BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1662.p (457 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD ... 787 0.0 gi|300312349|ref|YP_003776441.1| hemolysin-type secretion transm... 182 7e-47 gi|300312188|ref|YP_003776280.1| hemolysin secretion-related tra... 105 9e-24 >gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD family secretion protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 787 bits (2033), Expect = 0.0, Method: Compositional matrix adjust. Identities = 405/457 (88%), Positives = 429/457 (93%) Query: 1 MTLSLKPGTGHGIASRSKGFMRRVAPRLDRRLGELQAMPRPRLAVLTCAALLVLLVVWSI 60 MTLSLKP GI +R +GFMRR+APRLDRRLGEL MPRPRLAVL LL LLV+WS+ Sbjct: 1 MTLSLKPNATPGIGARGQGFMRRLAPRLDRRLGELATMPRPRLAVLAVTVLLALLVLWSV 60 Query: 61 FAPIDMVVRGSGRIVASEHNQVIQHLEGGIVSAILVREGAVVKKGQTLISIADVRANADL 120 FAPIDMVVRGSGRIVASEHNQVIQHLEGGIVSAILVREGA VKKGQTLISI+DVRANADL Sbjct: 61 FAPIDMVVRGSGRIVASEHNQVIQHLEGGIVSAILVREGAAVKKGQTLISISDVRANADL 120 Query: 121 GEGRVKILGLRAKIARLSAEASGAASLQMPADLDHDDPAVQSEQAAFAARSAKLMQELSV 180 GEGRVKILGLRAKIARLSAEASGA+SLQMP DL+ DPAVQSEQAAFAARS+KLMQE+SV Sbjct: 121 GEGRVKILGLRAKIARLSAEASGASSLQMPDDLERGDPAVQSEQAAFAARSSKLMQEMSV 180 Query: 181 LREQAAQRQAELGEAISRQKSQASELDLAQRQYKLIHNMLERSAASQLEDIQAAARVQDA 240 LREQ+AQRQAELGEAISRQKSQASEL+LAQRQYKLIH+ML R+AASQLEDIQAA+RVQDA Sbjct: 181 LREQSAQRQAELGEAISRQKSQASELELAQRQYKLIHDMLVRNAASQLEDIQAASRVQDA 240 Query: 241 QSKLAANDAMIPRLKAAIAESQAKMGEAASRFRSDARTELTAAETELARLQEEIKSRNDR 300 QSKLAA +AMIPRL AAIAE+QAK GEAASRFRSDARTELTAAETELARLQEEIKSRNDR Sbjct: 241 QSKLAATNAMIPRLNAAIAEAQAKQGEAASRFRSDARTELTAAETELARLQEEIKSRNDR 300 Query: 301 VSRSEVKAPMDGVVNRVMINTVGGVVRPGDPIIEMTPSDDKLVIEARISPTDRAELVPGA 360 VSRSEVKAPMDGVVNRVMINT+GGVVRPGDPIIEMTPSDDKLVIEARISPTDRAELV GA Sbjct: 301 VSRSEVKAPMDGVVNRVMINTIGGVVRPGDPIIEMTPSDDKLVIEARISPTDRAELVTGA 360 Query: 361 RARVKVSAYDYGVYGAMEGVVTEISADTVPDDNNPRGERSYRIKIAVQRGDHALQRKLAL 420 RARVKVSAYDYGVYGAM+GVVTEISADTVPD+ +PRGER YR+KI V+RG LQ+KLAL Sbjct: 361 RARVKVSAYDYGVYGAMDGVVTEISADTVPDEGSPRGERFYRLKIEVKRGHDELQQKLAL 420 Query: 421 MPGMTASADIIVGRRTVWQYLMSPLSRFGQGALREPR 457 MPGMTASADIIVGRRTVWQYLMSPLSRFGQGALREPR Sbjct: 421 MPGMTASADIIVGRRTVWQYLMSPLSRFGQGALREPR 457 >gi|300312349|ref|YP_003776441.1| hemolysin-type secretion transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 386 Score = 182 bits (461), Expect = 7e-47, Method: Compositional matrix adjust. Identities = 126/416 (30%), Positives = 215/416 (51%), Gaps = 55/416 (13%) Query: 46 LTCAALLVLLVVWSIFAPIDMVVRGSGRIVASEHNQVIQHLEGGIVSAILVREGAVVKKG 105 L+ A++LVLL+ W+ + ID V R G+++A Q +Q +GG V ILV+EG VK+G Sbjct: 19 LSMASVLVLLL-WAAVSQIDQVTRAQGQVIAVSRTQSVQAPDGGPVKQILVKEGEAVKQG 77 Query: 106 QTLISIADVRANADLGEGRVKILGLRAKIARLSAEASGAASLQMPADLDHDDPAVQSEQA 165 Q L+ + R L + R K+ LR +ARL AE G P LD+ + + +++ Sbjct: 78 QLLMVLEQDRVQTSLNDSRAKVAALRISLARLRAEVYGHPLNFEPDLLDYKE-YIANQKD 136 Query: 166 AFAARSAKLMQELSVLREQAAQRQAELGEAISRQKSQASELDLAQRQYKLIHNMLERSAA 225 + R + Q+L+ L++ L LA ++ ++ + LER+ Sbjct: 137 LYVKRKILIDQDLASLQDM---------------------LGLANQELEM-NKSLERTG- 173 