BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1679.p (122 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311151|ref|YP_003775243.1| histidine-containing phosphotra... 204 1e-54 >gi|300311151|ref|YP_003775243.1| histidine-containing phosphotransfer (HPt) domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 122 Score = 204 bits (519), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 98/122 (80%), Positives = 110/122 (90%) Query: 1 VNDHPYQHINPEPLLDAMGGDLAACRHMFGTFAQSAPATFARLEQAIRADDAPQVKREAH 60 +NDH YQHINPEPLL+AMGGD ACRHMFGTFA+SAPA FARLE AI ADDA QVKREAH Sbjct: 1 MNDHHYQHINPEPLLEAMGGDATACRHMFGTFAKSAPANFARLEAAILADDAAQVKREAH 60 Query: 61 SLKSMTALVGAGELTDLLKSVELQSRAEPPQSSQPRLAQLQALFSDVAAEVRHFVDQVGV 120 SLKSMTALVGAG LT+LLKS+E+QSRAEP QS+QPRLA+LQ+LF+ AAEV+H+VDQVGV Sbjct: 61 SLKSMTALVGAGVLTELLKSIEVQSRAEPAQSAQPRLAELQSLFAATAAEVQHYVDQVGV 120 Query: 121 AQ 122 A Sbjct: 121 AH 122 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.130 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 402,996 Number of extensions: 14883 Number of successful extensions: 55 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 55 Number of HSP's successfully gapped: 1 Length of query: 122 Length of database: 1,619,870 Length adjustment: 74 Effective length of query: 48 Effective length of database: 1,269,480 Effective search space: 60935040 Effective search space used: 60935040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 125 (52.8 bits)