BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1692.p (450 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311162|ref|YP_003775254.1| amidase family protein [Herbasp... 798 0.0 gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Her... 137 2e-33 gi|300313942|ref|YP_003778034.1| Asp-tRNAAsn/Glu-tRNAGln amidotr... 122 4e-29 gi|300310266|ref|YP_003774358.1| amidase family protein [Herbasp... 105 5e-24 gi|300313752|ref|YP_003777844.1| Asp-tRNAAsn/Glu-tRNAGln amidotr... 103 3e-23 >gi|300311162|ref|YP_003775254.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 450 Score = 798 bits (2062), Expect = 0.0, Method: Compositional matrix adjust. Identities = 414/450 (92%), Positives = 428/450 (95%) Query: 1 MPSTLSQLSAELAAGHITSLQLTEQALARIADPAGEGARVFTSVYAEQARAAARASDTLR 60 MPSTLS+L+A+LAAGH TSLQLTEQALARIADPAGEGARVFT VYAEQARAAARASDTLR Sbjct: 1 MPSTLSRLAADLAAGHTTSLQLTEQALARIADPAGEGARVFTRVYAEQARAAARASDTLR 60 Query: 61 AAGLCRSPLEGVPVSVKDLFDVAGETTLAGSTVLRGKPAASVHAAVVQNLLKAGAIVIGK 120 AA LCRSPLEGVPVSVKDLFDVAGETTLAGS VLRGKP AS HAAVVQNLLKAGAI+IGK Sbjct: 61 AARLCRSPLEGVPVSVKDLFDVAGETTLAGSVVLRGKPVASAHAAVVQNLLKAGAILIGK 120 Query: 121 TNMTEFAYSGLGINPHYGTPQNTWERNVEGGRIPGGSSSGAAISVTDGMACAAVGSDTGG 180 TNMTEFAYSGLGINPHYGTPQN WERN+EGGRIPGGSSSGAAISVTDGMA AAVGSDTGG Sbjct: 121 TNMTEFAYSGLGINPHYGTPQNAWERNIEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGG 180 Query: 181 SVRIPSALNGLTGFKPTAARVSMQGVLPLSAYLDSIGPLAVSVQCCAIMDAVLAGEEYVA 240 SVRIPSALNGLTGFKPTAARVSMQGVLPLSAYLDSIGPLAVSVQCCAIMDAVLAGE+YVA Sbjct: 181 SVRIPSALNGLTGFKPTAARVSMQGVLPLSAYLDSIGPLAVSVQCCAIMDAVLAGEDYVA 240 Query: 241 VTPHPLRGLRLLAPTNVVRDGMDDTVAVAYQTALARLSAAGAVIVEEAVPAFNQLAAINA 300 V HPLRGLRLLAPTNVV DGMDDTV AY+TAL +L+AAGA+IVE VPAFN LAAINA Sbjct: 241 VLAHPLRGLRLLAPTNVVLDGMDDTVGQAYRTALGKLAAAGAMIVEAEVPAFNALAAINA 300 Query: 301 KGGFTAAEAYAWHRALIAENAAGYDQRVVSRILRGKDMSAADLLDVLHARPRWIAEVEAQ 360 KGGFTAAEAYAWHRALIAENAAGYDQRVVSRILRGKDMSAADLLDVLHARPRWIA+VEAQ Sbjct: 301 KGGFTAAEAYAWHRALIAENAAGYDQRVVSRILRGKDMSAADLLDVLHARPRWIAQVEAQ 360 Query: 361 LAGYDALVMPTVPIVAPRIAELQASDEAYYAANGKLLRNPTLINFLDGCAVSLPCHAAGS 420 LAGYDALVMPTVPIVAPRIAELQASD+AYY ANGK+LRNPTLINFLDGCA+SLPCHA GS Sbjct: 361 LAGYDALVMPTVPIVAPRIAELQASDDAYYGANGKMLRNPTLINFLDGCALSLPCHAPGS 420 Query: 421 APVGLSLAAAGGQDKRLLSIALAVEGLLAA 450 APVGLSLAA GGQDKRLLSI LAVE LLAA Sbjct: 421 APVGLSLAACGGQDKRLLSIGLAVEALLAA 450 >gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Herbaspirillum