BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1606.p (310 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311076|ref|YP_003775168.1| sugar kinase,ribokinase family ... 489 e-139 gi|300310831|ref|YP_003774923.1| 2-ketogluconate kinase [Herbasp... 110 2e-25 gi|300310353|ref|YP_003774445.1| ribokinase [Herbaspirillum sero... 92 6e-20 gi|300311742|ref|YP_003775834.1| transferase kinase [Herbaspiril... 67 1e-12 gi|300311612|ref|YP_003775704.1| sugar kinase [Herbaspirillum se... 59 5e-10 >gi|300311076|ref|YP_003775168.1| sugar kinase,ribokinase family protein [Herbaspirillum seropedicae SmR1] Length = 308 Score = 489 bits (1258), Expect = e-139, Method: Compositional matrix adjust. Identities = 260/303 (85%), Positives = 277/303 (91%) Query: 1 MSKPILAYGEAMVEFNQRIDAPRQYLQGYGGDTSNFAIAAARQGAATAYLSAVGDDHFGA 60 MSK ILAYGEAMVEFNQRIDAPRQ+LQGYGGDTSNFAIAAARQGAA+AYLSAVGDDHFG Sbjct: 1 MSKTILAYGEAMVEFNQRIDAPRQFLQGYGGDTSNFAIAAARQGAASAYLSAVGDDHFGQ 60 Query: 61 DLLDLWRREQVDVQHVAIHAGGSTGLYFVTHDQAGHHFHYRRTGSAASRYAASDLPLPAI 120 DLL LW+RE+VDV+HVAI GGSTG+YFVTHD AGHHFHYRR GSAAS Y A LPL AI Sbjct: 61 DLLALWQREEVDVRHVAIAPGGSTGIYFVTHDAAGHHFHYRRAGSAASAYQAKALPLEAI 120 Query: 121 AGAGALHLSGISLAISEAACEAGLTAMAHARAAGVTTSLDTNLRLRLWSLERAREKMEQA 180 A AGALHLSGISLAISE+ACEAGL AMAHARA+GVTTSLDTNLRLRLW LERAR +ME+A Sbjct: 121 AAAGALHLSGISLAISESACEAGLAAMAHARASGVTTSLDTNLRLRLWPLERARARMEEA 180 Query: 181 LRLCDICLPSWDDVATLTGLEDRDAIVDRLLAYGVKLVAFKLGHEGCYVATPEVRRMVAP 240 RLCDICLPSW+DV+ LTGLEDRDAIVDRLL+YGVKLVAFKLG EGCYVAT + RRMVAP Sbjct: 181 FRLCDICLPSWEDVSLLTGLEDRDAIVDRLLSYGVKLVAFKLGKEGCYVATSDQRRMVAP 240 Query: 241 YPVQALDATGAGDCFGGAFMAELMAGRDPFEAARYANVAAALSTTAYGAVDPIPRRAQVQ 300 YPV +LDATGAGDCFGGAFMAELMAGRD F AARYANVAAALSTT YGAV+PIPRRAQV+ Sbjct: 241 YPVDSLDATGAGDCFGGAFMAELMAGRDAFAAARYANVAAALSTTGYGAVEPIPRRAQVE 300 Query: 301 AAL 303 A L Sbjct: 301 AVL 303 >gi|300310831|ref|YP_003774923.1| 2-ketogluconate kinase [Herbaspirillum seropedicae SmR1] Length = 313 Score = 110 bits (274), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 94/310 (30%), Positives = 131/310 (42%), Gaps = 8/310 (2%) Query: 1 MSKPILAYGEAMVEFNQRIDAPRQYLQGY----GGDTSNFAIAAARQGAATAYLSAVGDD 56 M ++ +GEA+ P + ++ Y G +N AI AR G + S VG+D Sbjct: 3 MQLDVVTWGEALALLVADEVGPFEEVEKYTRRLAGAETNVAIGLARLGLKVGWASRVGND 62 Query: 57 HFGADLLDLWRREQVDVQHVAIHAGGSTGLYFVTHDQAGHH--FHYRRTGSAASRYAASD 114 FG + +E V+V V T + G Y R GSAAS + D Sbjct: 63 