BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1632.p (772 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311101|ref|YP_003775193.1| NADH dehydrogenase I subunit G ... 1393 0.0 gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydroge... 221 2e-58 >gi|300311101|ref|YP_003775193.1| NADH dehydrogenase I subunit G [Herbaspirillum seropedicae SmR1] Length = 772 Score = 1393 bits (3606), Expect = 0.0, Method: Compositional matrix adjust. Identities = 728/772 (94%), Positives = 750/772 (97%) Query: 1 MVEIEIDGKKVEVQEGSMVMDAANKIGTYIPHFCYHKKLSIAANCRMCLVEVEKAPKPLP 60 MVEIEIDGKKVEVQEGSMVMDAANK+GTYIPHFCYHKKLSIAANCRMCLVEVEKAPKPLP Sbjct: 1 MVEIEIDGKKVEVQEGSMVMDAANKLGTYIPHFCYHKKLSIAANCRMCLVEVEKAPKPLP 60 Query: 61 ACATPVNNGMIVRTASDKAVKAQKGVMEFLLINHPLDCPICDQGGECQLQDLAVGYGGST 120 ACATPVN GMIVRTASDKAVKAQKGVMEFLLINHPLDCPICDQGGECQLQDLAVGYGGS+ Sbjct: 61 ACATPVNAGMIVRTASDKAVKAQKGVMEFLLINHPLDCPICDQGGECQLQDLAVGYGGSS 120 Query: 121 SRYEEEKRVVEPKDVGPLISMKEMNRCIHCTRCVRFGQEVAGVMELGMLGRGEHSEITTF 180 SRY+EEKRVVEPKDVGPLISMKEMNRCIHCTRCVRFGQEVAGVMELGMLGRGEHSEITTF Sbjct: 121 SRYQEEKRVVEPKDVGPLISMKEMNRCIHCTRCVRFGQEVAGVMELGMLGRGEHSEITTF 180 Query: 181 VGKTVDSELSGNMIDLCPVGALTSKPFRYSARTWELSRRKSVSPHDSVGANLIVQVKNGK 240 VGKTVDSELSGNMIDLCPVGALTSKPFRYSARTWELSRRKSVSPHDSVGANLIVQVKNGK Sbjct: 181 VGKTVDSELSGNMIDLCPVGALTSKPFRYSARTWELSRRKSVSPHDSVGANLIVQVKNGK 240 Query: 241 VMRVLPFENEEVNECWISDKDRFAYEALNSAERLTKPMLKQDGKWQEVDWQTALEYVAHG 300 VMRVLPFENEEVNECWISDKDRFAYEAL+S +RLTKPMLKQDGKWQEVDWQTALEYVAHG Sbjct: 241 VMRVLPFENEEVNECWISDKDRFAYEALDSEQRLTKPMLKQDGKWQEVDWQTALEYVAHG 300 Query: 301 LRNIRHEHGADAIAALGTPYSTLEELNLLQKVVRGVGSENVDFRLRQSDFALDGQIKPWL 360 LRNIRHEHGADAIAALGTPYSTLEEL+LLQK+VRGVGSEN+DFRLRQSDF LDGQIKPWL Sbjct: 301 LRNIRHEHGADAIAALGTPYSTLEELSLLQKIVRGVGSENIDFRLRQSDFGLDGQIKPWL 360 Query: 361 GMSIVEFSQLNRVFVIGSFLRKDHPLLSARLRQSAKSGAKVSVLHATDDDLLMPIANKII 420 GMSIVEFSQLNRVFVIGSFLRKDHPLLSARLRQSAKSGAKVSVLHATDDDLLMP+ANKII Sbjct: 361 GMSIVEFSQLNRVFVIGSFLRKDHPLLSARLRQSAKSGAKVSVLHATDDDLLMPVANKII 420 Query: 421 AAPSAWLSALAEVAVAVAQAKGIAAPAAYAGVAASEAAKATAASLLSGDAKGVFLGNAAV 480 AAPSAWLSALAEVAVAVA+AKG+AAP+AYAGV AS+AAKATAASLLSGDAKGVFLGNAAV Sbjct: 421 AAPSAWLSALAEVAVAVAEAKGVAAPSAYAGVTASDAAKATAASLLSGDAKGVFLGNAAV 480 Query: 481 QHPQAAQLHVAAQWIAEQTGAKLGVLTEGGNAVGAYLANAVPAPGKAAAVFAQPRKAYLL 540 