BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1718.p (454 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311183|ref|YP_003775275.1| Zn-dependent protease [Herbaspi... 913 0.0 gi|300312124|ref|YP_003776216.1| Zn-dependent protease TLDE prot... 328 6e-91 gi|300310755|ref|YP_003774847.1| Zn-dependent protease [Herbaspi... 64 2e-11 gi|300312123|ref|YP_003776215.1| Zn-dependent protease TldD prot... 60 4e-10 >gi|300311183|ref|YP_003775275.1| Zn-dependent protease [Herbaspirillum seropedicae SmR1] Length = 454 Score = 913 bits (2360), Expect = 0.0, Method: Compositional matrix adjust. Identities = 441/454 (97%), Positives = 452/454 (99%) Query: 1 MSETPFTHSQDQLKQLAQDVLRYARDKGASDAAVDISEGSGLSVAVRKGKIETIEQNKDK 60 MS+TPFTHSQDQLKQLAQDVLRYARDKGASDAAVDISEGSGLSVAVRKGKIETIEQNKDK Sbjct: 1 MSDTPFTHSQDQLKQLAQDVLRYARDKGASDAAVDISEGSGLSVAVRKGKIETIEQNKDK 60 Query: 61 GIGVTVFLGEGRQVRRGNASTSDFSQQALKETVEAAYNIARFTSIDDCAGLPEAEMLEMA 120 GIGVTVFLGEGRQVRRGNASTSDFSQQALKETVEAAYNIARFTSIDDCAGLP+AEMLEMA Sbjct: 61 GIGVTVFLGEGRQVRRGNASTSDFSQQALKETVEAAYNIARFTSIDDCAGLPDAEMLEMA 120 Query: 121 PRDLKLFSPWNISAEEAVALAQRCETAALETDARISNSEGAGVYVQHSHFIAANSRGFMG 180 PRDLKLFSPWNISAEEAVALAQRCETAALETD RISNSEGAGVYVQHSHFIAANSRGFMG Sbjct: 121 PRDLKLFSPWNISAEEAVALAQRCETAALETDPRISNSEGAGVYVQHSHFIAANSRGFMG 180 Query: 181 GYPFSRHTISVAPIAGKGAAMQRDDWYSSKRDPKKLARPEAIGRYAAERALARLNARKLS 240 GYPFSRHTISVAPIAGKGA+MQRDDWYSSKRDPKKLA+PEAIGRYAAERALARLNARKLS Sbjct: 181 GYPFSRHTISVAPIAGKGASMQRDDWYSSKRDPKKLAQPEAIGRYAAERALARLNARKLS 240 Query: 241 TRKCPVLFEAPLAAGLLGAFVQAVSGGALYRKSTFLLDSLGQQVFPEHIQILEDPHVIGG 300 TRKCPVLFEAPLAAGLLGAFVQAVSGGALYRKSTFLLDSLGQQVFPEHIQILEDPHVIGG Sbjct: 241 TRKCPVLFEAPLAAGLLGAFVQAVSGGALYRKSTFLLDSLGQQVFPEHIQILEDPHVIGG 300 Query: 301 VGSAPFDEEGVRTVKREVVKDGVVQGYFLSTYSARKLGMQTTGNSGGSHNLTLTSELTTR 360 VGSAPFDEEGVRT+KREVV++GVVQGYFLSTYSARKLGMQTTGNSGGSHNLTL SELTTR Sbjct: 301 VGSAPFDEEGVRTLKREVVRNGVVQGYFLSTYSARKLGMQTTGNSGGSHNLTLRSELTTR 360 Query: 361 ADTFKGMLKKMGTGLLVTELMGQGVNYVTGDYSRGASGYWVDNGVIQYPVEEITIAGNMK 420 ADTFKGMLKKMGTGLLVTELMGQGVNYVTGDYSRGASGYWV+NGVIQYPVEEITIAGNMK Sbjct: 361 ADTFKGMLKKMGTGLLVTELMGQGVNYVTGDYSRGASGYWVENGVIQYPVEEITIAGNMK 420 Query: 421 EMLRQIVAVGNDTLIRGSKETGSILLENMTIAGD 454 +MLRQIVAVGNDTLIRG+KETGSILLE+MTIAGD Sbjct: 421 DMLRQIVAVGNDTLIRGTKETGSILLESMTIAGD 454 >gi|300312124|ref|YP_003776216.1| Zn-dependent protease TLDE protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 328 bits (840), Expect = 6e-91, Method: Compositional matrix adjust. Identities = 175/444 (39%), Positives = 257/444 (57%), Gaps = 4/444 (0%) Query: 10 QDQLKQLAQDVLRYARDKGASDAAVDISEGSGLSVAVRKGKIETIEQNKDKGIGVTVFLG 69 Q + +AQ L AR GA+ AAV I E LS +V+ GK + + +++FLG Sbjct: 17 QQHFETIAQQTLEVARQHGATAAAVQIEEVESLSASVQGGKPNERALRQSAQLRLSLFLG 76 Query: 70 EGRQVRRGNASTSDFSQQALKETVEAAYNIARFTSIDDCAGLPEAEMLEMAPRDLKLFSP 129 + R + S+SD SQQ + V + A + D AGLP DL+L Sbjct: 77 Q----REASVSSSDLSQQGVAALVARGMDSAAIAAEDQHAGLPGLSAYPSDDVDLELDHA 132 Query: 130 WNISAEEAVALAQRCETAALETDARISNSEGAGVYVQHSHFIAANSRGFMGGYPFSRHTI 189 W + +AVAL R E AAL + + E A ++ + + ANS GF+ +RH++ Sbjct: 133 