BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1683.p (375 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311158|ref|YP_003775250.1| multidrug ABC transporter perme... 648 0.0 gi|300311268|ref|YP_003775360.1| multidrug ABC transporter perme... 415 e-117 gi|300311269|ref|YP_003775361.1| multidrug ABC transporter ATPas... 88 1e-18 gi|300311157|ref|YP_003775249.1| multidrug ABC transporter ATPas... 82 5e-17 >gi|300311158|ref|YP_003775250.1| multidrug ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 375 Score = 648 bits (1671), Expect = 0.0, Method: Compositional matrix adjust. Identities = 347/375 (92%), Positives = 358/375 (95%) Query: 1 MRNSWANIYRLGIKELWSLLRDPAMLVLIAYTFTLSIYVAATAMPESLHNAAIAIVDNDG 60 MRNSWANIYRLGIKELWSLLRDPAMLVLIAYTF+LSIYVAATAMPESLH+AAIAIVDND Sbjct: 1 MRNSWANIYRLGIKELWSLLRDPAMLVLIAYTFSLSIYVAATAMPESLHHAAIAIVDNDS 60 Query: 61 SPLSARIASAFYPPHFKVAQLVTAKQADAGLDAGDYTFVLDIPADFQRDVLAGRAPAVQL 120 SPLSARIA++FYPPHFKVA+LVTA+QADAGLD GDYTFVLDIPADFQRDVLA RAPAVQL Sbjct: 61 SPLSARIAASFYPPHFKVARLVTARQADAGLDDGDYTFVLDIPADFQRDVLAARAPAVQL 120 Query: 121 NVDATRMSQAFTGNSYIQQMVSNEVTQFVGRTEVTGSTPVGLALRMRFNPNLEQSWFGSL 180 NVDATRMSQAFTGNSYIQQMV+NEV QFV R E S PV LALRMRFNPNLEQSWFGSL Sbjct: 121 NVDATRMSQAFTGNSYIQQMVTNEVAQFVQRQEGGASAPVNLALRMRFNPNLEQSWFGSL 180 Query: 181 MEIINNVTMLSIILTGAALIREREHGTIEHLLVMPVSPAEIMLAKVWSMGAVVALAALAS 240 MEIINNVTMLSIILTGAALIREREHGTIEHLLVMPV+PAEIMLAKVWSMG VVALAALAS Sbjct: 181 MEIINNVTMLSIILTGAALIREREHGTIEHLLVMPVTPAEIMLAKVWSMGVVVALAALAS 240 Query: 241 LLLIVRGALQVPIEGSIALFMLATVLHLFATTSMGIFMATLARSMPQFGMLTVLVLLPLQ 300 LLLIVRGAL VPIEGS+ALFML T LHLFATTSMGIFMATLARSMPQFGMLTVLVLLPLQ Sbjct: 241 LLLIVRGALHVPIEGSVALFMLVTCLHLFATTSMGIFMATLARSMPQFGMLTVLVLLPLQ 300 Query: 301 LLSGGSTPRESMPVPVQDIMLAAPTTHFVAAGQAILYRGAGLEVVWPQLLAIFGIGLVLF 360 LLSGGSTPRESMPV VQDIMLAAPTTHFVAAGQAILYRGAGLEV+WPQLLAIFGIG VLF Sbjct: 301 LLSGGSTPRESMPVLVQDIMLAAPTTHFVAAGQAILYRGAGLEVIWPQLLAIFGIGAVLF 360 Query: 361 VAALGRFRKTISQMA 375 +AALGRFRKTISQMA Sbjct: 361 MAALGRFRKTISQMA 375 >gi|300311268|ref|YP_003775360.1| multidrug ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 375 Score = 415 bits (1067), Expect = e-117, Method: Compositional matrix adjust. Identities = 217/374 (58%), Positives = 280/374 (74%) Query: 1 MRNSWANIYRLGIKELWSLLRDPAMLVLIAYTFTLSIYVAATAMPESLHNAAIAIVDNDG 60 