BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1609.p (496 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311079|ref|YP_003775171.1| N-acyl-D-glutamate deacylase [H... 947 0.0 gi|300309905|ref|YP_003773997.1| N-acyl-D-glutamate deacylase [H... 446 e-126 gi|300309903|ref|YP_003773995.1| N-acyl-D-glutamate deacylase [H... 176 4e-45 >gi|300311079|ref|YP_003775171.1| N-acyl-D-glutamate deacylase [Herbaspirillum seropedicae SmR1] Length = 496 Score = 947 bits (2449), Expect = 0.0, Method: Compositional matrix adjust. Identities = 454/495 (91%), Positives = 474/495 (95%) Query: 1 MISTTHTPEGQRRCDTLIRHALVIDGSGADPVTADVAVCEGRIAAIGQLDQWQATQEIDA 60 M+STTH +G+RRCD LIRHALVIDGSGA+PV ADVAV +GR+AAIG+LD WQA QEIDA Sbjct: 1 MLSTTHAADGERRCDILIRHALVIDGSGAEPVAADVAVHKGRVAAIGKLDDWQAAQEIDA 60 Query: 61 RGRVLAPGFIDVHTHDDTNVIRTPEMFPKLSQGVTTVVVGNCGISAAPVLLKGEPPDPMN 120 GRVL+PGFIDVHTHDDTNVIRTPEMFPKLSQGVTTVVVGNCGISAAPV LKGEPPDPMN Sbjct: 61 SGRVLSPGFIDVHTHDDTNVIRTPEMFPKLSQGVTTVVVGNCGISAAPVTLKGEPPDPMN 120 Query: 121 LLGKAEAFHYPSFASYVEAVNAAQPSINVAALVGHTALRNNQMDRLDRAATESEIVAMRA 180 LLGK EAFHYPSFASYVEAVNAA+P+INVAALVGHTALRNN MDRLDRAATESEI MRA Sbjct: 121 LLGKREAFHYPSFASYVEAVNAARPAINVAALVGHTALRNNHMDRLDRAATESEIAGMRA 180 Query: 181 QLREALEHGALGLSTGLAYGNAINAPTAEVLALAEPLAEFGGLYATHLRSEFADILEAME 240 QLREALEHGALGLSTGLAYGNAINAPT EVLALAEPLAEFGGLYATHLRSEFADIL+AME Sbjct: 181 QLREALEHGALGLSTGLAYGNAINAPTEEVLALAEPLAEFGGLYATHLRSEFADILDAME 240 Query: 241 EAFRIGKHARVPVVISHLKCAGVENWGRSSEVLQALEAAARHQHVGCDCYPYTASSSTLD 300 E+FRIGKHARVPVVISHLKCAGVENWGRSSEVL ALE AARHQHVGCDCYPYTASSSTLD Sbjct: 241 ESFRIGKHARVPVVISHLKCAGVENWGRSSEVLHALERAARHQHVGCDCYPYTASSSTLD 300 Query: 301 LKQVTSDYDIQVTWSEPHPEQGGKMLQQIAADWGVDLHTAAARLMPAGAVYHCMSDDDVN 360 LKQVT DYDIQVTWSEPHPEQGGKML+QIAA+W VDLH AAARLMPAGAVYHCMSDDDVN Sbjct: 301 LKQVTGDYDIQVTWSEPHPEQGGKMLKQIAAEWDVDLHAAAARLMPAGAVYHCMSDDDVN 360 Query: 361 RILSHPATVVGSDGLPNDPLPHPRLWGAFPRVLGYYSREQKLFSLATAVHKMTGLSAQRF 420 RILSHPATVVGSDGLPNDPLPHPRLWGAFPRVLGYYSREQKLFSLATAVHKMTGLSAQRF Sbjct: 361 RILSHPATVVGSDGLPNDPLPHPRLWGAFPRVLGYYSREQKLFSLATAVHKMTGLSAQRF 420 Query: 421 GLELRGFVREGYHADLVLFDADTIRDAASFTDPMQPAHGIEAVWVNGQLAYRGQDKQATS 480 GL+LRGFVR GYHADLVLFDA+T+RDAASFTDPMQPAHGIEAVWVNG+LAYRGQDK+AT+ Sbjct: 421 