Query: 226 SQLEDIQAAARVQDAQSKLAANDAMIPRLKAAIAESQAKMGEAASRFRSDARTELTAAET 285 D+ A + RL+ +A+ +A++ +++ +A ++T + Sbjct: 174 ----DVGRAD---------------VLRLQRNVADIRAQINNKRNKYFQEALVDMTKVQE 214 Query: 286 ELARLQEEIKSRNDRVSRSEVKAPMDGVVNRVMINTVGGVVRPGDPIIEMTPSDDKLVIE 345 +L +E+++ R + +E+KAP DGVV + + T+GGVVR GD ++E+ P D+ L++E Sbjct: 215 DLNTQREQLEDRTQVLEHTELKAPADGVVKNIKVTTIGGVVRSGDVVMEILPVDN-LIVE 273 Query: 346 ARISPTDRAELVPGARARVKVSAYDYGVYGAMEGVVTEISADTVPDDNNPRGERSYRIKI 405 A++S D A + G A VK+ AYDY +YG + G V +S DT+ D+ YR+ I Sbjct: 274 AKVSTADSAYVKVGQAANVKLDAYDYSIYGTLNGHVVYVSPDTLLDETRQGAMPYYRVHI 333 Query: 406 AVQ------RGDHALQRKLALMPGMTASADIIVGRRTVWQYLMSPLSRFGQGALRE 455 A++ R A+Q K PGMTA+ +I RTV +L P+++ Q ++ E Sbjct: 334 ALEEAVFKGRKADAIQVK----PGMTATVEIKANTRTVLSFLTKPIAKTLQESMGE 385 >gi|300312188|ref|YP_003776280.1| hemolysin secretion-related transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 447 Score = 105 bits (261), Expect = 9e-24, Method: Compositional matrix adjust. Identities = 122/431 (28%), Positives = 203/431 (47%), Gaps = 44/431 (10%) Query: 35 LQAMPRPRLAVLTCAALLVLLVV----WSIFAPIDMVVRGSGRIVASEHNQVIQHLEGGI 90 LQ P R ++ A LLV +V+ WS+ ID+VV +G+++ S + I ++ Sbjct: 50 LQEAPDSR-SLRWTARLLVGMVIFALLWSLLGRIDIVVSANGKVIPSARTKTIASVDVSA 108 Query: 91 VSAILVREGAVVKKGQTLISIADVRANADLGEGRVKILGLRAKIARLSAEASGAASLQMP 150 V AILV EG +VK G+ LI + ++A+ + + R + AR A +L+ P Sbjct: 109 VRAILVSEGQLVKAGEVLIELDSSSSDAERDKAADAVAQARLQAARAQAMMEAIQNLRTP 168 Query: 151 --ADLDHDDPAVQSEQAAFAARSAKLMQELSVLREQAAQRQAELGEAISRQKSQASELDL 208 A + P V QA + A ++L+ + R R L + ISR + L L Sbjct: 169 ELAQV----PGV--AQAQWEAVRSQLVAQYQDFRA----RLGRLDDEISR---YGAALKL 215 Query: 209 AQRQYKLIHNMLERSAASQ---LEDIQAAARVQDAQSKLAANDAMIPRLKAAIAESQAKM 265 A ++ ++ S+ LE Q ARV D Q +L +DA R + A +Q + Sbjct: 216 ATQRASDYQTLVADHTVSRHAWLEKEQ--ARV-DLQGQL--DDA---RNQRATLVAQTRK 267 Query: 266 GEAASRFRSDARTELTAAETELARLQEEIKSRNDRVSRSEVKAPMDGVVNRVMINTVGGV 325 SR +D E + Q++ + ++ ++ AP+ G V ++ ++T+GGV Sbjct: 268 DAHDSRIEADKTIEAS---------QQDQRRADEHSKLLKLVAPVTGTVQQLTVHTIGGV 318 Query: 326 VRPGDPIIEMTPSDDKLVIEARISPTDRAELVPGARARVKVSAYDYGVYGAMEGVVTEIS 385 V P++++ P D ++ +EA + D + G A VK+ A+DY YG + V IS Sbjct: 319 VPAAQPLMQIVPQDSQIEVEAMLENKDVGFVEIGQAAEVKIDAFDYTKYGTLPARVVHIS 378 Query: 386 ADTVPDDNNPRGERSYRIKIAVQRGDHALQ-RKLALMPGMTASADIIVGRRTVWQYLMSP 444 D + D+ Y +I + + + RK+ L GM A+ +I G R V +Y +SP Sbjct: 379 RDAIQDEKKG---LLYSARIVLDQSSLIVDGRKVQLSAGMAANVEIKTGNRRVIEYALSP 435 Query: 445 LSRFGQGALRE 455 L R + AL E Sbjct: 436 LIRHQKEALHE 446 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.131 0.354 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,266,996 Number of extensions: 48957 Number of successful extensions: 305 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 303 Number of HSP's successfully gapped: 5 Length of query: 457 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 369 Effective length of database: 1,203,190 Effective search space: 443977110 Effective search space used: 443977110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)