seropedicae SmR1] Length = 468 Score = 137 bits (345), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 140/432 (32%), Positives = 207/432 (47%), Gaps = 52/432 (12%) Query: 47 EQARAAARASDTLRAAGLCRSPLEGVPVSVKDLFDVAGETTLAGSTVLRGKPAASVHAAV 106 EQ R+ AR D G PL G+P+++KD DVAG + AG+ LR HA V Sbjct: 48 EQLRSQARMLDEQGGHG----PLSGIPIAIKDNIDVAGIASTAGTAALRNN-VPQRHATV 102 Query: 107 VQNLLKAGAIVIGKTNMTEFAYSGLGINPHYGTPQNTWERNVEGGRIPGGSSSGAAISVT 166 V+ L AGA+ GK NM E A+ N +G +N ++ + I GGSS G + Sbjct: 103 VEYLFGAGALHAGKANMHELAFGITNNNGVFGPCRNPYDAEL----IAGGSSGGCGALLA 158 Query: 167 DGMACAAVGSDTGGSVRIPSALNGLTGFKPTAARVSMQGVLPLSAYLDSIGPLAVSVQCC 226 +A AAVG+DTGGSVRIP+AL G+ G +PT+ R S GV+P+S D+ GP+A SV Sbjct: 159 ARLAPAAVGTDTGGSVRIPAALCGVVGLRPTSRRYSQHGVVPISHTRDTPGPMARSVADV 218 Query: 227 AIMDAVLAGEEYVA-VTPHPLRGLRLLAPTNVVRDGMDDTVAVAYQTALARLSAAGAVIV 285 A++D ++AG A + P LRL N G+++ V AL +L AG +V Sbjct: 219 ALLDQIMAGSRDSAPLLALPPSQLRLGVLRNPSWQGLEEEVEANANAALMQLREAGVELV 278 Query: 286 EEAVPAFNQLAAINAKGGFTAAEAYAWHRALI---AENAAGYDQRVVSRILRGKDMSAAD 342 + +P LA ++ GF A H LI A A + V+ R+L ++ + D Sbjct: 279 DVDLPT---LAPLSHAAGF----AIVLHEFLIDLPAYLQASDARDVIKRVL--AEVGSPD 329 Query: 343 LLDVLHAR-PRWIAEVEAQLA------------------GYDALVMPTVPIVAPRIAELQ 383 + ++ A + + E +LA G DAL+ PT + A + E Sbjct: 330 VARIIQATLDQPVPEHAYRLALEQRRTMQALYRDCFRSQGVDALIFPTTALTARPLGE-- 387 Query: 384 ASDEAYYAANGKLLRNPTLINFLDGC------AVSLPCHAAGSA-PVGLSLAAAGGQDKR 436 D+ ++ P DG ++S+P + PVGL L G D+R Sbjct: 388 --DDTVALRGERVPTFPAFARNADGASIAGLPSISIPVGLSEYGLPVGLELDGPEGSDRR 445 Query: 437 LLSIALAVEGLL 448 LL +A ++E +L Sbjct: 446 LLQVAASLETVL 457 >gi|300313942|ref|YP_003778034.1| Asp-tRNAAsn/Glu-tRNAGln amidotransferase subunit A [Herbaspirillum seropedicae SmR1] Length = 488 Score = 122 bits (307), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 141/482 (29%), Positives = 217/482 (45%), Gaps = 57/482 (11%) Query: 4 TLSQLSAELAAGHITSLQLTEQALARIADPAGEGARVFTSVYAEQARAAARASDTLRAAG 63 TL +LSA+L A I++ +L L RI D + A F V E A A+ +D+ R + Sbjct: 5 TLKELSAQLQARKISATELATLFLDRI-DASDLNA--FLHVDRELTLAQAKVADS-RLSD 60 Query: 64 LCRSPLEGVPVSVKDLFDVAGETTLAGSTVLRGKPAASVHAAVVQNLLKAGAIVIGKTNM 123 L GVP++ KD+F G T AGS +L + A VV+ AG + +GK N Sbjct: 61 NNAGQLTGVPIAHKDIFVTRGWRTTAGSRMLDNY-VSPFDATVVEQFNAAGMVTLGKLNC 119 Query: 124 TEFAYSGLGINPHYGTPQNTWERNVEGGRIPGGSSSGAAISVTDGMACAAVGSDTGGSVR 183 EFA N ++G N