BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1612.p (174 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311082|ref|YP_003775174.1| transmembrane protein [Herbaspi... 298 1e-82 gi|300312390|ref|YP_003776482.1| lipoprotein [Herbaspirillum ser... 59 2e-10 >gi|300311082|ref|YP_003775174.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 177 Score = 298 bits (764), Expect = 1e-82, Method: Compositional matrix adjust. Identities = 154/171 (90%), Positives = 158/171 (92%), Gaps = 3/171 (1%) Query: 7 LFQRIRPRLAAALLAAASLA---GCASLLQPPVQPGEPEDQVVARLGPPTNVYPDGNVRV 63 L Q RPRLAAALLA ++L GCASLLQPPVQPGEPEDQVVARLGPPTNVYPDGNVRV Sbjct: 7 LLQSTRPRLAAALLALSALCALSGCASLLQPPVQPGEPEDQVVARLGPPTNVYPDGNVRV 66 Query: 64 FEYAAGAFGQYQYLARIGPDHRLISYAQVWTIENFRAIRPKQDTKLDVLRRVGRPTEVTR 123 FEY AG F QYQY+ARIGPDHRLISY QVWTIENFRAIRPKQDTKLDVLRRVGRPTEVTR Sbjct: 67 FEYGAGPFSQYQYMARIGPDHRLISYEQVWTIENFRAIRPKQDTKLDVLRRVGRPTEVTR 126 Query: 124 YARIPFEAWNYGYKESGVWNSQMTVYFNDQGIVEKVENGPDPRFDDSRFRF 174 YARIPFEAWNYGYKESGVWNSQMTVYF DQGIVEKVENGPDPR+DDSRFRF Sbjct: 127 YARIPFEAWNYGYKESGVWNSQMTVYFTDQGIVEKVENGPDPRYDDSRFRF 177 >gi|300312390|ref|YP_003776482.1| lipoprotein [Herbaspirillum seropedicae SmR1] Length = 175 Score = 58.9 bits (141), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 45/141 (31%), Positives = 61/141 (43%), Gaps = 12/141 (8%) Query: 7 LFQRIRPRLA--AALLAAASLAGCASLLQP-------PVQPG-EPEDQVVARLGPPTNVY 56 F R+R A L A ++ GC +P +Q G E V +G P V+ Sbjct: 5 FFTRLRRHAGKFGAFLVALAMLGCDRNGKPIEEFGLDKLQKGISSEADVRDVMGQPDTVW 64 Query: 57 PDG-NVRVFEYAAGAFGQYQYLARIGPDHRLISYAQVWTIENFRAIRPKQDTKLDVLRRV 115 DG R EY G G ++ IG LI Y QV T E+F IR T + R+ Sbjct: 65 DDGQGARTLEYPKGPQGIRTWMFSIGASGTLIDYQQVLTQEHFDTIRAGM-TAEQIRRQF 123 Query: 116 GRPTEVTRYARIPFEAWNYGY 136 GRP V ++ R E W++ Y Sbjct: 124 GRPRSVVQFGRKNEEVWDWKY 144 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 576,737 Number of extensions: 23307 Number of successful extensions: 33 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 33 Number of HSP's successfully gapped: 2 Length of query: 174 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 95 Effective length of database: 1,245,805 Effective search space: 118351475 Effective search space used: 118351475 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)