVGAIHMGIAIAIVAALVGLWMVGRVPPIRFDQDQKVDPVMSE 532 ALLEAAREALVGAIHMGIAIAIVAALVGLWMV RVPPIRFDQD+KV+PVMSE Sbjct: 481 ALLEAAREALVGAIHMGIAIAIVAALVGLWMVRRVPPIRFDQDKKVEPVMSE 532 >gi|300310323|ref|YP_003774415.1| methylenomycin A resistance protein [Herbaspirillum seropedicae SmR1] Length = 478 Score = 135 bits (339), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 125/428 (29%), Positives = 203/428 (47%), Gaps = 21/428 (4%) Query: 22 LATLGICFVIMLVALDQTIVGTALPTIVAELKG-FELYAWVATAYLLTSVITVPIFGRLG 80 L L I FV+ + LD T+V L I L W+ Y LT + + G L Sbjct: 32 LVALAIGFVMAM--LDVTVVNVGLSNIAGSLDAPLSTLVWIVDGYTLTFAAMLLVGGALA 89 Query: 81 DYFGRKPFVIASIIVFVGASALCGMANSMLFLVLARGLQGIGGGMLVGTCFASIADLFPD 140 D FG + + + +FV AS LCG A + FL++AR LQG+G + + + + ++ D Sbjct: 90 DRFGARRLYLTGLFLFVLASLLCGAAPNGPFLIVARLLQGLGAAFFMPSSLSLLTHVYED 149 Query: 141 PRVRLRWQVILSASFGIATAVGPSLGGFLTEGLGWRWVFYCNLPIGVISLFFVWRFVPHI 200 RVR R + SA+ G+A AVGP +GG L LGWR VF N+P+G++ L + +P + Sbjct: 150 DRVRARMLGVWSATVGLAAAVGPLVGGVLIHWLGWRSVFLINVPVGLVGLVMARKLIPQV 209 Query: 201 RHMHHAGKMRLDWPGALLISLSLGSLQLLVELLPKRGITGG-MMALLAVSVAAFYALWKW 259 A + G ++ L L ++ P G T ++A LAV+++A AL + Sbjct: 210 GGHARALNLSSHLAGVVM----LAGLSFVLIQGPVYGWTSPRILAGLAVALSAGAALIRH 265 Query: 260 EQRAAQAILPFEMLRDRALASLFLLSVLAGFAMFSLLMYAPLLFQGGFGLSPREAGLLIT 319 E R +LP + A+ + L F ++ L Y L Q G G + GL + Sbjct: 266 ELRGIAPLLPKALFATPQFAAANGIGFLINFGVYGNLFYLALFLQQGRGADALQTGLQLV 325 Query: 320 PLVACITVASIVNGRIITRIPNPNLMMNLGFALIAASCLGVVLSDRNTGNG-----FLLA 374 P++A I ++++GR+ P + + LG + +G V + G +LLA Sbjct: 326 PMMAVIFFGNLMSGRMSAHW-GPRVPLLLGLS------VGTVFTALAMGFSPATPYWLLA 378 Query: 375 VMLA-GGFGLGLVLPNLTVFAQQAAAREHLGIATALLQSLRMIGGMLGTAVTGTLVSQMY 433 ++ A G+ +P +T Q A + H A A L + R IG ++G A+ G ++ + Sbjct: 379 LVCASANLGISTAIPAMTTVVMQVAGKAHANSAAAALNANRQIGALVGVALMGAILHSVT 438 Query: 434 GAGVQKAL 441 G ++ L Sbjct: 439 GWNLRNPL 446 >gi|300309872|ref|YP_003773964.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 493 Score = 127 bits (319), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 129/452 (28%), Positives = 203/452 (44%), Gaps = 25/452 (5%) Query: 1 MALHTDAAHSSGQVLPFRESLLATLGICFVIMLVALDQTIVGTALPTIVAELK-GFELYA 59 M L +D H + + LP ++ A L + + D TI+ ALP I L+ + Sbjct: 10 MELFSD--HPNDEGLPGKQRRRAMLAVMTATTMAVFDGTIINVALPQISQALQTSAAVSI 67 Query: 60 WVATAYLLTSVITVPIFGRLGDYFGRKPFVIASIIVFVGASALCGMANSMLFLVLARGLQ 119 WV+ AYLL + +T+ F L G + A + VF AS C ++ S+ L+L R LQ Sbjct: 68 WVSNAYLLATAMTLAAFAALSSRIGFRAQFSAGLAVFTLASLGCALSGSVTLLILMRILQ 