BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1623.p (325 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [H... 633 0.0 gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [H... 134 1e-32 gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [H... 118 6e-28 gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [H... 90 3e-19 gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 62 6e-11 >gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 325 Score = 633 bits (1632), Expect = 0.0, Method: Compositional matrix adjust. Identities = 312/325 (96%), Positives = 320/325 (98%) Query: 1 MRAIEIKEFGGPEVLQACERPVPALKSGEVLIKVHAAGVNRPDVFQRTGNYPVPPGASDL 60 MRAIEIKEFGGPEVLQACERPVP LK+GEVLIKVHAAGVNRPDVFQRTGNYPVPPGASDL Sbjct: 1 MRAIEIKEFGGPEVLQACERPVPELKAGEVLIKVHAAGVNRPDVFQRTGNYPVPPGASDL 60 Query: 61 PGLEVAGEIVAGELGESGFQLGDLVCALVQGGGYAEFCAAPLAQCLPVPKGLSALEAAAL 120 PGLEVAGEIVAG+LG+SGF+LGDLVCALVQGGGYAEFCAAPLAQCLPVPKGLSALEAAAL Sbjct: 61 PGLEVAGEIVAGDLGDSGFKLGDLVCALVQGGGYAEFCAAPLAQCLPVPKGLSALEAAAL 120 Query: 121 PENYFTVWSNVFDRGQLSGEETLLVQGGSSGIGVTAIQIARALGHRVFATAGSAEKCRAC 180 PENYFTVWSNVFDRGQLSGEETLLVQGGSSGIGVTAIQIARALGHRVFATAGSAEKCRAC Sbjct: 121 PENYFTVWSNVFDRGQLSGEETLLVQGGSSGIGVTAIQIARALGHRVFATAGSAEKCRAC 180 Query: 181 EELGAERAINYKTEDFEAVVKELTDGRGVDVILDMVGGDYLPREIKALADDGRLVFIAQL 240 EELGAERAINYKTEDFEAVVKELT GRGVDVILDMVGGDYLPREIKALADDGRLVFIAQL Sbjct: 181 EELGAERAINYKTEDFEAVVKELTGGRGVDVILDMVGGDYLPREIKALADDGRLVFIAQL 240 Query: 241 GGSKGQLDMGQVMRRRLTITGSTLRPRPVAFKAAIAAKLRQHVWPLIEAGQIKPVIHQRF 300 GG+KGQLDMGQVMRRRLTITGSTLRPRPVAFKAAIAAKLR+HVWPLIEAG+I+PVIH RF Sbjct: 241 GGAKGQLDMGQVMRRRLTITGSTLRPRPVAFKAAIAAKLREHVWPLIEAGKIRPVIHHRF 300 Query: 301 PLEQAAQAHAMMESSAHIGKIMLEL 325 PLEQAAQAHAMME S HIGKIMLEL Sbjct: 301 PLEQAAQAHAMMEGSTHIGKIMLEL 325 >gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 324 Score = 134 bits (336), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 111/330 (33%), Positives = 156/330 (47%), Gaps = 18/330 (5%) Query: 2 RAIEIKEFGGPEVLQACERPVPALKSGEVLIKVHAAGVNRPDVFQRTGNYPVPPGASDLP 61 +AI + + GGPEV++ + V +GEV I+ A G+N D + R G YP P LP Sbjct: 3 KAIRMSKTGGPEVMEYVDVEVGEPGAGEVRIRHAAVGLNFIDCYFRAGLYPQP-----LP 57 Query: 62 ---GLEVAGEIVAGELGESGFQLGDLVC-ALVQGGGYAEFCAAPLAQCLPVPKGLSALEA 117 G E AG I A G SGF++GD V A G YAE P + +P +S A Sbjct: 58 AGLGQEAAGTIEAVGAGVSGFKVGDRVAYAGRPNGAYAEERVMPADILVKLPDAISFDTA 