AFGRFIRQRVAQEGVEVSRVITDMEFRTAIQLKAKAVGGADPAIEYYRKGSAASHLSVDD 122 Query: 115 LPLPAIAGAGALHLSGISLAISEAACEAGLTAMAHARAAGVTTSLDTNLRLRLWSLERAR 174 A LH +GI+ A+S AM R G T S D NLR LW + Sbjct: 123 FDAGYFGAARHLHATGIAPALSATTMAFAHQAMDFMRGQGKTISFDPNLRPMLWPSQEVM 182 Query: 175 EKMEQALRL-CDICLPSWDDVATLTGLEDRDAIVDRLLAYGVKLVAFKLGHEGCYVATPE 233 + AL D LP + LTG +D I L GVKLV KLG EG Y + Sbjct: 183 AQQLNALAFKADWVLPGLSEGKILTGHDDAREIAGFYLERGVKLVVIKLGAEGAYWRNGQ 242 Query: 234 VRRMVAPYPV-QALDATGAGDCFGGAFMAELMAGRDPFEAARYANVAAALSTTAYGAVDP 292 VA PV + +D GAGD F ++ ++ G +A N A + G ++ Sbjct: 243 GEGRVAGVPVKEVVDTVGAGDGFAVGVISGMLEGLPVPQAVMRGNRIGAFAIQVVGDMEG 302 Query: 293 IPRRAQVQAA 302 +P RA+++AA Sbjct: 303 LPTRAELEAA 312 >gi|300310353|ref|YP_003774445.1| ribokinase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 91.7 bits (226), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 93/285 (32%), Positives = 125/285 (43%), Gaps = 20/285 (7%) Query: 25 YLQGYGGDTSNFAIAAARQGAATAYLSAVGDDHFGADLLDLWRREQVDVQHVAIHAGGST 84 + G GG SN A+AAAR GA ++SA+G D FG L LWRRE + HV + G T Sbjct: 39 FAMGPGGKGSNQAVAAARVGAPVRFISAIGKDAFGDFALSLWRRETI-APHVRVIDGAPT 97 Query: 85 GLYFVTHDQAGHHFHYRRTGSAASRYAASDLPLPAIAGAGALHLSGISLAISEAACEAGL 144 G F+ + A AAS+ + D+ A+ + + + E EA L Sbjct: 98 GAAFIYVNDASGDNAIIVVPGAASQLSEQDVERE----RQAIEAAKVFVTQLEQPPEAAL 153 Query: 145 TAMAHARAAGVTTSLDTNLRLRLWSLERAREKMEQALRLCDICLPSWDDVATLTGLE--- 201 + ARAAG TT + L + Q LCD P+ + A LTG+E Sbjct: 154 AGLQIARAAGTTTVFNPAPALAFPA---------QVYGLCDFITPNEHEAALLTGIEIHH 204 Query: 202 --DRDAIVDRLLAYGVKLVAFKLGHEGCYV-ATPEVRRMVAPYPVQALDATGAGDCFGGA 258 D D LL GV LG +G + + + + A ++ GAGD F GA Sbjct: 205 IDDARRAADVLLERGVGCALITLGAQGSLLHSRSQSLHLPALCAGTVVETAGAGDAFNGA 264 Query: 259 FMAELMAGRDPFEAARYANVAAALSTTAYGAVDPIPRRAQVQAAL 303 F A L G EAAR A AA+S T G +P R + A L Sbjct: 265 FAAGLAEGMSAEEAARLATAVAAISVTRPGTAPSMPSRQEALALL 309 >gi|300311742|ref|YP_003775834.1| transferase kinase [Herbaspirillum seropedicae SmR1] Length = 657 Score = 67.4 bits (163), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 79/318 (24%), Positives = 127/318 (39%), Gaps = 29/318 (9%) Query: 15 FNQRIDAPRQYLQGY----GGDTSNFAIAAARQGAATAYLSAVGDDHFGADLLDLWRREQ 70 + Q++ AP + + GG ++N A AR G +A L+ +GD+ G L E Sbjct: 34 YAQQVGAPLSDVSSFAKYLGGSSANIAFGCARLGLKSAMLARIGDEANGQFLKAELAAEG 93 Query: 71 VDVQHVAIHAGGSTGLYFV-THDQAGHHFHYRRTGSAASRYAASDLPLPAIAGAGALHLS 129 DV HV + T L + D+ + R A A D+ IA + AL ++ Sbjct: 