BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1706.p (539 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313075|ref|YP_003777167.1| gamma-glutamyltranspeptidase [H... 244 1e-65 gi|300310422|ref|YP_003774514.1| gamma-glutamyltranspeptidase [H... 235 8e-63 >gi|300313075|ref|YP_003777167.1| gamma-glutamyltranspeptidase [Herbaspirillum seropedicae SmR1] Length = 527 Score = 244 bits (624), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 197/527 (37%), Positives = 269/527 (51%), Gaps = 26/527 (4%) Query: 26 GMVATSQPLAAQAGLEVLQRGGNAIDAAIAAAAALTVVEPTANGIGGDAFALVWHGGKLH 85 GMV+ LAAQAG ++L++GGNA++A +AAAA + VV P NGIGGD F L+ G+ Sbjct: 10 GMVSAPHHLAAQAGAQILRQGGNAVEAMVAAAATIAVVYPHMNGIGGDGFWLISEPGRAP 69 Query: 86 -GLNASGPAPQSISADKLTSAGLKEMPKYGALPVTVPGTPSAWAAM----AKRFGK-LTL 139 + A GPA + G + MP G A A A+RF L L Sbjct: 70 VAIRACGPAAALANKAFYAERGYRAMPTRGPHAALTVAGAVAGWAAALEHAQRFASGLPL 129 Query: 140 SDSMAGAITLAREGFPVSPMVAHAWQVAQQNFKRE-FKETHFQPWFD-TFCIDGQAPQAG 197 +A AI A+ G PV+ +Q R+ + E QP F T+ DG PQ G Sbjct: 130 PVLLADAIGHAQAGIPVT--------RSQHELTRDKWSELKDQPGFAATYAADGVPPQ-G 180 Query: 198 QLWRSAGHAATLQAIAEDGAESFYRGALAEQIAAFLQHAGGYLQAADLAAFQAQWVDPIG 257 ++ + + AA+L +AE G + FYRG LA+ +AA LQ AG L+ +DL AF+AQ V P+ Sbjct: 181 KVLKQSALAASLSRLAEAGLDDFYRGDLAQSLAAGLQAAGSPLRRSDLEAFRAQVVQPLM 240 Query: 258 VNYRGYEVWEIPPNGHGMVALMALNLLKAYDFTERDTLATYHRQIEAIKLAYADGLAHIA 317 V V+ +PP G+ ALM + L T+ D A H +EA K A+ H+ Sbjct: 241 VQLSSGRVYNLPPPTQGVSALMIIGLFDRLGVTQADGFAHLHGLVEATKRAFILRNQHVT 300 Query: 318 DPAHMKVTVQQ-LLSDAYADERRQLIGPRATLPVAGDPSRGGTVYLCTADAEGNMVSFIQ 376 DPAHM+V Q LL+ A E R + RA P G T+++ ADA+G +VS+IQ Sbjct: 301 DPAHMRVDAAQWLLAGALDQEVRHIDMQRAA-PWPHPARPGDTIWMGAADAQGRVVSYIQ 359 Query: 377 SNYMGFGSGLVVPGTGIALHNRGNNFTLEAGHSNVLAGGKRPYHTIIPGFMTRNGQAVGP 436 S + FGSG+VVP TGI NRG +FTL+ G N L GK P+HT+ P + Sbjct: 360 SIFWEFGSGVVVPDTGIVWQNRGASFTLDEG-VNQLQPGKLPFHTLNPSLAQLDDGRTLA 418 Query: 437 FGVMGGFMQPQGHVQMVMNTVDFGLNPQAALDAPRW----EW-EKGNKVSIEHTTAEHLF 491 FG MGG QPQ + V FG N QAA+ APRW W + + +E E Sbjct: 419 FGTMGGEGQPQTQAAVFTRHVLFGQNLQAAVSAPRWLLGRTWGDVSTNLKLEGRIPEATI 478 Query: 492 RGLGGLGHEVSWSGNQLG-FGRGQIIWRNDEGVLCGGTEPRTDGCVA 537 + L GHEV + G + + +G++ G T+PR DG A Sbjct: 479 QALRRAGHEVQVVDDYADMMGHAGAVCVHPDGLIEGATDPRADGVCA 525 >gi|300310422|ref|YP_003774514.1| gamma-glutamyltranspeptidase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 235 bits (599), Expect = 