117 Query: 118 AALPENYFTVWSNVFDRGQLSGEETLLVQGGSSGIGVTAIQIARALGHRVFATAGSAEKC 177 AA+ TV + + +T+L + GIG+ A Q ARALG + T S EK Sbjct: 118 AAMMLQGMTVQYLLRQTYAVQPGDTILFHAAAGGIGLIACQWARALGVNLIGTVSSDEKA 177 Query: 178 RACEELGAERAINYKTEDFEAVVKELTDGRGVDVILDMVGGDYLPREIKALADDGRLVFI 237 + GA INYKTE+F VKELT GRGV V+ D +G D + L G +V Sbjct: 178 ALALQHGAAHVINYKTENFVERVKELTGGRGVQVVYDSIGKDTFLGSLDCLRPRGMMVSF 237 Query: 238 AQLGGSKGQLDMGQVMRRRLTITGSTLRPRP-VAFKAAIAAKLRQ---HVWPLIEAGQIK 293 G + ++ R GS RP + + A+L ++ ++ +I Sbjct: 238 GNASGPVAPFGVSELASR-----GSLFLTRPSLGNYTSTRAELDATAGDLFDMVLGNKIS 292 Query: 294 PVIHQRFPLEQAAQAHAMMESSAHIGKIML 323 I+QR+ L+ AQAHA +E G +L Sbjct: 293 IEINQRYALKDVAQAHADLEGRKTTGSTIL 322 >gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 118 bits (296), Expect = 6e-28, Method: Compositional matrix adjust. Identities = 106/353 (30%), Positives = 170/353 (48%), Gaps = 48/353 (13%) Query: 1 MRAIEIKEFGGPEVLQACERPVPALKSGEVLIKVHAAGVNRPDVFQRTGNYPVPPGASD- 59 M+AI+ FGGPEVL+ + PVP L+ GEVL++VHA G+N PD + R G +PP Sbjct: 6 MKAIQQHAFGGPEVLRYEDAPVPELQPGEVLVRVHAVGLNPPDWYLREGYKMLPPEWQPT 65 Query: 60 -----LPGLEVAGEIVAGELGESGFQLGDLVCALVQ------GG--GYAEFCAAPLAQCL 106 + G +++G + A F +GD V ++V+ GG YA++ + ++ Sbjct: 66 VAFPLILGTDISGVVEAVADDVKDFAVGDEVYSMVRFPSGLAGGSRAYAQYVSVAASEIA 125 Query: 107 PVPKGLSALEAAALPENYFTVWSNVFDRGQ--------------LSGEETLLVQGGSSGI 152 P+ +S +AAA P + T W + + G ++T++V G + G+ Sbjct: 126 LKPQRISHAQAAAAPMSLLTAWQFMIELGHDIQNPLQPHRHEPVPLKDKTVVVNGAAGGV 185 Query: 153 GVTAIQIARALGHRVFATAGSAEKCRACEELGAERAINYKTEDFEAVVKELTDGRGVDVI 212 G A+Q+A+ G RV A GS +LGA+ I+Y E V+ + VD++ Sbjct: 186 GHFALQLAKWKGARVIAI-GSGGHEAFLRDLGADEFIDYTRSAAEDVLSD------VDLV 238 Query: 213 LDMVGGDYLPREIKALADDGRLVFIAQLGGSKGQLDMGQVMRRRLTITGSTLRPRPVAFK 272 +D VGG R ++ L G L I LG S G RL + ST + R Sbjct: 239 IDAVGGPASGRFLRTLKRGGALFPIFPLGFS------GAEEAARLGVNVSTTQVR----- 287 Query: 273 AAIAAKLRQHVWPLIEAGQIKPVIHQRFPLEQAAQAHAMMESSAHIGKIMLEL 325 + A+L+Q + L+++G ++ I F L+ A +AH GKI+L + Sbjct: 288 -SSGAQLQQ-LAGLLDSGVVRVAIDSTFALQDAGKAHERAAQGHIRGKIVLSV 338 >gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 309 Score = 89.7 bits (221), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 88/331 (26%), Positives = 145/331 (43%), Gaps = 29/331 (8%) Query: 1 MRAIEIKEFGGPEVLQACERPVPALKSGEVLIKVHAAGVNRPDVFQRTG--NYPVPPGAS 58 MRAI ++G P VL C+ L G V + + AAGV D R G + V