BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1680.p (471 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 659 0.0 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 404 e-113 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 371 e-104 gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 364 e-101 gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipo... 259 2e-70 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 659 bits (1700), Expect = 0.0, Method: Compositional matrix adjust. Identities = 363/452 (80%), Positives = 396/452 (87%), Gaps = 7/452 (1%) Query: 14 ACQSMAPTYQRPAAPVAAHYPQQPAG-------MEAEAQALGWQAYFRDPQLQALITQAL 66 ACQS+AP YQRPAAPVAA YP P EA + L W+ YF DP LQALITQAL Sbjct: 24 ACQSLAPHYQRPAAPVAAVYPDGPVNEAAHQVANEAASPPLPWRDYFTDPPLQALITQAL 83 Query: 67 ANNRDLRVAALRVEEARALYGIQRADLLPSIGAQVGVDRSRTPADLNLTQRPLVGSAYQA 126 N+RDLR AALRV+EA+ALYGI ADL P+IGAQ G+DRSRTPADLNLT+RPLVGSAYQA Sbjct: 84 ENSRDLRTAALRVQEAQALYGIGHADLFPTIGAQAGLDRSRTPADLNLTRRPLVGSAYQA 143 Query: 127 GLGLSNWEIDLWGRLRSLSDAALQSALASEATRRAAALSLVAQVADAYLELAELDERLAI 186 GLGLS+WEIDLWGRLRSL DAAL+SA AS+ATR A ALSL+AQVADA+LELAELDERL I Sbjct: 144 GLGLSSWEIDLWGRLRSLDDAALESARASQATRSAVALSLMAQVADAWLELAELDERLEI 203 Query: 187 ARDTIASRQESYRIFSRRVEVGATSRLNLTQIETLLTQAQALGAQLEQLRAQRVNALSLL 246 A TIASRQES+RIFSRRVEVGATSRLNLTQIETLLTQAQALGAQL+Q RAQR+NAL+LL Sbjct: 204 ANQTIASRQESFRIFSRRVEVGATSRLNLTQIETLLTQAQALGAQLQQQRAQRLNALTLL 263 Query: 247 VGAPLELPVGQARTALNATFPELAAGTPSLLLTRRPDVVAAELRLKAANANIGAARAAFF 306 VG+P+ LPVGQAR L+A F L G P+LLLTRRPDV AAE RL AANANIGAARAAFF Sbjct: 264 VGSPVVLPVGQARAGLDAVFAPLQPGLPALLLTRRPDVAAAEHRLMAANANIGAARAAFF 323 Query: 307 PSISLTGNLGTASAELSGLFRDGSQAWTFSPSITLPIFTAGRLRNNLDLAEVRRDIAVAN 366 PSISLTGN+GTASAELSGLFRDGS AWTFSPSITLPIFTAGRLRNNL L+EVRRD+AVA Sbjct: 324 PSISLTGNVGTASAELSGLFRDGSHAWTFSPSITLPIFTAGRLRNNLALSEVRRDLAVAA 383 Query: 367 YEKTVQGAFRDVADALAARQWLTRQLAIAETAEQVQSERARLSTLRYDNGAAPFLDVLDA 426 YEKTVQ AFR+VADALAARQWLT+QL IA++A +VQ ERARLS LRYDNGAAPFLDVLDA Sbjct: 384 YEKTVQTAFREVADALAARQWLTQQLEIAQSAVRVQRERARLSALRYDNGAAPFLDVLDA 443 Query: 427 QRDLLSARQQRVQIRRALLSSAVALYAALGGD 458 QRDLLSA+QQ VQIRRALLSS+VALYAALGG+ Sbjct: 444 QRDLLSAQQQLVQIRRALLSSSVALYAALGGE 475 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 404 bits (1037), Expect = e-113, Method: Compositional matrix adjust. Identities = 230/455 (50%), Positives = 303/455 (66%), Gaps = 4/455 (0%) Query: 11 LLAACQSMAPTYQRPAAPVAAHYPQQPAG-MEAEAQALGWQAYFRDPQLQALITQALANN 69 +L C S+APTY+RP APV + YP AG E +A LGW+ +F D +LQ LI AL NN Sbjct: 24 VLGGC-SLAPTYERPQAPVESSYPADAAGRAELQAVNLGWRQFFPDQRLQQLIAAALENN 82 Query: 70 RDLRVAALRVEEARALYGIQRADLLPSIGAQVGVDRSRTPADLNLTQRPLVGSAYQAGLG 129 RDLR A L +EEARA Y I RADLLP++ R+RT A +LT +P + ++YQ GL Sbjct: 83 RDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAARTRTAASQSLTGQPTISNSYQVGLA 142 Query: 130 LSNWEIDLWGRLRSLSDAALQSALASEATRRAAALSLVAQVADAYLELAELDERLAIARD 189 + +E+D +GR+R+L DAAL S L++E +++A +SLVAQVA AYL E+L +A + Sbjct: 143 VPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLVAQVAQAYLAERSYAEQLKLADE 202 Query: 190 TIASRQESYRIFSRRVEVGATSRLNLTQIETLLTQAQALGAQLEQLRAQRVNALSLLVGA 249 T+ SRQ + + +R++VGA++ L+ +TL+ A+ QLE+ RAQ NAL+LLVG Sbjct: 203 TLKSRQSNLDLAQKRLDVGASTALDFRLTQTLVESARVAKTQLERQRAQAENALTLLVGK 262 Query: 250 PL-ELPVGQARTALNATFPELAAGTPSLLLTRRPDVVAAELRLKAANANIGAARAAFFPS 308 + ELP Q + ++ AG PS LLT RPD+ +AE L AANANIGAARAAFFP Sbjct: 263 KIGELPPAQLLSD-EKIVTDIPAGLPSDLLTNRPDIRSAEQSLLAANANIGAARAAFFPR 321 Query: 309 ISLTGNLGTASAELSGLFRDGSQAWTFSPSITLPIFTAGRLRNNLDLAEVRRDIAVANYE 368 ISLTGN G+AS+ L GLF GS AW+F PS++LPIF GR NLDLAEVR++ AV YE Sbjct: 322 ISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLPIFDFGRNSANLDLAEVRKNKAVVTYE 381 Query: 369 KTVQGAFRDVADALAARQWLTRQLAIAETAEQVQSERARLSTLRYDNGAAPFLDVLDAQR 428 K +Q AFR+VADAL AR L Q+ E Q ER +L+ LR+ NG A LD LDAQR Sbjct: 382 KAIQTAFREVADALVARGSLDEQVKAQEAFLAAQQERLKLTDLRFKNGIASSLDQLDAQR 441 Query: 429 DLLSARQQRVQIRRALLSSAVALYAALGGDSSQAT 463 DL SA+Q +Q R+ L++A+ LY ALGG ++ T Sbjct: 442 DLFSAQQALIQARQLRLNNAIDLYKALGGGLNENT 476 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 371 bits (952), Expect = e-104, Method: Compositional matrix adjust. Identities = 226/479 (47%), Positives = 293/479 (61%), Gaps = 20/479 (4%) Query: 3 LLLAALPLLLAACQSMAPTYQRPAAPVAAHYPQQPAGMEAEAQA------LGWQAYFRDP 56 +L L +LL C M P Y RP APVAAH+ + A A + +GW+ F DP Sbjct: 15 MLPVMLAMLLGGCTLM-PDYHRPDAPVAAHFAGADSKNAAGASSAPVVADIGWRDVFTDP 73 Query: 57 QLQALITQALANNRDLRVAALRVEEARALYGIQRADLLPSIGAQVGVDRSRTPADLNLTQ 116 LQ +I AL NNRDLRVA L +E+ARA YG+QRA L PSI A SRTP DL+ T Sbjct: 74 SLQQVIALALENNRDLRVAVLNIEKARAQYGVQRAALFPSIKAATSETASRTPGDLSSTG 133 Query: 117 RPLVGSAYQAGLGLSNWEIDLWGRLRSLSDAALQSALASEATRRAAALSLVAQVADAYLE 176 L +Y A LG S +E+DL+GR+RSL + ALQS L++ RR+ +SL+A+VA A+L Sbjct: 134 SALTSRSYTATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQISLIAEVATAWLT 193 Query: 177 LAELDERLAIARDTIASRQESYRIFSRRVEVGATSRLNL----TQIETLLTQAQALGAQL 232 LA +RL +A+DT+ S+ SY + R E+G++S L L T +E+ ++ AQ+ Sbjct: 194 