8e-63, Method: Compositional matrix adjust. Identities = 170/543 (31%), Positives = 268/543 (49%), Gaps = 44/543 (8%) Query: 19 SAVYARRGMVATSQPLAAQAGLEVLQRGGNAIDAAIAAAAALTVVEPTANGIGGDAFALV 78 S V+++ GMVAT Q LA + G+++L++GGNA+DA +A AL VV P A IGG F ++ Sbjct: 48 SPVHSQHGMVATEQELATKVGVDILKKGGNAVDAGVAVGFALAVVLPNAGNIGGGGFMMI 107 Query: 79 WHG--GKLHGLNASGPAPQSISADKLTSAGLKEMP---KYGALPVTVPGTPSAWAAMAKR 133 GK L+ APQ + + +P Y L V VPGT + + + Sbjct: 108 HDAKTGKNVALDFREMAPQRATRNMYLDDKGNVVPGRSLYTHLAVGVPGTVAGLSHALSK 167 Query: 134 FGKLTLSDSMAGAITLAREGFPVSPMVAHAWQVAQQNFKREFKETHFQPWFDT---FCID 190 +G + LS + A+ LA +G+PVS +A ++ H W + F + Sbjct: 168 YGTMKLSQVIQPAVELAEKGYPVSRSLAQILSAERE---------HLGQWDSSKAIFFKE 218 Query: 191 GQAPQAGQLWRSAGHAATLQAIAEDGAESFYRGALAEQIAAFLQHAGGYLQAADLAAFQA 250 G+ Q G+L A +L+ IA++G ++FY G++A++I A + GG + A DL ++ Sbjct: 219 GRPLQEGELLVQKDLAKSLRLIAKEGPKAFYEGSIAKKIVAEMDKHGGLISADDLKNYKV 278 Query: 251 QWVDPIGVNYRGYEVWEIPPNGHGMVALM-ALNLLKAYDFTER--DTLATYHRQIEAIKL 307 P+ NYRGY+V +PP G V ++ +N+L+ Y + D+ T H E +KL Sbjct: 279 VERVPVSGNYRGYQVMSMPPPSSGGVHIIQMMNILERYPLKQYGADSAQTIHLMAETMKL 338 Query: 308 AYADGLAHIADPAHMKVTVQQLLSDAYADERRQLIGPRATLPVA----GDP---SRGGTV 360 AYAD ++ DP KV V+ L S AYADE + I P P + G P T Sbjct: 339 AYADRSEYLGDPDFTKVPVKGLTSRAYADELAKKINPDRATPSSEIKPGQPQPYESDQTT 398 Query: 361 YLCTADAEGNMVSFIQSNYMGFGSGLVVPGTGIALHNRGNNFT----------LEAGHSN 410 + AD +GN+V+ + + FGSG+V GTGI L+N ++F L G +N Sbjct: 399 HYSVADKDGNLVATTYTLNLNFGSGIVATGTGITLNNEMDDFAAKPGVPNAFGLVGGDAN 458 Query: 411 VLAGGKRPYHTIIPGFMTRNGQAVGPFGVMGGFMQPQGHVQMVMNTVDFGLNPQAALDAP 470 +A KRP ++ P F+ ++G+ G GG +Q ++N +D +N A P Sbjct: 459 AVAPYKRPLSSMSPTFVLKDGKPFLVTGSPGGSRIITTTLQTILNVIDHDMNVAEATITP 518 Query: 471 RW--EWEKGNKVSIEHTTAEHLFRGLGGLGHEVSWSGNQLGFGRGQIIWRNDEGVLCGGT 528 R +W +++ +E + + L G ++S Q GR Q I +G G + Sbjct: 519 RIHHQWAP-DQLRVEKGLSADTLKILQDKGQKISV---QPSMGRTQTIQIRSDG-FDGFS 573 Query: 529 EPR 531 +PR Sbjct: 574 DPR 576 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,943,705 Number of extensions: 89766 Number of successful extensions: 257 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 249 Number of HSP's successfully gapped: 2 Length of query: 539 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 449 Effective length of database: 1,193,720 Effective search space: 535980280 Effective search space used: 535980280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)