Sbjct: 1 MRAICYHQYGPPSVLHLCDLDEQPLPPGHVRVAMQAAGVAPVDTKLRAGRLQHLVALDLP 60 Query: 59 DLPGLEVAGEIVAGELGESGFQLGDLVCALVQGGGYAEFCAA---PLAQCLPVPKGLSAL 115 +PG + AG I A G Q+G+ VC + G A P Q + P LS Sbjct: 61 KVPGRDGAGVITALGEGVHTLQVGEAVCVMPSMAGRGTTLAELVLPQEQVVRKPDVLSMQ 120 Query: 116 EAAALPENYFTVWSNVFDRGQLSGEETLLVQGGSSGIGVTAIQIARALGHRVFATAGSAE 175 +AAAL + + W + ++ + + GS +G +Q+A LG V A+ S+ Sbjct: 121 QAAALLQPACSAWIALMQTAPIAAGMKVFIHAGSGAVGSLMVQLAAHLGAEVTASCRSS- 179 Query: 176 KCRACEELGAERAINYKTEDFEAVVKELTDGRGVDVILDMVGGDYLPREIKALADDGRLV 235 LGA R + Y + EL R DV++D+VGG + + L G+LV Sbjct: 180 NVEYVAALGASRVLAYDRD-------ELGRVRDQDVVIDLVGGAAHDQSYRMLRRGGQLV 232 Query: 236 FIAQLGGSKGQLDMGQVMRRRLTITGSTLRPRPVAFKAAIAAKLRQHVWPLIEAGQIKPV 295 ++ + + G +RR L + ++ + +AA +Q VW +P Sbjct: 233 WLVAAPIRERGEEFGVSVRRALIVEDQSILQQ-------VAALAQQGVW--------RPA 277 Query: 296 IHQRFPLEQAAQAHAMMES-SAHIGKIMLEL 325 + P+++ A+AH +E+ G+++L++ Sbjct: 278 VSGVLPMQEIARAHQRIEAGEVTRGRLVLDI 308 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 62.0 bits (149), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 63/234 (26%), Positives = 100/234 (42%), Gaps = 32/234 (13%) Query: 1 MRAIEIKEFGGPEVLQACERPVPALKSGEVLIKVHAAGVNRPDVFQRTGNYPVPPGASDL 60 M+A ++EFG P ++ + PVP G++L+K A+GV D+ G++PV P + Sbjct: 5 MKAAVVREFGKPLSIE--QVPVPTPAPGQILVKFEASGVCHTDLHAAHGDWPVKPTPPFI 62 Query: 61 PGLEVAGEIVAGELGESGFQLGDLV----------------------CALVQGGGY---- 94 PG E G + A G + GD V CA Q GY Sbjct: 63 PGHEGTGYVAAVGAGVKHVKEGDRVGVPWLHTACGCCSPCRTGWETLCAEQQNTGYSVNG 122 Query: 95 --AEFCAAPLAQCLPVPKGLSALEAAALPENYFTVWSNVFDRGQLSGEETLLVQGGSSGI 152 AE+ A +P L AA + TV+ + + GE +V G G+ Sbjct: 123 SFAEYGLADPKFVGHLPDNLDFGPAAPVLCAGVTVYKGLKETEVRPGE--WVVISGIGGL 180 Query: 153 GVTAIQIARALGHRVFATAGSAEKCRACEELGAERAINYKTEDFEAVVKELTDG 206 G A+Q A+A+G V A +K ++LGA+ ++ + + A V+ + G Sbjct: 181 GHMAVQYAKAMGMHVVAADIHEDKLALAKKLGADLTVDGRNHNAVAEVQRIIGG 234 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,120,315 Number of extensions: 52099 Number of successful extensions: 199 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 185 Number of HSP's successfully gapped: 7 Length of query: 325 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 240 Effective length of database: 1,217,395 Effective search space: 292174800 Effective search space used: 292174800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)