LASDQDRLRLAQDTLESQSSSYALTQRSFELGSSSALTLRQAQTSVESARVDVESYTAQV 253 Query: 233 EQLRAQRVNALSLLVGAPLE---LPVGQARTALNATFP--ELAAGTPSLLLTRRPDVVAA 287 +Q R NAL LLVG L LP G T L A P + G PS LL RR D+V A Sbjct: 254 DQDR----NALVLLVGKDLPTALLPQGLPDTRLAAASPLATIPPGLPSDLLQRRADIVQA 309 Query: 288 ELRLKAANANIGAARAAFFPSISLTGNLGTASAELSGLFRDGSQAWTFSPSITLPIFTAG 347 E L+AANANIGAARAAF+PSI+LT N GTASA L+GLF+ GS +W+F P I+LPIF G Sbjct: 310 ERDLRAANANIGAARAAFYPSITLTVNAGTASARLAGLFKGGSGSWSFVPQISLPIFDGG 369 Query: 348 RLRNNLDLAEVRRDIAVANYEKTVQGAFRDVADALAARQWLTRQLAIAETAEQVQSERAR 407 + NLD+A V RDI+VA YEK +Q AFR+V+DALA R L RQL E Q E R Sbjct: 370 ANQANLDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLQAQEALVQASDEAFR 429 Query: 408 LSTLRYDNGAAPFLDVLDAQRDLLSARQQRVQIRRALLSSAVALYAALGGDSSQATAFP 466 LS RY +G +L VLD+QR L SA++ + + + ++ V Y LGG + T P Sbjct: 430 LSQARYHSGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNLVTFYKTLGGGWVERTGTP 488 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 364 bits (934), Expect = e-101, Method: Compositional matrix adjust. Identities = 223/470 (47%), Positives = 288/470 (61%), Gaps = 16/470 (3%) Query: 15 CQSMAPTYQRPAAPVAAHYPQQ-----------PAGMEAEAQALGWQAYFRDPQLQALIT 63 C ++AP Y +PAAPV A + Q PA E +A LGW+ +F DP+LQ +I Sbjct: 28 CANLAPDYTQPAAPVPASWTSQAAAQPAVGSAGPAAGEIDADHLGWREFFLDPRLQQVIA 87 Query: 64 QALANNRDLRVAALRVEEARALYGIQRADLLPSIGAQVGVDRSRTPADLNLTQRPLVGSA 123 AL NNRDLRVAAL +E+ARA Y I A+ PSI A SRTPA+L+ T V Sbjct: 88 AALDNNRDLRVAALNIEKARAQYRITDANRYPSISATGTSSASRTPAELSTTGSAAVSHQ 147 Query: 124 YQAGLGLSNWEIDLWGRLRSLSDAALQSALASEATRRAAALSLVAQVADAYLELAELDER 183 +G+S +E+D +GRL++LSDAAL+S TRR+ +SLVA+VA A+L LA + Sbjct: 148 QSVTVGVSAYELDFFGRLKNLSDAALESYQYQVETRRSTHISLVAEVATAWLTLASDRDL 207 Query: 184 LAIARDTIASRQESYRIFSRRVEVGATSRLNLTQIETLLTQAQALGAQLEQLRAQRVNAL 243 L +A+DT AS+ ++ + R ++G S ++L E + A+A A+ Q +NAL Sbjct: 208 LKLAQDTYASQSRTFALSQRSHDIGTVSGVDLASQEATVESARADVARYASQVEQDLNAL 267 Query: 244 SLLVGAPLE---LPVGQARTALNATFPELAAGTPSLLLTRRPDVVAAELRLKAANANIGA 300 LLVGA ++ LP G TA + P AG PS +L RRPDV+AAE LK+ANA+IGA Sbjct: 268 RLLVGADIDAALLPAGLPDTA--SVLPATPAGLPSQVLLRRPDVLAAEHTLKSANADIGA 325 Query: 301 ARAAFFPSISLTGNLGTASAELSGLFRDGSQAWTFSPSITLPIFTAGRLRNNLDLAEVRR 360 ARAAFFPSISLT N GTAS L GLF+ GS +WTFSPSI LPIF AG LR +LD A++ R Sbjct: 326 ARAAFFPSISLTANGGTASRTLGGLFKAGSGSWTFSPSINLPIFNAGSLRASLDSAKITR 385 Query: 361 DIAVANYEKTVQGAFRDVADALAARQWLTRQLAIAETAEQVQSERARLSTLRYDNGAAPF 420 DI VANYEK +Q AFR+VADALA R L ++L A + RLS RY NG + Sbjct: 386 DIDVANYEKAIQTAFREVADALAVRATLDQRLDAQGKATAASEKAFRLSDARYRNGVDSY 