W++ G SS G+A +V +A AA +DTGGS+R Sbjct: 120 DEFAMGSGNENSYFGPTLNPWDKRAVPGG----SSGGSAAAVAARLAPAATATDTGGSIR 175 Query: 184 IPSALNGLTGFKPTAARVSMQGVLPLSAYLDSIGPLAVSVQCCAIMDAVLAG-------- 235 P++L G+TG KPT VS G++ ++ LD GP+A + + C ++ + G Sbjct: 176 QPASLCGVTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAMTGFDERDSTS 235 Query: 236 -----EEYVAVTPHPLRGLRLLAPTNVVRDGMDDTVAVAYQTALARLSAAGAVIV----- 285 E++ L+GLR+ P G+ V A + AL+ GA +V Sbjct: 236 LQRPKEDFTRSLNDSLQGLRIGVPKEFFGAGLAADVEAAVRAALSEFEKLGAQLVEISLP 295 Query: 286 --EEAVPAFNQLAAINAKGGFTAAEAYAW-HRALIAEN---------AAGYDQRVVSRIL 333 E ++P + +A A + + + HRA N A G+ + V RIL Sbjct: 296 KTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAADYGNLDDMYRKSRAEGFGEEVKRRIL 355 Query: 334 RGKDMSAADLLDVLHARPRWIAEVEAQ-----LAG----YDALVMPTVPIVAPRIAELQA 384 G + + D + + + I + AQ LAG D ++ P P VA + + Sbjct: 356 VGSYVLSHGYYDAYYLQAQKIRRLIAQDFQNALAGANRQCDVIMGPVSPTVAWDLGDKTN 415 Query: 385 SDEAYYAANGKLLRNPTLINFLDGCAVSLPC-----HAAGSAPVGLSLAAAGGQDKRLLS 439 A Y A+ + T + L G +S+PC G PVGL + + RLL+ Sbjct: 416 DPVANYLAD--IFTLSTSLAGLPG--MSIPCGFGQGEKNGKRPVGLQIIGNYFDEARLLN 471 Query: 440 IA 441 +A Sbjct: 472 VA 473 >gi|300310266|ref|YP_003774358.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 105 bits (263), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 124/420 (29%), Positives = 171/420 (40%), Gaps = 42/420 (10%) Query: 63 GLCRSPLEGVPVSVKDLFDVAGETTLAGSTVLRGKPAASVHAAVVQNLLKAGAIVIGKTN 122 G L G V +KD +AG + GS+ L G S A +V LL AGA ++GK Sbjct: 82 GAAEGKLAGRTVVLKDNVALAGVPMMNGSSTLEGF-VPSYDATIVTRLLDAGATILGKAT 140 Query: 123 MTEFAYSGLGINPHYGTPQNTWERNVEGGRIPGGSSSGAAISVTDGMACAAVGSDTGGSV 182 F SG H P G G SS AA+ V G A A+G D GGS+ Sbjct: 141 CEHFCLSG---GSHTSDPAPVHNPRRHGYGSGGSSSGSAAL-VAAGEADMAIGGDQGGSI 196 Query: 183 RIPSALNGLTGFKPTAARVSMQGVLPLSAYLDSIGPLAVSVQCCAIMDAVLAG------- 235 RIPSA G G K T V GV+P+ A +D +GP+ +V+ A+M V+AG Sbjct: 197 RIPSAFCGTYGMKATHGLVPYTGVMPIEATVDHVGPITANVRDNALMLEVIAGADGLDPR 256 Query: 236 ------EEYVAVTPHPLRGLRL-LAPTNVVRDGMDDTVAVAYQTALARLSAAGAVIVEEA 288 + Y A +RGL++ + MD VA Q A+A+L GA + + Sbjct: 257 QYAPEVQPYTAALDRGVRGLKIGILTEGFALGNMDKRVADKVQEAIAQLEKLGASVATVS 316 Query: 289 VPAFNQLAAINAKGGFTAAEAYAWHRALIAENAAG-YD----QRVVSRILRGKDMSAADL 343 V A+ G H + N G YD Q D+SA Sbjct: 317 VEEHALAGALWQPIGCEGLTMQMMHGNGMGFNWKGLYDLGLLQHHAHWREHADDLSATLK 376 Query: 344 LDV-------------LHARPRWIAE-----VEAQLAGYDALVMPTVPIVAPRIAELQAS 385 