BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1713.p (339 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311178|ref|YP_003775270.1| lipase [Herbaspirillum seropedi... 640 0.0 gi|300311659|ref|YP_003775751.1| esterase/lipase [Herbaspirillum... 131 6e-32 gi|300309659|ref|YP_003773751.1| esterase-lipase superfamily pro... 104 1e-23 >gi|300311178|ref|YP_003775270.1| lipase [Herbaspirillum seropedicae SmR1] Length = 340 Score = 640 bits (1652), Expect = 0.0, Method: Compositional matrix adjust. Identities = 318/340 (93%), Positives = 322/340 (94%), Gaps = 1/340 (0%) Query: 1 MKSFKQLAAVAAIGLVFGNAYAAGGPGVEPTTQAFLQALEAGGGKPLEQLSPKDARAVLV 60 M SFKQLAAVAAIGLVFGNAYAAG GVEPTTQAFLQALEAGGGKPLEQLSP DARAVLV Sbjct: 1 MTSFKQLAAVAAIGLVFGNAYAAGDAGVEPTTQAFLQALEAGGGKPLEQLSPADARAVLV 60 Query: 61 GAQAGVKLELPKADVSEKTIRVDGQDIKLTIVRPAG-VKKTLPAFMFFHGGGWVLGDFPT 119 GAQA VKL+LPKADVSEKTI DGQ IKLTIVRPAG KKTLPAFMFFHGGGWVLGDFPT Sbjct: 61 GAQASVKLQLPKADVSEKTISADGQQIKLTIVRPAGSAKKTLPAFMFFHGGGWVLGDFPT 120 Query: 120 HERLVRDLVANSGAVAVFVNYTPSPEASYGVAINQAYAATKWVAEHGKEINVDGKRLAVA 179 HERLVRDLVANSGAVAVFVNYTPSPEA YGVAINQAYAATKWVAEHGKEINVDGKRLAVA Sbjct: 121 HERLVRDLVANSGAVAVFVNYTPSPEAHYGVAINQAYAATKWVAEHGKEINVDGKRLAVA 180 Query: 180 GNSVGGNMAAVVALMAKDKGGPALRSEVLLWPVTDANFETGSYNQFANGYFLTKNMMKWF 239 GNSVGGNMAAVVALMAKDKGGPALRSEVLLWPVTDANFET SYNQFANGYFLTKNMMKWF Sbjct: 181 GNSVGGNMAAVVALMAKDKGGPALRSEVLLWPVTDANFETASYNQFANGYFLTKNMMKWF 240 Query: 240 WDSYTTDAAKRKEITASPLNATTEQLAGLPPTLIQTAENDVLRDEGEAYGRKLQAAGVAV 299 WDSYTTDAAKRKEITASPLNAT QL+GLPPTLIQTAE DVLRDEGE YGRKL AAGVAV Sbjct: 241 WDSYTTDAAKRKEITASPLNATPAQLSGLPPTLIQTAEKDVLRDEGEEYGRKLAAAGVAV 300 Query: 300 TSVRYNGMIHDFGLLNVLAKVPAVQSAMRQAGEELKYHLK 339 TSVRYNGMIHDFGLLNVLAKVPAVQ+AMRQAGEELK+ LK Sbjct: 301 TSVRYNGMIHDFGLLNVLAKVPAVQTAMRQAGEELKFRLK 340 >gi|300311659|ref|YP_003775751.1| esterase/lipase [Herbaspirillum seropedicae SmR1] Length = 317 Score = 131 bits (330), Expect = 6e-32, Method: Compositional matrix adjust. Identities = 94/295 (31%), Positives = 143/295 (48%), Gaps = 17/295 (5%) Query: 28 VEPTTQAFLQALEAG----GGKPLEQLSPKDARAVLVGAQAGVKLELPKADVSEKTI--- 80 + P AFL +EAG +P+ + S ARA A L+LP D++E+ Sbjct: 3 LHPDLDAFLALVEAGIQSGRQQPMHRKSVHQARADYEAA--APMLDLPPDDLAERRALEI 60 Query: 81 -RVDGQDI--KLTIVRPAGVKKTLPAFMFFHGGGWVLGDFPTHERLVRDLVANSGAVAVF 137 DG I +L P P ++FHGGG+ +G +H+ L L A + + Sbjct: 61 PTRDGARIGARLYAPAPLDAAAPQPVLLYFHGGGYCVGSLDSHDTLCSALAARTPCCVLA 120 Query: 138 VNYTPSPEASYGVAINQAYAATKWVAEHGKEINVDGKRLAVAGNSVGGN-MAAVVALMAK 196 V+Y +PE + A A A W+ +HG + + RLAV G+S GG AA+ + Sbjct: 121 VDYRLAPEHRFPTAFEDAADAYDWLLQHGPALGLATNRLAVGGDSAGGTLAAALALRARQ 180 Query: 197 DKGGPALRSEVLLWPVTDANFETGSYNQFANGYFLTKNMMKWFWDSYTTDAAKRKEITAS 256 P L +VLL+P T A+ + S+ +FA GY L ++W + +R++ + Sbjct: 181 AARAPCL--QVLLYPCTSAHQDGDSHRRFATGYLLEGETLQWMFGHTLNGDHERQDWRFA 238 Query: 257 PLNATTEQLAGLPPTLIQTAENDVLRDEGEAYGRKLQAAGVAVTSVRYNGMIHDF 311 PL A ++G P L+ ++D L DEG AY KL+ AGV V + GM+HDF Sbjct: 239 PLQAA--DVSGAAPALVVIGDHDPLLDEGMAYAEKLRQAGVPVQLDIHPGMVHDF 291 >gi|300309659|ref|YP_003773751.1| esterase-lipase superfamily protein [Herbaspirillum seropedicae SmR1] Length = 332 Score = 104 bits (259), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 73/210 (34%), Positives = 104/210 (49%), Gaps = 7/210 (3%) Query: 102 PAFMFFHGGGWVLGDFPTHERLVRDLVANSGAVAVF-VNYTPSPEASYGVAINQAYAATK 160 P M+FHGGG+V G + R +A G +AV V Y +P + A A+AA Sbjct: 84 PLVMYFHGGGFVSGSLEDADTPAR-FIAEHGQLAVLSVGYALAPAKPFPAAPEDAFAAAT 142 Query: 161 WVAEHGKEINVDGKRLAVAGNSVGGNMAAVVALMAKDKGGPALRSEVLLWPVTDANFE-T 219 W ++ E+ VD R+ VAG+ GG +AA + LM +D+ G L ++VL+ P+ D + Sbjct: 143 WACKNATELKVDLDRMVVAGDDAGGCLAASLTLMTRDRCGKPLAAQVLISPMLDPSMRLL 202 Query: 220 GSYNQFANGYFLTKNMMKWFWDSYTTDAAKRKEITASPLNATTEQLAGLPPTLIQTAEND 279 G + + LT + Y +R A+PL + +LAGLPP I TAE D Sbjct: 203 GDAARLQSD--LTAETCAASYRQYLPQTMQRLHPYAAPLEVS--RLAGLPPAFIATAERD 258 Query: 280 VLRDEGEAYGRKLQAAGVAVTSVRYNGMIH 309 VL E E Y L AGV R+ G+ H Sbjct: 259 VLCPEAEKYASSLIRAGVPTQVSRFAGITH 288 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.132 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,147,587 Number of extensions: 51381 Number of successful extensions: 206 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 202 Number of HSP's successfully gapped: 3 Length of query: 339 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 253 Effective length of database: 1,212,660 Effective search space: 306802980 Effective search space used: 306802980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)