MR AN+++L +KELWSL RD ML LI +FT +Y AA+A+P++L +AAIAIVD D Sbjct: 1 MRQGLANLWQLTLKELWSLWRDRVMLCLIVLSFTGMVYSAASAIPDTLSHAAIAIVDEDD 60 Query: 61 SPLSARIASAFYPPHFKVAQLVTAKQADAGLDAGDYTFVLDIPADFQRDVLAGRAPAVQL 120 SPLS R+A+AF P F Q ++ + DAGLDAG Y FVL PA Q + G+ VQL Sbjct: 61 SPLSQRLAAAFMAPQFSPPQRISVAEMDAGLDAGRYVFVLHFPAGLQSKLQTGKPAEVQL 120 Query: 121 NVDATRMSQAFTGNSYIQQMVSNEVTQFVGRTEVTGSTPVGLALRMRFNPNLEQSWFGSL 180 NVDATRM QA++G++Y++Q++ E+ +F+ + + LALR RFNP LE++WF S+ Sbjct: 121 NVDATRMDQAYSGSAYVEQILQLEIRRFLQKRQDEPREAATLALRARFNPALEKTWFSSV 180 Query: 181 MEIINNVTMLSIILTGAALIREREHGTIEHLLVMPVSPAEIMLAKVWSMGAVVALAALAS 240 ++II+N+TML+IIL GAALIREREHGTIEHLLVMPVSP +IM AKV +MG VV +AA S Sbjct: 181 LQIIDNITMLAIILAGAALIREREHGTIEHLLVMPVSPVQIMGAKVLAMGGVVLVAATLS 240 Query: 241 LLLIVRGALQVPIEGSIALFMLATVLHLFATTSMGIFMATLARSMPQFGMLTVLVLLPLQ 300 L +VRG L VPIEGS+ LF+ L LFA T++GI +AT+ARSMPQFG+L +LVLLPL Sbjct: 241 LHGVVRGLLDVPIEGSVGLFLFGAALSLFANTALGISLATVARSMPQFGLLLMLVLLPLI 300 Query: 301 LLSGGSTPRESMPVPVQDIMLAAPTTHFVAAGQAILYRGAGLEVVWPQLLAIFGIGLVLF 360 LLSGG+TPRESMPV +Q +MLAAP+TH V G+ +LYRGAGLE+VWPQLLA+F IG +L Sbjct: 301 LLSGGATPRESMPVLIQYLMLAAPSTHLVRLGEGVLYRGAGLEMVWPQLLALFLIGNILL 360 Query: 361 VAALGRFRKTISQM 374 +L R R ++QM Sbjct: 361 WVSLRRLRTMLTQM 374 >gi|300311269|ref|YP_003775361.1| multidrug ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 913 Score = 87.8 bits (216), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 96/352 (27%), Positives = 159/352 (45%), Gaps = 17/352 (4%) Query: 4 SWANIYRLGIKELWSLLRDPAMLVLIAYTFTLSIYVAATAMPESLHNAAIAIVDNDGSPL 63 SW + +E L RDP + L ++V + ++ A++D D S Sbjct: 542 SWQRMLSYAWREALELRRDPVRATMALLGSLLLMFVIGYGISTDVNGLPYAVLDQDQSSA 601 Query: 64 SARIASAFY-PPHFKV-AQLVTAKQADAGLDAGDYTFVLDIPADFQRDVLAGRAPAVQLN 121 S A+A P+F+ A L Q D + G+ T ++IP+ F R + AGR + Sbjct: 602 SRDYAAALRASPYFQERAPLQGPAQLDKRMRNGELTLAIEIPSGFGRRLAAGRQVEIGAW 661 Query: 122 VDATRMSQAFTGNSYIQQMVSNEVTQFVGRTEVTGSTP-VGLALRMRFNPNLEQSWFGSL 180 ++ S+A T +Y+Q M + + Q R + TP + + R+R+N +L +S L Sbjct: 662 INGAMPSRAETVQAYVQGMHQHWLEQRQQRLQGRPQTPALEIQTRLRYNADL-RSLPAIL 720 Query: 181 MEIINNVTMLSIILTGA-ALIREREHGTIEHLLVMPVSPAEIMLAKVWSMGAVVALAALA 239 +I + + + A A++RE+E G I + V PV+ E + K +AL Sbjct: 721 PAVIPLLLLTLPAMLTALAVVREKELGAIINFYVTPVTRLEFLCGKQLP---YIALGMFN 777 Query: 240 SLLLIVRGA--LQVPIEGSIALFMLATVLHLFATTSMGIFMATLARSMPQFGMLTVL-VL 