BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1667.p (343 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspi... 532 e-152 gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspi... 129 4e-31 gi|300309398|ref|YP_003773490.1| threonine dehydratase [Herbaspi... 121 1e-28 gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspi... 115 6e-27 >gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 351 Score = 532 bits (1371), Expect = e-152, Method: Compositional matrix adjust. Identities = 287/341 (84%), Positives = 315/341 (92%) Query: 1 LLPSLGEIEQAAGIVYAGMASTPQLCWPLLNQALGAEVWVKHENHAPTGAFKVRGGMVYL 60 LLPSL +IEQAAGIVYAGMA T QLCWPLLNQALGAEVWVKHENHAPTGAFKVRGGMVYL Sbjct: 8 LLPSLTDIEQAAGIVYAGMAPTAQLCWPLLNQALGAEVWVKHENHAPTGAFKVRGGMVYL 67 Query: 61 HHLARQQPQLSGVISATRGNHGQSVGLSARRFGIAATIVVPEGNSREKNVAMRALGVDLI 120 HHLARQQPQLSGV+SATRGNHGQSVGL+ARR GI+ATIVVP+GNSREKN AMRALGV+LI Sbjct: 68 HHLARQQPQLSGVVSATRGNHGQSVGLAARRLGISATIVVPQGNSREKNAAMRALGVELI 127 Query: 121 EHGREFQDSREHAQQLAAERQWHMIPSLHRNLVAGVATYWMELFTSQPALDLVLVPVGQG 180 EHG EFQ+SRE+AQQLAA++Q HMIPSLHR+LVAGVA+YWMELF +QP LD+VLVP+GQG Sbjct: 128 EHGSEFQESREYAQQLAAQQQLHMIPSLHRDLVAGVASYWMELFAAQPGLDVVLVPIGQG 187 Query: 181 SGICGAVAARNALGLKTRIIGVVSAHALAYKLSFDAGRKIEAPVTTHLADGVACRVPDQA 240 SG+CGAVAARNALGL TRIIGVVSAHALAYKLSF+AGRKIE+PV+T +ADGVACRVPDQA Sbjct: 188 SGMCGAVAARNALGLSTRIIGVVSAHALAYKLSFEAGRKIESPVSTCIADGVACRVPDQA 247 Query: 241 ALEVMFAHVDEVVAVTDDEVMDAMKLAFIATHNVVEGAGACALAAALQLRTRLRGKKIGV 300 +LEV+F+ VDEVVAVTDDEVMDAMKLA+IATHNV EGAGACALAAA QLR RLRG K+GV Sbjct: 248 SLEVLFSEVDEVVAVTDDEVMDAMKLAYIATHNVAEGAGACALAAAWQLRARLRGLKVGV 307 Query: 301 TFSGGNVDHDVYAQVLARPSALADALLAPAQVHALAATPGR 341 T SGGNVDHDV A+VLAR +A AD LLAPAQV L + P R Sbjct: 308 TLSGGNVDHDVLARVLARAAAPADMLLAPAQVLPLTSLPQR 348 >gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 129 bits (323), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 105/317 (33%), Positives = 155/317 (48%), Gaps = 2/317 (0%) Query: 2 LPSLGEIEQAAGIVYAGMASTPQLCWPLLNQALGAEVWVKHENHAPTGAFKVRGGMVYLH 61 LPS ++ AA + TP L + LGA+++ K EN GAFK RG M L Sbjct: 10 LPSFADVLLAAERIKPWAHRTPVLRSRTADTELGAQLFFKCENFQRMGAFKFRGAMNALT 69 Query: 62 HLARQQPQLSGVISATRGNHGQSVGLSARRFGIAATIVVPEGNSREKNVAMRALGVDLIE 121 Q + GVI+ + GNH Q L+A+ GI A IV+P+ K A R G +++ Sbjct: 70 QFDATQRK-HGVITFSSGNHAQGTALAAQLLGIPAVIVMPQDAPAAKVAATRGYGAEVVT 128 Query: 122 HGREFQDSREHAQQLAAERQWHMIPSL-HRNLVAGVATYWMELFTSQPALDLVLVPVGQG 180 + R +D +LAAER+ +IP H +++AG T +EL LD + V +G G Sbjct: 129 YDRYTEDREAIGARLAAERKMTLIPPYDHPHVMAGQGTATLELLQETGPLDALFVCLGGG 188 Query: 181 SGICGAVAARNALGLKTRIIGVVSAHALAYKLSFDAGRKIEAPVTTHLADGVACRVPDQA 240 + GA A AL +I GV + S AG+ + +ADG + + Sbjct: 189 GLLSGAALAARALSPACKIYGVEPQAGNDGQQSLRAGQVVHIDTPKTIADGAQTQHLGKY 248 Query: 241 ALEVMFAHVDEVVAVTDDEVMDAMKLAFIATHNVVEGAGACALAAALQLRTRLRGKKIGV 300 V+ VD++ V+D+E++ M+ VVE G AAA +L+ +L+GK++GV Sbjct: 249 TFGVIRELVDDIHTVSDEELVAGMRFFAERMKMVVEPTGCLGFAAARKLKDQLQGKRVGV 308 Query: 301 TFSGGNVDHDVYAQVLA 317 SGGNVD A LA Sbjct: 309 IISGGNVDLGKLATFLA 325 >gi|300309398|ref|YP_003773490.