445 Query: 421 LDVLDAQRDLLSARQQRVQIRRALLSSAVALYAALGGDSSQATAFPLPSG 470 L L AQR L SA+Q + +R S+ + LY LGG S+ T P G Sbjct: 446 LTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVLGGGWSEQTQPMQPGG 495 >gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 481 Score = 259 bits (663), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 184/459 (40%), Positives = 257/459 (55%), Gaps = 8/459 (1%) Query: 8 LPLL-LAACQSMAPTYQRPAAPVAAHYPQQ-----PAGMEAEAQALGWQAYFRDPQLQAL 61 LP L L AC + A + HYP+ PA A A+GWQ YF LQAL Sbjct: 12 LPTLALCACAPLTAPLPPSALDLPTHYPRTLAEDAPANAAPTASAIGWQDYFPQAPLQAL 71 Query: 62 ITQALANNRDLRVAALRVEEARALYGIQRADLLPSIGAQVGVDRSRTPADLNLTQRPLVG 121 I +AL +N DLR LR+++ +A + IQR+ LP++ + RSR P + + Sbjct: 72 IAEALRHNPDLRDIRLRLQQVQAQWRIQRSTGLPTVNLEAQARRSRGPLEPPESGPAAST 131 Query: 122 SAYQAGLGLSNWEIDLWGRLRSLSDAALQSALASEATRRAAALSLVAQVADAYLELAELD 181 Y+ GLG S+WE+D WGR+ SL++AALQ +AS A +A +SL+ QVA A+ L ELD Sbjct: 132 REYRVGLGASSWELDFWGRIASLNEAALQEWMASRAAEQALVISLIGQVAAAWTSLRELD 191 Query: 182 ERLAIARDTIASRQESYRIFSRRVEVGATSRLNLTQIETLLTQAQALGAQLEQLRAQRVN 241 ERL + A+ ES + R + G L Q + L Q +A A+L+Q+RA + + Sbjct: 192 ERLHLLETLQAATAESLALMQARHDAGRADAAALAQQRSELAQMRAEQAELQQMRASQRS 251 Query: 242 ALSLLVGAPLELPVGQARTALNATFP-ELAAGTPSLLLTRRPDVVAAELRLKAANANIGA 300 L +L G +LP P LA G PS LL RPD++AAE RL+A+ A + A Sbjct: 252 TLEMLAGL-TQLPAALEPAPTTILLPFALAPGLPSTLLAARPDIIAAEHRLQASAAQVAA 310 Query: 301 ARAAFFPSISLTGNLGTASAELSGLFRDGSQAWTFSPSITLPIFTAGRLRNNLDLAEVRR 360 ARAAFFP ISLT G AS L LF+ S+AW F P I+LP+ G ++ D+A + + Sbjct: 311 ARAAFFPRISLTAFGGVASTALQDLFKGPSRAWNFVPEISLPLLDGGLRQSRYDIAALDQ 370 Query: 361 DIAVANYEKTVQGAFRDVADALAARQWLTRQLAIAETAEQVQSERARLSTLRYDNGAAPF 420 A+ +YE+TVQ AFR+VA L WL QL + Q Q++R +R G A Sbjct: 371 QRALLDYERTVQNAFREVAQRLEEAHWLKEQLRLRREVVQQQAQRQAQMHMRLRYGRASR 430 Query: 421 LDVLDAQRDLLSARQQRVQIRRALLSSAVALYAALGGDS 459 LDVL+++RD L A+Q++ + ALL + +ALY ALGG + Sbjct: 431 LDVLESERDHLRAQQRQHMLEHALLRNQIALYVALGGGT 469 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.130 0.358 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,154,380 Number of extensions: 40910 Number of successful extensions: 268 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 218 Number of HSP's successfully gapped: 18 Length of query: 471 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 382 Effective length of database: 1,198,455 Effective search space: 457809810 Effective search space used: 457809810 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)