296 LL+ + L++P++GS A LAT+++L T MG+ + L RS +L L Sbjct: 778 FLLMSMAAVTLLELPVKGSYAALALATLVYLVIATGMGLVASALTRSQIAAMFAALLGTL 837 Query: 297 LPLQLLSGGSTP---RESMPVPVQDIMLAAPTTHFVAAGQAILYRGAGLEVV 345 +P +G +TP E M V DI P TH + + + GL V Sbjct: 838 VPATQFAGMTTPVSALEGMGRLVGDIH---PATHMFLISRGVYVKALGLADV 886 >gi|300311157|ref|YP_003775249.1| multidrug ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 923 Score = 82.4 bits (202), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 87/378 (23%), Positives = 168/378 (44%), Gaps = 28/378 (7%) Query: 2 RNSWANIYRLGIKELWSLLRDPAMLVLIAYTFTLSIYVAATAMPESLHNAAIAIVDNDGS 61 R S +E L RDP L + +++ + + N + A++D D + Sbjct: 550 RTSLGRALSYSQRESLELRRDPVRATLALLGTAILMFIIGYGINLDVENLSYAVLDRDQT 609 Query: 62 PLSARIASAFYPPHFKVAQ--LVTAKQADAGLDAGDYTFVLDIPADFQRDVLAGRAPAVQ 119 LS A + + + + ++ D + +GD + ++IP F RD+ G V Sbjct: 610 GLSQNYALNLSGSRYFIEKPPISDYQELDRRMRSGDISLAIEIPPGFARDIARGHTVEVG 669 Query: 120 LNVDATRMSQAFTGNSYIQQM----VSNEVTQFVGRTEVTGSTPVGLALRMRFNPNLEQS 175 VD ++A T Y+Q M +++ T +G++ + P + R R+NP+++ Sbjct: 670 AWVDGAMPTRAETVRGYVQGMHQLWLADMATHRLGQSL---AMPARIETRYRYNPDVK-- 724 Query: 176 WFGSLMEIINNVT-----MLSIILTGAALIREREHGTIEHLLVMPVSPAEIMLAKVWSMG 230 SL ++ + M+ +LT +++RE+E G+I +L V PVS E +L K Sbjct: 725 ---SLPAMVPAIIPLLLMMIPAMLTALSVVREKELGSILNLYVTPVSRTEFLLGKQLPY- 780 Query: 231 AVVALAALASLLLIVRGALQVPIEGSIALFMLATVLHLFATTSMGIFMATLARSMPQFGM 290 ++ + A L L+ VP++GS+ LA ++ + +T G+ +T S Sbjct: 781 LLLGMLNFAMLTLLAVTVFGVPVKGSLLTLTLAALVFVICSTGFGLLASTFTNSQIAALF 840 Query: 291 LTVL-VLLPLQLLSGGSTPRESMPVPVQDIMLAAPTTHFVAAGQAILYRGAGLEVV---- 345 +T++ ++P +G P S+ I P THF+ + + + L+ + Sbjct: 841 VTMIGTIIPCVQFAGLLNPVSSLEGMGALIGQVYPATHFLTISRGVFSKALALDDLASSF 900 Query: 346 WPQLLA---IFGIGLVLF 360 WP ++A I G+ ++L Sbjct: 901 WPLVVAAPVILGLSVLLL 918 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.136 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,054,172 Number of extensions: 39670 Number of successful extensions: 184 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 178 Number of HSP's successfully gapped: 4 Length of query: 375 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 288 Effective length of database: 1,207,925 Effective search space: 347882400 Effective search space used: 347882400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)