94 CDVSHVTVDPQRLTALVMLGLKDKDSFPLIFYRDNCADMALAPEDIDEAFIASSKALLIT 153 Query: 130 GISLAISEAACEAGLTAMAHARAAGVTTSLDTNLRLRLWSL-------------ERAREK 176 G + + + L A+ +AR V T LD + R LW L + + Sbjct: 154 GTHFSTPQVHRSSSL-ALQYARRHNVRTVLDIDYRPVLWGLTSKGDGETRFIASDGVTQH 212 Query: 177 MEQALRLCDICLPSWDDVATLTGLEDRDAIVDRLLAYGVKLVAFKLGHEGCYV---ATPE 233 ++ L D+ + + ++ G D A + + A + K G GC V A PE Sbjct: 213 LQGILPQFDLVVGTEEEFMIAGGSTDIMAALRAVRALCAATLVVKRGPLGCVVLHGAIPE 272 Query: 234 VRRMVAPY-----PVQALDATGAGDCFGGAFMAELMAGRDPFEAARYANVAAALSTTAYG 288 R+ + V+ L+ GAGD F F+ + D RYAN AL + + Sbjct: 273 --RLDDGFHYRGVQVEVLNVLGAGDAFISGFLKGWLNEEDDEHCCRYANACGALVVSRHA 330 Query: 289 AVDPIPRRAQVQAALQAA 306 +P ++ LQ A Sbjct: 331 CAPAMPGPIELDYFLQHA 348 >gi|300311612|ref|YP_003775704.1| sugar kinase [Herbaspirillum seropedicae SmR1] Length = 299 Score = 58.9 bits (141), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 84/283 (29%), Positives = 123/283 (43%), Gaps = 22/283 (7%) Query: 24 QYLQGYGGDTSNFAIAAAR---QGAATAYLSAVGDDHFGADLLDLWRREQVDVQHVAIHA 80 +++ GG +N A+A AR G A ++ VGDD FG + +D +++ A Sbjct: 30 RFMTIPGGKGANQAVACARLAAPGTRVAMVACVGDDAFGGQMRQSITACGIDDRYIDEVA 89 Query: 81 GGSTGLYFVTHDQAGHHFHYRRTGSAASRYAASDLPLPAIAGAGAL-HLSGISLAISEAA 139 G +TG+ + D + G A L + I A AL + I L E Sbjct: 90 GEATGIASIMVDANAQNSIVIAAG------ANGRLDVERIERARALIEQASIVLLQLEVP 143 Query: 140 CEAGLTAMAHARAAGVTTSLDTNLRLRLWSLERAREKMEQALRLCDICLPSWDDVATL-- 197 + ++ A A G T L+ +L RA L+ D + + + A L Sbjct: 144 MATVIHSIELAHALGKTVVLNPA---PAQALPRA------LLQKIDYLILNEIEAAMLAE 194 Query: 198 TGLEDRDAIVDRLLAYGVKLVAFKLGHEGCYVATPE-VRRMVAPYPVQALDATGAGDCFG 256 ED + +L G + V LG +G Y + + +R + VQA+D T AGD F Sbjct: 195 EQSEDIPMLARKLHDLGARNVVVTLGEKGVYGSFADGQQRHLPARKVQAVDTTAAGDTFI 254 Query: 257 GAFMAELMAGRDPFEAARYANVAAALSTTAYGAVDPIPRRAQV 299 G F+ + GRD FEA YA AAALS T GA IP R +V Sbjct: 255 GGFIGAIAQGRDQFEAIAYAQAAAALSVTRVGAQTSIPTRDEV 297 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.133 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 897,838 Number of extensions: 34579 Number of successful extensions: 130 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 123 Number of HSP's successfully gapped: 5 Length of query: 310 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 225 Effective length of database: 1,217,395 Effective search space: 273913875 Effective search space used: 273913875 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)