127 Query: 120 GIGGGMLVGTCFASIADLFPDP---RVRLRWQVILSASFGIATAVGPSLGGFLTEGLGWR 176 G+GG ++ A +FP+ R+ V+++AS TAVGP LGG L LGW Sbjct: 128 GLGGAAMLSIAPAIHRTIFPNRLLGRILGLNAVLVAAS----TAVGPVLGGTLLAMLGWE 183 Query: 177 WVFYCNLPIGVISLFFVWRFVPHIRHMHHAGKMRLDWPGALLISLSLGSLQLLVELLPK- 235 W+F N+P+GV++++ W VP R A D GAL +L++G+L + + + Sbjct: 184 WIFAINVPLGVLAVWLAWNAVPDTRKPAQAA---FDLAGALWSALAMGALIMAADTCARF 240 Query: 236 ------RGITGGMMALLAVSVAAFYALWKWEQRAAQAILPFEMLRDRALASLFLLSVLAG 289 G A ++ A A + ++RAA +LP ++ + SL L+ LA Sbjct: 241 AEPGQGSGAAFTAAAYAVTALLAGLAFVRGQRRAADPLLPLDIFANPRF-SLAALTSLAS 299 Query: 290 FAMFSLLMYA-PLLFQGGFGLSPREAGLLITPLVACITVASIVNGRIITRIPNPNLMMNL 348 F + A P LFQ +G + E+ L TP I + + GR+ R L Sbjct: 300 FVGQGIAFVALPFLFQNAYGYTAFESAALFTPWPLGIVLVAPHAGRLADRHSPALLSSLG 359 Query: 349 GFALIAASCLGVVLSDRNTGNGFLLAVMLAG-GFGLGLVLPNLTVFAQQAAAREHLGIAT 407 L +L D+ ++ G GFG N + +RE G A+ Sbjct: 360 LALFALGLVLLALLPDQAQPWDIGWRALVCGMGFGFFQSPNNREMLGN--VSRERSGNAS 417 Query: 408 ALLQSLRMIGGMLGTAVTGTLVSQMYGAGVQK 439 +L +R G LG A+ G +++ A V + Sbjct: 418 GVLAIMRTFGQCLGAALLGVILAVRTAAAVAQ 449 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 109 bits (272), Expect = 7e-25, Method: Compositional matrix adjust. Identities = 111/402 (27%), Positives = 180/402 (44%), Gaps = 21/402 (5%) Query: 36 LDQTIVGTALPTIVAELKGFELY-AWVATAYLLTSVITVPIFGRLGDYFGRKPFVIASII 94 LD TI ++ TI L +V T+Y + ITVP+ G L + FG I S++ Sbjct: 47 LDTTIANVSVSTIAGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFILSMV 106 Query: 95 VFVGASALCGMANSMLFLVLARGLQGIGGGMLVGTCFASIADLFPDPRVRLRWQVILSAS 154 +F SALCGMANS+ L+ R LQG+ GG L+ + +FP + + + Sbjct: 107 MFGLFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAPAAVGLWAMTT 166 Query: 155 FGIATAVGPSLGGFLTEGLGWRWVFYCNLPIGVISLFFVWRFVPHIRHMHHAGKMRLDWP 214 +A GP LGG L + W ++F+ N+P+ +I + WR + R+ K+ +D Sbjct: 167 L-VAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYIGWRMLK--RYETKMSKLPIDRV 223 Query: 215 GALLISLSLGSLQLLV-ELLPKRGITGGMMALLAVSVAAFY-ALWKWEQRAAQAILPFEM 272 G +L+ + + +LQL++ E K + LLAV+ F+ A WE I+ + Sbjct: 224 GLVLMVVWVAALQLMLDEGKEKDWFASTEIVLLAVTAVVFFIAFLIWELTERNPIVDLRV 283 Query: 273 LRDRALASLFLLSVLAGFAMFSLLMYAPLLFQGGFGLSPREAGLL--ITPLVACITVASI 330 R R + LA A F ++ PL QG G + +G + +T ++A +T + Sbjct: 284 FRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTATWSGCISALTGILAVMTAPMV 343 Query: 331 VNGRIITRIPNPNLMMNLGFALIAASCLGVVLSDR--NTGNGFLLAV---MLAGGFGLGL 385 ++ T I L+ I + LG + R NT + + + G GL L Sbjct: 344 --AKMSTTIDARKLV------CIGVTWLGFITFLRAFNTTDMSFFQIGWPLFLQGIGLPL 395 Query: 386 VLPNLTVFAQQAAAREHLGIATALLQSLRMIGGMLGTAVTGT 427 LT A + E A L+ R + G + T++ T Sbjct: 396 FFVPLTGLALSSVNEEETASAAGLMSFCRTMAGAIATSIVNT 437 >gi|300312486|ref|YP_003776578.