GLDLRGFVRVGYHADLVLFDAETVRDAASFTDPMQPAHGIEAVWVNGELAYRGQDKKATA 480 Query: 481 ARAGRFLARMVQKTS 495 ARAGRFLAR VQKTS Sbjct: 481 ARAGRFLARTVQKTS 495 >gi|300309905|ref|YP_003773997.1| N-acyl-D-glutamate deacylase [Herbaspirillum seropedicae SmR1] Length = 498 Score = 446 bits (1146), Expect = e-126, Method: Compositional matrix adjust. Identities = 242/478 (50%), Positives = 310/478 (64%), Gaps = 8/478 (1%) Query: 15 DTLIRHALVIDGSGADPVTADVAVCEGRIAAIGQLDQWQATQEIDARGRVLAPGFIDVHT 74 D LI +IDGS A AD+ V GRIA IG L QA Q IDA G+++APGFID HT Sbjct: 8 DLLITGGTIIDGSKAPRFRADIGVRHGRIAEIGNLSHRQAAQRIDASGKIVAPGFIDSHT 67 Query: 75 HDDTNVIRTPEMFPKLSQGVTTVVVGNCGISAAPVLLKGEP-PDPMNLLGKAEAFHYPSF 133 HDD V+ P+M K+SQGVTTV+ GNCGIS AP L G P P P++LL A+ + +F Sbjct: 68 HDDQAVLSQPDMTFKISQGVTTVITGNCGISIAP-LRAGTPLPAPLSLLDDADGTRFETF 126 Query: 134 ASYVEAVNAAQPSINVAALVGHTALRNNQMDRLDRAATESEIVAMRAQLREALEHGALGL 193 +SY++A+ ++NVAA+VGH+ LR MD L+R A EI AM A L EALE GA+G+ Sbjct: 127 SSYLDALRQTPSAVNVAAMVGHSTLRVMTMDALEREARPDEISAMNALLDEALEAGAIGI 186 Query: 194 STGLAYGNAINAPTAEVLALAEPLAEFGGLYATHLRSEFADILEAMEEAFRIGKHARVPV 253 STG Y A +A T EV+ + P+A G LY TH+R E +EA+EE+F IG+ PV Sbjct: 187 STGTYYPPAAHASTQEVIDVCRPIAGTGALYVTHMRDEADKSMEALEESFLIGRELNAPV 246 Query: 254 VISHLKCAGVENWGRSSEVLQALEAAARHQHVGCDCYPYTASSSTL--DLKQVTSDYDIQ 311 VISH K N+G+SS+ L + A + Q V DCYPY ASS+ L D K++ I Sbjct: 247 VISHHKLQRAHNFGKSSQTLARIRQAMQCQCVALDCYPYNASSTMLHRDPKRLVG--RIL 304 Query: 312 VTWSEPHPEQGGKMLQQIAADWGVDLHTAAARLMPAGAVYHCMSDDDVNRILSHPATVVG 371 ++ S+PHPEQ G+ L IAA+WGV AAARL PA AVY M + DV IL+ T++G Sbjct: 305 ISSSKPHPEQAGRELDAIAAEWGVSKEEAAARLQPASAVYFGMDESDVRNILAFDETMIG 364 Query: 372 SDGLPNDPLPHPRLWGAFPRVLGYYSREQKLFSLATAVHKMTGLSAQRFGLELRGFVREG 431 SDG+P PHPRLWG FPR+LG+YSR+ LF L AV KMTGL+A+ F L RG + G Sbjct: 365 SDGIPTGEKPHPRLWGTFPRILGHYSRDLGLFDLEVAVWKMTGLTARNFKLHERGTLEVG 424 Query: 432 YHADLVLFDADTIRDAASFTDPMQPAHGIEAVWVNGQLAYRGQDKQATSARAGRFLAR 489 ++AD+V+FDA TIRD AS+ P +PA GI+AV VNG + +R Q ARAG +AR Sbjct: 425 HYADIVVFDAATIRDTASYETPAEPAQGIDAVIVNGVVTWRL--GQHMGARAGHVIAR 480 >gi|300309903|ref|YP_003773995.1| N-acyl-D-glutamate deacylase [Herbaspirillum seropedicae SmR1] Length = 550 Score = 176 bits (446), Expect = 4e-45, Method: Compositional matrix adjust. Identities = 147/529 (27%), Positives = 242/529 (45%), Gaps = 87/529 (16%) Query: 13 RCDTLIRHALVIDGSGADPVTADVAVCEGRIAAIGQLDQ--WQATQEIDARGRVLAPGFI 70 + D +I+ + DG +P++ ++ + RI +G+ D+ + A + IDARG ++APGFI Sbjct: 47 QLDLIIQSDRIYDGEHDEPLSGELGIAGDRIVYVGKADRSRFTALRVIDARGAIVAPGFI 106 Query: 71 DVHTHDD----TNVIRTPEMFPKLSQGVTTVVVGNCGISAAPVLLKGEPPDPMNLLGKAE 126 D HTH D T+ I+ ++ L+QGVTT V+GN G Sbjct: 107 DAHTHADRDMFTDDIKKRQVDYFLTQGVTTAVIGNDG----------------------- 143 Query: 127 AFHYPSFASYVEAVNAAQPSI-----NVAALVGHTALRNNQMDRLDRAATESEIVAMRAQ 181 SY A AA S N A VG +R +Q+ D+ + ++ AM+A Sbjct: 144 ------GGSYEVAQQAAWLSRHGIGPNAAMYVGFGPIRRSQLGEADQQPDQKQLEAMKAL 197 Query: 182 LREALEHGALGLSTGLAYGNAINAPTAEVLALAEPLAEFGGLYATHLRSEFA---DILEA 238 + + + GA+G STGL Y A T EV+ALA+ A++GG+Y +H+R E + + E+ Sbjct: 198 VAKGMCEGAIGFSTGLFYAPQNFASTGEVIALAKVAAQYGGIYDSHIRDEASYNVGLAES 257 Query: 239 MEEAFRIGKHARVPVVISHLKCAGVENWGRSSEVLQALEAA-ARHQHVGCDCYPYTASSS 297 + E RI + A + ++H+K G G+S +++ +EAA A+ + D YP+ ASS+ Sbjct: 258 IREVIRIAREAGIAANVAHIKALGKSVEGQSGAIIRMIEAARAQGLRITADQYPWLASST 317 Query: 298 TLD-----------------------LKQVTSDYDI-----------QVTWSEPHPEQGG 323 +Q DI + +S+ +P+ G Sbjct: 318 RFSAALIPPWALDGGRPAMLRRFDDPAQQQRLHRDIGENLRLRNGAGAILFSQGNPKYVG 377 Query: 324 KMLQQIAADWGVDLHTAAARLMPAGAVY---HCMSDDDVNRILSHPATVVGSDGLPNDPL 380 K L+Q+A + D +AA ++ + S+ DV + P + SD Sbjct: 378 KTLEQLAQERNTDPVSAAIEVLRDSEIVIASFNQSNKDVVAFMKRPWVMTSSDSFHG--- 434 Query: 381 PHPRLWGAFPRVLGYYSREQKLFSLATAVHKMTGLSAQRFGLELRGFVREGYHADLVLFD 440 HPR +F Y R Q +LA + T A G+ RG ++ G+ AD+V+FD Sbjct: 435 -HPRTVASFAEKYHEYVRNQHTITLAQFIRSSTSYPADALGIARRGRLQPGHFADVVVFD 493 Query: 441 ADTIRDAASFTDPMQPAHGIEAVWVNGQLAYRGQDKQATSARAGRFLAR 489 A++ + + + G+ A VNG+L + + T+A GR L R Sbjct: 494 PIAYAPRATYVNNEERSVGVVATVVNGELVV--DEGRLTNAMPGRPLLR 540 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,607,656 Number of extensions: 69237 Number of successful extensions: 240 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 237 Number of HSP's successfully gapped: 5 Length of query: 496 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 407 Effective length of database: 1,198,455 Effective search space: 487771185 Effective search space used: 487771185 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)