BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1655.p (249 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herb... 440 e-125 gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herba... 125 4e-30 gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herb... 121 7e-29 gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogena... 121 7e-29 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 120 1e-28 >gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 440 bits (1131), Expect = e-125, Method: Compositional matrix adjust. Identities = 219/249 (87%), Positives = 236/249 (94%) Query: 1 MTDKVIIVTGGSRGIGAATALLAGQRGYAVCVNYVSNREAAEAVAGQIRAAGGRAITVAG 60 M DKVI+VTGGSRGIGAATALLAGQRGYAV VNYV+NR AA+AV QI+AAGGRAI V G Sbjct: 3 MADKVILVTGGSRGIGAATALLAGQRGYAVAVNYVTNRAAADAVVAQIQAAGGRAIAVGG 62 Query: 61 DVSKEEDVLRIFTTTDQQLGRVTALVNNAGILEHQSRLDAMSAERITRVLVANVTGSLLC 120 DV++EEDVL++F T D +LGRVTALVNNAG+L+HQSRL+ MSAERI RVL+ANVTGSLLC Sbjct: 63 DVAREEDVLQLFATVDGELGRVTALVNNAGVLDHQSRLEDMSAERINRVLIANVTGSLLC 122 Query: 121 AREAVKRMSTRHGGQGGAIVNLSSMAAKLGGPGEYVDYAASKGAIDAFTVGLAKEVAAEG 180 AREAVKRMSTRHGGQGGAIVNLSSMAAKLGGPGEYVDYAASKGAIDAFT+GLAKEVAAEG Sbjct: 123 AREAVKRMSTRHGGQGGAIVNLSSMAAKLGGPGEYVDYAASKGAIDAFTIGLAKEVAAEG 182 Query: 181 IRVNAVRPGLIYTDIHASGGEAGRVDRLKDAVPMKRGGSAEEVAHAILWLLSDESSYATG 240 IRVNAVRPGLIYTDIHASGGEAGRVDRLKDAVPMKRGGSA EVA+AILWLLS+E+SYATG Sbjct: 183 IRVNAVRPGLIYTDIHASGGEAGRVDRLKDAVPMKRGGSAAEVANAILWLLSEEASYATG 242 Query: 241 TFIDVSGGR 249 TFIDVSGGR Sbjct: 243 TFIDVSGGR 251 >gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 253 Score = 125 bits (314), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 93/248 (37%), Positives = 142/248 (57%), Gaps = 6/248 (2%) Query: 4 KVIIVTGGSRGIGAATALLAGQRGYAVCVNYVSNREAAEAVAGQIRAAGGRAITVAGDVS 63 KV+ ++G S GIGAA A RG + V+Y S+ + AEAVA ++RAAG ++IT+ DV Sbjct: 7 KVVFISGASTGIGAAAARAFAARGSHLVVHYNSSEKEAEAVAAEVRAAGSKSITIGADVR 66 Query: 64 KEEDVLRIFTTTDQQLGRVTALVNNAGILEHQSRLDAMSAERITRVLVANVTGSLLCARE 123 + Q+GR+ L+NNAG L ++ L+ ++ E ++ N + R Sbjct: 67 DTSAINAAVAKAVAQMGRIDVLINNAGALVKRAPLETVTDELFDDIININARSVVAFTRA 126 Query: 124 AVKRMSTRHGGQGGAIVNLSSMAAKL-GGPGEYVDYAASKGAIDAFTVGLAKEVAAEGIR 182 + M + GG I+N++S+AA+ GGPG + YAASKG + T G+AKE+ A+ IR Sbjct: 127 VIPVMRAQGGGN---IINVTSVAARHGGGPGALI-YAASKGFVSTLTRGMAKELLADKIR 182 Query: 183 VNAVRPGLIYTDIHASGGEAGRVDRLKDAVPMKRGGSAEEVAHAILWLLSDE-SSYATGT 241 VNAV PG+I T +++ + +PM R G +E + A L+L S++ S Y TG Sbjct: 183 VNAVAPGVIMTPFQERFTTPEQLEGFRKTIPMGRIGEPDECSGAFLYLASEQMSGYVTGQ 242 Query: 242 FIDVSGGR 249 IDV+GG+ Sbjct: 243 IIDVNGGQ 250 >gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 121 bits (303), Expect = 7e-29, Method: Compositional matrix adjust. Identities = 85/249 (34%), Positives = 129/249 (51%), Gaps = 10/249 (4%) Query: 1 MTDKVIIVTGGSRGIGAATALLAGQRGYAVCVNYVSNREAAEAVAGQIRAAGGRAITVAG 60 + KV V GGSRGIGAA G V YVS+ + A+A+ ++A GGRA+ + Sbjct: 15 LVSKVAFVQGGSRGIGAAIVQRLAAEGATVAFTYVSSPDKAQALVSSVQAKGGRALAIQA 74 Query: 61 DVSKEEDVLRIFTTTDQQLGRVTALVNNAGILEHQSRLDAMSAERITRVLVANVTGSLLC 120 D + + + + GR+ LVNNAG+L LD E + R L NV + Sbjct: 75 DSADAAALQQAIRLAAETFGRLDILVNNAGVLAM-GPLDQFKLEDLDRTLAVNVRSVFVA 