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 514 Score = 107 bits (267), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 120/441 (27%), Positives = 209/441 (47%), Gaps = 36/441 (8%) Query: 32 MLVALDQTIVGTALPTIVAEL--KGFELYAWVATAYLLTSVITVPIFGRLGDYFGRKPFV 89 ++ LD +IV ++LPTI E+ G E W+ATAYL+ ++ +P+ L G + + Sbjct: 27 LMATLDISIVNSSLPTIQGEIGASGTE-GTWIATAYLVAEIVIIPMSAWLERLLGLRVLL 85 Query: 90 IASIIVFVGASALCGMANSMLFLVLARGLQGIGGGMLVGTCFASIADLFPDPRVRLRWQV 149 + + +F S LCG++ ++ +++ R QG GG L+ T IA P + Q Sbjct: 86 LTAASLFTLFSVLCGLSTTLPMMIVGRVGQGFTGGALIPTAMTIIATRLPRSQ-----QP 140 Query: 150 ILSASFGIATAV-----GPSLGGFLTEGLGWRWVFYCNLPIGVISLFFVWRFVPHIRHMH 204 + +A FG ATA+ GP +GG+LTE + W + F+ NLP+G + + +P+ R Sbjct: 141 VGNAMFG-ATAILGPVLGPVMGGWLTENVSWHYAFFINLPVGAALITLLLVAIPYERPKF 199 Query: 205 HAGKMRLDWPGALLISLSLGSLQLLVELLPKRG--ITGGMMALLAVSVAAFYALWKWEQR 262 DW G ++L LG L +++E + + + L A SV F L + R Sbjct: 200 EQ-LAEADWFGIAGLALGLGGLTVVLEEGEREQWFSSPDIRWLAATSVLGFILLAIGQVR 258 Query: 263 AAQAILPFEMLRDRALASLFLLSVLAGFAMFSLLMYAPLLFQGGFGLSPREAGLLI---- 318 A ++ +LRDR S+ +++++ G A++ P G G + ++G ++ Sbjct: 259 ARHPVIQLRLLRDRQFGSVVMMAMIVGVALYGTAYVIPQFLSGIAGYNSLQSGWIVLLSG 318 Query: 319 TPLVACITVASIVNGRIITRIPNPNLMMNLGFALIAASC-LGVVLSDRNTGNGFLLAVML 377 P++ + ++ R+ + + + GF L+AAS + LS + G F+ + ++ Sbjct: 319 VPMILMMPFTP-----LLMRLVDIRIAVGTGFLLLAASAFIETDLSPLSYGGSFVASQLM 373 Query: 378 AGGFGLGLVLPNLTVFAQQAAAR----EHLGIATALLQSLRMIGGMLGTAVTGTLVSQMY 433 G+G VL L F QAA R G A+ L + R +GG L A + Q Sbjct: 374 R---GVGTVLAML--FLNQAAIRSVPPSQAGDASGLYSAARNLGGSLALAAIAVIQDQRL 428 Query: 434 GAGVQKALESDHAAQWLKEFS 454 ++ ES +A L + S Sbjct: 429 WLHSRRLEESLNANSDLVQSS 449 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.142 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,524,490 Number of extensions: 59808 Number of successful extensions: 431 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 407 Number of HSP's successfully gapped: 16 Length of query: 532 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 442 Effective length of database: 1,193,720 Effective search space: 527624240 Effective search space used: 527624240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)