QHPQAAQLH AAQWIAEQTGAKLGVLTEGGNAVG YLANAVPA GKAAAVFAQPRKAYLL Sbjct: 481 QHPQAAQLHAAAQWIAEQTGAKLGVLTEGGNAVGGYLANAVPAAGKAAAVFAQPRKAYLL 540 Query: 541 LNAEPELDSYDPQAARAALNQADMVVVMSAFQHGADYADVLLPIAPFTETSGTFVNAEGR 600 LNAEPELDSYDPQAA AAL QADMVVVMSAF+HGADYADVLLPIAPFTETSGTFVN EGR Sbjct: 541 LNAEPELDSYDPQAASAALKQADMVVVMSAFKHGADYADVLLPIAPFTETSGTFVNTEGR 600 Query: 601 AQSFNGTVKPAGDARPGWKVLRVLGNLLELENFDYETSESIRDELLGKEVDLSARLNNTA 660 AQSFNGTVKPAGDARPGWKVLRVLGNLLELENF+YETSESIRDELLGKE DLSARLNN A Sbjct: 601 AQSFNGTVKPAGDARPGWKVLRVLGNLLELENFEYETSESIRDELLGKETDLSARLNNLA 660 Query: 661 AQQAQTVTAVAGSLERVADVPIYFADAIARRSPPLLETRDGQAPKAWLSAALAAQLGVAE 720 + Q + V+ A +ERVADVPIYFAD IARRS PLLETRDGQAP+AWLSAALA++LGVAE Sbjct: 661 SVQLRAVSTAAAEVERVADVPIYFADPIARRSAPLLETRDGQAPRAWLSAALASKLGVAE 720 Query: 721 GDQVTVKQGSGSAALTAAIDKKLPENVVRVAAAHASTAALGGMFGAIVVEKA 772 GDQV VKQGSGSAALTAAIDKKLPENVVRVAAAHASTAALGGMFGAIVVEKA Sbjct: 721 GDQVKVKQGSGSAALTAAIDKKLPENVVRVAAAHASTAALGGMFGAIVVEKA 772 >gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydrogenase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 957 Score = 221 bits (562), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 239/883 (27%), Positives = 370/883 (41%), Gaps = 150/883 (16%) Query: 1 MVEIEIDGKKVEVQEGSMVMDAANKIGTYIPHFCYHKKLSIAANCRMCLVEVEKAPKPL- 59 MV +EIDG V V G+ VM A+ + G +P C L +CR+CLVE+EK + + Sbjct: 19 MVTLEIDGVAVTVPAGTSVMRASVEAGINVPKLCATDSLEPFGSCRLCLVEIEKDGRKMK 78 Query: 60 --PA-CATPVNNGMIVRTASDKAVKAQKGVMEFLLINHPLDCPICDQGGECQLQDLA--V 114 PA C TP GM V+T + K + ++GVME + +HPLDC C G C+LQD+A V Sbjct: 79 GYPASCTTPCEPGMKVQTQTPKLAEIRRGVMELYISDHPLDCLTCPTNGNCELQDMAGVV 138 Query: 115 GYGGSTSRYEEEKRVVEPKDVGPLISMKEMNRCIHCTRCVRFGQEVAGVMELGMLGRGEH 174 G YE E KD + ++CI C RCVR +E G L + GRG Sbjct: 139 GLREVRYGYEGENHTKMKKDESNPYFTYDPSKCIVCNRCVRACEETQGTFALTINGRGFE 198 Query: 175 SEITTF-VGKTVDSEL--SGNMIDLCPVGALTSKPFRYSARTWELSRRKSVSPHDSVGAN 231 S ++ + ++SE G ++ CP LT K + E S+ + + + VG + Sbjct: 199 SRVSAGQMEAFMESECVSCGACVEACPTATLTEKTVIMLGQA-EHSKVTTCA-YCGVGCS 256 Query: 232 LIVQVKNGKVMRVLPFENEEVNECWISDKDRFAYEALNSAERLTKPMLKQ--DGKWQEVD 289 ++K +V+R++P+++ + N+ K RFA+ +R+ PM+++ + W+EV Sbjct: 257 FKAEMKGNEVVRMVPYKDGKANQGHSCVKGRFAWGYATHKDRILNPMIRKSINDPWREVS 316 Query: 290 WQTALEYVAHGLRNIRHEHGADAIAALGTPYSTLEELNLLQKVVR-GVGSENVDFRLRQS 348 W+ AL Y A R I+ +HG D+I + + T EE L+QK+VR G+ NVD R Sbjct: 317 WEEALSYAASEFRRIQAKHGKDSIGGITSSRCTNEETYLVQKLVRAAFGNNNVDTCARVC 376 Query: 349 DFALDGQIKPWLGMS--IVEFSQLNR---VFVIGSFLRKDHPLLSARLRQSAKSGAKVSV 403 +K LG S F + + + ++G+ HP+ ++++++ + GAK+ V Sbjct: 377 HSPTGYGLKQTLGESAGTQTFESVMKSDVILIMGANPAAGHPVFASQIKRRVRQGAKLIV 436 Query: 404 LHATDDDLLMPIANKIIAAP---------------SAWLSALAEVAVA------------ 436 + P +++ +P +A ++ALA V ++ Sbjct: 437 ID--------PRTTEMVKSPHIQADYHLKLRPGTNTAIITALAHVILSEGLQDEAYIQER 488 Query: 437 -------------VAQAKGIAAPAAYAGVAASEAAKATAASLLSGDAKGVFLGNAAVQHP 483 + + A AA GV A E + A +G ++ G +H Sbjct: 489 CDLQSYQDWKEFVLREDNSPEAMAAITGVPA-EIIRGAARLYATGGNAAIYYGLGVTEHA 547 Query: 484 QAAQLHVAAQWIAEQTG--AKLGV----LTEGGNAVGAYLANAVPA--PG----KAAAVF 531 Q + + +A TG + GV L N G+ + P PG + V Sbjct: 548 QGSTTVMGIANLAMCTGNVGREGVGVNPLRGQNNVQGSCDMGSFPHELPGYRHISDSTVR 607 Query: 532 AQPRKAY-LLLNAEPELD------------------------SYDP--QAARAALNQADM 564 Q +A+ + LN EP L DP Q AAL + Sbjct: 608 NQFEQAWGVTLNPEPGLRIPNMFDAALDGSFKGLYCEGEDIVQSDPNTQHVAAALQAMEC 667 Query: 565 VVVMSAF-QHGADYADVLLPIAPFTETSGTFVNAEGRAQSFNGTVKPAGDARPGWKVLRV 623 +VV F A YA V LP + F E GTF NAE R V P + W+V Sbjct: 668 IVVQDLFLNETAKYAHVFLPGSSFLEKDGTFTNAERRISRVR-KVMPPKAGKSDWEVTVA 726 Query: 624 LGNLLELENFDYETSESIRDELL-------GKEVDLSARLN------NTAAQQAQTVTAV 670 L L Y I DE+ G D +L N +A + + V Sbjct: 727 LAEALGYP-MPYNHPSEIMDEIAALTPSFHGVSYDKLEKLGSIQWPCNESAPEGTPIMHV 785 Query: 671 AGSLERVADVPI---YFA--DAIARRSPPLL-------------ETRDGQAPKAWLSAAL 712 GS R I YFA + + +R P +L +TR + + W S Sbjct: 786 -GSFIRGKGKFINTQYFATDEKVTQRYPLILTTGRILSQYNVGAQTRRTENSQ-WHSEDR 843 Query: 713 -------AAQLGVAEGDQVTVKQGSGSAALTAAIDKKLPENVV 748 A G+ EGD V V+ +G L A + +K+ VV Sbjct: 844 LEIHPHDAEDRGIREGDWVGVESRAGQTVLRATVTEKVQPGVV 886 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.131 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,076,486 Number of extensions: 79501 Number of successful extensions: 327 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 322 Number of HSP's successfully gapped: 2 Length of query: 772 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 679 Effective length of database: 1,179,515 Effective search space: 800890685 Effective search space used: 800890685 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)