L + +A+ + IA + LA +D LVMPTVPI+A + Sbjct: 377 LCMFVGQYGLTRYQGRFYAKAQNIARRARAGYDKALADFDLLVMPTVPIIAQPLPAAGCP 436 Query: 386 DEAYYAANGKLLRNPTLINFLDGCAVSLPCHAAGSAPVGLSLAAAGGQDKRLLSIALAVE 445 Y A +++ N ++ A+S+PC PVGL L + L A A E Sbjct: 437 LAEYVARAFEMIGNTAPLDITGHPAMSIPCGTVDGLPVGLMLVGKHYAEATLYQAAAAFE 496 >gi|300313752|ref|YP_003777844.1| Asp-tRNAAsn/Glu-tRNAGln amidotransferase subunit A [Herbaspirillum seropedicae SmR1] Length = 471 Score = 103 bits (257), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 117/395 (29%), Positives = 175/395 (44%), Gaps = 29/395 (7%) Query: 68 PLEGVPVSVKDLFDVAGETTLAGSTVLRGKPAASVHAAVVQNLLKAGAIVIGKTNMTEFA 127 PL GVP S+KD D+AG T A P S A +V L+ GAI +GK N+ +FA Sbjct: 69 PLYGVPFSIKDNIDLAGVPTTAACPAFSYVPRES--ATLVAQLIALGAIPVGKCNLDQFA 126 Query: 128 YSGLGINPHYGTPQNTWERNVEGGRIPGGSSSGAAISVTDGMACAAVGSDTGGSVRIPSA 187 G YG +N+ V GGSSSG++++V G++ A+G+DT GS R+P+A Sbjct: 127 TGLNGTRSPYGACRNS----VHPDYPSGGSSSGSSLAVALGLSSFALGTDTAGSGRVPAA 182 Query: 188 LNGLTGFKPTAARVSMQGVLPLSAYLDSIGPLAVS-------VQCCAIMDAVLAGEEYV- 239 LN L G K T +S GV+P LD + + + C +EY Sbjct: 183 LNNLVGLKATKGLLSTAGVVPACRTLDCVTFFTATAAEASHLLALCTQSGQPDERDEYSR 242 Query: 240 AVTPH--------PLRGLRLLAPTNVVRDGMDDTVAVAYQTALARLSAAGAVIVEEAVPA 291 A PH P +GLR P + G ++ ++ + ALA++ A G V V Sbjct: 243 ANLPHNDASAFQPPPQGLRFGVPRQLEFLGCSESPSL-FVEALAQMEAIGGVAVPIDFSP 301 Query: 292 FNQLAAINAKGGFTAAEAYAWHRALIAENAAGYDQRVVSRILRGKDMSAADLLDVLHARP 351 F A + +G + AE Y+ AL+ E + + + SA D + Sbjct: 302 FLAAARLLYEGPWV-AERYSTIEALLQERPDDILPVIRDVLRKAPQYSAVDTFRAQYRLQ 360 Query: 352 RWIAEVEAQLAGYDALVMPTVPIVAPRIAELQASDEAYYAANGKLLRNPTLINFLDGCAV 411 + AE + LA D +V P PR L+ E N L +N LD AV Sbjct: 361 QLKAECDRILATVDCVVTPAY----PRPVTLEELREQPVQRNSDLGWYTNFMNMLDYAAV 416 Query: 412 SLPCHAAGSA-PVGLSLAAAGGQDKRLLSIALAVE 445 + P + + P G+++ D+ LL +A A++ Sbjct: 417 ATPMNFMKNGLPWGITVFGRAFSDQALLGVADALQ 451 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.131 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,381,238 Number of extensions: 57806 Number of successful extensions: 294 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 269 Number of HSP's successfully gapped: 11 Length of query: 450 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 362 Effective length of database: 1,203,190 Effective search space: 435554780 Effective search space used: 435554780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)