BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1694.p (437 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 773 0.0 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 444 e-126 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 390 e-110 gi|300309876|ref|YP_003773968.1| major facilitator superfamily p... 345 4e-96 gi|300313342|ref|YP_003777434.1| major facilitator superfamily p... 338 7e-94 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 773 bits (1997), Expect = 0.0, Method: Compositional matrix adjust. Identities = 414/437 (94%), Positives = 425/437 (97%) Query: 1 MSYSPSAATTAAPPGSAAAPLEATYGKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD 60 MSYSPSAA T A SAAAPLE TY KIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD Sbjct: 1 MSYSPSAAATPAAHASAAAPLEVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD 60 Query: 61 LQFSEAVYGLGAGIFFIGYFLFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVSTPT 120 LQFSEAVYGLGAGIFFIGYF+FEVPSNLLLEKIGARKTLARITILWGLASMAMAYV+TPT Sbjct: 61 LQFSEAVYGLGAGIFFIGYFIFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPT 120 Query: 121 SFYVLRFLLGIFEAGFFPGVVLYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGFIMS 180 SFY+LRFLLGIFEAGFFPGVVLYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAG IMS Sbjct: 121 SFYILRFLLGIFEAGFFPGVVLYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMS 180 Query: 181 RMEGVGHLANWQWLFIIEGIPSILAGFAVLAWLPEKPANAKWLSEAEKNAVTQALALENS 240 MEGVGHLANWQWLFI+EGIPSILAGFAVLAWLPE+PANAKWLSEAEKNAVTQ LA ENS Sbjct: 181 GMEGVGHLANWQWLFILEGIPSILAGFAVLAWLPERPANAKWLSEAEKNAVTQVLAAENS 240 Query: 241 APGKHFSFKDACSNYRVWICAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIP 300 APGKHFSFKDAC+NYRVWICAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIP Sbjct: 241 APGKHFSFKDACTNYRVWICAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIP 300 Query: 301 FVAGTLAMIWNGFHSDKTGERRLHSAMATLIACIGLVATGMTLGNATLALCSLTLAAIGI 360 F+AGTLAMIWNGFHSDKTGERRLHSA+ATLIACIGLVATGMTLGNATLALCSLTLAAIGI Sbjct: 301 FIAGTLAMIWNGFHSDKTGERRLHSAIATLIACIGLVATGMTLGNATLALCSLTLAAIGI 360 Query: 361 LAAFPVFWSIPGAFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVA 420 LAAFPVFWSIPGAFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVA Sbjct: 361 LAAFPVFWSIPGAFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVA 420 Query: 421 ALEFLASFLVVVFVKKH 437 ALE +ASFLVVVF++KH Sbjct: 421 ALELVASFLVVVFIRKH 437 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 444 bits (1142), Expect = e-126, Method: Compositional matrix adjust. Identities = 215/400 (53%), Positives = 290/400 (72%), Gaps = 1/400 (0%) Query: 22 EATYGKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFL 81 +ATY K+ WRL+P L +V +++DRVNVGFAKLQMLQDL+FS+ VYGLGAGIFF+GYF+ Sbjct: 18 DATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYFV 77 Query: 82 FEVPSNLLLEKIGARKTLARITILWGLASMAMAYVSTPTSFYVLRFLLGIFEAGFFPGVV 141 FEVPSN++L K+GAR +ARI I WG+ S AMAYV+TP FY++RFLLG+ EAGFFPGV+ Sbjct: 78 FEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGVI 137 Query: 142 LYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGFIMSRMEGVGHLANWQWLFIIEGIP 201 LYLTYW+PA RR ++ LFMT A++G +GGP++G+IM M GV WQW+FI+E IP Sbjct: 138 LYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAIP 197 Query: 202 SILAGFAVLAWLPEKPANAKWLSEAEKNAVTQALALENSAPGKHFSFKDACSNYRVWICA 261 S++ G V+ ++ ++ +A WLSE EK + ++ E S +H S +N RVW+ A Sbjct: 198 SLILGVMVILYMKDRIRDADWLSEEEKRLLEANISHEESQK-EHLSLGAMFANGRVWLGA 256 Query: 262 AVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFVAGTLAMIWNGFHSDKTGER 321 +YFC V G ++FW P+IIK GV L +GL++AIP+ AM+ G +D ER Sbjct: 257 LIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADARRER 316 Query: 322 RLHSAMATLIACIGLVATGMTLGNATLALCSLTLAAIGILAAFPVFWSIPGAFLAGTAAA 381 R H A+ I CIGL+ + N TLA+ SLTLA++GIL P+FWS+P AFL+GTAAA Sbjct: 317 RWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSGTAAA 376 Query: 382 GGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAA 421 GIALINS+GNLAGFV+P+++G LK +T S ++G+Y +AA Sbjct: 377 AGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAA 416 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 390 bits (1003), Expect = e-110, Method: Compositional matrix adjust. Identities = 199/422 (47%), Positives = 279/422 (66%) Query: 6 SAATTAAPPGSAAAPLEATYGKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSE 65 +A+TT + Y K+ WR++PFL+ +V+A++DRVNVGFAKLQM QDL FSE Sbjct: 3 TASTTGVGLDVNDKSKDGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSE 62 Query: 66 AVYGLGAGIFFIGYFLFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVSTPTSFYVL 125 V+GLGAG+FFIGYFLFEVPSN+L+ K+GAR +ARI I WG+ S A +V T FY+L Sbjct: 63 TVFGLGAGLFFIGYFLFEVPSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYIL 122 Query: 126 RFLLGIFEAGFFPGVVLYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGFIMSRMEGV 185 RFLLG+ EAGF+PG++LYLTYWFP+ RRA+V +FM+ +AG +G P++G+IM Sbjct: 123 RFLLGLAEAGFYPGIILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQN 182 Query: 186 GHLANWQWLFIIEGIPSILAGFAVLAWLPEKPANAKWLSEAEKNAVTQALALENSAPGKH 245 G L WQW+F+IE IP++L G A + +L +AKWL++ EK ++ + + Sbjct: 183 GGLEGWQWMFLIEAIPAVLIGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKESK 242 Query: 246 FSFKDACSNYRVWICAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFVAGT 305 F + RVW+ +YF V G + W P+++K GVKGNL+IGL+SAIPF Sbjct: 243 HGFGAIVKDARVWLMCLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAI 302 Query: 306 LAMIWNGFHSDKTGERRLHSAMATLIACIGLVATGMTLGNATLALCSLTLAAIGILAAFP 365 +AM G +D+ ERR H + L+ +G V + N +++ SL+LAA G+L P Sbjct: 303 IAMNLIGRSADRMRERRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAP 362 Query: 366 VFWSIPGAFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALEFL 425 +FWS+P AFL+G AAA GIA INS+GNLAGFV+PY+IG LK T + ++G+Y +A + + Sbjct: 363 LFWSLPTAFLSGAAAAVGIAAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYMLAVMLVV 422 Query: 426 AS 427 S Sbjct: 423 GS 424 >gi|300309876|ref|YP_003773968.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 449 Score = 345 bits (885), Expect = 4e-96, Method: Compositional matrix adjust. Identities = 183/418 (43%), Positives = 264/418 (63%), Gaps = 5/418 (1%) Query: 1 MSYSPSAATTAAPPGSAAAPLEATYGKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD 60 M+ S AT + P A+ +A Y K+ R+IPFL +V++++DR+N+GFA+LQM QD Sbjct: 1 MTPSSGGATLSTTPSLASQAEDAIYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMKQD 60 Query: 61 LQFSEAVYGLGAGIFFIGYFLFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVSTPT 120 L FS+A+YGLGA IF++GY LFEVPSN+ L + GAR+T +RI +LWGLAS+ M V+ Sbjct: 61 LGFSDAMYGLGAAIFYVGYVLFEVPSNMALARFGARRTFSRIMVLWGLASVGMMAVTDAA 120 Query: 121 SFYVLRFLLGIFEAGFFPGVVLYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGFIMS 180 FY LRFLLG+FEAGFFPG+VLYL+YWFPA+RRA V +F A+AG +GG ++G+IM Sbjct: 121 HFYTLRFLLGVFEAGFFPGIVLYLSYWFPARRRAAVMAIFFAGVAVAGVLGGLVSGWIMR 180 Query: 181 RMEGVGHLANWQWLFIIEGIPSILAGFAVLAWLPEKPANAKWLSEAEK---NAVTQALAL 237 M GV L WQW+F IEG P+++ G + L + PA AKWLS E+ A Q AL Sbjct: 181 DMAGVMGLYGWQWMFAIEGAPAVVLGLLAIFLLADGPAQAKWLSPEERSFLQARHQQEAL 240 Query: 238 ENSAPGKHF--SFKDACSNYRVWICAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGL 295 H + + A S+ ++ A +YF + G+ T+ FW P +I++ G+K +++ L Sbjct: 241 RTHHVSGHSLAALRQALSHRTTYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVVEVSL 300 Query: 296 ISAIPFVAGTLAMIWNGFHSDKTGERRLHSAMATLIACIGLVATGMTLGNATLALCSLTL 355 S IP G + +I SD+ ER H A+ T + L A + + ++L L++ Sbjct: 301 YSVIPNAIGAVGLILIARRSDRRNERHGHFAVCTTGGALALAALTLHPSSFAVSLGLLSV 360 Query: 356 AAIGILAAFPVFWSIPGAFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLS 413 AA+ I AA P+FWS+P L A GIA I+S+G +G V+P++IG +KT TG++ Sbjct: 361 AAVLIFAALPIFWSLPPGLLPAQTIAPGIAFISSVGITSGIVSPWLIGLVKTRTGNMD 418 >gi|300313342|ref|YP_003777434.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 338 bits (866), Expect = 7e-94, Method: Compositional matrix adjust. Identities = 190/430 (44%), Positives = 268/430 (62%), Gaps = 10/430 (2%) Query: 17 AAAPLEATYGKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFF 76 +A + Y K+ W+++P+L+ +V+A++DRVNVGFAKL ML DL SEA YGLGAGIFF Sbjct: 8 SAVQFDGLYKKVTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAYGLGAGIFF 67 Query: 77 IGYFLFEVPSNLLLEKIGARKTLARITILWGLASMAMAY---------VSTPTSFYVLRF 127 +GYF+FEVPSNL+L ++GA+ +ARI I WG+ S MA+ VS T FY LRF Sbjct: 68 LGYFIFEVPSNLILHRVGAKIWIARIMITWGMISSLMAFTAPIAQWLGVSNETVFYTLRF 127 Query: 128 LLGIFEAGFFPGVVLYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGFIMSRMEGVGH 187 LLG EAGFFPGV+LY+TYW+PA +RV + + + +GGPI+G +M M Sbjct: 128 LLGASEAGFFPGVILYMTYWYPAAMHSRVMAVVLLGNPFSYILGGPISGALMQFMGSGDS 187 Query: 188 LANWQWLFIIEGIPSILAGFAVLAWLPEKPANAKWLSEAEKNAVTQALALENSAPGKHFS 247 LA WQW+F+IE +P+ L G +L L A+AKWL+ EK + + LA EN + Sbjct: 188 LAGWQWMFVIEALPACLLGIVILFVLSNDIASAKWLAPEEKAELARRLAAENKEK-VNVP 246 Query: 248 FKDACSNYRVWICAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFVAGTLA 307 K + +W A VY ++ G+ I FWAP+II++ GVK L IG+ISAIP++ G A Sbjct: 247 LKSLYALPILWFLALVYLLMMIGSYGINFWAPTIIRDAGVKSVLDIGMISAIPYILGATA 306 Query: 308 MIWNGFHSDKTGERRLHSAMATLIACIGLVATGMTLGNATLALCSLTLAAIGILAAFPVF 367 M++ H++K G+ R +S +++A + LV + N + L + +A G L + +F Sbjct: 307 MVFITRHAEKAGKHRSYSVAVSMVAGLALVLCTLFPHNIYITLAGIAIAVGGSLTSIALF 366 Query: 368 WSIPGAFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALEFLAS 427 W PGA+ G AA GIA+INS+GNL GFV PY++GAL T+ GS GL + FLA Sbjct: 367 WGFPGAYFTGATAAAGIAIINSVGNLGGFVGPYLLGALTTAFGSPRYGLMMLGGALFLAG 426 Query: 428 FLVVVFVKKH 437 L+ + +K Sbjct: 427 VLIALGGRKR 436 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.139 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,154,663 Number of extensions: 43054 Number of successful extensions: 302 Number of sequences better than 1.0e-08: 22 Number of HSP's gapped: 276 Number of HSP's successfully gapped: 22 Length of query: 437 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 349 Effective length of database: 1,203,190 Effective search space: 419913310 Effective search space used: 419913310 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)