WQLGLRDAVALVARIERAALAVSSAVPQIEQAVLFSSRTRRVLANSEGFLRSSASTRHSL 192 Query: 190 SVAPIAGKGAAMQRDDWYSSKRDPKKLARPEAIGRYAAERALARLNARKLSTRKCPVLFE 249 S +A + A + + W+ ++RDP +L PE++G A RALARL AR+++T+ CPVLFE Sbjct: 193 SCTAVAIRDGAKEIESWWDARRDPAQLPSPESLGACAGRRALARLGARQVATQTCPVLFE 252 Query: 250 APLAAGLLGAFVQAVSGGALYRKSTFLLDSLGQQVFPEHIQILEDPHVIGGVGSAPFDEE 309 AA LL FVQA+S LY S+FL D+LGQ+VF H+ I +DP + GG+GS +D Sbjct: 253 PQAAASLLNEFVQAISARPLYMHSSFLRDALGQEVFAAHMNISDDPLLKGGMGSRSYDGN 312 Query: 310 GVRTVKREVVKDGVVQGYFLSTYSARKLGMQTTGNSGGSHNLTLTSELTTRADTFKGMLK 369 G R ++ G +QGY+L Y AR+LG+ + G N+ ++S + + + M++ Sbjct: 313 GAALRPRTLIAGGRLQGYYLGAYGARRLGLPLAEHGAGPSNVMVSSTTSAASGDLEAMIR 372 Query: 370 KMGTGLLVTELMGQGVNYVTGDYSRGASGYWVDNGVIQYPVEEITIAGNMKEMLRQIVAV 429 +MGTGL+++ L GQGVN +TGD+SR SG+WV+NG I YPV IT+A N++EM IVAV Sbjct: 373 RMGTGLVISGLNGQGVNLMTGDFSRACSGFWVENGQIAYPVAGITVASNLREMFAGIVAV 432 Query: 430 GNDTLIRGSKETGSILLENMTIAG 453 GND L + TGS L++ M + G Sbjct: 433 GNDRLTQNGTTTGSWLIDKMRVGG 456 >gi|300310755|ref|YP_003774847.1| Zn-dependent protease [Herbaspirillum seropedicae SmR1] Length = 486 Score = 64.3 bits (155), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 44/160 (27%), Positives = 77/160 (48%), Gaps = 7/160 (4%) Query: 280 LGQQVFPEHIQILEDPHVIGGVGSAPFDEEGVRTVKREVVKDGVVQGYFLSTYSARKLGM 339 +G++V + + +++D + GS D+EG T +++DG+++GY T +AR + M Sbjct: 290 IGERVAAKGVTVVDDGTIADRRGSLNIDDEGNPTQCTTLIEDGILKGYIQDTMNARLMKM 349 Query: 340 QTTGNSGGSHNLTLTSELTTRADTFKG------MLKKMGTGLLVTELMGQGVNYVTGDYS 393 TGN+ L T G +L + GL G V+ G + Sbjct: 350 DVTGNARRESFAHLPMPRMTNTYMLGGDRDPAEILASVKNGLYAVNFGGGQVDITNGKFV 409 Query: 394 RGAS-GYWVDNGVIQYPVEEITIAGNMKEMLRQIVAVGND 432 AS Y +++G I YPV+ T+ GN ++L ++ +GND Sbjct: 410 FSASEAYMIEDGKITYPVKGATLIGNGPDVLNRVSMIGND 449 >gi|300312123|ref|YP_003776215.1| Zn-dependent protease TldD protein [Herbaspirillum seropedicae SmR1] Length = 501 Score = 59.7 bits (143), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 45/172 (26%), Positives = 83/172 (48%), Gaps = 7/172 (4%) Query: 271 RKSTFLLDSLGQQVFPEHIQILEDPHVIGGVGSAPFDEEGVRTVKREVVKDGVVQGYFLS 330 R S+ +GQ+V + I++D V G GS D+EG +++DG++ Y Sbjct: 296 RGSSAFAGRIGQRVAAPGVTIVDDGTVPGSRGSLNIDDEGTPGQCTTLIEDGILVSYMQD 355 Query: 331 TYSARKLGMQTTGNS-GGSHNLTLTSELTTRADTFKG-----MLKKMGTGLLVTELMGQG 384 + +AR +G+ TGN S+++ +T G ++ + G+ + L G Sbjct: 356 SLNARLMGVANTGNGRRESYDVLPMPRMTNTYMRAGGVDPAEIIASVKQGIYIAHLEGGQ 415 Query: 385 VNYVTGDYSRGAS-GYWVDNGVIQYPVEEITIAGNMKEMLRQIVAVGNDTLI 435 V+ +G + AS + ++NG + PV+ T+ GN + L+ + +GND I Sbjct: 416 VDISSGRFMFAASEAFLIENGRLGAPVKGATVLGNGPQSLKNVKLIGNDLEI 467 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.132 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,388,962 Number of extensions: 59899 Number of successful extensions: 198 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 195 Number of HSP's successfully gapped: 4 Length of query: 454 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 366 Effective length of database: 1,203,190 Effective search space: 440367540 Effective search space used: 440367540 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)