133 Query: 121 AREAVKRMSTRHGGQGGAIVNL-SSMAAKLGGPGEYVDYAASKGAIDAFTVGLAKEVAAE 179 +EA + M G+GG ++++ S+ A ++ G V YA SK A+ T GLA+++ Sbjct: 134 TQEAARYM-----GEGGRVISIGSTNADRIPFAGGAV-YAMSKSALVGLTKGLARDLGPR 187 Query: 180 GIRVNAVRPGLIYTDIHASGGEAGRVDRLKDAVPMKRGGSAEEVAHAILWLLSDESSYAT 239 GI VN V+PG + TD++ + G+ L + + R G AEE+A + +L ES Y T Sbjct: 188 GITVNNVQPGPVDTDMNPASGDFAA--SLTGLMALPRYGKAEEIASFVAYLAGPESGYIT 245 Query: 240 GTFIDVSGG 248 G + + GG Sbjct: 246 GANLMIDGG 254 >gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 121 bits (303), Expect = 7e-29, Method: Compositional matrix adjust. Identities = 95/251 (37%), Positives = 129/251 (51%), Gaps = 13/251 (5%) Query: 1 MTDKVIIVTGGSRGIGAATALLAGQRGYAVCVNYVSNREAAEAVAGQIRAAGGRAITVAG 60 DKV++VTGGS GIG ATA L G V YVS R A + + +R G A+ V G Sbjct: 7 FEDKVVVVTGGSEGIGLATAKLFAAEGARV---YVSGRRA-DKLEQAVREIGNGAVGVPG 62 Query: 61 DVSKEEDVLRIFTTTDQQLGRVTALVNNAGILEHQSR-LDAMSAERITRVLVANVTGSLL 119 D +K +D+ R++ GR+ L NAG +++ L + E + NV G L Sbjct: 63 DAAKLDDLDRLYARVQADHGRLDVLFANAGTASTEAKPLGMIGEEDFDGLFNLNVRGLLF 122 Query: 120 CAREAVKRMSTRHGGQGGAIVNLSSMAAKLGGPGEYVDYAASKGAIDAFTVGLAKEVAAE 179 ++A+ MS QGGAIV S+A G PG+ + Y ASK A+ +F ++ Sbjct: 123 SVQKALPLMS-----QGGAIVLNGSVAGVKGFPGQSL-YNASKAAVRSFARSWTADLKER 176 Query: 180 GIRVNAVRPGLIYTDIHASGGEA--GRVDRLKDAVPMKRGGSAEEVAHAILWLLSDESSY 237 GIRVN V PG T + EA G D LK VP+ R G +E+ A+L+L S ESSY Sbjct: 177 GIRVNVVAPGGTETRLMRDYLEARPGMEDVLKQVVPLGRLGEPDEIGRAVLFLASAESSY 236 Query: 238 ATGTFIDVSGG 248 G + V GG Sbjct: 237 IAGVELFVDGG 247 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 120 bits (301), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 87/245 (35%), Positives = 128/245 (52%), Gaps = 6/245 (2%) Query: 4 KVIIVTGGSRGIGAATALLAGQRGYAVCVNYVSNREAAEAVAGQIRAAGGRAITVAGDVS 63 +V +VTGG+ GIGA+ + +G+ V V + N +AA A + AAG RA + D+ Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADI-NLDAASKTADALVAAGHRAAPLQMDLG 67 Query: 64 KEEDVLRIFTTTDQQLGRVTALVNNAGILEHQSRLDAMSAERITRVLVANVTGSLLCARE 123 E + F ++ GR LVNNAG+ S LD E + + NVTG LL + Sbjct: 68 SPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLD-YPLENWLQTMNVNVTGVLLAGQH 126 Query: 124 AVKRMSTRHGGQGGAIVNLSSMAAKLGGPGEYVDYAASKGAIDAFTVGLAKEVAAEGIRV 183 A + M + Q G IVN+SS++ G G Y SK A+ T +A E+A GI V Sbjct: 127 AARLMVKQ---QWGRIVNISSISGIRAGAGR-TAYGTSKAAVIGLTRQMAIELAQYGITV 182 Query: 184 NAVRPGLIYTDIHASGGEAGRVDRLKDAVPMKRGGSAEEVAHAILWLLSDESSYATGTFI 243 N+V PG + T + + + VPM+R G+ +E+A A+ +L ++ES+Y TG I Sbjct: 183 NSVAPGPVDTPMTQAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESAYITGHVI 242 Query: 244 DVSGG 248 V GG Sbjct: 243 PVDGG 247 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.132 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 758,457 Number of extensions: 29503 Number of successful extensions: 259 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 165 Number of HSP's successfully gapped: 45 Length of query: 249 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 166 Effective length of database: 1,226,865 Effective search space: 203659590 Effective search space used: 203659590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)