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 401 Score = 121 bits (303), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 96/319 (30%), Positives = 162/319 (50%), Gaps = 5/319 (1%) Query: 2 LPSLGEIEQAAGIVYAGMASTPQLCWPLLNQALGAEVWVKHENHAPTGAFKVRGGMVYLH 61 + +L +IE AA + + TP + L+Q +GA+V++K EN T +FK RG L Sbjct: 1 MTTLNDIEAAARRLQGQILETPCVESRTLSQIVGAQVFLKFENLQFTASFKERGACNKLV 60 Query: 62 HLARQQPQLSGVISATRGNHGQSVGLSARRFGIAATIVVPEGNSREKNVAMRALGVDLIE 121 +A GV++ + GNH Q V A+R G+ ATIV+P K R G +++ Sbjct: 61 DVAASGGGARGVVAMSAGNHAQGVAYHAQRLGLPATIVMPRFTPGVKIERTRGFGANVVL 120 Query: 122 HGREFQDSREHAQQLAAERQWHMI-PSLHRNLVAGVATYWMELFTSQPALDLVLVPVGQG 180 HG + +R HA +LA + + P ++AG T +E+ + P LD ++V +G G Sbjct: 121 HGDTLEAARTHAYELAEKEGLTFVHPYDDEAIIAGQGTVALEMMKAVPDLDCLVVSIGGG 180 Query: 181 SGICGAVAARNALGLKTRIIGVVSAHALAYKLSFDAGRKIEAPV-TTHLADGVACRVPDQ 239 + G A A+ + I+GV + + +A + E PV + +A+G+A P Sbjct: 181 GLLAGMAVAARAIKPEIEIVGVQTRR---FPTMVNAVKGTEHPVGASTIAEGIAVGTPGT 237 Query: 240 AALEVMFAHVDEVVAVTDDEVMDAMKLAFIATHNVVEGAGACALAAALQLRTRLRGKKIG 299 ++ DE++ V + ++ A+ + +VEGAGA LAA ++ R RGK++G Sbjct: 238 LTRAIIAREADELLLVDEGDIEQAVLMLLEIEKTLVEGAGAAGLAALVRYPDRFRGKRVG 297 Query: 300 VTFSGGNVDHDVYAQVLAR 318 + SGGN+D + ++ R Sbjct: 298 LVLSGGNIDPLLLTAIIER 316 >gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 508 Score = 115 bits (288), Expect = 6e-27, Method: Compositional matrix adjust. Identities = 94/305 (30%), Positives = 145/305 (47%), Gaps = 10/305 (3%) Query: 15 VYAGMASTPQLCWPLLNQALGAEVWVKHENHAPTGAFKVRGGMVYLHHLARQQPQLSGVI 74 VY TP L+Q + ++ K E+ +FK+RG + HL Q + GVI Sbjct: 14 VYDVAQETPLELANTLSQRIDNRIYFKREDMQSVFSFKLRGAYNKMAHLTPAQLK-RGVI 72 Query: 75 SATRGNHGQSVGLSARRFGIAATIVVPEGNSREKNVAMRALGVDLIEHGREFQDSREHAQ 134 A+ GNH Q V LSA R G A IV+P + K A++A G +++ G F D+ EHA Sbjct: 73 CASAGNHAQGVALSAARLGCRAVIVMPTTTPQVKIDAVKARGGEVVLFGDSFTDAYEHAL 132 Query: 135 QLAAERQWHMI-PSLHRNLVAGVATYWMELFTSQP-ALDLVLVPVGQGSGICGAVAARNA 192 L +++ + P ++AG T ME+ P + + V +G G I G + A Sbjct: 133 TLEKKQKLTFVHPFDDPYVIAGQGTVGMEILRQHPDPIHAIFVAIGGGGLIAGVASYVKA 192 Query: 193 LGLKTRIIGVVSAHALAYKLSFDAGRKIEAPVTTHLADGVACRVPDQAALEVMFAHVDEV 252 + +IIGV + + A S AGR++ P +DG A ++ + + VDEV Sbjct: 193 VRPDIKIIGVQTTDSDAMARSLKAGRRVALPDVGLFSDGTAVKLVGEETFRIAKELVDEV 252 Query: 253 VAVTDDEVMDAMKLAFIATHNVVEGAGACALAAALQLRTR-------LRGKKIGVTFSGG 305 + V D V A+K F T ++VE AGA A+A A R ++G+ + G Sbjct: 253 IIVDTDAVCTAIKDIFQDTRSIVEPAGALAVAGAKAYVERAKASKKPIKGESLITIACGA 312 Query: 306 NVDHD 310 N++ D Sbjct: 313 NMNFD 317 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,012,874 Number of extensions: 43106 Number of successful extensions: 142 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 137 Number of HSP's successfully gapped: 4 Length of query: 343 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 257 Effective length of database: 1,212,660 Effective search space: 311653620 Effective search space used: 311653620 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)